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| 1 | Origin area and migration route: Chloroplast DNA diversity in the arctic-alpine plant Koenigia islandica显示文摘The Hengduan Mountains(henceforth H-D Mountains) on the Tibet Plateau are a distribution and diversity center for many alpine genera. We examine patterns of genetic variation in an arctic-alpine plant to evaluate the possibility that the H-D Mountains constitute the area of origin of the species as well as to uncover postglacial migration routes. 220 individuals of the arctic-alpine plant Koenigia islandica were sampled from 26 populations distributed in western China and northern Finland. DNA haplotypes were identified using restriction site analysis of two chloroplast DNA intergene spacer regions, atpB-rbcL and trnL-trnF. We examined the geographical distribution of haplotype diversity in relation to latitude, and also compared various indices of diversity in putatively glaciated and unglaciated regions. Patterns of migration were inferred using nested clade analysis. A total of 25 haplotypes were detected. High haplotype diversity was found in the H-D Mountains. H3 and its radiated haplotypes were distributed in the Himalayas. Two haplotypes were fixed concurrently in the H-D Mountains and northern Finland. High genetic diversity of K. islandica and high species diversity of K. islandica are expected in the origin area. Our observations suggest that the H-D Mountains are not only the place of origin of K. islandica, but also the refugia for K. islandica on the Tibet Plateau. What is more, the migration route for the arctic-alpine plant K. islandica must have originated in the region defined by the H-D Mountains in western China extending northward to the Arctic circumpolar, and moved westward along the Himalayas, then northward across the Altay Mountains and the Central Siberian Plateau at different time periods. | LONG Cong MIN YunJiang ZHAO XiuXia YANY ChunLei SUN Hang Lü HouYuan TANG LingYu ZHOU ZhongZe | 2014 | Science China Earth Sciences2014，57，8： | 2 |
| 2 | Body size, recrea- tional physical activity, and B-cell non-Hodgkin lymphoma risk among women in the California teachers study显示文摘 | Yani L Prescott J Sullivan-Halley J | 2009 | Am J Epide- miol2009，17，： | 1 |
| 3 | The purification and sequence ofa temperature-sensitive tryptophan tRNA 显示文摘 | isenberg SP Soil L Yanis M | 1979 | J Biol Chem1979，254，12： | 1 |
| 4 | A study on appraisal system of open space suitability of city square in Luoyang显示文摘 | Li D S YanY F Li L | 2011 | Applied Mechanics and Materials2011，，： | 1 |
| 5 | p53-Rb signaling pathway is involved in tubular cell senescence in renal ischemia/ reperfusion inj ury显示文摘 | Kailong L Du X Yani H | 2007 | Biocell2007，31，2： | 1 |
| 6 | Secretory vesicles are preferentially targeted to areas of low molecular SNARE density显示文摘 | Yany L Dun AR Martin K J | 2012 | PLOS One2012，7，49： | 1 |
| 7 | p53-Rb signaling pathway is involved in tubular cell senescence in renal ischemia/reperfusion injury 显示文摘 | Kailong L Du X Yani H | 2007 | Biocell2007，31，2： | 1 |
| 8 | Body size, recreational physical activity, and B-cell non-Hodgkin's lymphoma risk among women in the California teachers study显示文摘 | Yani L Prescott J Sullivan-Halley J | 2009 | Am J Epidemiol2009，170，10： | 1 |
| 9 | The efficacy of a voice training program:a case-control study in China显示文摘 | DUAN J M ZHU L YANY | 2010 | Eur Arch Otorhinolaryngol2010，267，： | 1 |
| 10 | p532Rb signaling pathwayis involved intubular cell senescence in renal ischemia/reperfusion injury显示文摘 | Kailong L Du X Yani H | 2007 | Bio-cell2007，31，2： | 1 |
| 11 | Knowledge map-based method for domain knowledge browsing 显示文摘 | HAOJ YANY GONG L WANG GX LINJJ | 2014 | Deci- sion Support Systems2014，61，： | 1 |
| 12 | Investigation of batchwise nitrite build-up by an enriched nitrification culture 显示文摘 | Yany L Alleman J E | 1992 | Wat Sci Tech1992，26，56： | 1 |
| 13 | Anattempt to reduce collapse through introducing cell wall deformations显示文摘 | Yany J L | 1998 | Wood and Fiber Science1998，30，1： | 1 |
| 14 | Circulating levels of adipo- kines and IGF-1 are associated with skeletal muscle strength of young and old healthy subjects显示文摘 | Bucci L Yani SL Fabbri C | 2013 | Biogerontology2013，14，3： | 1 |
| 15 | Friction pressure drop of R-12 in small hydraulic diameter extruded aluminum tubes with and without micro-fins 显示文摘 | YANY C Y WEBB R L | 1996 | International Journal of Heat and Mass Transfer1996，39，4： | 1 |
| 16 | P53-Rb signaling pathway is involved in tubular cell senescence in renal ischemia/reperfusion injury 显示文摘 | Kailong L Du X Yani H | 2007 | Biocell2007，31，2： | 1 |
| 17 | Investigation of batchwise nitrite build-up by an enriched nitrification culture显示文摘 | Yany L Alleman J E | 1992 | Water Science&Technology1992，26，56： | 1 |
| 18 | Variations of six transmembrane epithelial antigen of prostate 4 (STEAP4)gene are associated with metabolic syndrome in a female Uygur general population显示文摘 | Nanfang L Yanying G Hongmei W | | 0，，： | 1 |
| 19 | Economic Performance of In- dustrial Development on Collective Land in the Urbanization Process in Chi- na: Empirical Evidence From Shenzhen 显示文摘 | Lennon H Yani L Waiming L | 2013 | Habitat International2013，40，18： | 1 |
| 20 | A nanobody-based blocking enzyme-linked immunosorbent assay for detecting antibodies against pseudorabies virus glycoprotein E显示文摘Pseudorabies(PR)is an acute infectious disease of pigs caused by the PR virus(PRV)and results in great economic losses to the pig industry worldwide.PRV glycoprotein E(gE)-based enzyme-linked immunosorbent assay(ELISA)has been used to distinguish gE-deleted vaccine-immunized pigs from wild-type virus-infected pigs to eradicate PR in some countries.Nanobody has the advantages of small size and easy genetic engineering and has been a promising diagnostic reagent.However,there were few reports about developing nanobody-based ELISA for detecting anti-PRV-gE antibodies.In the present study,the recombinant PRV-gE was expressed with a bacterial system and used to immunize the Bactrian camel.Then,two nanobodies against PRV-gE were screened from the immunized camel by phage display technique.Subsequently,two nanobody-HRP fusion proteins were expressed with HEK293T cells.The PRV-gE-Nb36-HRP fusion protein was selected as the probe for developing the blocking ELISA(bELISA)to detect anti-PRV-gE antibodies.Through optimizing the conditions of bELISA,the amount of coated antigen was 200 ng per well,and dilutions of the fusion protein and tested pig sera were separately 1:320 and 1:5.The cut-off value of bELISA was 24.20%,and the sensitivity and specificity were 96.43 and 92.63%,respectively.By detecting 233 clinical pig sera with the developed bELISA and a commercial kit,the results showed that the coincidence rate of two assays was 93.99%.Additionallly,epitope mapping showed that PRV-gE-Nb36 recognized a conserved conformational epitope in different reference PRV strains.Simple,great stability and low-cost nanobody-based bELISA for detecting anti-PRV-gE antibodies were developed.The bELISA could be used for monitoring and eradicating PR. | Huanhuan Lü Pinpin Ji Siyu Liu Ziwei Zhang Lei Wang Yani Sun Baoyuan Liu Lizhen Wang Qin Zhao | 2024 | Journal of Integrative Agriculture2024，23，4： | 0 |
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