|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Approaching Malaria Elimination in China显示文摘Malaria has been one of the most important public health problems over China and world.When dated back to the mid-20th century,malaria seriously affected the health of Chinese people with widespread epidemics.About 30 million malaria cases with an estimated 1%mortality rate were recorded in China each year in the 1940s,which caused an extremely high disease burden and a toll on the labor force(1–2).Even as recently as the 1970s,a peak in the incidence of malaria was recorded with 24.1 million cases(1–2). | Ning Xiao Qiuli Xu Jun Feng Zhigui Xia Lei Duan Duoquan Wang Yayi Guan Xiao-nong Zhou | 2020 | China CDC weekly2020，2，17： | 2 |
| 2 | Formalin-induced astrocyte glutamate-glutamine cycle involved in rat spinal cord central sensitization显示文摘BACKGROUND:Central sensitization,a state of increased excitability of nociceptive neurons in the spinal dorsal horn following peripheral tissue injury and/or inflammation,is an important mechanism underlying hyperalgesia and neuropathic pain.Participation of the glutamate-glutamine cycle in central sensitization of the spinal cord remains poorly understood. OBJECTIVE:To determine whether the astrocyte-neuronal glutamate-glutamine cycle is involved in formalin-induced central sensitization in the spinal cord. DESIGN,TIME AND SETTING:A randomized,controlled,animal experiment was performed at the Institute of Orthopedics,Second Hospital,Lanzhou University,China from September 2007 to August 2008. MATERIALS:Methionine sulfoximine(MSO,0.1 mmol/L),glutamine(0.25 mmol/L),and formalin were used for this study. METHODS:A total of 43 male,Sprague Dawley rats,aged 4 months,were randomly assigned to a sham operation group(n = 6) and a model group(n = 37).Rats in the model group received intrathecal infusion in the spinal cord.7 days later,37 model rats were randomly divided into PBS, MSO,glutamine,MSO + glutamine and formalin subcutaneous injection alone groups.The PBS, MSO,glutamine,MSO + glutamine groups were respectively intrathecally injected with PBS,MSO, glutamine,MSO + glutamine(50μL each),and then infused with 10μL of saline.Rats from the sham operation group were not subjected to intrathecal infusion in the spinal cord.At 15 minutes after intrathecal injection,a rat model of formalin-induced inflammatory pain was established by subcutaneous injection of 5%formalin(50μL) in the left hindpaw. MAIN OUTCOME MEASURES:Changes in spontaneous nociceptive behavior(licking/biting or flinching) were observed following formalin injection into the rat hindpaw. RESULTS:Compared with the PBS group,duration of licking/biting was significantly shortened,and flinching frequency was significantly diminished in the MSO group(P<0.05).Compared with the MSO group,duration of licking/biting was significantly prolonged,and flinching frequency was significantly increased in the MSO + glutamine group(P<0.05).There was no significant difference in inflammatory pain behaviors among the sham operation,PBS,glutamine,MSO + glutamine,and formalin subcutaneous injection alone groups(P>0.05). CONCLUSION:The astrocyte-neuronal glutamate-glutamine cycle in the spinal cord was shown to be involved in central sensitization induced by formalin subcutaneous injection into the hindpaw. | Haili Shen Yufeng XieO Hua Han Yayi Xia Youcheng Zhang Cuifang Wang Jing Wang | 2009 | Neural Regeneration Research2009，4，11： | 2 |
| 3 | Comparison Study of Corrosion Behavior and Biocompatibility of Polyethyleneimine (PEI)/Heparin and Chitosan/Heparin Coatings on NiTi alloy显示文摘Biomedical molecule has received extensive application in surface modification coating.In this paper,polyethyleneimine (PEI)/heparin coating and chitosan/heparin coating on NiTi alloy substrates were prepared by layer-by-layer method,and differences of corrosion behavior and biocompatibility of the coatings were studied.Static contact angles and Fourier transform infrared (FT-IR) test were used to analysis the surface morphology and structure character of the materials,and atomic absorption spectrum was used to verify the corrosion behavior of the materials.Furthermore,hemolysis,dynamic clotting time and platelet binding test were utilized to investigate the biocompatibility of the materials.The results revealed that the blood compatibility of PEI/heparin coating is better than that of the chitosan/heparin coating as well as the substrate. | Ping Dong Weichang Hao Yayi Xia Guozu Da Tianmin Wang | 2010 | Journal of Materials Science & Technology2010，26，11： | 1 |
| 4 | Possible effect of fluid shear stress on osteoclastogenesis显示文摘Bone remodeling is performed under the joint action of osteoblasts and osteoclasts. Since the effect of osteoclasts has been gradually recognized on bone and joint diseases, targeted researches toward osteoclasts have become a hot research field. This article reviews the relevant medical literature concerning the possible effects of the fluid shear stress (FSS) on the osteoclastogenesis chiefly from the aspects of RANKL-RANK-OPG system, the macrophage colony-stimulating factor (M-CSF), and calcitonin receptor (CTR). On the basis of the changes of the expression of osteoclastic activities, it is suggested that FSS is a potent, important regulator of bone metabolism. | Tong Xiaoyu Xia Yayi | 2010 | Journal of Medical Colleges of PLA(China)2010，25，2： | 1 |
| 5 | Ependymal cell proliferation and apoptosis following acute spinal cord injury in the adult rat显示文摘BACKGROUND: Studies have reported that spinal cord injury can induce the reactive proliferation of ependymal cells and secondarily cause the apoptosis of nerve cells. However, there is no generally accepted theory on the apoptotic characteristics of ependymal cells in the injured spinal cord. OBJECTIVE: To observe the reactive proliferation and apoptosis of ependymal cells in adult rats following acute spinal cord injury. DESIGN, TIME AND SETTING: A randomized control study based on neuropathology was performed in the Third Military Medical University of Chinese PLA between 2005 and 2007. MATERIALS: Forty healthy, adult, Wistar rats were included in the present study. METHODS: Moderate spinal cord injury was established in twenty rats using Feeney's method, while the remaining 20 rats served as controls and were only treated with laminectomy. All rats were injected intraperitoneally with 1.25 mL of BrdU solution (10 mg BrdU/mL saline) 3 times at 4 hours intervals during the 12 hours prior to sacrifice. MAIN OUTCOME MEASURES: Ependymal cell proliferation and apoptosis in the rat spinal cord were determined by BrdU and nestin immunofluorescence double-labeling, as well as the TUNEL method, at 1, 3, 7, and 14 days after operation. RESULTS: In the moderate spinal cord injury rats, nestin expression was observed in the cytoplasm of ependymal cells. One day immediately following surgery, ependymal cells were BrdU-labeled. The number of BrdU-positive cells increased at 3 days, reached a peak at 7 days, and gradually reduced thereafter. The ependyma developed from a constitutive monolayer cells to a multi-layer cell complex. Some BrdU/Nestin double-positive ependymal cells migrated out from the ependyma. TUNEL-positive cells were also detected in the ependyma in the central region, as well as ischemic regions of the injured spinal cord. In addition, TUNEL-positive cells were visible in the ependyma. No TUNEL-positive ependymal cells were observed in the normal spinal cord. CONCLUSION: Proliferating ependymal cells induced apoptosis in the central and surrounding region following spinal cord injury. | Xu Wang Jun Qian Yanchao Ma Guoxin Nan Shuanke Wang Yayi Xia Youcheng Zhang | 2008 | Neural Regeneration Research2008，3，10： | 1 |
| 6 | Astroglial glutamate-glutamine cycle is involved in the modulation of inflammatory nociception in rats显示文摘Our previous behavioral studies have indicated that the astroglial glutamate-glutamine cycle is involved in the process of formalin-induced spinal cord central sensitization,but there was little morphological evidence.In this study,double-labeling immunofluorescence techniques showed that after rats were intrathecally injected with PBS and plantarly injected with formalin,glial fibrillary acidic protein(GFAP) and glutamine synthesase(GS) expression were increased and GFAP/GS coexpression was changed to include layers III and IV.After intrathecal injection of methionine sulfoximine,a GS specific inhibitor,the formalin-induced change in expression and coexpression of GFAP and GS in spinal cord dorsal horns was inhibited.The morphology,distribution and quantity of astrocytes recovered to normal levels.An intrathecal glutamine injection reversed the inhibitory effect of methionine sulfoximine.Astrocytes showed significant activation and distribution extended to layers V and VI.The present study provides morphological evidence that the astroglial glutamateglutamine cycle is involved in the process of formalin-induced spinal cord central sensitization. | Tiancheng Wang Jing Wang Bin Geng Hongyu Guo Haili Shen Yayi Xia | 2011 | Neural Regeneration Research2011，6，35： | 0 |
| 7 | Neuroprotective effect of estrogen after chronic spinal cord injury in ovariectomized rats显示文摘BACKGROUND: At present, there is still lack of effective drugs for chronic spinal cord injury, whereas it is found recently that estrogen has a neuroprotective effect on brain and spinal cord injuries. OBJECTIVE: To observe the effect of estrogen on the apoptosis of nerve cells after gradual chronic spinal cord injury in ovariectomized rats. DESIGN: A randomized controlled animal trial. SETTING: Institute of Orthopaedics, the Second Hospital of Lanzhou University. MATERIALS: Sixty-five female Wistar rats of common degree, weighing 220-250 g, were provided by the experimental animal center of Lanzhou University. The rats were randomly divided into sham-operated group (n =5), estrogen-treated group (n =30) and saline control group (n =30), and the latter two groups were observed at 1, 3, 7, 14, 28 and 60 days respectively, and 5 rats for each time point. METHODS: All the rats were treated with bilateral oophorectomy 2 weeks before the experiment. T10 vertebral lamina was revolved into using plastic screw. The spinal canal impingement was not induced initially. After that, the original incision was opened to expose the screw every 7-10 days. MAIN OUTCOME MEASURES: The apoptosis and Caspase-3 positive cells in the damaged spinal cord were detected using terminal deoxynucleotidal transferase-mediated dUTP-biotin nick end labeling (TUNEL) method and Caspase-3 immunohistochemical staining at 1, 3, 7, 14, 28 and 60 days after chronic spinal cord injury respectively. RESULTS: Totally 65 rats were used, and the deleted ones during the experiment were supplemented by others. Changes of Caspase-3 expression after spinal cord injury: In the sham-operated group, only a small amount of Caspase-3 proteins were observed in the rat spinal cord, mainly located in motor neurons of spinal cord anterior horn. In the estrogen-treated group and saline control group, positive cells expressed occasionally at 1 day postoperatively, began to increase obviously at 7 days after injury, strongly expressed at 14 and 28 days, but decreased at 60 days, mainly located in the neurons of spinal cord gray matter anterior horn, and they expressed fewer in the motor neurons and white matter of ventral horn, and there were obvious differences between the estrogen-treated group and saline control group at 7, 14, 28 and 60 days (P < 0.05). CONCLUSION: Estrogen can reduce the apoptosis of nerve cells and promote the recovery of neurological function following gradual chronic spinal cord injury. | Yanhong Li Shuanke Wang Yayi Xia Jing Wang Wenjie Pan Yingbin Shi Mige Wang | 2007 | Neural Regeneration Research2007，2，8： | 0 |
您所在的机构暂未开通此部分的功能，请联系管理员