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| 1 | CDP1, a novel component of chloroplast division site positioning system in Arabidopsis显示文摘叶绿体是从 endosymbiotic cyanobacteria 演变的植物特定的细胞器。他们通过二进制分裂划分。叶绿体部门地点的选择为对称的叶绿体部门是枢轴的。在 E。coli,在房间的中点部门地点放被 Min 系统的动态摆动调整,它包括 MinC,头脑和矿。在植物的头脑和矿的相当或相同的事物涉及叶绿体分割。MinC 的相当或相同的事物仍然没在更高的植物被识别。然而,象 FtsZ 一样蛋白质, ARC3,被发现地点放涉及叶绿体分割。这里,我们报导放 1 的那个叶绿体部门地点(AtCDP1 ) 是在 Arabidopsis 涉及叶绿体部门地点放置的新奇叶绿体部门蛋白质。AtCDP1 被与房间部门显型为殖民地在细菌屏蔽一个 Arabidopsis cDNA 表示图书馆发现。AtCDP1 只在 Arabidopsis 在年轻绿纸巾被表示。有多重部门地点的伸长的叶绿体在 loss-of-function cdp1 异种被观察。AtCDP1 的 Overexpression 也引起了一个叶绿体部门显型。蛋白质相互作用试金建议 AtCDP1 可以调停通过和 ARC3 的相互作用放的叶绿体部门地点。总的来说,我们的结果显示 AtCDP1 是放系统,和这个系统的工作机制的叶绿体部门地点的一个新奇部件与在原核生物的房间的传统的 MinCDE 系统的不同。 | Min Zhang Yong Hu Jingjing Jia Dapeng Li Runjie Zhang Hongbo Gao Yikun He | 2009 | Cell Research2009，19，7： | 5 |
| 2 | A transceptor-channel complex couples nitrate sensing to calcium signaling in Ambidopsis显示文摘Nitrate-induced Ca^(2+) signaling is crucial for the primary nitrate response in plants.However,the molecular mechanism underlying the generation of the nitrate-specific calcium signature remains unknown.We report here that a cyclic nucleotide-gated channel(CNGC)protein,CNGC15,and the nitrate transceptor(NRT1.1)constitute a molecular switch that controls calcium influx depending on nitrate levels.The expression of CNGC15 is induced by nitrate,and its protein is localized at the plasma membrane after establishment of young seedlings.We found that disruption of CNGC15 results in the loss of the nitrate-induced Ca^(2+) signature(primary nitrate response)and retards root growth,reminiscent of the phenotype observed in the nrt1.1 mutant.We further showed that CNGC15 is an active Ca^(2+)-permeable channel that physically interacts with the NRT1.1 protein in the plasma membrane.Importantly,we discovered that CNGC15-NRT1.1 interaction silences the channel activity of the heterocomplex,which dissociates upon a rise in nitrate levels,leading to reactivation of the CNGC15 channel.The dynamic interactions between CNGC15 and NRT1.1 therefore control the channel activity and Ca^(2+) influx in a nitrate-dependent manner.Our study reveals a new nutrient-sensing mechanism that utilizes a nutrient transceptor-channel complex assembly to couple nutrient status to a specific Ca^(2+) signature. | Xiaohan Wang Changxin Feng LiLi Tian Congcong Hou Wang Tian Bin Hu Qian Zhang Zhijie Ren Qi Niu Jiali Song Dongdong Kong Liangyu Liu Yikun He Ligeng Ma Chengcai Chu Sheng Luan Legong Li | 2021 | Molecular Plant2021，14，5： | 5 |
| 3 | Cloning of two plastid division ftsZ genes from Nicotiana tabacum and their expression in E. coli显示文摘Two cDNAs of plastid division gene NtFtsZ1-1 and NtFtsZ1-2 are isolated from Nicotiana tabacum by RT-PCR and rapid amplification cDNA ends (RACE) method. Analysis of the deduced amino acid sequences encoded by NtFtsZ1-1 and NtFtsZ1-2 indicate that these two proteins possess the typical conservative motifs and GTP binding sites existing in all FtsZ proteins. The existence of putative plastid transit peptide in their N-terminal suggests that there are at least two transit-peptide containing FtsZ proteins in higher plants. Phylogenetic analysis based on amino acid sequences of FtsZ proteins also supports this interference. These two NtFtsZ genes demonstrate a similar expression pattern during the plant development, detected by Northern blot. Expression of NtFtsZ1-1 and NtFtsZ1-2 in E.coli interrupts the normal division process of host cells. These results suggest the diverse functions of FtsZ proteins in higher plants. | WANG Dong KONG Dongdong HU Yong JU Chuanli HE Yikun and SUN Jingsan(1. Department of Biology, Capital Normal University, Beijing 100037, China 2. Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China 3. College of Life Sciences, Beijing Normal University, Beijing 100875, China) | 2002 | Progress in Natural Science:Materials International2002，12，12： | 4 |
| 4 | Targeted deletion of mouse Rad1 leads to deficient cellular DNA damage responses显示文摘The Rad1 gene is evolutionarily conserved from yeast to human.The fission yeast Schizosaccharomyces pombe Rad1 ortholog promotes cell survival against DNA damage and is required for G_(2)/M checkpoint activation.In this study,mouse embryonic stem(ES)cells with a targeted deletion of Mrad1,the mouse ortholog of this gene,were created to evaluate its function in mammalian cells.Mrad1^(−/−)ES cells were highly sensitive to ultraviolet-light(UV light),hydroxyurea(HU)and gamma rays,and were defective in G_(2)/M as well as S/M checkpoints.These data indicate that Mrad1 is required for repairing DNA lesions induced by UV-light,HU and gamma rays,and for mediating G_(2)/M and S/M checkpoint controls.We further demonstrated that Mrad1 plays an important role in homologous recombination repair(HRR)in ES cells,but a minor HRR role in differentiated mouse cells. | Chunbo Zhang Yuheng Liu Zhishang Hu Lili An Yikun He Haiying Hang | 2011 | Protein & Cell2011，2，5： | 3 |
| 5 | Alginate encapsulated mesoporous silica nanospheres as a sustained drug delivery system for the poorly water-soluble drug indomethacin显示文摘We applied a combination of inorganic mesoporous silica material,frequently used as drug carriers,and a natural organic polymer alginate(ALG),to establish a sustained drug delivery system for the poorly water-soluble drug Indomethacin(IND).Mesoporous silica nanospheres(MSNs)were synthesized using an organic template method and then functionalized with aminopropyl groups through postsynthesis.After drug loading into the pores of aninopropyl functionalized MSNs(AP-MSNs),IND loaded AP-MSNs(IND-AP-MSNs)were encapsulated by ALG through the ionic interaction.The effects of surface chemical groups and ALG layer on IND release were systematically studied using scanning electron microscopy(SEM),transmission electron microscopy(TEM),nitrogen adsorption,zetapotential analysis and TGA analysis.The surface structure and surface charge changes of the ALG encapsulated AP-MSNs(ALG-AP-MSNs)were also investigated.The results showed that sustained release of IND from the designed drug delivery system was mainly due to the blockage effect from the coated ALG.We believe that this combination will help designing oral sustained drug delivery systems for poorly water-soluble drugs. | Liang Hu Changshan Sun Aihua Song Di Chang Xin Zheng Yikun Gao Tongying Jiang Siling Wang | 2014 | Asian Journal of Pharmaceutical Sciences2014，9，4： | 2 |
| 6 | ftsZ gene and plastid division显示文摘Plastid is one of the most important cellularorganelles, the normal division process of plastid is essential for the differentiation and development of plant cells. For a long time, morphological observations and genetic analyses to special mutants are the major research fields of plastiddivision, but the molecular mechanisms underlying plastiddivision are largely unknown. Because of the endosymbiotic origin, plastid division might have mechanisms in common with those involved in bacterial cell division. It has beenproved that several prokaryotic cell division genes also par-ticipate in the plastid division. Recently, the mechanisms of prokaryotic cell division have been well documented, which provides a valuable paradigm for understanding the plastid division mechanisms. In plants, the functional analyses of ftsZ, a key gene involved both in bacteria and plastid division, have established the solid foundation for people to under-stand the plastid division in molecular level. In this paper we will make a review for the research history and progress of plastid division. | KONG Dongdong WANG Dong HU Yong JU Chuanli WANG Yingdian HE Yikun SUN Jingsan | 2003 | Chinese Science Bulletin2003，48，12： | 1 |
| 7 | Multi-Panel Extra-Large Scale MIMO Based Joint Activity Detection and Channel Estimation for Near-Field Massive IoT Access显示文摘The extra-large scale multiple-input multiple-output(XL-MIMO)for the beyond fifth/sixth generation mobile communications is a promising technology to provide Tbps data transmission and stable access service.However,the extremely large antenna array aperture arouses the channel near-field effect,resulting in the deteriorated data rate and other challenges in the practice communication systems.Meanwhile,multi-panel MIMO technology has attracted extensive attention due to its flexible configuration,low hardware cost,and wider coverage.By combining the XL-MIMO and multi-panel array structure,we construct multi-panel XL-MIMO and apply it to massive Internet of Things(IoT)access.First,we model the multi-panel XL-MIMO-based near-field channels for massive IoT access scenarios,where the electromagnetic waves corresponding to different panels have different angles of arrival/departure(AoAs/AoDs).Then,by exploiting the sparsity of the near-field massive IoT access channels,we formulate a compressed sensing based joint active user detection(AUD)and channel estimation(CE)problem which is solved by AMP-EM-MMV algorithm.The simulation results exhibit the superiority of the AMP-EM-MMV based joint AUD and CE scheme over the baseline algorithms. | Zhen Gao Hanlin Xiu Yikun Mei Anwen Liao Malong Ke Chun Hu Mohamed-Slim Alouini | 2023 | China Communications2023，20，5： | 1 |
| 8 | An empirical study of security issues in SSO server-side implementations显示文摘Dear editor, Single sign-on (SSO) schemes have been widely used by major companies to manage service authorization and user authentication. They can enable third-party applications to obtain user information from a service provider to identify a user. | Hui WANG Dawu GU Yuanyuan ZHANG Yikun HU | 2022 | Science China(Information Sciences)2022，65，7： | 0 |
| 9 | Cis-regulatory element-based genome-wide identification of DREB1/CBF targets in Arabidopsis显示文摘Microarray analysis is used to identify transcriptional targets. However, the direct targets of transcription factors cannot be distin- guished from indirect ones; further, genes with low-expression levels cannot be identi ed by this method. In the present study, we exploit the cis-element dehydration-responsive element (DRE) that is known to be responsible for the transcription of DRE binding factor 1 (DREB1) targets in the promoter region of all Arabidopsis genes. Putative targets whose promoters contain the elements were veri ed by both microarray and reverse transcription-polymerase chain reaction (RT-PCR) analysis. Five new DREB1/CBF direct targets were identi ed. Compared with traditional microarray analysis, our method is convenient and cost-e ective for identifying the downstream targets of transcription factors. | Huan Zhao Weizhong Liu Yong Hu Xianglin Liu Yikun He | 2008 | Progress in Natural Science:Materials International2008，18，5： | 0 |
| 10 | Transfer of a eubacteria-type cell division site-determining factor CrMinD gene to the nucleus from the chloroplast genome in Chlamydomonas reinhardtii显示文摘MinD is a ubiquitous ATPase that plays a crucial role in selection of the division site in eubacteria, chloroplasts, and probably Archaea. In four green algae, Mesostigma viride, Nephroselmis olivacea, Chlorella vulgaris and Prototheca wickerhamii, MinD homologues are encoded in the plastid genome. However, in Arabidopsis, MinD is a nucleus-encoded, chloroplast-targeted protein involved in chloro- plast division, which suggests that MinD has been transferred to the nucleus in higher land plants. Yet the lateral gene transfer (LGT) of MinD from plastid to nucleus during plastid evolution remains poorly understood. Here, we identified a nucleus-encoded MinD homologue from unicellular green alga Chlamydomonas reinhardtii, a basal species in the green plant lineage. Overexpression of CrMinD in wild type E. coli inhibited cell division and resulted in the filamentous cell formation, clearly demon- strated the conservation of the MinD protein during the evolution of photosynthetic eukaryotes. The transient expression of CrMinD-egfp confirmed the role of CrMinD protein in the regulation of plastid division. Searching all the published plastid genomic sequences of land plants, no MinD homologues were found, which suggests that the transfer of MinD from plastid to nucleus might have occurred be- fore the evolution of land plants. | LIU WeiZhong HU Yong ZHANG RunJie ZHOU WeiWei ZHU JiaYing LIU XiangLin HE YiKun | 2007 | Chinese Science Bulletin2007，52，18： | 0 |
| 11 | Customizing the HPL for China accelerator显示文摘HPL is a Linpack benchmark package widely used in high-performance computing tests. Customizing the HPL is crucial for a heterogeneous system equipped with CPU and the China accelerator because of the complexity of the China accelerator and the specified interface on matrix multiplication built in the China accelerator. Therefore, it is advisable to use delicate partition and encapsulation on matrix(DPEM) to expose a friendly testing configuration. More importantly, we propose the orchestrating algorithm for matrix multiplication(OAMM) to enhance the efficiency of the heterogeneous system composed of CPU and China accelerator. Furthermore, optimization at vectorization(OPTVEC) is applied to shield the architectural details of the vector processing element(VPE) equipped in the China accelerator. The experimental results validate DPEM, OPTVEC and OAMM. OPTVEC optimizations would speed up matrix multiplication more than twofold, moreover OAMM would improve productivity by up to 10% compared to the traditional HPL tested in a heterogeneous system. | Xinbiao GAN Yikun HU Jie LIU Lihua CHI Han XU Chunye GONG Shengguo LI Yihui YAN | 2018 | Science China(Information Sciences)2018，61，4： | 0 |
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