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| 1 | Anti-tumor effect of pEgr-interferon-γ-endostatin gene-radiotherapy in mice bearing Lewis lung carcinoma and its mechanism显示文摘Background Gene-radiotherapy, the combination of gene therapy and radiation therapy, is a new paradigm for cancer treatment. To enhance anti-tumor effect of gene-radiotherapy, in this study we construct a radiation-inducible dual-gene co-expression vector pEgr-interferon(IFN)-γ- endostatin and studied the anti-tumor effect of pEgr-IFN-γ-endostatin gene-radiotherapy in mice bearing Lewis lung carcinoma and its mechanism.Methods Gene recombinant technique was used to construct dual-gene co-expression plasmid pEgr-IFN-γ-endostatin, and single-gene expression plasmid pEgr-IFN-γ and pEgr-endostatin. The plasmids packed by liposome were injected locally into the tumors of the mice, and the tumors were irradiated with 5 Gy X-ray 36 hours later. The tumor growth rate at different time and mean survival period of the mice were observed. Cytotoxic activity of splenic cytotoxic T-lymphocyte (CTL), natural killer (NK) cell and tumor necrosis factor (TNF)-α secretion activity of peritoneal macrophages of the mice in various groups were evaluated 15 days after irradiation. The intratumor micro-vessel density was evaluated by immunohistochemical staining 10 days after irradiation.Results The tumor growth rate of the mice in dual-gene-radiotherapy group was significantly lower than those in control group, 5 Gy group and single-gene-radiotherapy group at different time after gene-radiotherapy, and the mean survival period of which was longer. Cytotoxic activity of splenic CTL, NK and TNF-α secretion activity of peritoneal macrophages of the mice in dual-gene-radiotherapy group were significantly higher than those in control group, 5 Gy X-ray irradiation group and pEgr-endostatin gene-radiotherapy group 15 days after irradiation. The intratumor micro-vessel density of the mice in dual-gene-radiotherapy group was significantly lower than those in control group, 5 Gy X-ray irradiation group and pEgr-IFN-γgene-radiotherapy group. Conclusion The anti-tumor effect of dual-gene-radiotherapy was significantly better than that of single-gene-radiotherapy by combining the enhancement of anti-tumor immunologic function induced by IFN-γ with the anti-angiogenesis function of endostatin. | YANGWei LIXiu-yi | 2005 | Chinese Medical Journal2005，，4： | 20 |
| 2 | Biphasic insulin aspart 30 improved glycemic control in Chinese patients with type 2 diabetes poorly controlled on oral glucose-lowering drugs: a subgroup analysis of the A1chieve study显示文摘 | Chen Liming Xing Xiaoyan Lei Minxiang Liu Jie Shi Yongquan Li Pengqiu Qin Guijun Li Chengjiang Li Yukun Wang Qing Gao Tianshu Hu Ling Wang Yangwei Yang Wenying | 2014 | Chinese Medical Journal2014，，2： | 19 |
| 3 | Design and Experiment on a Biomimetic Robotic Fish Inspired by Freshwater Stingray显示文摘 | Yangwei Wang Jinbo Tan Dongbiao Zhao | 2015 | Journal of Bionic Engineering2015，12，2： | 16 |
| 4 | Numerical study of corner separation in a linear compressor cascade using various turbulence models显示文摘Three-dimensional corner separation is a common phenomenon that significantly affects compressor performance.Turbulence model is still a weakness for RANS method on predicting corner separation flow accurately.In the present study,numerical study of corner separation in a linear highly loaded prescribed velocity distribution(PVD) compressor cascade has been investigated using seven frequently used turbulence models.The seven turbulence models include Spalart–Allmaras model,standard k–e model,realizable k–e model,standard k–x model,shear stress transport k–x model,v^2–f model and Reynolds stress model.The results of these turbulence models have been compared and analyzed in detail with available experimental data.It is found the standard k–e model,realizable k–e model,v^2–f model and Reynolds stress model can provide reasonable results for predicting three dimensional corner separation in the compressor cascade.The Spalart–Allmaras model,standard k–x model and shear stress transport k–x model overestimate corner separation region at incidence of 0°.The turbulence characteristics are discussed and turbulence anisotropy is observed to be stronger in the corner separating region. | Liu Yangwei Yan Hao Liu Yingjie Lu Lipeng Li Qiushi | 2016 | Chinese Journal of Aeronautics2016，29，3： | 11 |
| 5 | ^(7)Be and^(210)Pb radioactivity and implications on sources of surface ozone at Mt.Waliguan显示文摘Beryllium-7 (7Be) and lead-210 (210Pb) radioac- tivity in aerosols collected, from October 2002 to January 2004 at Mt. Waliguan, by the Global Atmospheric Watch (GAW) Station, Qinghai Province is presented. The data were analyzed together with simultaneously measured surface ozone concentrations. We found that short time variations of 7Be and 210Pb were linked to alternations of synoptic weather around the Mt. Waliguan region. 210Pb showed the lowest concentration in summer while Be showed no obvious sea- 7 sonal changes. Relatively high Be and 7 210 Pb radioactivity was observed at Mt. Waliguan when compared with the ob- servations at other mountain sites in other parts of the world. Surface ozone and 7Be showed a consistent seasonalvariation. Surface ozone correlated fairly well with 7Be/210Pb ratio. This suggested that vertical transport from higher altitudes of the atmosphere has predominant effects on the budget of surface ozone at Mt. Waliguan. | ZHENGXiangdong WANGGuojiang TANGJie ZHANGXiaochun YANGWei H.N.Lee WANGChangsheng | 2005 | Chinese Science Bulletin2005，50，2： | 8 |
| 6 | Distribution Characteristics and Metallogenic Regularity of Graphite Deposits in Qinling Orogen,China显示文摘Qinling orogen is one of the five main repository distribution provinces of large scale graphite resources. Graphite occurrence strata are multitudinous including Neo Archaean group to Neopaleozoic. Mineral deposit types are complete consisting of crystal flaky graphite deposit and aphanitic graphite deposit, ore types of the former are main graphite gneiss, graphite schist and graphitized marble, and ore types of the latter are main graphite layer metamorphosised from coal bed and graphitic carbargilite. At present, most graphite deposits with low research degree only implement preliminary investigations of the graphite resource, which has a good prospect. Based on the basic material of dozens of graphite deposits, spots and plays discovered in the Qinling orogen, this paper applied geologic multidisciplinary analysis method to disclose the graphite deposit types of the Qinling orogen, mainly through outdoors geologic survey and specimen examination with analysis of some typical deposits: regional metamorphism crystal graphite deposits and thermo-contact metamorphic aphanitic graphite deposits, with the control of latitudinally trending regional tectonic, graphite deposits of the Qinling orogen distribute sublatitudinally trending three large ore belts: I the Neoarchean–Proterozoic basement crystal graphite enrichment deposits zone along the south margin of North China plate; II the Carboniferous intermountain basin group aphanitic graphite enrichment deposits zone near the Shangdan suture of the West Qinling; III Paleozoic crystal graphite enrichment deposits zone in the Qinling paleomicroplate of the west part of the East Qinling.Conclusions are reached from multiple inquiries: Qinling multi-cycle complex continental collision orogen zone has experienced multiple tectonic framework transformation and polyphase tectonic thermal event from Neo Archean Erathem adjointing multiple graphite tectonic mineralization cycle. In the light of chief control of mineralization and ore types, each of the three large graphite deposits belts of the Qinling orogen has its own genesis: I graphite deposits enriched metallogenetic zone is that regional tectogenetic movement from Neo Archaean caused polyphase metapepsis, which superimposed up and reconstructed Neo Archean Erathem to Proterozoic basement, forming regional metamorphic big flake crystal graphite deposits; II graphite deposits enriched metallogenetic zone is that polyphase regional tectogenetic movement associated with multiple heating caused by multiphase igneous intrusion, which resulted in original coal layers emerging multiple thermo-contact metamorphisms and formed thermo-contact metamorphic aphanitic graphite deposits. III graphite deposits enriched metallogenetic zone is that Palaeozoic cap–rock experienced polyphase metapepsis, forming regional metamorphic finely flake crystal graphite deposits. Comprehensive research of metallogenetic mechanism in graphite deposits and its associated profitable deposits of the Qinling orogen is importance for future exploration. | FENG Yangwei QU Hongjun YANG Chenyi LV Lushi | 2015 | Acta Geologica Sinica(English Edition)2015，89，4： | 7 |
| 7 | Modified k-ω model using kinematic vorticity for corner separation in compressor cascades显示文摘A new method of modifying the conventional k-ω turbulence model for corner separation is proposed in this paper. The production term in the ω equation is modified using kinematic vorticity considering fluid rotation and deformation in complex geometric boundary conditions. The corner separation flow in linear compressor cascades is calculated using the original k-ω model, the modified k-ω model and the Reynolds stress model(RSM). The numerical results of the modified model are compared with the available experimental data, as well as the corresponding results of the original k-ω model and RSM. In terms of accuracy, the modified model, which significantly improves the performance of the original k-ω model for predicting corner separation, is quite competitive with the RSM. However, the modified model, which has considerably lower computational cost, is more robust than the RSM. | LIU YangWei YAN Hao FANG Le LULiPeng LI QiuShi SHAO Liang | 2016 | Science China(Technological Sciences)2016，59，5： | 7 |
| 8 | A combined study of SHRIMP U-Pb dating, trace element and mineral inclusions on high-pressure metamorphic overgrowth zircon in eclogite from Qinglongshan in the Sulu terrane显示文摘Methods recently advanced for discrimination on the genesis of metamorphic zircon, such as analysis of mineral inclusions and trace elements, provide us powerful means to distinguish zircon overgrowth during high-pressure metamorphism. Zircons in ultrahigh-pressure eclogite from Qinglongshan in the Sulu terrane were studied by the SHRIMP U-Pb method in combining with trace element and mineral inclusion analyses. No inherited core was identified in the analyzed zircons by means of cathodoluminescence images. The occurrence of high-pressure metamorphic mineral inclusions in zircon, such as garnet, omphacite, rutile, and the flat HREE pattern in zircon indicate that the zircon formed at high-pressure metamorphic conditions. Therefore, a weighted average U-Pb age of 227.4 ± 3.5 Ma obtained from such a kind of zircon is interpreted to represent the timing of peak metamorphism for the Qinglongshan eclogite. | LiQiuli LiShuguang HOUZhenhui HONGJi'an YANGWei | 2005 | Chinese Science Bulletin2005，50，5： | 6 |
| 9 | Investigation of model development for deterministic correlations associated with impeller-diffuser interactions in centrifugal compressors显示文摘The average-passage equation system(APES)provides a rigorous framework to account for the deterministic unsteady effects by the so-called deterministic correlations(DC),which include both deterministic stress correlations(DCS)and deterministic total enthalpy correlations(DCH).These correlations should be modeled to close the system of equations.In this paper,the distribution of DC in a transonic centrifugal compressor is presented,and its relative importance is revealed.The assumption made by Adamczyk that the pure unsteady fluctuation is significantly smaller than the spatial fluctuation is verified at the impeller-diffuser interface.The decomposition of DCH is also discussed to determine its two different physical mechanisms.Finally,the transport equations in terms of DCS in cylindrical coordinates are derived,and the terms are evaluated to determine the ones that are necessary to model.All these analyses significantly contribute to our model development for DC in centrifugal compressors. | LIU BaoJie ZHANGBo LIU YangWei | 2015 | Science China(Technological Sciences)2015，58，3： | 5 |
| 10 | A Prototype of a Biomimetic Mantle Jet Propeller Inspired by Cuttlefish Actuated by SMA Wires and a Theoretical Model for Its Jet Thrust显示文摘 | Fei Gao Zhenlong Wang Yukui Wang Yangwei Wang Jian Li | 2014 | Journal of Bionic Engineering2014，11，3： | 4 |
| 11 | Transplantation of human limbal cells cultivated on amniotic membrane for reconstruction of rat corneal epithelium after alkaline burn显示文摘Background The transplantation of limbal epithelial cells cultivated on amniotic membrane is a newly developed treatment for limbal stem cell deficiency. The purpose of our study was to investigate the biological characteristics of limbal epithelial cells and evaluate the effect of transplantation of cultivated human limbal epithelial cells on ocular surface reconstruction in limbal stem cell deficiency rat model. Methods Human limbal cells were isolated and cultivated in vitro. Cytokertins 3, 12, and 19 (K3, K12 and K19) and p63 were detected by immunofluorescent staining or RT-PCR. BrdU labelling test was used to identify the slow cycling cells in the cultures. Limbal stem cell deficiency was established in rat cornea by alkali burn. Two weeks after injury, the rats received transplants of human limbal stem cells cultivated on amniotic membrane carrier. The therapeutic effect was evaluated by slit lamp observation, Hemotoxin and Eosin (HE) staining and immunofluorescent staining.Results On day 7 in primary culture, p63 and K19 were strongly expressed by most cells but only a few cells expressed K3. On days 14 and 21, p63 and K19 were still expressed by a majority of cells, but the expressive intensity of p63 decreased in a number of cells, while the proportion of K3 positive cells increased slightly and some cells coexpressed p63 and K3. RT-PCR showed that gene expression of both p63 and K12 were positive in cultivated limbal cells, but in mature superficial epithelial cells, only K12 was detected. BrdU labelling test showed that most cells were labelled with BrdU after 7 days’ labelling and BrdU label retaining cells were observed after chasing for 21 days with BrdU free medium. For in vivo test, slit lamp observation, HE staining and immunofluorescent staining showed that the rats receiving transplant of human limbal stem cells cultivated on amniotic membrane grew reconstructed corneas with intact epithelium, improved transparency and slight or no neovascularization. A majority of epithelial cells of the reconstructed cornea were positive to antihuman nuclear antibody and cells expressing K3 were found mainly in superfacial epithelium.Conclusions Limbal stem cells can be cultivated in vitro: the cells are characterized by high proliferation and slow cycling and identified as p63/K19 positive and K3/K12 negative. During culture, some stem cells can proliferate and differentiate into mature cornea epithelial cells. Amniotic membrane is a suitable carrier for limbal stem cells. Transplantation of human limbal stem cells cultivated on amniotic membrane can functionally reconstruct rat cornea with limbal stem cell deficiency. | SONGE YANGWei CUIZhi-hua DONGYu SUIDong-ming GUANXiao-kang MAYang-ling | 2005 | Chinese Medical Journal2005，，11： | 4 |
| 12 | Control of Corner Separation by Optimized Slot Configuration in a Linear Compressor Cascade显示文摘为控制压气机叶片吸力面与端壁处所形成的三维角区分离,本文在叶根处引入了从压力面到吸力面的槽道来控制角区分离流动。基于利用优化算法对叶根处槽道几何的优化结果,本文通过数值模拟和实验来分析槽道在不同进口攻角下对角区分离的控制效果。本文通过对比原始和开槽叶栅中总压损失、静压升和三维角区分离特征表明叶根处引入的槽道对角区分离有明显的控制效果。 | Jinjing Sun Yangwei Liu Lipeng Lu Xavier OTTAVY | 2016 | 风机技术2016，58，6： | 4 |
| 13 | A Flexible Hingeless Control Surface Inspired by Aquatic Animals显示文摘A flexible hingeless control surface model was proposed for motion control of Underwater Vehicles (UVs),which is inspiredby the flexible bending control surfaces of underwater creatures,such as fish and squid.Computational Fluid Dynamics(CFD) simulation demonstrates that,in comparison with the hinged or rigid control surface,the proposed flexible bendingcontrol surface can suppress the flow separation so as to improve the turning performance.A prototype of the flexible controlsurface was fabricated,in which Shape Memory Alloy (SMA) wires were selected as the actuators.The elastic energy storageand exchange mechanism was incorporated into the actuation of the control surface to improve the efficiency.Thermal analysisof SMA wires was performed to find proper actuating condition.Open-loop bending experiments were carried out.The resultsshow that the proposed control surface can achieve the maximum bending angle of 104°.Moreover,the power and energyconsumption under different pulse conditions were compared. | Zhenlong Wang,Jian Li,Guanrong Hang,Yangwei Wang State Key Laboratory of Robotics and System,Harbin Institute of Technology,Harbin 150001,P.R.China | 2010 | Journal of Bionic Engineering2010，7，4： | 3 |
| 14 | Kinematic Optimization of Bionic Shoulder Driven by Pneumatic Muscle Actuators Based on Particle Swarm Optimization显示文摘A bionic shoulder joint with three degree-of-freedom(DOF)driven by pneumatic muscle actuator is proposed and its corresponding kinematic model is established.The bionic shoulder is optimized by particle swam optimization(PSO)with the fitness standards that the requirements of rotation indexes are met and the fluctuation of motion is kept in the lowest resolution in a pneumatic muscle actuator range.Simulation considering rotation indexes only(first simulation)is compared with the one considering both rotation indexes and motion resolution(second simulation)subsequently.Mounting position of the pneumatic muscle actuators in bionic shoulder is optimized after initializing the same condition in simulations.Results show that the fluctuations of parameters are consistent,and the parameters of the first simulation have good convergence than those of the second one.With the increase of stretch rate of the pneumatic muscle actuator,the needed length of fixed link in the center of static platform decreases in optimization. | Liu Kai Ge Zhishang Xu Jiaqi Gu Baotong Wang Yangwei Zhao Dongbiao | 2016 | Transactions of Nanjing University of Aeronautics and Astronautics2016，33，3： | 3 |
| 15 | Phenotypic and genetic parameter estimation of juvenile growth and bottom color traits in half-smooth tongue sole,Cynoglossus semilaevis显示文摘Half-smooth tongue sole(Cynoglossus semilaevis) is a commercially important fish species. There were no reports about accurate genetic parameters of growth traits in C. semilaevis, despite efforts aimed at the culture of this species. Therefore, the main goal of this study was to estimate the parameters for the early growth traits(total length, body depth and body weight) of C. semilaevis based on the data obtained from 80 families. The trait of fish with pure white bottom or not(named as the trait of bottom color) was also analyzed. All the heritabilities estimated for the traits were medium, which varied from 0.210 to 0.362. Genetic correlations among growth traits were highly positive, varying from 0.913 to 0.959, indicating that selection of one of the three traits would result in correlated increase in the others. Also, genetic correlations between bottom color and growth traits were positive and varying from 0.241 to 0.353, suggesting that selection for the bottom color can be utilized to enhance the selection of growth traits. In addition, fish from the top performing 16 full-sib families were selected as broodstock, based on the above analysis and the comparison of family breeding value. This research provides an important basic material to implement selective breeding in C. semilaevis. | LIU Feng | 2016 | Acta Oceanologica Sinica2016，35，10： | 2 |
| 16 | Postharvest Biological Control of Apple Anthracnose by Bacillus subtilis BS80-6,A Mutant Strain through Ion Implantation显示文摘The original Bacillus subtilis was isolated from the soil of an apple orchard,and B. subtilis strain BS80-6,selected from Bacillus subtilis by ion implantation,was used as an antagonist for postharvest biological control against apple anthracnose( colletotrichum gloeosporioides). The mechanisms of action and efficacy of Bacillus subtilis strain BS80-6 against apple anthracnose caused by colletotrichum gloeosporioides were studied in vitro and on apples in controlled and semi-commercial conditions. An application of cell suspension( 108 cells per mL) of the antagonist in artificial wounds of apples reduced growth of C. gloeosporioides after storage at different temperatures. The inhibitory actions of cell culture to hyphal growth and to spores germination were 86. 6% and 98. 65%,respectively,and the control efficacy of cell culture against the disease was 60. 34% at room temperature. The results showed that BS80-6 was more effective against apple anthracnose.The cell culture of BS80-6 received better control efficacy against apple anthracnose than culture filtrates and autoclaved cell. All treatments significantly inhibited the disease both in vitro and in vivo compared with control. Fruits treated with cell culture and stored at 10 ℃ had lower infection rate,more delayed formation of acervwlus and smaller lesion diameter than those stored at 20 ℃. There was better control efficacy in fruits inoculated with spores after application of BS80-6. The mechanisms of BS80-6 activity appeared to involve production of an antifungal substance,deformation of hyphal cell and disintegration of cell wall. Besides,BS80-6 could improve the activity of major defense enzymes of apple,such as peroxidase( POD) and polyphenol oxidase( PPO). | Tan Genjia Fei Dan Shi Yangwei Liu Shufang Li Li Li Zengzhi | 2014 | Plant Diseases and Pests2014，5，3： | 2 |
| 17 | Short-DNA Specific Blocker PCR for Efficient and Simple Enrichment of Cell Free Fetal DNAs with Short Lengths显示文摘As an inn ate characteristic,DNA length has attracted more and more atte ntion.The length of some import ant biomarkers such as ctDNA(Circulating tumor DNA)and cffDNA(Cell free fetal DNA)is shorter than that of cell-free DNA.Researchers have utilized the differe nee in length to in crease the abunda nee of ctDNA or cffDNA in total cell-free DNA to overcome the difficulties in detecti on due to their low abundance. | Yangwei Liao Xiaofeng Tang Zhihao Ming Lida Ren Wei Zhang Xianjin Xiao | 2021 | Chinese Journal of Chemistry2021，39，8： | 2 |
| 18 | Synthesis and self-assembly of poly(ethylene glycol)-block-poly(N-3-(methylthio)propyl glycine)and their oxidation-sensitive polymersomes显示文摘Amphiphilic block copolymers poly(ethylene glycol)-block-poly(N-3-(methylthio)propyl glycine)(PEGb-PMeSPG)were synthesized via ring-opening polymerization of N-3-(methylthio)propyl glycine Nthiocarboxyanhydride(MeSPG-NTA)initiated by amino-terminated PEG.The self-assemblies of three PEG-b-PMeSPG copolymers with different PMeSPG block lengths were first prepared by nanoprecipitation method using THF and DMF,respectively,as the organic solvent,and their morphologies were studied by Cryo-EM and DLS.To prepare polymersomes loaded with glucose oxidase(GOx),double emulsion method followed by extrusion treatment was employed.The oxidation-responsive disruption of polymersomes was achieved upon the introduction of glucose because of the oxidants generated insitu by GOx/glucose. | Yangwei Deng Hui Chen Xinfeng Tao Sylvain Trepout Jun Ling Min-Hui Li | 2020 | Chinese Chemical Letters2020，31，7： | 2 |
| 19 | Rotation Control of a 3-DOF Parallel Mechanism Driven by Pneumatic Muscle Actuators显示文摘The pneumatic muscle actuator(PMA)has many advantages,such as good flexibility,high power/weight ratio,but its nonlinearity makes it difficult to build a static mathematical model with high precision.A new method is proposed to establish the model of PMA.The concept of hybrid elastic modulus which is related to the static characteristic of PMA is put forward,and the energy conservation law is used to achieve the expression of the hybrid elastic modulus,which can be fitted out based on experimental data,and the model of PMA can be derived from this expression.At the same time,a 3-DOF parallel mechanism(a new bionic shoulder joint)driven by five PMAs is designed.This bionic shoulder joint adopts the structure of two antagonistic PMAs actualizing a rotation control and three PMAs controlling another two rotations to get better rotation characteristics.The kinematic and dynamic characteristics of the mechanism are analyzed and a new static model of PMA is used to control it.Experimental results demonstrate the effectiveness of this new static model. | LIU Kai CHEN Yining WU Yang XU Jiaqi WANG Yangwei GE Zhishang | 2019 | Transactions of Nanjing University of Aeronautics and Astronautics2019，36，2： | 2 |
| 20 | A Modeling Investigation on the Electrochemical Behavior of Porous Mixed Conducting Cathodes for Solid Oxiide Fuel Cells 显示文摘 | Zeng Yangwei Tian Changan Bao Limei | 2003 | Journal of Power Sources2003，121，： | 1 |
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