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| 1 | Celastrus orbiculatus extract induces mitochondrial-mediated apoptosis in human hepatocellular carcinoma cells显示文摘OBJECTIVE:To investigate the apoptotic effects and underlying molecular mechanisms of Celastrus orbiculatus(C.orbiculatus) extract in human hepatocellular carcinoma cells.METHODS:Human hepatocellular carcinoma cells(HCCLM6) were treated with C.orbiculatus extract(COE) at different nontoxic concentrations(10,20,40,80,and 160 μg/mL).The effect of COE on HC-CLM6 viability was examined using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide(MTT) assays.Cellular apoptosis following COE treatment was assessed by flow cytometry and western blot analysis.RESULTS:COE significantly inhibited cell viability and induced apoptosis of HCCLM6 cells in a dose-dependent manner.Apoptosis was accompanied by increased Bax expression and decreased Bcl-2 expression.In addition,COE treatment led to the release of cytochrome c,activation of caspase-3,and cleavage of poly(ADP-ribose) polymerase(PARP).Furthermore,activation of extracellular signal-regulated kinase(ERK),p38 kinase,and c-Jun N-terminal kinase(JNK) phosphorylation,and down-regulation of Akt phosphorylation was observed.CONCLUSION:COE induces mitochondrial-mediated,caspase-dependent apoptosis in HCCLM6 cells,which might be attributed to the activation of mitogen-activated protein kinase(MAPK) and inhibition of Akt signaling pathways.These data suggest that COE may be a potential treatment for human hepatocellular carcinoma. | Hua Zhang Yayun Qian Yanqing Liu Guoqing Li Pingfang Cui Yaodong Zhu Hui Ma Xue Ji Shiyu Guo Hisamits Tadashi | 2012 | Journal of Traditional Chinese Medicine2012，32，4： | 18 |
| 2 | A LysM Receptor Heteromer Mediates Perception of Arbuscular Mycorrhizal Symbiotic Signal in Rice显示文摘Symbiotic microorganisms improve nutrient uptake by plants.To initiate mutualistic symbiosis with arbus-cular mycorrhizal(AM)fungi,plants perceive Myc factors,including lipochitooligosaccharides(LCOs)and short-chain chitooligosaccharides(CO4/CO5),secreted by AM fungi.However,the molecular mechanism of Myc factor perception remains elusive.In this study,we identified a heteromer of LysM receptor-like kinases consisting of OsMYR1/OsLYK2 and OsCERK1 that mediates the perception of AM fungi in rice.CO4 directly binds to OsMYR1,promoting the dimerization and phosphorylation of this receptor complex.Compared with control plants,Osmyr1 and Oscerk1 mutant rice plants are less sensitive to Myc factors and show decreased AM colonization.We engineered transgenic rice by expressing chimeric receptors that respectively replaced the ectodomains of OsMYR1 and OsCERK1 with those from the homologous Nod factor receptors MtNFP and MtL YK3 of Medicago truncatula.Transgenic plants displayed increased cal-cium oscillations in response to Nod factors compared with control rice.Our study provides significant mechanistic insights into AM symbiotic signal perception in rice.Expression of chimeric Nod/Myc recep-tors achieves a potentially important step toward generating cereals that host nitrogen-fixing bacteria. | Jiangman He Chi Zhang Huiling Dai Huan Liu Xiaowei Zhang Jun Yang Xi Chen Yayun Zhu Dapeng Wang Xiaofeng Qi Weichao Li Zhihui Wang Guoyong An Nan YU Zuhua He Yong-Fei Wang Youli Xiao Peng Zhang Ertao Wang | 2019 | Molecular Plant2019，12，12： | 9 |
| 3 | U-shaped association between telomere length and esophageal squamous cell carcinoma risk: a case-control study in Chinese population显示文摘在由维持 chromosomal 正直并且阻止染色体的生物变老的一个关键角色结束的 Telomeres 玩熔化。流行病学的研究建议了 telomere 长度的内部个人的差别能影响倾向到多重癌症,但是关于食道的有鳞的房间癌(ESCC ) 的证据仍然是不明确的。几 telomere 在白种人的长度相关的单个核苷酸多型性(TLSNP ) 在染色体宽的协会研究被报导了。然而,在 ESCC 开发的 telomere 长度和 TL-SNPs 的效果是不清楚的。因此,我们进行了盒子控制研究(1045 个 ESCC 案例和 1433 控制) 在中国人口评估在 telomere 长度, TL-SNPs,和 ESCC 风险之间的协会。作为结果, ESCC 案例显示出全面更短的相对 telomere 长度(RTL )( 中部:1.34 ) 比控制(中部:1.50, P < 0.001 ) 。更有趣地,一个明显的非线性的U字形的协会在 RTL 和 ESCC 风险之间被观察( P < 0.001 )与比率(95%信心间隔)等于到 2.40 的机会( 1.843.14 ), 1.36 ( 1.031.79 ), 1.01 ( 0.761.35 ),并且 1.37 ( 1.031.82 )为个人在第一(最短),第二,第三,并且 第5 (最长) quintile 分别地,在是的 第4 quintile 与那些相比引用组。没有重要协会在八报导 TL-SNPs 和 ESCC 危险性之间被观察。这些调查结果建议短或极其长的 telomeres 可以是为在中国人口的 ESCC 的风险因素。 | Jiangbo Du Wenjie Xue Yong Ji Xun Zhu Yayun Gu Meng Zhu Cheng Wang Yong Gao Juncheng Dai Hongxia Ma Yue Jiang Jiaping Chen Zhibin Hu Guangfu Jin Hongbing Shen | 2015 | Frontiers of Medicine2015，9，4： | 4 |
| 4 | The cancer-testis gene,MEIOB,sensitizes triple-negative breast cancer to PARP1 inhibitors by inducing homologous recombination deficiency显示文摘Objective:The newly defined cancer-testis(CT)gene,MEIOB,was previously found to play key roles in DNA double-strand break(DSB)repair.In this study,we aimed to investigate the effects and mechanisms of MEIOB in the carcinogenesis of triple-negative breast cancers(TNBCs).Methods:The Cancer Genome Atlas database was used to quantify the expression of MEIOB.Cox regression analysis was used to evaluate the association between MEIOB expression and the prognosis of human TNBC.The effects of MEIOB on cell proliferation and migration in TNBCs were also assessed in vitro.Patient-derived xenograft(PDX)models were used to assess the sensitivity of breast cancers with active MEIOB to PARP1 inhibitors.Results:We confirmed MEIOB as a CT gene whose expression was restricted to the testes and breast tumors,especially TNBCs.Its activation was significantly associated with poor survival in breast cancer patients[overall,hazard ratio(HR)=1.90(1.16–2.06);TNBCs:HR=7.05(1.16–41.80)].In addition,we found that MEIOB was oncogenic and significantly promoted the proliferation of TNBC cells.Further analysis showed that MEIOB participated in DSB repair in TNBCs.However,in contrast to its function in meiosis,it mediated homologous recombination deficiency(HRD)through the activation of poly ADP-ribose polymerase(PARP)1 by interacting with YBX1.Furthermore,activated MEIOB was shown to confer sensitivity to PARP inhibitors,which was confirmed in PDX models.Conclusions:MEIOB played an oncogenic role in TNBC through its involvement in HRD.In addition,dysregulation of MEIOB sensitized TNBC cells to PARP inhibitors,so MEIOB may be a therapeutic target of PARP1 inhibitors in TNBC. | Yayun Gu Cheng Wang Rongxuan Zhu Jianshui Yang Wenwen Yuan Yanhui Zhu Yan Zhou Na Qin Hongbing Shen Hongxia Ma Hongxia Wang Xiaoan Liu Zhibin Hu | 2021 | Cancer Biology & Medicine2021，18，1： | 2 |
| 5 | 汨罗屈原博物馆二期——楚辞文化交流中心设计,岳阳,湖南显示文摘“楚辞文化交流中心”为汨罗屈原博物馆二期工程,位于一期的屈子书院东北200m处的一座山丘上。设计顺应丘陵地形灵活多变地形成空间布局,主要包括:1)楚辞堂:由中央多功能厅、环廊、二层展廊等构成;2)展馆东区:由东展厅、东庭、报告厅、研习室、馆藏室等构成;3)展馆西区:由接待厅、餐厅、西庭、办公空间等构成。 | 汤朔宁 ZHU Yayun(译) | 2022 | 世界建筑2022，，10： | 1 |
| 6 | 关麓书院实施方案设计,黄山,安徽显示文摘(1)村落时空脉络黄山黟县碧阳镇西武乡的关麓村,是古徽州一个亦儒亦商的风土聚落,因村西南西屏山和武亭山相交处的西武关而得名。一条南连祁门北接池州,由西武关向东北延伸的官路(驿道),穿村而过。源自武亭山的“下溪”(武溪)紧贴村东边缘,并沿“月塘”北行。后唐起汪姓在黟繁衍,明成化年间汪姓在关麓村兴起,至清乾隆时乡绅汪昭敩房支兴旺,称“关麓八大家”,其宗祠——承德堂在官路南侧,抵近月塘。1994年清华大学陈志华教授率队首次考察关麓村,后出版《关麓村》一书。 | 周榕 ZHU Yayun(译) | 2022 | 世界建筑2022，，10： | 1 |
| 7 | Interactive Visual Analysis on the Attack and Defense Drill of Grid Cyber-physical Systems显示文摘The open and distributed connection of the powersystem makes it vulnerable to various potential cyber-attacks,which may lead to power outages and even casualties. Therefore,the construction of attack and defense drill (ADD) platforms forattack mechanism investigation and protection strategy evaluationhas become a research hotspot. However, for the massiveand heterogeneous security analysis data generated during thedrill, it is rare to have a comprehensive and intuitive methodto visually and efficiently display the perspective of the attackerand defender. In order to solve this problem, this paper proposesa visual analysis scheme of an ADD framework for a grid cyberphysicalsystem (GCPS) based on the interactive visual analysismethod. Specifically, it realizes system weakness discovery basedon knowledge visualization, optimization of the detection modeland visualization interaction. Finally, the case study on thesimulation platform of ADD proves the effectiveness of theproposed method. | Kehe Wu Jiawei Li Yayun Zhu Siwei Miao Sixun Zhu Chunjie Zhou | 2021 | CSEE Journal of Power and Energy Systems2021，7，1： | 1 |
| 8 | Gene amplification-driven RNA methyltransferase KIAA1429 promotes tumorigenesis by regulating BTG2 via m6A-YTHDF2-dependent in lung adenocarcinoma显示文摘Background:Epigenetic alterations have been shown to contribute immensely to human carcinogenesis.Dynamic and reversible N6-methyladenosine(m6A)RNA modification regulates gene expression and cell fate.However,the reasons for activation of KIAA1429(also known as VIRMA,an RNA methyltransferase)and its underlying mechanism in lung adenocarcinoma(LUAD)remain largely unexplored.In this study,we aimed to clarify the oncogenic role of KIAA1429 in the tumorigenesis of LUAD.Methods:Whole-genome sequencing and transcriptome sequencing of LUAD data were used to analyze the gene amplification of RNA methyltransferase.The in vitro and in vivo functions of KIAA1429 were investigated.Transcriptome sequencing,methylated RNA immunoprecipitation sequencing(MeRIP-seq),m6A dot blot assays and RNA immunoprecipitation(RIP)were performed to confirm the modified gene mediated by KIAA1429.RNA stability assays were used to detect the half-life of the target gene.Results:Copy number amplification drove higher expression of KIAA1429 in LUAD,whichwas correlatedwith poor overall survival.Manipulating the expression of KIAA1429 could regulate the proliferation and metastasis of LUAD.Mechanistically,the target genes of KIAA1429-mediated m6A modification were confirmed by transcriptome sequencing and MeRIP-seq assays.We also revealed that KIAA1429 could regulate BTG2 expression in an m6A-dependent manner.Knockdown of KIAA1429 significantly decreased the m6A levels of BTG2 mRNA,leading to enhanced YTH m6A RNA binding protein 2(YTHDF2,the m6A“reader”)-dependent BTG2 mRNA stability and promoted the expression of BTG2;thus,participating in the tumorigenesis of LUAD.Conclusions:Our data revealed the activation mechanism and important role of KIAA1429 in LUAD tumorigenesis,which may provide a novel view on the targeted molecular therapy of LUAD. | Chang Zhang Qi Sun Xu Zhang Na Qin Zhening Pu Yayun Gu Caiwang Yan Meng Zhu Juncheng Dai ChengWang Ni Li Guangfu Jin Hongxia Ma Zhibin Hu Erbao Zhang Fengwei Tan Hongbing Shen | 2022 | Cancer Communications2022，42，7： | 1 |
| 9 | 骑楼街区再生工程设计,海口,海南显示文摘海口骑楼街区建于1920-1930年代,源于19世纪末产生于南洋地区有着“五尺街”骑廊和地方化“巴洛克”饰面的商住街区,面积68hm^(2),是中国现存面积最大、保存最完整的骑楼街区。经过90余载的沧桑变迁,海口骑楼老街奇丽外表的内里已衰朽不堪,街廓风貌破败严重。1992年夏,常青率同济建筑系实习学生赴海口测绘骑楼老街,做了风貌保护专题研究。 | 张杰 ZHU Yayun | 2022 | 世界建筑2022，，10： | 0 |
| 10 | 月湖西区(一期)再生工程设计,宁波,浙江显示文摘宁波老城西南的月湖地段,是南宋浙东“四明学派”的交集处。月湖西区北片东临偃月街,北至中山西路,西和南靠三板桥街及青石街,是不可多得的重要历史文化街区。在2010年前后的旧城改造中,当地对这片占地约1.9hm~2的历史文化街区进行了拆改重建,使其历史空间肌理几近解体。2012年,同济大学常青团队接受了恢复月湖西区北片的再生设计任务。 | 董丹申 ZHU Yayun(译) | 2022 | 世界建筑2022，，10： | 0 |
| 11 | 来氏聚落再生规划设计,杭州,浙江显示文摘杭州来氏聚落位于钱塘江南岸的长河古镇中心地段,自宋明以来产生过许多名仕鸿儒,和以“九厅十三堂”为代表的宗族人文景观。经过20世纪后半叶以来的时代大潮涤荡,这个望族聚落今已衰朽不堪,面临着艰难迈向城镇化和快速丧失风土资源的双重现实。1996年长河镇从萧山县划入杭州市滨江区,2002年常青率团队接受了长河来氏聚落保护与再生规划设计委托。设计策略的核心是结构性保护和适应性再生,可概括为:延续地志——基于史地维度的聚落所在地貌和地景重塑;保持地脉——基于环境结构的聚落肌理和空间关系梳理;保留地标——基于文化记忆的聚落建筑保存修复和活化再生。 | 常青 ZHU Yayun | 2022 | 世界建筑2022，，10： | 0 |
| 12 | 黄岩委羽公园工程设计,台州,浙江显示文摘道教“第二洞天”所在的台州黄岩老城南郊委羽山公园设计项目,建筑占地仅略大于10,000m^(2),其余为山水和硬地。2004年常青主持完成位于黄岩老城中心的孔庙及周边地段保护规划设计,2016年重返黄岩主持孔庙地段工程设计并配合实施,同时率团队接受了委羽山公园的设计任务。 | 李兴钢 ZHU Yayun(译) | 2022 | 世界建筑2022，，10： | 0 |
| 13 | 郑州商城保护地段城市设计,郑州,河南显示文摘郑州商代王城是代表中国国家历史身份的八大古都之一,也是迄今我国已发现规模最大、保存最完好的商代都城城垣遗址,是华夏3600年文明史和都城筑城史的见证,其遗产本体需要高水准保护和展示,其比邻街区需要适应性活化和再生。因此本设计的总体定位为“中华古都的现代复兴”,包括古韵新风的风格定位和商都特色的功能定位,以突显郑州历史身份载体的商遗址价值,建构街区与宗教文物相连的名胜地段,创造街区观游购三位一体的活力场所,促进中心城区的空间再生与文化复兴。 | 周俭 ZHU Yayun(译) | 2022 | 世界建筑2022，，10： | 0 |
| 14 | 外滩轮船招商总局大楼修复工程设计,上海显示文摘外滩九号“轮船招商总局大楼”建于1901年,是在原旗昌洋行临江花园的基址上建造的,是外滩建筑群中唯一一座新古典风格的外廊式建筑。原建筑由英国玛礼逊洋行设计。建筑主体为3层砖、石、木、钢4种材料混用的结构。百年沧桑,物是形非,包括南北两翼山花的整个坡顶屋面被拆除,新古典柱式构成的敞廊被简陋厂房般的玻璃面钢窗封死,红砖墙被水泥砂浆抹平。在建成100年后,2001年常青教授在主持“外滩源”立项规划和概念设计的同时,领衔对这一外滩建筑做了保护再生设计。 | 沈迪 ZHU Yayun(译) | 2022 | 世界建筑2022，，10： | 0 |
| 15 | 桑珠孜宗堡复原工程设计,日喀则,西藏显示文摘西藏日喀则的桑珠孜宗堡(当地亦称宗宫)曾是老城天际线的制高点,初建于元末,是全藏宗山建筑的典范,可能也是晚于其330年的拉萨布达拉宫祖型。“文革”初期,宗堡作为农奴制的象征被捣毁,木结构主体已无存,仅剩石墙残墟。近40年后,在日喀则各界呼吁下,宗堡复原工程成为上海市最大的单项援藏项目。 | 陈同滨 ZHU Yayun(译) | 2022 | 世界建筑2022，，10： | 0 |
| 16 | 颐和路历史文化街区文物建筑修缮工程设计,南京,江苏显示文摘颐和路历史文化街区位于江苏省南京市鼓楼区,是中国首批公布的30个历史文化街区之一,其路网格局、街道和建筑风貌特色集中体现了民国时期南京《首都计划》的理念。11片区位于颐和路历史文化街区的南端,是街区复兴的首期实验区。片区包括由院墙分隔的10个独立院落和9栋鼓楼区不可移动文物建筑。院落内改加建普遍,历史风貌被破坏,开放空间被侵占,街区整体封闭性强、公共性低。 | 鲁安东 ZHU Yayun(译) | 2022 | 世界建筑2022，，10： | 0 |
| 17 | 汨罗屈原博物馆一期——屈子书院设计,岳阳,湖南显示文摘湖南汨罗的屈子书院历史上曾是国家重点文物保护单位屈子祠的组成部分,初建于宋大中祥符年间(1008-1017),故址在今汨罗城北。清乾隆十八年(1753),书院随屈子祠迁往汨罗江畔玉笥山现址,1960年代书院部分被拆除。屈子书院重建工程为国家重点文化工程,2010年,常青率团队承担了该工程的设计任务,并配合了全过程施工,2018年该项目竣工使用。 | 柳肃 ZHU Yayun(译) | 2022 | 世界建筑2022，，10： | 0 |
| 18 | 南海佛学院方案设计,三亚,海南显示文摘南海佛学院位于海南省三亚市南山文化旅游区内,东邻南山寺。基址傍山面海,形势左辅右弼,居中的北峰制高点为120m。2015年常青应邀率团队参加了设计方案征集。学院总体布局“一干三支”,呈狭长形展开。院总部位于北峰南坡,汉传、南传和藏传三传佛教的3个分院沿基地东西向一字排开,均有独立的禅堂、佛殿和教学楼,学员宿舍、食堂等附属建筑。另在院总部西侧山头上建可对外服务的佛教文化体验区和驿馆,并预留了后续发展空间。设计依山就势设置环形机动车道和路边停车场,以步行动线——“菩提林荫道”东西贯穿校园各分部,循等高线和山体坡度选择适宜的蹬道走向。 | 汪孝安 ZHU Yayun(译) | 2022 | 世界建筑2022，，10： | 0 |
| 19 | 龙门阁及周边景观实施方案设计,上海显示文摘“龙门阁”基地位于奉贤新城“上海之鱼”景观公园内,东依文耀路,南临浦南运河,西靠支流水道,北接秀竹路。2021年,常青教授领衔设计了藏书阅览属性的“龙门阁”实施方案,将其作为公园地标性建筑,辅以周边配套功能与相关业态建筑群,为奉贤增添一处具有历史人文意涵和现代城市功能的公益休闲场所。登阁人流从秀竹路和文耀路广场引入,汇聚于中央书吧厅和阁前广场;车辆由地块西北侧机动车坡道(原有机动车入口)至龙门阁西侧地面停车场,沿浦南运河北岸设置景观廊及滨水步道,与其他地块绿地相连。 | 章明 ZHU Yayun(译) | 2022 | 世界建筑2022，，10： | 0 |
| 20 | 大观楼方案设计,常州,江苏显示文摘历史上的大观楼是常州府衙前的门楼,故址在北大街西侧健身路与大观路相交处,始建于唐末,后毁,明正统重建。史载高逾百尺,登上可尽览周边常州风光,故号“三吴第一楼”,惜毁于民国初战火。尽管常州社会各界对重建大观楼的呼声不断,但由于历史图像资料的缺失和建成环境现状,已不可能在大观楼原址实现这一愿景了。但重建方案选址仍在老城厢西侧,较好的选择是在古运河与关河交汇的三角状城市公共绿地内,重建后的大观楼将集城市景观、竖向会展、文化消费于一体。 | 李振宇 ZHU Yayun(译) | 2022 | 世界建筑2022，，10： | 0 |
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