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| 1 | Crystal structures of D-psicose 3-epimerase from Clostridium cellulolyticum H10 and its complex with ketohexose sugars显示文摘D-Psicose 3-epimerase(DPEase)is demonstrated to be useful in the bioproduction of D-psicose,a rare hexose sugar,from D-fructose,found plenty in nature.Clostridium cellulolyticum H10 has recently been identified as a DPEase that can epimerize D-fructose to yield D-psicose with a much higher conversion rate when compared with the conventionally used DTEase.In this study,the crystal structure of the C.cellulolyticum DPEase was determined.The enzyme assembles into a tetramer and each subunit shows a(β/α)8 TIM barrel fold with a Mn2+metal ion in the active site.Additional crystal structures of the enzyme in complex with substrates/products(D-psicose,D-fructose,D-tagatose and D-sorbose)were also determined.From the complex structures of C.cellulolyticum DPEase with D-psicose and D-fructose,the enzyme has much more interactions with D-psicose than D-fructose by forming more hydrogen bonds between the substrate and the active site residues.Accordingly,based on these ketohexosebound complex structures,a C3-O3 proton-exchange mechanism for the conversion between D-psicose and D-fructose is proposed here.These results provide a clear idea for the deprotonation/protonation roles of E150 and E244 in catalysis. | Hsiu-Chien Chan Yueming Zhu Yumei Hu Tzu-Ping Ko Chun-Hsiang Huang Feifei Ren Chun-Chi Chen Yanhe Ma Rey-Ting Guo Yuanxia Sun | 2012 | Protein & Cell2012，3，2： | 6 |
| 2 | Precise temporal regulation of Dux is important for embryo development显示文摘Dear Editor,Zygotic genome activation(ZGA)following fertilization is accomplished through a process termed the maternal-to-zygotic transition,during which the maternal RNAs and proteins are degraded and zygotic genome is transcriptionally activated.In mice,minor ZGA occurs from S phase of the zygote to G1 phase of the two-cell(2C)embryo,while major ZGA takes place during the middle-to-late 2C stage with a burst of transcription of totipotent cleavage stage-specific genes and retrotransposons. | Mingyue Guo Yanping Zhang Jianfeng Zhou Yan Bi Junqin Xu Ce Xu Xiaochen Kou Yanhong Zhao Yanhe Li Zhifen Tu Kuisheng Liu Jiaming Lin Peng Yang Shaorong Gao Yixuan Wang | 2019 | Cell Research2019，29，11： | 5 |
| 3 | Damage Identification of General Overhead Travelling Crane Structure Based on Model Updating by Sensitivity显示文摘A model based damage identification was proposed by facilitating parameter sensitivity analysis and applied to a general overhead travelling crane.As updating reference data,experimental modal frequency was obtained by operational modal analysis(OMA)under ambient excitation.One dimensional damage function was defined to identify the damage by bending stiffness.The results showed that the model updating method could locate the damage and quantitatively describe the structure.The average error of eigenvalues between updated model analysis and the experimental results was less than 4% which proved the accuracy reliable.The comparison of finite element analysis and the test results of the deflection under the capacity load further verified the feasibility of this method. | Qing Guangwei Yue Lin Guo Qingtao Tao Yanhe Hu Jingbo | 2017 | Transactions of Nanjing University of Aeronautics and Astronautics2017，34，3： | 1 |
| 4 | MiR-18a regulates expression of the pancreatic transcription factor Ptf1a in pancreatic progenitor and acinar cells显示文摘 | Yankun Yang Lei Ding Yang An Zhen-wu Zhang Yanhe Lang Sheng Tai Fangqi Guo Chun-Bo Teng | 2012 | FEBS Letters2012，，4： | 1 |
| 5 | Cancer survival in C hina, 2003–2005: A population‐based study显示文摘 | Hongmei Zeng Rongshou Zheng Yuming Guo Siwei Zhang Xiaonong Zou Ning Wang Limei Zhang Jingao Tang Jianguo Chen Kuangrong Wei Suqin Huang Jian Wang Liang Yu Deli Zhao Guohui Song Jianshun Chen Yongzhou Shen Xiaoping Yang Xiaoping Gu Feng Jin Qilong Li Yanh | 2015 | Int. J. Cancer2015，，8： | 1 |
| 6 | Cancer survival in C hina, 2003–2005: A population‐based study显示文摘 | Hongmei Zeng Rongshou Zheng Yuming Guo Siwei Zhang Xiaonong Zou Ning Wang Limei Zhang Jingao Tang Jianguo Chen Kuangrong Wei Suqin Huang Jian Wang Liang Yu Deli Zhao Guohui Song Jianshun Chen Yongzhou Shen Xiaoping Yang Xiaoping Gu Feng Jin Qilong Li Yanh | 2015 | Cancer2015，，8： | 1 |
| 7 | Effect of incubation conditions and possible intestinal nutrients on cis- 9 , trans- 11 conjugated linoleic acid production by Lactobacillus acidophilus F0221显示文摘 | Jingyan Li Lanwei Zhang Xue Han Huaxi Yi Chunfeng Guo Yingchun Zhang Ming Du Xue Luo Yanhe Zhang Yujuan Shan | 2013 | International Dairy Journal2013，，2： | 1 |
| 8 | Thermodynamic studies on supramolecular interactions of metal ions with nucleotides/tripods ligands 显示文摘 | Yanhe Guo Ge Qinchun Lin Hai | 2002 | Polyhedron2002，21，8： | 1 |
| 9 | Bilineage embryo-like structure from EPS cells can produce live mice with tetraploid trophectoderm显示文摘Self-organized blastoids from extended pluripotent stem(EPs)cells possess enormous potential for investigating postimplantation embryo development and related diseases.However,the limited ability of postimplantation development of Eps-blastoids hinders its further application.In this study,single-cell transcriptomic analysis indicated that the“trophectoderm(TE)-like structure”of EPSblastoids was primarily composed of primitive endoderm(PrE)-related cells instead of TE-related cells.We further identified PrE-like cells in EPS cell culture that contribute to the blastoid formation with TE-like structure.Inhibition of PrE cell differentiation by inhibiting MEK signaling or knockout of Gata6 in EPS cells markedly suppressed EPS-blastoid formation.Furthermore,we demonstrated that blastocyst-like structures reconstituted by combining the EPs-derived bilineage embryo-like structure(BLEs)with either tetraploid embryos or tetraploid TE cells could implant normally and develop into live fetuses.In summary,our study reveals that TE improvement is critical for constructing a functional embryo using stem cells in vitro. | Kuisheng Liu Xiaocui Xu Dandan Bai Yanhe Li Yalin Zhang Yanping Jia Mingyue Guo Xiaoxiao Han Yingdong Liu Yifan Sheng Xiaochen Kou Yanhong Zhao jiqing Yin Sheng Liu jiayu Chen Hong Wang Yixuan Wang Wenqiang Liu Shaorong Gao | 2023 | Protein & Cell2023，14，4： | 0 |
| 10 | Genome transfer for the prevention of female infertility caused by maternal gene mutation显示文摘Poor oocyte quality is associated with early embryo developmental arrest and infertility.Maternal gene plays crucial roles in the regulation of oocyte maturation,and its mutation is a common cause of female infertility.However,how to improve oocyte quality and develop effective therapy for maternal gene mutation remains elusive.Here,we use Zar1 as an example to assess the feasibility of genome transfer to cure maternal gene mutationecaused female infertility.We first discover that cytoplasmic deficiency primarily leads to Zar1-null embryo developmental arrest by disturbing maternal transcript degradation and minor zygotic genome activation(ZGA)during the maternal-zygotic transition.We next perform genome transfer at the oocyte(spindle transfer or polar body transfer)and zygote(early pronuclear transfer or late pronuclear transfer)stages to validate the feasibility of preventing Zar1 mutationecaused infertility.We finally demonstrate that genome transfer either at the oocyte or at the early pronuclear stage can support normal preimplantation embryo development and produce live offspring.Moreover,those pups grow to adulthood and show normal fertility.Therefore,our findings provide an effective basis of therapies for the treatment of female infertility caused by maternal gene mutation. | Dandan Bai Jin Sun Yanping Jia Jiqing Yin Yalin Zhang Yanhe Li Rui Gao Xiling Du Kunming Li Jiaming Lin Zhifen Tu Yu Wang Jiaping Pan Shanshan Liang Yi Guo Jingling Ruan Xiaochen Kou Yanhong Zhao Hong Wang Cizhong Jiang Fengchao Wang Xiaoming Teng Wenqiang Liu Shaorong Gao | 2020 | Journal of Genetics and Genomics2020，47，6： | 0 |
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