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| 1 | Isolation and characterization of insecticidal activity of (Z)- asarone from Acorus calamus L.显示文摘Acorus 省藤属的植物 L 的杀虫活性。对 Sitophilus zeamais Motschulsky 的成年人的导出根茎的材料被使用抵抗性方法和接触毒性检验。A 的生物学上活跃的成分。省藤属的植物根茎被分开并且识别。结果证明乙醇 A 提取。省藤属的植物有强壮的抵抗性和接触效果到 S。zeamais 和 A 的活跃成分。省藤属的植物被分光镜的分析作为(Z)-asarone 描绘。从测试的回答与暴露时间和剂量变化了。在抵抗性测试,乙醇 A 提取。省藤属的植物在 629.08 og/cmpo 有 93.92% 抵抗性但是仅仅 71.38% 在 157.27 og/cmpo 在处理以后的 12 h。作为一个对比,(Z)-asarone 在 314.54 og/cmpo 显示出 84.50% 抵抗性并且 77.02% 在 78.63 og/cmpo 在处理以后的 12 h。在滤纸散开测试,乙醇 A 提取。省藤属的植物引起了 95.56% 和 17.78% 死亡到 S。在 314.54 og/cmpo 和 78.63 og/ cmpo 的 zeamais 在处理以后的 4 天,当(Z)-asarone 分别地在 40.89 og/cmpo 和 15.73 og/cmpo 引起了 100.00% 和 15.56% 死亡时。这些结果显示 A 的杀虫活性。省藤属的植物摘录可能由于(Z)-asarone。 | Yingjuan Yao Wanlun Cai Changju Yang Dong Xue Yanzhang Huang | 2008 | Insect Science2008，15，3： | 22 |
| 2 | Relationship between genetic polymorphisms of metabolizing enzymes CYP2E1, GSTM1 and Kazakh's esophageal squamous cell cancer in Xinjiang, China显示文摘AIM: To analyze the relationship between genetic polymorphisms of metabolizing enzymes CYP2E1, GSTM1 andKazakh's esophageal squamous cell cancer in China.METHODS: The genotypes of cytochromes P450 (CYP) 2E1 and glutathione S-transferase (GST) M1 were investigated by polymerase chain reaction (PCR) and restriction fragment length polymorphism (RFLP) following PCR in 104 Kazakh's patients with esophageal cancer (EC) and 104 non-cancer controls.RESULTS: The frequency of CYP2E1 c1/c1 genotype was significantly higher in patients with cancer (77.9%) thanin control subjects (24.0%) (P<0.05; OR, 11.13; 95%CI,5.84-21.22). The difference of GSTM1 null was significantly more frequent in the cancer (34.6%) vsthe control group (3.8%) (P<0.05; OR, 13.24; 95%CI, 4.50-38.89). On the other hand, the combination of GSTM1 presence and CYP2E1 c1/c1 genotypes increased the risk for cancer (P<0.05;OR, 13.42; 95%CI, 6.29-28.3).CONCLUSION: The CYP2E1 c1/c1, GSTM1 deletion genotypes are genetically susceptible biomarkers for ESCC in Kazakh population. Individuals with allele c1 of RsaI polymorphic locus for CYP2E1 may increase the risk of ESCC. Moreover, CYP2E1 wild type (c1/c1) increased thesusceptibility to ESCC risk in Kazakh individuals with GSTM1 presence genotype. | Xiao-MeiLu Yue-MingZhang Ren-YongLin ArziGul XingWang Ya-LouZhang YanZhang YanWang HaoWen | 2005 | World Journal of Gastroenterology2005，11，24： | 15 |
| 3 | p53 polymorphism in human papillomavirus-associated Kazakh's esophageal cancer in Xinjiang,China显示文摘AIM: To investigate the relationship between p53 codon 72 polymorphism and human papillomavirus (HPV) type 16 infection in Kazakh's esophageal cancer (EC) in Xinjiang, China.METHODS: Encoding regions of p53codon 72 and HPV-16 E6 were amplified by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) and polymerase chain reaction (PCR) methods using pairs of primary esophageal squamous cell carcinoma (SCC) tissue and corresponding normal mucosa, which were collected from 104 patients of Kazakh in Xinjiang, China. RESULTS: Only arginine allele was detected in 70.1% (39/55) of HPV-16-E6-positive cases but only in 40.8% (20/49) of HPV-16-E6-negative cases (P<0.05; OR, 3.53; 95% CI, 1.57-7.98). In contrast, such a significant correlation between p53 polymorphism and HPV infection was not evident in corresponding normal mucosae. The allele frequency of Arg allele in cancer cases (0.68) was higher than that in normal mucosa samples (0.54) (P<0.05; OR, 1.80; 95% CI, 1.21-2.69).CONCLUSION: p53 codon 72 Arg homozygous genotype is one of the high-risk genetic factors for HPV-associated SCC of Kazakh. Individuals carrying Arg allele compared to those with Pro allele have an increased risk for esophageal SCC. | Xiao-MeiLu Yue-MingZhang Ren-YonaLin Xiao-HuiLiang Ya-LouZhang XingWang YanZhang YanWang HaoWen | 2004 | World Journal of Gastroenterology2004，10，19： | 14 |
| 4 | Comparative proteome analysis of untreated and Helicobacter pylori-treated HepG2显示文摘AIM: To investigate the pathological effect of Helicobacter pylori(H pylori) on human hepatic ceils, proteomic methods were used to find and to identify proteins that were overexpressed in HepG2 cells treated by H pylori.METHODS: H pylori was co-cultured with HepG2 for 6 h. Two-dimensional gel electrophoresis was used to gainthe protein expression pattern of untreated and H pyloritreated HepG2. After staining and image analysis,spots of interest were isolated and subjected to massspectrometry.RESULTS: Seven proteins, which were up-regulated inH p ylori-treated HepG2 cells, were identified. These proteinsincluded integrin beta-1, protein kinase C alpha, LIM/ homeobox protein Lhx1, eIF-2-beta, MAP kinase kinase 3, PINCH protein and Ras-related protein Rab-37, which involved in transcription regulation, signal transduction, metabolism and so on.CONCLUSION: H pylori may exert the pathological effect on HepG2 cells by up-regulating the expression of some proteins. | YanZhang Xue-GongFan RenChen Zhi-QiangXiao Xue-PingFeng Xue-FeiTian Zhao-HuiChen | 2005 | World Journal of Gastroenterology2005，11，22： | 14 |
| 5 | Effects of sodium β-aescin on expression of adhesion molecules and migration of neutrophils after middle cerebral artery occlusion in rats显示文摘AIM: To investigate the effects of sodium β-aescin on neutrophil migration and expression of adhesion molecules (ICAM-1 and E-selectin) after middle cerebral artery occlusion (MCAO) in rats. METHODS: Rats were pretreated with sodium β-aescin for 7 d and then subjected to cerebral ischemia/reperfusion (I/R) injury induced by an MCAO. After a 2-h ischemia and a 24-h reperfusion, the infarct volume and neurological deficit were determined by the method of TTC staining and the Longa’s score. The effect of sodium β-aescin on the migration of neutro- phils was evaluated by measuring the activity of myeloperoxidase (MPO) enzyme. The expressions of adhesion molecules were determined by immunohistochemistry and Western blot. RESULTS: Sodium β-aescin significantly reduced the cerebral infarct volume and ameliorated the neurological deficit (P<0.05 or P<0.01). The MPO activity and the expressions of ICAM-1 and E-selectin in the vehicle-treated rats were increased significantly (P<0.01) after cerebral I/R. After treatment with sodium β-aescin, the enzymatic activity of MPO and the expressions of these adhesion molecules were significantly reduced compared with the vehicle-treated group (P<0.05 or P<0.01). CONCLUSION: Sodium β-aescin can attenuate brain injury, down-regulate the protein expressions of ICAM-1 and E-selectin, and reduce the migration of neutrophils after cerebral I/R. | Xia-minHU YanZHANG Fan-dianZENG | 2004 | Acta Pharmacologica Sinica2004，25，7： | 14 |
| 6 | Advances in direct production of value-added chemicals via syngas conversion显示文摘Syngas conversion to fuels and chemicals is one of the most challenging subjects in the field of C1 chemistry. It is considered as an attractive alternative non-petroleum-based production route. The direct synthesis of olefins and alcohols as high value-added chemicals from syngas has drawn particular attention due to its process simplicity, low energy consumption and clean utilization of carbon resource, which conforms to the principles of green carbon science. This review describes the recent advances for the direct production of lower olefins and higher alcohols via syngas conversion. Recent progress in the development of new catalyst systems for enhanced catalytic performance is highlighted. We also give recommendations regarding major challenges for further research in syngas conversion to various chemicals. | Yunlei An Tiejun Lin Fei Yu Yanzhang Yang Liangshu Zhong Minghong Wu Yuhan Sun | 2017 | Science China Chemistry2017，60，7： | 8 |
| 7 | Cloning and expression of ornithine decarboxylase gene from human colorectal carcinoma显示文摘AIM: To construct and express ODC recombinant gene for further exploring its potential use in early diagnosis of colorectal carcinoma.METHODS: Total RNA was extracted from colon cancer tissues and amplified by reverse-transcription PCR with two primers, which span the whole coding region of ODC. The synthesized ODC cDNA was cloned into vector pQE-30 at restriction sites BamH I and Sal I which constituted recombinant expression plasmid pQE30-ODC. The sequence of inserted fragment was confirmed by DNA sequencing,the fusion protein including 6His-tag was facilitated for purification by Ni-NTA chromatographic column.RESULTS: ODC expression vector was constructed and confirmed with restriction enzyme digestion and subsequent DNA sequencing. The DNA sequence matching on NCBI Blast showed 99 % affinity. The vector was transformed into E.coli M15 and expressed. The expressed ODC protein was verified with Western blotting.CONCLUSION: The ODC prokaryote expression vector is constructed and thus greatly facilitates to study the role of ODC in colorectal carcinoma. | Hai-YanHu Xian-XiLiu Chun-YingJiang YanZhang Ji-FengBian YiLu ZhaoGeng Shi-LianLiu Chuan-HuaLiu Xiao-MingWang WeiWang | 2003 | World Journal of Gastroenterology2003，9，4： | 7 |
| 8 | Effects of β-aescin on apoptosis induced by transient focal cerebral ischemia in rats显示文摘AIM: To investigate the effects of β-aescin on apoptosis induced by transient focal brain ischemia in rats. METHODS: Rats were pretreated with β-aescin for 7 d and then subjected to brain ischemia/reperfusion (I/R) injury induced by a middle cerebral artery occlusion, After 2 h ischemia and 24 h reperfusion, Hematoxylin-Eosin (HE) staining, in situ end-labeling of nuclear DNA fragmentation (TUNEL) were employed to determine the level of apoptosis, The expressions of caspase-3 and Bcl-2 in the cortex were determined by immunohistochemistry and Western blot. The release of cytochrome c was analyzed by Western blot. RESULTS: The increased numbers of HE- and TUNEL-positive staining cells were significantly observed at 24 h after reperfusion, The immunoreactivity was inhibited by β-aescin (30, 60 mg/kg) (P<0.01 or P<0.05 vs vehicle-treated), After cerebral I/R, cytochrome c was released into the cytosol and caspase-3 was activated, whereas Bcl-2 expression was inhibited. β-Aescin (30, 60 mg/kg) markedly inhibited the expression of caspase-3 and the release of cytochrome c, and up-regulated the expression of Bcl-2 (P<0.05, P<0.01 vs vehicle-treated). CONCLUSION: β-Aescin could potently inhibit caspase-3 activation and the release of cytochrome c, increasing the expression of Bcl-2 after cerebral I/R in rats, These findings on the inhibitory effects of β-aescin on brain ischemic injury-induced apoptosis might have important theoretical basis for the treatment on ischemic cerebrovascular diseases. | Xia-minHU YanZHANG Fan-dianZENG | 2004 | Acta Pharmacologica Sinica2004，25，10： | 6 |
| 9 | Nonlinear system PID-type multi-step predictive control显示文摘A compound neural network was constructed during the process of identification and multi-step prediction. Under the PlD-type long-range predictive cost function, the control signal was calculated based on gradient algorithm. The nonlinear controller' s structure was similar to the conventional PID controller. The parameters of this controller were tuned by using a local recurrent neural network on-line. The controller has a better effect than the conventional PID controller. Simulation study shows the effectiveness and good performance. | YanZHANG ZengqiangCHEN ZhuzhiYUAN | 2004 | 控制理论与应用(英文版)2004，2，2： | 6 |
| 10 | GGDEF and EAL proteins play different roles in the control of Sinorhizobium melUoti growth, motility, exopolysaccharide production, and competitive nodulation on host alfalfa显示文摘一个新细菌的第二等的送信人,二度 --(3,5 ) 周期的二聚的 guanosine monophosphate (c-di-GMP ) ,通常被综合或由包含的蛋白质分解了 GGDEF 或 glutamatealanineleucine (EAL ) 领域。因为 c-di-GMP 和他们的基因的 cyclase 或 phosphodiesterase 根据已知的 genomic DNA 序列在几乎所有细菌之中是分布式的,他们经常行动。然而, GGDEF 和 EAL 基因的系统的鉴定在 rhizobia 仍然保持不清楚,与兼容的荚交往到的土壤细菌形成修理氮的小瘤。在这研究, 19 通常认为的 GGDEF 和 EAL 基因在模型根瘤菌被识别, Sinorhizobium meliloti,由生物信息的分析(编码 5 GGDEF 蛋白质和 EAL 双域蛋白质) 。14 基因的空异种通过系统的 plasmid 插入被构造。所有 14 基因异种在最小的中等、有缺点的活动性显示出缺乏的生长,并且 11 基因异种生产了更多的 exopolysaccharide 并且在主人工厂上显示了更少的竞争生节,苜蓿。我们的结果建议 GGDEF 和 EAL 蛋白质可以在 S 的控制起不同作用。meliloti 生理学,尽管他们包含保存催化(GGDEF 或 EAL ) 领域。我们发现也暗示了那 c-di-GMP 可以在在这根瘤菌和它的主人之间的相互作用起一个重要作用种建立有效共生。 | Yiwen Wang Ji Xu Aimin Chen Yanzhang Wang Jiabi Zhu Guanqiao Yu Ling Xu Li Luo | 2010 | Acta Biochimica et Biophysica Sinica2010，42，6： | 5 |
| 11 | Leaf nitrogen and phosphorus stoichiometry across 753 terrestrial plant species in China显示文摘 | WenxuanHan JingyunFang DaliGuo YanZhang | 2005 | New Phytologist2005，，2： | 5 |
| 12 | Thrombospondin I activates the macrophage Toll-like receptor 4 pathway显示文摘 | Yanzhang Li Xinyu Qi Xiaopeng Tong Shuxia Wang | 2013 | Cellular & Molecular Immunology2013，10，6： | 4 |
| 13 | Human endostatin gene transfer,either naked or with liposome,has the same inhibitory effect on growth of mouse liver tumor cells in vivo显示文摘AIM: To explore a safe and efficient strategy of tumortherapy using anti-angiogenetic agents.METHODS: Endostatin gene with a signal sequence ofhuman IgG y chain was amplified by PCR and cloned intopVAXl plasmid which was the only vector authorized byFDA in clinical trial to construct a recombinant plasmidnamed as pVAX-sEN. The recombinant plasmid wasdetected with EcoRI/KpnI and DNA sequencing. BALB/c micebearing hepatocarcinoma cell line H22 were treated withnaked pVAX-sEN or liposome-DNA complex in which thedose of DNA and the ratio of DNA and liposome weredifferent from each other. To compare the efficiency ofgene transfection, expression of endostatin at the treatedtumor site was assayed with ELISA. To investigate the effectof pVAXI-sEN on hepatocellular carcinoma, pVAX-sENeither naked or in liposome-DNA complex was injectedinto BALB/c mice bearing H22, then the diameter of tumorswas measured, microvessel density was detected byimmunohistochemistry, endostatin expression in vivo wasassayed at different time points.RESULTS: DNA sequencing showed the endostatin genewith the signal peptide was correctly cloned. In situ geneexpression assay indicated that both the ratio of DNAand liposome and the dose of DNA could affect the genetransfection efficiency. Interestingly, naked pVAX-sEN hada similar in situ endostatin expression to pVAX-sEN withliposome. Animal experiments showed that pVAX-sENtogether with pVAX-sEN-liposome complex could efficientlysuppress the growth of mouse hepatoma cells.CONCLUSION: Naked endostatin plasmid intratumoralinjection can get a similar gene transfection efficiency toliposome-DNA complex when used in situ. | Chun-HongMa YanZhang Xiao-YanWang Li-FenGao HuaLiu ChunGuo Su-XiaLiu Ying-Lincao Li-NingZhang Wen-ShengSun | 2004 | World Journal of Gastroenterology2004，10，19： | 4 |
| 14 | An overview on nonlinear porous flow in low permeability porous media显示文摘This paper gives an overview on nonlinear porous flow in low permeability porous media, reveals the microscopic mechanisms of flows, and clarifies properties of porous flow fluids. It shows that, deviating from Darcy's linear law, the porous flow characteristics obey a nonlinear law in a low-permeability porous medium, and the viscosity of the porous flow fluid and the permeability values of water and oil are not constants. Based on these characters, a new porous flow model, which can better describe low permeability reservoir, is established. This model can describe various patterns of porous flow, as Darcy's linear law does. All the parameters involved in the model, having definite physical meanings, can be obtained directly from the experiments. | Yanzhang Huang Zhengming Yang Ying He Xuewu Wang | 2013 | Theoretical & Applied Mechanics Letters2013，3，2： | 3 |
| 15 | FDI spillovers in an emerging market: the role of foreign firms’ country origin diversity and domestic firms’ absorptive capacity显示文摘 | YanZhang HaiyangLi YuLi Li‐AnZhou | 2010 | Strat Mgmt J2010，，9： | 3 |
| 16 | A novel method for preparation of tissue microarray显示文摘AIM:To improve the technique of tissue microarray (tissue chip).METHODS: A new tissue microarraying method was invented with a common microscope installed with a special holing needle, a sampling needle, and a special box fixing paraffin blocks on the microscope slide carrier. With the movement of microscope tube and objective stage on vertical and cross dimensions respectively, the holing procedure on the recipient paraffin blocks and sampling procedure of core tissue biopsies taken from the donor blocks were performed with the refitted microscope on the same platform.The precise observation and localization of representative regions in the donor blocks were also performed with the microscope equipped with a stereoscope.RESULTS: Highly-qualified tissue chips of colorectal tumors were produced by a new method, which simplified the conventional microarraying procedure, and was more convenient and accurate than that employing the existing tissue microarraying instruments.CONCLUSION:Using the refitted common microscope to produce tissue microarray is a simple, reliable, cost-effective and well-applicable technique. | Han-LeiDan Ya-LiZhang YanZhang Ya-DongWang Zuo-ShengLai Yu-JieYang Hai-HongCui Yan-TingJian JianGeng Yan-QingDing Chun-HaiGuo Dian-YuanZhou | 2004 | World Journal of Gastroenterology2004，10，4： | 3 |
| 17 | Risk prediction models for lung cancer:Perspectives and dissemination显示文摘Objective: The objective was to systematically assess lung cancer risk prediction models by critical evaluation of methodology, transparency and validation in order to provide a direction for future model development.Methods: Electronic searches(including PubMed, EMbase, the Cochrane Library, Web of Science, the China National Knowledge Infrastructure, Wanfang, the Chinese BioMedical Literature Database, and other official cancer websites) were completed with English and Chinese databases until April 30 th, 2018. Main reported sources were input data, assumptions and sensitivity analysis. Model validation was based on statements in the publications regarding internal validation, external validation and/or cross-validation.Results: Twenty-two studies(containing 11 multiple-use and 11 single-use models) were included. Original models were developed between 2003 and 2016. Most of these were from the United States. Multivariate logistic regression was widely used to identify a model. The minimum area under the curve for each model was 0.57 and the largest was 0.87. The smallest C statistic was 0.59 and the largest 0.85. Six studies were validated by external validation and three were cross-validated. In total, 2 models had a high risk of bias, 6 models reported the most used variables were age and smoking duration, and 5 models included family history of lung cancer.Conclusions: The prediction accuracy of the models was high overall, indicating that it is feasible to use models for high-risk population prediction. However, the process of model development and reporting is not optimal with a high risk of bias. This risk affects prediction accuracy, influencing the promotion and further development of the model. In view of this, model developers need to be more attentive to bias risk control and validity verification in the development of models. | Wei Tang Qin Peng Yanzhang Lyu Xiaoshuang Feng Xin Li Luopei Wei Ni Li Hongda Chen Wanqing Chen Min Dai Ning Wu Jiang Li Yao Huang | 2019 | Chinese Journal of Cancer Research2019，31，2： | 2 |
| 18 | Improvement of tribological behavior of a Ti-Al-V alloy by nitrogen ion implantation显示文摘The tribological properties especially wear and hardness of a Ti-Al-V alloy with nitrogen implantation (energy 60 keV) were investigated. The implantation was carried out at fluences range from 1×1016 to 4×1017 ions/cm2. Glancing angle X-ray diffraction (GAXRD) and X-ray photoelectron spectroscopy (XPS) analyses were performed to obtain surface characterization of the implanted sample. The unimplanted and implanted samples were also annealed at 600 ℃ in order to understand the influence of annealing on the tribological properties of Ti-Al-V. The hardness shows significant improvement at the higher fluence. After annealing at 600 ℃, the friction coefficient exhibits a relative decrease for the nitrogen-implanted samples. In addition, the wear rates of the implanted samples exhibits a great decrease after annealing at 600 ℃. Nature of the surface and reason for the variation and improvement in wear resistance were discussed in detail. | LIU Yanzhang ZU Xiaotao QIU Shaoyu HUANG Xinquan | 2006 | Rare Metals2006，25，z1： | 2 |
| 19 | Cloning, identification, and expression analysis at the stage of gonadal sex differentiation of chicken miR-363 and 363显示文摘miRNAs (microRNAs ) 是小的,功能的、非编码的 RNA 并且被证明了在从房间区别到有机体开发的多样的生物过程的规定含有。以达到在鸡胚胎上探索 miRNAs 的角色性决心和 gonadal 区别,我们克隆并且识别茎环先锋结构( GenBank 同意没有 GU597370 )鸡肉, miR-363 和363*由在 E3.56.5 d 的阶段在鸡胚胎学习他们的时间、空间的表示模式列在后面(胚胎的天 3.56.5 )由半量的 RT-PCR 和愿望(在 situ 杂交整个山)在这研究。结果显示出那 miR-363 * 根据 miRNAs 的结构的特征在 miR-363 和 363* 的鸡染色体,和 flanking 顺序在未知片断的克隆的顺序定位了先锋。显著地微分的表示(P < 0.05 ) 在雌、雄的鸡肉之间的 gga-miR-363,胚胎的性腺被发现在 E4.5 和 6.5 d,而是 gga-miR-363 的微分表示 * 从 E3.5,到 6.5,在两性之间的 d 达不到重要水平。那个表达式 gga-miR-363 表明的显示的愿望的结果主要在 E6.5 出现在手足芽,脊索,外胚层,在 E4.5 d 鸡胚胎的大脑,和尿生殖的系统(UGS ) d,和 E6.5 d 的表达式水平在男性比那在女性是更高的。gga-miR-363 将在 gonadal 发展和 gga-miR-363 包含,这能被推测 * 可能在鸡胚胎开发的早阶段期间有短暂规章的功能。 | Pan Huang Yanzhang Gong Xiuli Peng ShijunLi Yu Yang Yanping Feng | 2010 | Acta Biochimica et Biophysica Sinica2010，42，8： | 2 |
| 20 | Once an outsider, always an outsider? CEO origin, strategic change, and firm performance显示文摘 | YanZhang NandiniRajagopalan | 2010 | Strat Mgmt J2010，，3： | 2 |
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