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| 1 | Combining the first class with second class to improve the professional skills of medical students显示文摘Medical science is a highly practical subject,which is closely related to human life.Medical institutions take a heavy social responsibility,in addition to the sacred mission of teaching and educating.The professional skills of medical students are one important assessment indicators to become a qualified doctor.Therefore,how to improve the professional skills of medical students in their training process becomes crucial. | Ni Yanbo Ji Piyou Wang Chunyan Zhang Lixia Shao Yiye Luan Xiying | 2013 | 重庆医学2013，42，23： | 1 |
| 2 | Analysis and countermeasures of the conflicting between clinical practice and taking test of postgraduate of medical undergraduates显示文摘Nowadays,with the expanding enrollment of medical schools in our country,the competitive pressures of employment increase.It is very difficult for the graduates of these schools to find ideal jobs,but medical students with master or doctor degrees are relatively optimistic to the employment situation.As a result,more and more fresh medical graduates pursue the post-graduate education,thus forms a'rush of taking test of postgraduate'phenomenon[1].Clinical students | Zhang Lixia Gu Yun Cai Heng Liang Shaohua Shao Yiye Ni Yanbo | 2014 | 重庆医学2014，43，25： | 1 |
| 3 | Evaluation of P-glycoprotein-targeting circulating microRNAs as peripheral biomarkers for medically intractable epilepsy显示文摘Background Early diagnosis of medically intractable epilepsy is challenging in clinical work.P-glycoprotein(P-gp)is one of the most important multidrug efux transporters,which has been demonstrated to contribute to the drug resistance of intractable epilepsy.The present study was aimed to explore the diagnostic value of microRNAs(miRNAs)targeting P-gp for medically intractable epilepsy.Methods Thirty-six patients with intractable epilepsy and 36 epilepsy patients responsive to anti-epilepsy drugs,who visited Jinshan Hospital of Fudan University from September 2014 to September 2016,were enrolled in this study.Clinical information of the patients was obtained by retrospectively reviewing medical records.MiRNAs with diferential serum expression between the two groups of patients were detected by microarray assay.Meanwhile,miRNAs that were confrmed to regulate P-gp in vitro by western blot were selected for further validation.In the validation phase,reverse transcription quantitative PCR(RT-qPCR)was conducted to confrm the diferential expression of the candidate miRNAs in the epilepsy cohorts.Receiver operating characteristic(ROC)curve analysis was carried out to evaluate the diagnostic value of the miRNAs for intractable epilepsy.Results Three miRNAs including miR-6514-3p,miR-6076-5p,and miR-6855-3p were identifed to be candidate miRNAs by microarray assay.The results of western blotting validated that miR-146a-5p and miR-138-5p could regulate P-gp expression in vitro,so they were included in the candidate miRNAs for further validation.In the validation phase,the results of RT-qPCR indicated that compared with drug-responsive patients,the patients with intractable epilepsy showed decreased level of miR-138-5p and increased level of miR-146a-5p.The results of ROC curve analysis indicated that miR-138-5p(AUC=0.877)and miR-146a-5p(AUC=0.866)had high diagnostic value for intractable epilepsy.In addition,the miR-panel composed of miR-138-5p and miR-146a-5p showed higher diagnostic value(AUC=0.926)than the miRNAs selected by microarray assay.Conclusions Our results indicated that the dysregulated miR-138-5p and miR-146a-5p which target P-gp expression have high potential as peripheral biomarkers for medically intractable epilepsy. | Yangmei Xie Yiye Shao Xue Gong Ming Wang Yinghui Chen | 2023 | Acta Epileptologica2023，6，1： | 0 |
| 4 | Long non-coding RNA Tug 1 regulates inflammation in microglia and in status epilepticus rats through the NF-κB signaling pathway显示文摘Background:Inflammation plays an important role in the pathogenesis of status epilepticus(SE).The long non-cod-ing RNA(lncRNA)taurine up-regulated gene1(Tug1)plays a well-defned role in inflammatory diseases.However,the molecular mechanism of Tug1 in SE progression remains unknown.In present study,we investigated whether Tug1 is involved in microglial inflammation in SE rats.Methods:The SE rat model was established via intraperitoneal injection of lithium chloride pilocarpine.RNA-binding protein immunoprecipitation(RIP)and RIP sequencing were carried out in rat microglia(RM).Tug1 cloned into the adenovirus was overexpressed in the microglia.Knockdown of Tug1 was performed via siRNA transfection.The level of Tug1 and inflammatory factors IL-1βand TNF-αwas examined by real-time polymerase chain reaction(RT-PCR)and western blotting.Protein levels of p65,P-p65,p-IKBa and IkBa were assessed by western blotting.Results:The RIP-seq result showed 14 lncRNAs that bound to the NF-κB p65 protein in RM.The lncRNA Tug1 directly interacted with p65.The level of declined Tug1 was decreased in the hippocampus of SE rats.Overexpression ofTug1 reduced the LPS-induced inflammation and M1/M2 polarization of microglia,while knockdown of Tug1 aggravated the inflammatory response in microglia.Accordingly,the protein levels of p-p65/p65 and p-IkBa/IkBa were reduced in the Tug1-overexpression microglia and elevated in the Tug1-knockdown microglia.Conclusions:These findings indicate that Tug1 modulates the inflammation in microglia through the NF-κB signal pathway,and the Tug1/P65 axis are like to play a signifcant role in the inflammatory processes,providing a valid target for the therapy of SE. | Ming Wang Yangmei Xie Yiye Shao Yinghui Chen | 2022 | Acta Epileptologica2022，5，2： | 0 |
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