3篇
您的检索式：作者名="Yincong Gu"
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| 1 | Whole-genome sequencing reveals rare off-target mutations in CRISPR/Cas9-edited grapevine显示文摘The CRISPR(clustered regularly interspaced short palindromic repeats)-associated protein 9(Cas9)system is a powerful tool for targeted genome editing,with applications that include plant biotechnology and functional genomics research.However,the specificity of Cas9 targeting is poorly investigated in many plant species,including fruit trees.To assess the off-target mutation rate in grapevine(Vitis vinifera),we performed whole-genome sequencing(WGS)of seven Cas9-edited grapevine plants in which one of two genes was targeted by CRISPR/Cas9 and three wild-type(WT)plants.In total,we identified between 202,008 and 272,397 single nucleotide polymorphisms(SNPs)and between 26,391 and 55,414 insertions/deletions(indels)in the seven Cas9-edited grapevine plants compared with the three WT plants.Subsequently,3272 potential off-target sites were selected for further analysis.Only one off-target indel mutation was identified from the WGS data and validated by Sanger sequencing.In addition,we found 243 newly generated off-target sites caused by genetic variants between the Thompson Seedless cultivar and the grape reference genome(PN40024)but no true off-target mutations.In conclusion,we observed high specificity of CRISPR/Cas9 for genome editing of grapevine. | Xianhang Wang Mingxing Tu Ya Wang Wuchen Yin Yu Zhang Hongsong Wu Yincong Gu Zhi Li Zhumei Xi Xiping Wang | 2021 | Horticulture Research2021，8，1： | 1 |
| 2 | Resequencing and genome-wide association studies of autotetraploid potato显示文摘Potato is the fourth most important food crop in the world.Although with a long history for breeding approaches,genomic information and association between genes and agronomic traits remain largely unknown particularly in autotetraploid potato cultivars,which limit the molecular breeding progression.By resequencing the genome of 108 main cultivar potato accessions with rich genetic diversity and population structure from International Potato Center,with approximate 20-fold coverage,we revealed more than 27 million Single Nucleotide Polymorphisms and~3 million Insertion and Deletions with high quality and accuracy.Domestication analysis and genome-wide association studies(GWAS)identified candidate loci related to photoperiodic flowering time and temperature sensitivity as well as disease resistance,providing informative insights into the selection and domestication of cultivar potato.In addition,GWAS with GWASploy for 25 agronomic traits identified candidate loci by association signals,especially those related to tuber size,small-sized tuber weight and tuber thickness that was also validated by transcriptome analysis.Our study provides a valuable resource that facilitates the elucidation of domestication process as well as the genetic studies and agronomic improvement of autotetraploid potato. | Feng Zhang Li Qu Yincong Gu Zhi-Hong Xu Hong-Wei Xue | 2022 | Molecular Horticulture2022，2，1： | 0 |
| 3 | DNA methylation modification in heterosis initiation through analyzing rice hybrid contemporary seeds显示文摘Heterosis is an important biological phenomenon and widely applied in agriculture.Although many studies have been performed by using vegetative organs of F_(1) hybrid plants,how heterosis (or hybrid vigor) is initiated and formed,particularly the underlying molecular mechanism,remain elusive.Hybrid contemporary seeds of rice indica varieties 9311 and PA64 were innovatively used and analysis of DNA methylome of embryo and endosperm at early developing stages revealed the globally decreased DNA methylation.Genes,especially those relate to hormones function and transcriptional regulation present non-additive methylation.Previously identified heterosis-related superior genes are non-additively methylated in early developing hybrid contemporary seeds,suggesting that key genes/loci responsible for heterosis are epigenetically modified even in early developing hybrid seeds and hypomethylation of hybrid seeds after cross-pollination finally result in the long-term transcriptional change of F_(1) hybrid vegetative tissues after germination,demonstrating that altered DNA methylation in hybrid seeds is essential for initiation regulation and maintenance of heterosis exhibiting in F_(1) hybrid plants.Notably,a large number of genes show non-additive methylation in the endosperm of reciprocal hybrids,suggesting that endosperm might also contribute to heterosis. | Shirong Zhou Meiqing Xing Zhilong Zhao Yincong Gu Yunping Xiao Qiaoquan Liu Hongwei Xue | 2021 | The Crop Journal2021，9，5： | 0 |
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