3篇
您的检索式：作者名="Yaping Cen"
|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Cryo-EM structures of PAC1 receptor reveal ligand binding mechanism显示文摘The pituitary adenylate cyclase-activating polypeptide type I receptor(PAC1R)belongs to the secretin receptor family and is widely distributed in the central neural system and peripheral organs.Abnormal activation of the receptor mediates trigeminovascular activation and sensitization,which is highly related to migraine,making PAC1R a potential therapeutic target.Elucidation of PAC1R activation mechanism would benefit discovery of therapeutic drugs for neuronal disorders.PAC1R activity is governed by pituitary adenylate cyclase-activating polypeptide(PACAP),known as a major vasodilator neuropeptide,and maxadilan,a native peptide from the sand fly,which is also capable of activating the receptor with similar potency.These peptide ligands have divergent sequences yet initiate convergent PAC1R activity.It is of interest to understand the mechanism of PAC1R ligand recognition and receptor activity regulation through structural biology.Here we report two near-atomic resolution cryo-EM structures of PAC1R activated by PACAP38 or maxadilan,providing structural insights into two distinct ligand binding modes.The structures illustrate flexibility of the extracellular domain(ECD)for ligands with distinct conformations,where ECD accommodates ligands in different orientations while extracellular loop 1(ECU)protrudes to further anchor the ligand bound in the orthosteric site.By structureguided molecular modeling and mutagenesis,we tested residues in the ligand-binding pockets and identified clusters of residues that are critical for receptor activity.The structures reported here for the first time elucidate the mechanism of specificity and flexibility of ligand recognition and binding for PACT R,and provide insights toward the design of therapeutic molecules targeting PAC1R. | Jia Wang Xianqiang Song Dandan Zhang Xiaoqing Chen Xun Li Yaping Sun Cui Li Yunpeng Song Yao Ding Ruobing Ren Essa Hu Harrington Liaoyuan AHu Wenge Zhong Cen Xu Xin Huang Hong-Wei Wang Yingli Ma | 2020 | Cell Research2020，30，5： | 1 |
| 2 | Author Correction: Cryo-EM structures of PAC1 receptor reveal ligand binding mechanism显示文摘We noticed the missing structure deposition information in our manuscript published online on February 11,2020,which are the PDB codes and EMDB map entries of the structure PACAP38-PAC1R-GS(PDB code:6M1I and EMDB entry:EMD-30048)and maxadilan-PAC1R-Gs(PDB code:6M1H and EMDB entry:EMD-30047).We apologize for any inconvenience this missing information has caused.This correction does not affect the description of the results or the conclusion of this work. | Jia Wang Xianqiang Song Dandan Zhang Xiaoqing Chen Xun Li Yaping Sun Cui Li Yunpeng Song Yao Ding Ruobing Ren Essa Hu Harrington Liaoyuan AHu Wenge Zhong Cen Xu Xin Huang Hong-Wei Wang Yingli Ma | 2020 | Cell Research2020，30，5： | 0 |
| 3 | Micropore-confined Ru nanoclusters catalyst for efficient pHuniversal hydrogen evolution reaction显示文摘Pt-based catalysts are used commercially for the hydrogen evolution reaction(HER),even though the low earth abundance and high cost of platinum hinder scale-up applications.Ru metal is a promising alternative catalyst for HER owing to its lower cost but similar metal-hydrogen bond strength to Pt.However,designing an efficient and robust Ru-based electrocatalyst for pHuniversal HER is challenging.Herein,we successfully synthesized N-doped carbon(NC)supported ruthenium catalysts with different Ru sizes(single-atoms,nanoclusters and nanoparticles),and then systematically evaluated their performance for HER.Among these catalysts,the Ru nanocluster catalyst(Ru NCs/NC)displayed optimal catalytic performance with overpotentials of only 14,30,and 32 mV(at 10 mA·cm^(-2))in 1 M KOH,1 M phosphate buffer saline(PBS),and 0.5 M H_(2)SO_(4),respectively.The corresponding mass activities were 32.2,12.1 and 8.1 times higher than those of 20 wt.%Pt/C,and also much better than those of the Ru single-atoms(Ru SAs/NC)and Ru nanoparticle(Ru NPs/NC)catalysts,at an overpotential of 100 mV under alkaline,neutral and acidic conditions,respectively.Density functional theory(DFT)calculations revealed that the outstanding HER performance of the Ru NCs/NC catalyst resulted from a strong interaction between the Ru nanoclusters and the N-doped carbon support,which downshifted the d-band center and thus weakened the*H adsorption ability of Ru sites. | Xiaoxiao Huang Ruihu Lu Yaping Cen Dunchao Wang Shao Jin Wenxing Chen Geoffrey I NWaterhouse Ziyun Wang Shubo Tian Xiaoming Sun | 2023 | Nano Research2023，16，7： | 0 |
您所在的机构暂未开通此部分的功能，请联系管理员