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| 1 | Dendritic Cells Transduced with SOCS1 Gene Exhibit Regulatory DC Properties and Prolong Allograft Survival显示文摘SOCS1 is a key regulator of cytokine signaling and is important for maintaining balance in the immune system. It is thought to participate in negative feedback loops in cytokine signaling and may be an important signal for the regulation of dendritic cell (DC) maturation. However, it remains unclear whether DCs transduced with SOCS1 exhibit characteristics of regulatory DCs and induce allogeneic T-cell hyporesponsiveness. In this study, we constructed adenoviral vector coding SOCS1 (Ad-SOCS1) that can efficiently increase SOCS1 gene expression in bone marrow-derived dendritic cells. DCs transduced with Ad-SOCS1 (DC-SOCS1) expressed low levels of costimulatory and MHC molecules, were resistant to maturation and activation stimulation, induced allogeneic T-cell hyporesponsiveness, and promoted the generation of regulatory-like T cells in vitro. DC-SOCS1 pretreatment significantly prolonged the survival of allografts and led to a substantial increase in the generation of regulatory T cells. Our data suggest that SOCS1 inhibits DC maturation and induces regulatory DC generation, therefore possessing therapeutic potential to prevent rejection in organ transplantation. | Hong Fu Shaohua Song Fang Liu Zhijia Ni Xiaoyun Shen Liang Xiao Guoshan Ding Quanxing Wang | 2009 | Cellular & Molecular Immunology2009，6，2： | 20 |
| 2 | Types,Characteristics and Significances of Migrating Pathways of Gas-bearing Fluids in the Shenhu Area,Northern Continental Slope of the South China Sea显示文摘The first marine gas hydrate expedition in China has been conducted by Guangzhou Marine Geological Survey in the Shenhu Area, northern continental slope of the South China Sea. Previous study has analyzed the P-T conditions, geophysical anomalies and saturation calculations of these gas hydrates, but has not documented in detail the migration of gas-bearing fluids in the study area. Based on the interpretations of 2D/3D seismic data, this work identified two types of migration pathways for gas-bearing fluids in the Shenhu area, i.e., vertical and lateral pathways. The vertical pathways(largescale faults, gas chimneys and mud diapirs) presented as steep seismic reflection anomalies, which could be traced downward to the Eocene source rocks and may penetrate into the Late Miocene strata. The deeper gases/fluids might be allowed migrating into the shallower strata through these vertical conduits. However, the distributions showed distinct differences between these pathways. Large-scale faults developed only in the north and northeast of the Shenhu area, while in the drilling area gas chimneys were the sole vertical migration pathways. Since the Pliocene, normal faults, detachment faults and favorable sediments have constituted the lateral pathways in the Shenhu gas hydrate drilling area. Although these lateral pathways were connected with gas chimneys, they exerted different effects on hydrate formation and accumulation. Gas-bearing fluids migrated upward along gas chimneys might further migrate laterally because of the normal faults, thereby enlarging the range of the chimneys. Linking gas chimneys with the seafloor, the detachment faults might act as conduits for escaping gases/fluids. Re-deposited sediments developed at the early stage of the Quaternary were located within the gas hydrate stability zone, so hydrates would be enriched in these favorable sediments. Compared with the migration pathways(large-scale faults and mud diapirs) in the LW3-1 deep-sea oil/gas field, the migration efficiency of the vertical pathways(composed of gas chimneys) in the gas hydrate drilling area might be relatively low. Description and qualitative discrimination of migration pathways in the Shenhu gas hydrate drilling area are helpful to further understand the relationship between good-quality deep source rocks and shallow, mainly biogenicallyproduced, hydrates. As the main source rocks of the Baiyun sag, lacustrine mudstones in the Wenchang and Enping Formations may provide thermogenic methane. Gas chimneys with relatively low migration efficiency created the vertical pathways. Caused by the Dongsha tectonic movement, the release of overpressured fluids might reduce the vertical migration rates of the thermogenic methane. The thick bathyal/abyssal fine-grained sediments since the Late Miocene provided migration media with low permeability. These preconditions may cause carbon isotopic fractionation ofthermogenic methane during long-distance vertical migrations. Therefore, although geochemical analyses indicate that the methane forming gas hydrate in the Shenhu area was mainly produced biogenically, or was mixed methane primarily of microbial origin, thermogenic methane still contribute significantly. | SU Ming SHA Zhibin ZHANG Cuimei WANG Hongbin WU Nengyou YANG Rui LIANG Jinqiang QIAO Shaohua CONG Xiaorong LIU Jie | 2017 | Acta Geologica Sinica(English Edition)2017，91，1： | 16 |
| 3 | Finite element investigation of the poroelastic effect on the Xinfengjiang Reservoir-triggered earthquake显示文摘Coulomb failure stress changes (ΔCFS) are used in the study of reservoir-induced seismicity (RIS) generation.The threshold value of ΔCFS that can trigger earthquakes is an important issue that deserves thorough research.The M s 6.1 earthquake in the Xinfengjiang Reservoir in 1962 is well acknowledged as the largest reservoir-induced earthquake in China.Therefore, it is a logical site for quantitative calculation of ΔCFS induced by the filling of the reservoir and for investigating the magnitude of CFS that can trigger reservoir seismic activities.To better understand the RIS mechanism, a three-dimensional poroelastic finite element model of the Xinfengjiang Reservoir is proposed here, taking into consideration of the precise topography and dynamic water level.We calculate the instant changes of stress and pore pressure induced by water load, and the time variation of effective stresses due to pore water diffusion.The CFS on the seismogenesis faults and the accumulation of strain energy in the reservoir region are also calculated.Primary results suggest that the reservoir impoundment increases both pore pressure and CFS on the fault at the focal depth.The diffusion of pore pressure was likely the main factor that triggered the main earthquake, whereas the elastic stress owing to water load was relatively small.The magnitude of CFS on seismogenesis fault can reach approximately 10 kPa, and the ΔCFS values at the hypocenter can be about 0.7-3.0 kPa, depending on the fault diffusion coefficient.The calculated maximum vertical subsidence caused by the water load in the Xinfengjiang Reservoir is 17.5 mm, which is in good agreement with the observed value of 15 mm.The accumulated strain energy owing to water load was only about 7.3×10 11 J, even less than 1% of the seismic wave energy released by the earthquake.The reservoir impoundment was the only factor that triggered the earthquake. | CHENG HuiHong ZHANG Huai ZHU BoJing SUN YuJun ZHENG Liang YANG ShaoHua SHI YaoLin | 2012 | Science China Earth Sciences2012，55，12： | 11 |
| 4 | 3D printing of silk fibroin-based hybrid scaffold treated with platelet rich plasma for bone tissue engineering显示文摘3D printing/bioprinting are promising techniques to fabricate scaffolds with well controlled and patient-specific structures and architectures for bone tissue engineering.In this study,we developed a composite bioink consisting of silk fibroin(SF),gelatin(GEL),hyaluronic acid(HA),and tricalcium phosphate(TCP)and 3D bioprinted the silk fibroin-based hybrid scaffolds.The 3D bioprinted scaffolds with dual crosslinking were further treated with human platelet-rich plasma(PRP)to generate PRP coated scaffolds.Live/Dead and MTT assays demonstrated that PRP treatment could obviously promote the cell growth and proliferation of human adipose derived mesenchymal stem cells(HADMSC).In addition,the treatment of PRP did not significantly affect alkaline phosphatase(ALP)activity and expression,but significantly upregulated the gene expression levels of late osteogenic markers.This study demonstrated that the 3D printing of silk fibroin-based hybrid scaffolds,in combination with PRP post-treatment,might be a more efficient strategy to promote osteogenic differentiation of adult stem cells and has significant potential to be used for bone tissue engineering. | Liang Wei Shaohua Wu Mitchell Kuss Xiping Jiang Runjun Sun Reid Patrick Xiaohong Qin Duan Bin | 2019 | Bioactive Materials2019，4，1： | 6 |
| 5 | Optimizing the CRISPR/Cas9 system for genome editing in grape by using grape promoters显示文摘The efficacy of the CRISPR/Cas9 system in grapevine(Vitis vinifera L.)has been documented,but the optimization of this system,as well as CRISPR/Cas9-mediated multiplex genome editing,has not been explored in this species.Herein,we identified four VvU3 and VvU6 promoters and two ubiquitin(UBQ)promoters in grapevine and demonstrated that the use of the identified VvU3/U6 and UBQ2 promoters could significantly increase the editing efficiency in grape by improving the expression of sgRNA and Cas9,respectively.Furthermore,we conducted multiplex genome editing using the optimized CRISPR/Cas9 vector that contained the conventional multiple sgRNA expression cassettes or the polycistronic tRNA-sgRNA cassette(PTG)by targeting the sugar-related tonoplastic monosaccharide transporter(TMT)family members TMT1 and TMT2,and the overall editing efficiencies were higher than 10%.The simultaneous editing of TMT1 and TMT2 resulted in reduced sugar levels,which indicated the role of these two genes in sugar accumulation in grapes.Moreover,the activities of the VvU3,VvU6,and UBQ2 promoters in tobacco genome editing were demonstrated by editing the phytoene desaturase(PDS)gene in Nicotiana benthamiana leaves.Our study provides materials for the optimization of the CRISPR/Cas9 system.To our knowledge,our simultaneous editing of the grape TMT family genes TMT1 and TMT2 constitutes the first example of multiplex genome editing in grape.The multiplex editing systems described in this manuscript expand the toolbox of grape genome editing,which would facilitate basic research and molecular breeding in grapevine. | Chong Ren Yanfei Liu Yuchen Guo Wei Duan Peige Fan Shaohua Li Zhenchang Liang | 2021 | Horticulture Research2021，8，1： | 6 |
| 6 | Preparation of DHA-and EPA-enriched Glycerides by Enzymatic Interesterification Using Tuna Oil and Capric Acid显示文摘This study focused on the preparation of docosahexaenoic acid(DHA) and eicosapentaenoic acid(EPA)enriched-triacylglycerols by enzymatic interesterification using tuna oil and capric acid. The content of DHA+EPA is 26.86% in the tuna oil used in this study. A response surface methodology(RSM) was used to optimize the reaction parameters(reaction temperature, substrate molar ratio, enzyme amount and reaction time), and the optimized conditions were determined to be: reaction temperature 58℃, substrate molar ratio(capric acid : tuna oil) 4:1, enzyme amount 4%, and reaction time 7.5 h. Under the optimized conditions, the content of DHA+EPA in the glycerides was 40.03%, which is 13.17% higher than that in raw tuna oil. In addition,the MLM-type structured lipids containing medium chain fatty acids(capric acid) at positions sn-1,3 and a long chain fatty acid(DHA/EPA) at the position sn-2 may have many health benefits for humans. | LIANG Shaohua WANG Mengyuan ZHU Lijie ZHANG Man YANG Ming | 2018 | Grain & Oil Science and Technology2018，1，2： | 4 |
| 7 | A key‘foxy’aroma gene is regulated by homologyinduced promoter indels in the iconic juice grape‘Concord’显示文摘‘Concord’,the most well-known juice grape with a parentage of the North American grape species Vitis labrusca L.,possesses a special‘foxy’aroma predominantly resulted from the accumulation of methyl anthranilate(MA)in berries.This aroma,however,is often perceived as an undesirable attribute by wine consumers and rarely noticeable in the common table and wine grape species V.vinifera.Here we discovered homology-induced promoter indels as a major genetic mechanism for species-specific regulation of a key‘foxy’aroma gene,anthraniloyl-CoA:methanol acyltransferase(AMAT),that is responsible for MA biosynthesis.We found the absence of a 426-bp and/or a 42-bp sequence in AMAT promoters highly associated with high levels of AMAT expression and MA accumulation in‘Concord’and other V.labrusca-derived grapes.These promoter variants,all with direct and inverted repeats,were further confirmed in more than 1,300 Vitis germplasm.Moreover,functional impact of these indels was validated in transgenic Arabidopsis.Superimposed on the promoter regulation,large structural changes including exonic insertion of a retrotransposon were present at the AMAT locus in some V.vinifera grapes.Elucidation of the AMAT genetic regulation advances our understanding of the‘foxy’aroma trait and makes it genetically trackable and amenable in grapevine breeding. | Yingzhen Yang JoséCuenca Nian Wang Zhenchang Liang Honghe Sun Benjamin Gutierrez Xiaojun Xi Jie Arro YiWang Peige Fan Jason Londo Peter Cousins Shaohua Li Zhangjun Fei Gan-Yuan Zhong | 2020 | Horticulture Research2020，7，1： | 3 |
| 8 | Small molecule SMU-CX24 targeting toll-like receptor 3 counteracts inflammation:A novel approach to atherosclerosis therapy显示文摘Toll-like receptor 3(TLR3),as an important pattern recognition receptor(PRR),dominates the innate and adaptive immunity regulating many acute and chronic inflammatory diseases.Atherosclerosis is proved as an inflammatory disease,and inflammatory events involved in the entire process of initiation and deterioration.However,the contribution of TLR3 to atherosclerosis remains unclear.Herein,we identified the clinical relevance of TLR3 upregulation and disease processes in human atherosclerosis.Besides,activation of TLR3 also directly led to significant expression of atherogenic chemokines and adhesion molecules.Conversely,silencing TLR3 inhibited the uptake of oxLDL by macrophages and significantly reduced foam cell formation.Given the aberrance in TLR3 functions on atherosclerosis progression,we hypothesized that TLR3 could serve as novel target for clinical atherosclerosis therapy.Therefore,we developed the novel ellipticine derivative SMU-CX24,which specifically inhibited TLR3(IC_(50)=18.87±2.21 nmol/L).In vivo,atherosclerotic burden was alleviated in Western diet fed ApoE^(−/−)mice in response to SMU-CX24 treatment,accompanying notable reductions in TLR3 expression and inflammation infiltration within atherosclerotic lesion.Thus,for the first time,we revealed that pharmacological downregulation of TLR3 with specific inhibitor regenerated inflammatory environment to counteract atherosclerosis progression,thereby proposing a new strategy and probe for atherosclerosis therapy. | Xiaohong Cen Baoqu Wang Yuqing Liang Yanlin Chen Yu Xiao Shaohua Du Kutty Selva Nandakumar Hang Yin Shuwen Liu Kui Cheng | 2022 | Acta Pharmaceutica Sinica B2022，12，9： | 2 |
| 9 | Study on liquids diffusion into and relevant corrosion behaviour of glass fibre reinforced polymer used in high voltage composite insulator显示文摘Long-term liquids diffusion tests were performed on glass fibre reinforced polymer(GFRP)used in high voltage composite insulator.The plate-shaped GFRP specimens were immersed in three types of liquids,which high voltage composite insulator would encounter in practice,including deionised water,NaCl solution and nitric acid solution for a period of 4000 h.Chemical reaction and physical dissolution complicated the diffusion processes,which could be divided into three types based on the features of diffusion curves.Fourier transform infrared spectroscopy,scanning electron microscopy and optical interferometry were employed to investigate the chemical property,two-dimensional(2D)and 3D surface morphologies of GFRP.The results revealed that the surface layer of GFRP was destroyed to some extent depending on the type and concentration of the liquid,the maximum of profile height deviation of GFRP surface was proposed to evaluate the extent of the corrosion of GFRP,the greater the profile height deviation,the more serious the corrosion of GFRP.The present work is helpful for a better understanding of the diffusion behaviour of liquids into GFRP used in high voltage composite insulator as well as in other industry applications. | Yanfeng Gao Xidong Liang Weining Bao Chao Wu Shaohua Li Yingyan Liu Yuanji Cai | 2020 | High Voltage2020，5，1： | 2 |
| 10 | Identification of Re-Deposited Sediments and the Relationships with Heterogeneous Distributions of Gas Hydrates in the Shenhu Area显示文摘The first marine gas hydrate drilling expedition(GMGS-1)of China was performed in the Shenhu Area,northern slope of the South China Sea(Fig.1a)by Guangzhou Marine Geological Survey(GMGS)during April to June,2007,to detect the occurrences of gas hydrates.The drilling results showed that the distributions of hydrates in the drilling area were heterogeneous.In | SU Ming SHA Zhibin QIAO Shaohua LIANG Jinqiang LIU Jie YANG Rui WU Nengyou | 2015 | Acta Geologica Sinica(English Edition)2015，89，A01： | 2 |
| 11 | Realizing the air brazing of ZrO_(2) ceramics through Al metal显示文摘The exploration towards cost-effective filler metal for ceramics joining has always been the key issues for ceramics joining.Herein,we reveal that the Al metal prefers to spread on the ZrO_(2) based ceramic under the air heating condition,due to the geometric limit effects by in-situ formed dense Al2O3 surface.Inspired by this,the joining of ZrO_(2) based ceramics was realized in air with Al metal as filler,through the diffusion of Al towards ceramic side.The Al element can induce obvious interfacial bonding effect on Al2O3 layer and ZrO_(2) ceramic,where the hybridization among the Al-p,Zr-d and O-p orbitals plays a key role.The in-situ formed Al2O3 layer on Al filler surface is vital for forming the fine interface(shear strength of~36 MPa),which results in the relief of lattice mismatch and peak stress at ceramic-filler metal transition interface. | Yaotian Yan Baishen Liu Tianxiong Xu Liang Qiao Shaohua Qin Jian Cao Junlei Qi | 2022 | Journal of Materiomics2022，8，3： | 2 |
| 12 | Practice and exploration of educational innovation in the teaching process of anatomy显示文摘In the advent of knowledge economy and information society,the society urgently needs talents with high-quality,creativity and comprehensive development.School education plays an extremely important role in educating creative innovation. | Liu Hongfu Liang Shaohua Zhang Luping Zhao Dongmei Huang Fei | 2012 | 重庆医学2012，41，25： | 2 |
| 13 | Cytotoxicity and binding profiles of activated CrylAc and Cry2Ab to three insect cell lines显示文摘 | Jizhen Wei Gemei Liang Kongming Wu Shaohua Gu Yuyuan Guo Xinzhi Ni Xianchun Li | 2018 | Insect Science2018，25，4： | 2 |
| 14 | Chemical Characteristics and Fatty Acid Profile of Foxtail Millet Bran Oil显示文摘 | Shaohua Liang Guolong Yang Yuxiang Ma | 2010 | Journal of the American Oil Chemists’ Society2010，，1： | 2 |
| 15 | Research Advances in Interplay of Bursaphelenchus xylophilus and Other Key Biological Factors Related to Pine Wilt Disease显示文摘The paper separately discusses interplay of pine wood nematode and several key biological factors related to pine wilt disease, such as host trees, insect vectors, fungi, symbiotic bacteria, and natural enemy acarid, etc. By virtue of so much complicated interplay among the biological factors related to pine wilt disease which is different from other forest disease, it also points out that the further researches about pine wilt disease should focus on interplay mechanism of key biological factors to discover pathogenic mechanism, and simple and quick inspection and quarantine methods. | LIU Huimin NING Shaohua LIANG Jun LU Quan | 2006 | Chinese Forestry Science and Technology2006，5，3： | 1 |
| 16 | Chemical Characteristics and Fatty Acid Profile of Foxtail Millet Bran Oil显示文摘 | Shaohua Liang Guolong Yang Yuxiang Ma | 2010 | Journal of the American Oil Chemists’ Society2010，，1： | 1 |
| 17 | Multiscale analysis of Asian Monsoon over the past 640 ka显示文摘The empirical mode decomposition(EMD) method is used to re-analyse the high-resolution and precisely-dated stalagmite record from Chinese caves over the past 640 ka. Results show that(1) the variation in the Asian Monsoon can be completely decomposed into ten quasiperiod oscillations, among which the precession and semiprecession band oscillations are the most prominent periodicities, with contribution rates of 31.1% and 30.7%, respectively;(2) the cross-spectrum analysis of the semiprecession component and bi-hemisphere insolation(BHI) are strongly correlated, indicating an amplified response of precipitation and temperature variability to the interhemispheric insolation in the low-latitude regions, thus further affecting the intensity of the Asian Monsoon;(3) on millennial timescales, obvious oscillations at the 5 ka and 1–2 ka bands roughly correspond to the classical Bond and Dansgaard-Oeschger(DO) cycles. Additionally, a strong correlation is found between the detrended stalagmite δ18 O records and Ca/Sr sequence from the North Atlantic(especially at the 5 ka band). This result means that the 5 ka cycle is characteristic of the glacial-interglacial cycle since the middle and late Pleistocene and may imply that climate change on the millennial timescale is the result of an interaction between global ice volume and insolation. | Yalan ZENG Shitao CHEN Shaohua YANG Yijia LIANG Yongjin WANG | 2019 | Science China Earth Sciences2019，62，5： | 1 |
| 18 | Determining the contributions of urbanisation and climate change to NPP variations over the last decade in the Yangtze River Delta, China显示文摘 | Shaohua Wu Shenglu Zhou Dongxiang Chen Zongqiang Wei Liang Dai Xingong Li | 2014 | Science of the Total Environment2014，，： | 1 |
| 19 | Chemical characteristics and fatty acid profile of foxtail millet bran oil 显示文摘 | LIANG Shaohua YANG Guolong MA Yuxiang | 2010 | J Am Oil Chem Soc2010，87，： | 1 |
| 20 | Volatiles of grape berries evaluated at the germplasm level by headspace-SPME with GC–MS显示文摘 | Chunxiang Yang Yiju Wang Zhenchang Liang Peige Fan Benhong Wu Liu Yang Younian Wang Shaohua Li | 2008 | Food Chemistry2008，，3： | 1 |
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