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| 1 | Comparative epidemiology of gastric cancer between Japan and China显示文摘AIM:To clarify the similarities and differences in gastric cancer epidemiology between Japan and China.METHODS:A comprehensive literature search of the PubMed database was performed.The relevant literature published in China was also been cited.Data on incidence and mortality rates in 2008 were obtained from the Cancer Mondial database,published by International Agency for Research on Cancer at http://gffzzdff0249c86de468fh5b9f6k9nv56x6u96.ffgz.tsg.suse.edu.cn/.RESULTS:Gastric cancer remains a significant publichealth burden in both Japan and China.The prevalence of Helicobacter pylori(H.pylori)colonization is high in the adult populations of both countries.Accumulating evidence from intervention studies in both countries has shown the effectiveness of H.pylori eradication in reduc-ing gastric cancer incidence.There are differences,however,in many aspects of gastric cancer,including patterns of incidence and mortality,trends in the prevalence of H.pylori infection,H.pylori strains,the magnitude of risk of gastric cancer related to H.pylori infection,and associations with dietary habits.Compared with China,Japan has seen a more rapid decline in H.pylori infection among adolescents.While Japanese cohort studies have dominated the literature concerning the associations between gastric cancer and dietary habits,numerous case-control studies in China suggest a positive association between a high intake of preserved fish and vegetables and gastric cancer risk.There is a need for a multidisciplinary research approach to understand the interactions between various strains of H.pylori,host factors,and other lifestyle and environmental factors in gastric carcinogenesis in both countries.CONCLUSION:The shared high incidence of gastric cancer and high prevalence of H.pylori,as well as differences in many aspects of gastric cancer,provide an excellent opportunity to establish Sino-Japanese collaborations. | Yingsong Lin Junko Ueda Shogo Kikuchi Yukari Totsuka Wen-Qiang wei You-Lin Qiao Manami Inoue | 2011 | World Journal of Gastroenterology2011，17，39： | 64 |
| 2 | Diallyl disulfide-induced G2/M arrest of human gastric cancer MGC803 cells involves activation of p38 MAP kinase pathways显示文摘AIM: To determine the role of p38 MAP kinase signal transduction pathways in diallyl disulfide (DADS)-induced G2/M arrest in human gastric cancer MGC803 cells.METHODS: MGC803 cell growth inhibition was measured by MTT assay. Phase distribution of cell cyde was analyzed by flow cytometry. Expression of Cdc25C, p38, phosphorylation of p38 (pp38) were determined by Western blotting.RESULTS: MT1- assay showed that SB203580, a specific p38 MAPK inhibitor blocked DADS-induced growth inhibition.Flow cytometry analysis revealed that treatment of MGC803 cells with 30 mg/L DADS increased the percentage of cells in the G2/M phase from 9.3% to 39.4% (P<0.05), whereas inhibition of p38 activity by SB203580 abolished induction of G2/M arrest by DADS. Western blotting showed that phosphorylation of p38 was increased 3.52-fold following treatment of MGC803 cells with 30 mg/L DADS for 20 min(P<0.05), whereas Cdc25C was decreased 68% following treatment of MGCS03 cells with 30 mg/L DADS for 24 h(P<0.05). Decreased Cdc25C protein expression by DADS was attenuated by SB203580 (P<0.05).CONCLUSION: DADS-induced G2/M arrest of MGC803 cells involves activation of p38 MAP kinase pathways.Decreased Cdc25C protein expression by p38 MAPK played a crucial role in G2/M arrest after treatment with DADS. | Jing-PingYuan Gui-HuaWang HuiLing QiSu Yue-HongYang YingSong Rong-JunTang YaoLiu ChenHuang | 2004 | World Journal of Gastroenterology2004，10，18： | 25 |
| 3 | Thinning and Shrinkage of Laohugou No. 12 Glacier in the Western Qilian Mountains, China, from 1957 to 2007显示文摘Landsat images, real-time kinematic GPS measurements, and topographic maps were used to determine changes in ice elevation, volume, and areal extent of the Laohugou No. 12 glacier (Qilian Mountains, China) between 1957 and 2007. The glacier experienced significant thinning and areal shrinkage in the ablation zone, but slight thickening in part of the accumulation zone. Elevation decreased by 18.6±5.4 m between 1957 and 2007 in the regions covered by the GPS measurements. The total volume loss for the entire glacier was estimated to be 0.218 km3 using a third-order polynomial fit method. The area diminished by 0.28 km2 between 1957 and 1994, 0.26 km2 between 1994 and 2000, and 0.28 km2 between 2000 and 2007, suggesting that the rate of loss in glacial coverage has increased since the mid-1990s. Significant increases in annual mean air temperature may have contributed to shrinkage and thinning of the glacier. | ZHANG Yingsong LIU Shiyin SHANGGUAN Donghui LI Jing ZHAO Jingdong | 2012 | Journal of Mountain Science2012，9，3： | 16 |
| 4 | Mutation of RET gene in Chinese patients with Hirschsprung's disease显示文摘AIM: To investigate the pathogenic mechanism of Hirschsprung's disease (HD) at the molecular level and to elucidate the relationship between RET oncogene and Chinese patients with HD.METHODS: Exon 13 of RET oncogene from 20 unrelated HD patients was analyzed with polymerase chain reactionsingle strand conformation polymorphism (PCR-SSCP). The positive amplifying products were then sequenced. According to the results of SSCP and DNA sequence, SSCP was done as well for the samples from the family other members of some cases with mutated RET gene.RESULTS: SSCP analysis indicated that mobility abnormality existed in 4 unrelated HD patients. Direct DNA sequence analysis identified a missense mutation, T to G at the nucleotide 18 888 and a frameshift mutation at the nucleotide 18 926 insG. In a HD family, the sicked child and his father were the same heterozygous missense mutation (T to G at nucleotide 18 888).CONCLUSION: Among Chinese HD patients, RET gene mutations may exist in considerable proportion with different patterns. These new discoveries indicate that RET mutations may play an important role in the pathogenesis of unrelated HD in the Chinese population. PCR-SSCP combined with DNA sequence can be used as a tool in the genetic diagnosis of HD. | Ji-ChengLi Shi-PingDing YingSong Min-JuLi | 2002 | World Journal of Gastroenterology2002，8，6： | 7 |
| 5 | Viral and Antibody Kinetics of COVID-19 Patients with Different Disease Severities in Acute and Convalescent Phases:A 6-Month Follow-Up Study显示文摘Coronavirus disease 2019(COVID-19),caused by severe acute respiratory syndrome coronavirus 2(SARS-CoV-2),has spread rapidly around the world,posing a major threat to human health and the economy.Currently,long-term data on viral shedding and the serum antibody responses in COVID-19 patients are still limited.Herein,we report the clinical features,viral RNA loads,and serum antibody levels in a cohort of 112 COVID-19 patients admitted to the Honghu People’s Hospital,Hubei Province,China.Overall,5.36%(6/112)of patients showed persistent viral RNA shedding(>45 days).The peak viral load was higher in the severe disease group than in the mild group(median cycle threshold value,36.4 versus 31.5;P=0.002).For most patients the disappearance of IgM antibodies occurred approximately 4–6 weeks after symptoms onset,while IgG persisted for over 194 days after the onset of symptoms,although patients showed a 46%reduction in antibodies titres against SARS-CoV-2 nucleocapsid protein compared with the acute phase.We also studied18 asymptomatic individuals with RT-qPCR confirmed SARS-CoV-2 infection together with 17 symptomatic patients,and the asymptomatic individuals were the close contacts of these symptomatic cases.Delayed IgG seroconversion and lower IgM seropositive rates were observed in asymptomatic individuals.These data indicate that higher viral loads and stronger antibody responses are related to more severe disease status in patients with SARS-CoV-2 infection,and the antibodies persisted in the recovered patient for more than 6 months so that the vaccine may provide protection against SARS-CoV-2 infection. | Xiaoyong Zhang Suwen Lu Hui Li Yi Wang Zhen Lu Zhihong Liu Qingtao Lai Yali Ji Xuan Huang Yongyin Li Jian Sun Yingsong Wu Xiaoning Xu Jinlin Hou | 2020 | Virologica Sinica2020，35，6： | 4 |
| 6 | Aeroelastic stability analysis of heated panel with aerodynamic loading on both surfaces显示文摘Focusing on the aeroelastic stability of thin panel structure of airframe component such as engine nozzle of high-speed flight vehicles,a nonlinear aeroelastic model for a two-dimensional heated panel exposing both surfaces to the airflow with different aerodynamic pressures is established.The von Karman large deflection plate theory and the first-order piston theory are used in the formulation of aeroelastic motion.The critical conditions for aeroelastic stability and the stability boundaries are obtained using theoretical analysis and numerical computations,respectively.The results show that the panel is more prone to become unstable when its two surfaces are subject to aerodynamic loading simultaneously;only if the sum of the aerodynamic pressures on both surfaces of the panel satisfies flutter stability condition,can the panel be likely aeroelastically stable;compared with the general panel flutter problem that only one surface is exposed to the airflows,the present condition makes the panel become aeroelastically unstable at relatively small flight aerodynamic pressure. | ZHOU Jian YANG ZhiChun GU YingSong | 2012 | Science China(Technological Sciences)2012，55，10： | 3 |
| 7 | CONSTRUCTION OF HU-PBL/SCID CHIMERAS AND DEVELOPMENTOF EBV-RELATED LYMPHOMAS显示文摘Objective To construct hu-PBL/SCID chimeras and to investigate the development of lymphoma and oncogenicity of the Epstein-Barr virus (EBV). Methods Human peripheral blood lymphocytes (PBLs) were isolated from healthy adult donors and transplanted intraperitoneally into severe combined immunodeficient(SCID) mice. Mice with hu-PBL engraftment from healthy EBV seronegative donors were injected intraperitoneally with EBV-containing supernatant from suspension culture of B95-8 cell line (active infection), whereas mice receiving lymphocytes from healthy EBV seropositive donors were not re-infected with B95-8 derived EBV (latent infection). Pathological examination and molecular analysis were performed on experime-ntal animals and induced neoplasms. Results In the early stage of this experiment, 12 mice died of acute graft-versus-host disease, mortality was 34.3% (12/35 mice) with an average life span of 17.5 days. In 19 survival hu-PBL/SCID chimeric recipients from 12 healthy donors, tumor incidence was 84.2% (16/19 mice). The average survival time of tumor-bearing mice was 65.5 days. EBV-related neoplasms in SCID mice were nodular tumors with aggressive and fatal features. Histological morphology of tumors exhibited diffuse large cell lymphomas. Immunohistochemistry revealed that LCA (CD45) and L26 (CD20) were positive, but both PS1 (CD3) and UCHL-1 (CD45RO) were negative, and EBV products ZEBRA, LMP1, and EBNA2 were expressed in a small number of tumor cells. EB virus particles were seen in the nuclei of some tumor cells by electron microscopy, and EBV DNA could be amplified in the tumor tissues by PCR. In situ hybridization indicated that the nuclei of tumor cells contained human-specific Alu sequence. Conclusions EBV-induced tumors were human B-cell malignant lymphomas. We obtained direct causative evidence dealing with EBV-associated tumor deriving from normal human cells. | Run-liangGan KeLan Zhi-huaYin Li-jiangWang YingSong Kai-taiYao | 2005 | Chinese Medical Sciences Journal2005，20，1： | 3 |
| 8 | Robust flutter analysis based on genetic algorithm显示文摘Robust flutter analysis considering model uncertain parameters is very important in theory and engineering applications.Modern robust flutter solution based on structured singular value subject to real parametric uncertainties may become difficult because the discontinuity and increasing complexity in real mu analysis.It is crucial to solve the worst-case flutter speed accurately and efficiently for real parametric uncertainties.In this paper,robust flutter analysis is formulated as a nonlinear programming problem.With proper nonlinear programming technique and classical flutter analysis method,the worst-case parametric perturbations and the robust flutter solution will be captured by optimization approach.In the derived nonlinear programming problem,the parametric uncertainties are taken as design variables bounded with perturbed intervals,while the flutter speed is selected as the objective function.This model is optimized by the genetic algorithm with promising global optimum performance.The present approach avoids calculating purely real mu and makes robust flutter analysis a plain job.It is illustrated by a special test case that the robust flutter results coincide well with the exhaustive method.It is also demonstrated that the present method can solve the match-point robust flutter solution under constant Mach number accurately and efficiently.This method is implemented in problem with more uncertain parameters and asymmetric perturbation interval. | GU YingSong ZHANG XinPing YANG ZhiChun | 2012 | Science China(Technological Sciences)2012，55，9： | 2 |
| 9 | Monosialoganglioside protected ischemic rat hippocampal slices through stabilizing expression of N-methyl-D-aspartate receptor subunit显示文摘AIM: To determine direct protective effect of monosialoganglioside (GM1) on hippocampal slices after oxygen- glucose deprivation and reperfusion (OGD/RP), and investigate the influence on the expression of N-methyl-D- aspartate receptor subunit 1 (NMDAR1) in those hippocampal slices. METHODS: Injury of hippocampal slices and protective effects of GM1 were detected by 2,3,5-triphenyltetrazolium chloride (TTC) staining, toluidine blue staining, and transmission electron microscopy of rat hippocampal slices. Expression of NMDAR1 was detected by Western blot. RESULTS: (1) GM1 at 1.0 μmol/L was the most effective concentration to preserve the TTC staining of the hippocampal slices after OGD/RP (P<0.05), and the next was GM1 at 10.0 μmol/L(P<0.05). (2) Toluidine blue staining and transmission electron microscopy showed GM1 protected the injuried hippocamal slices after OGD/RP. (3) GM1 downregulated the temporally high expression of NMDAR1 in the hippocampal slices immediately after a 25-min OGD and prevented the over low expression of NMDAR1 after a 30-min reperfusion. CONCLUSION: GM1 could protect injuried rat hippocampal slices after OGD/RP through stabilizing the expres- sion of NMDAR1. | Jian-renLIU Mei-pingDING Er-qingWEI Jian-zhengHUANG YingSONG QiangDING Qiu-fuGE | 2004 | Acta Pharmacologica Sinica2004，25，6： | 2 |
| 10 | Nationwide epidemiological survey of chronic pancreatitis in Japan显示文摘 | Yingsong Lin Akiko Tamakoshi Seiki Matsuno Kazunori Takeda Tetsuo Hayakawa Motoji Kitagawa Satoru Naruse Takashi Kawamura Kenji Wakai Rie Aoki Masayo Kojima Yoshiyuki Ohno | 2000 | Journal of Gastroenterology2000，，2： | 2 |
| 11 | Construction and expression of nucleic acid vaccine pVAX1-h-alpha S_(1-140) coding human alpha-synuclein显示文摘BACKGROUND: The deposition of α-synuclein (α-syn) aggregates is a neuropathological feature of Parkinson’s disease. It remains impossible to involve α-syn aggregation in the treatment of Parkinson’s disease. A nucleic acid vaccine will provide a new pathway to immunotherapy for Parkinson’s disease. OBJECTIVE: To construct a recombinant eukaryotic expression vector pVAX1 coding human α-syn and to observe its expression level in COS-7 cells. DESIGN AND SETTING: The present bioengineering and molecular biology experiment was performed at Department of Neurology, First Affiliated Hospital of Chongqing Medical University & Chongqing Key Laboratory of Neurology. MATERIALS: The eukaryotic expression plasmid pVAX1, human embryonic brain tissue, healthy human blood cells, and COS-7 cells were purchased from Promega Company, USA. METHODS: The full-length CDS sequence of the human α-syn gene was amplified by RT-PCR, which contained restriction sites for the enzymes KpnⅠ, XbaⅠand Kozak consensus sequence. Then the PCR products and eukaryotic expression vector pVAX1 were digested with KpnⅠand XbaⅠsimultaneously, and were extracted and ligated by T4 ligase. The recombinant constructs pVAX1-hα-S1-140 were transformed into competent E. coli TOP 10 cells and the positive clones were screened and selected using PCR analysis, restriction digestion analysis, and DNA sequencing. The constructs were then tested for protein expression in COS-7 cells by RT-PCR and Western blotting. MAIN OUTCOME MEASURES: Identification of an eukaryotic expression vector containing the human α-syn gene, pVAX1-hα-S1-140, and detection of the expression in mammalian cell COS-7. RESULTS: The pVAX1 vector was successfully cloned with humanα-syn in the correct orientation and in-frame. The DNA vaccine constructs pVAX1-hα-S1-140 with the humanα-syn gene were shown to be expressed in COS-7 cells. Humanα-syn was successfully expressed in the mammalian cell line and was detected by RT-PCR and western blotting. CONCLUSION: Nucleic acid vaccine pVAX1-hα S1-140 was successfully constructed and expressed in COS-7 cells. | Jiacai Wang Yingsong Ouyang Shaojun Wang Guoguang Peng Qin Luo Side Jiang FaxiangWang | 2008 | Neural Regeneration Research2008，3，12： | 2 |
| 12 | ABO blood type, long-standing diabetes, and the risk of pancreatic cancer显示文摘AIM: To retrospectively study pancreatic cancer patients with respect to their ABO blood type and diabetes. METHODS: Our analysis included a cohort of 1017 patients with pancreatic ductal cancer diagnosed at our hospital in Tokyo. They were divided into two groups: 114 patients with long-standing type 2 diabetes (DM group, defined as diabetes lasting for at least three years before the diagnosis of pancreatic cancer) and 903 patients without diabetes (non-DM group). Multivariate analysis was performed to identify factors that are associated with long-standing diabetes. The DM group was further divided into three subgroups according to the duration of diabetes (3-5 years, 5.1-14.9 years, and 15 years or more) and univariate analyses were performed. RESULTS: Of the 883 pancreatic cancer patients with serologically assessed ABO blood type, 217 (24.6%) had blood type O. Compared with the non-DM group, the DM group had a higher frequency of blood type B [odds ratio (OR) = 2.61, 95%CI: 1.24-5.47; reference group: blood type A]. Moreover, male (OR = 3.17, 95%CI: 1.67-6.06), older than 70 years of age (OR = 2.19, 95%CI: 1.20-3.98) and presence of a family history of diabetes (OR = 6.21, 95%CI: 3.38-11.36) were associated with long-standing type 2 diabetes. The mean ages were 64.8 ± 9.2 years, 67.1 ± 9.8 years, and 71.7 ± 7.0 years in the subgroups with the duration of diabetes, 3-5 years, 5.1-14.9 years, and 15 years or more, respectively (P = 0.007). A comparison of ABO blood type distribution among the subgroups also showed a significant difference (P = 0.03). CONCLUSION: The association of pancreatic cancer with blood type and duration of diabetes needs to be further examined in prospective studies. | Naoto Egawa Yingsong Lin Taku Tabata Sawako Kuruma Seiichi Hara Ken Kubota Terumi Kamisawa | 2013 | World Journal of Gastroenterology2013，19，16： | 2 |
| 13 | Compact coplanar waveguide fed ultra wideband antenna with a notch band characteristic显示文摘 | LI Yingsong YANG Xiaodong | 2011 | AEU-Intemational Journal of Electron- ics and Communications2011，65，11： | 1 |
| 14 | Deformation of PDMS membrane and mierocantilever by a water droplet: comparison between Mooney-Rivlin and linear elastic constitutive models 显示文摘 | YU Yingsong ZHAO Yapu | 2009 | Journal of Colloid and Inter- face Science2009，332，2： | 1 |
| 15 | Dual-band antenna han- dles WLAN/WiMAX 显示文摘 | LI Yingsong YANG Xiaodong | 2011 | Microwaves & RF2011，50，6： | 1 |
| 16 | Compact coplanar waveguide fed ultra wideband antenna with a notch band characteristic 显示文摘 | LI Yingsong YANG Xiaodong | 2011 | AEU-Intemational Jouranal of Elec- tronics and Communications2011，65，11： | 1 |
| 17 | Tiny UWB an- tenna notches two bands 显示文摘 | LI Yingsong YANG Xiaodong BAI Yu | 2012 | Microwaves & RF2012，51，2： | 1 |
| 18 | A cognitive radio antenna integrated with narrow/ultra-wideband antenna and switches 显示文摘 | LI Yingsong LI Wenxing MITFRA R | 2012 | IEICE Electronics Express2012，9，15： | 1 |
| 19 | Implementation and Investigation of a compact circular wide slot UWB an- tenna with dual notched band characteristics using stepped impedance resonators 显示文摘 | LI Yingsong LI Wenxing MITYRA R | 2012 | IEICE Electronics Express2012，21，1： | 1 |
| 20 | Nutritional factors and risk of pancreatic cancer: a population-based case-control study based on direct interview in Japan显示文摘 | Yingsong Lin Akiko Tamakoshi Tetsuo Hayakawa Satoru Naruse Motoji Kitagawa Yoshiyuki Ohno | 2005 | Journal of Gastroenterology2005，，3： | 1 |
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