|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Improvement of oxytetracycline production mediated via cooperation of resistance genes in Streptomyces rimosus显示文摘Increasing the self-resistance levels of Streptomyces is an effective strategy to improve the production of antibiotics.To increase the oxytetracycline(OTC) production in Streptomyces rimosus,we investigated the cooperative effect of three co-overexpressing OTC resistance genes:one gene encodes a ribosomal protection protein(otrA) and the other two express efflux proteins(otrB and otrC).Results indicated that combinational overexpression of otrA,otrB,and otrC(MKABC) exerted a synergetic effect.OTC production increased by 179%in the recombinant strain compared with that of the wild-type strain M4018.The resistance level to OTC was increased by approximately two-fold relative to the parental strain,thereby indicating that applying the cooperative effect of self-resistance genes is useful to improve OTC production.Furthermore,the previously identified cluster-situated activator OtcR was overexpressed in MKABC in constructing the recombinant strain MKRABC;such strain can produce OTC of approximately7.49 g L^((-1)),which represents an increase of 19%in comparison with that of the OtcR-overexpressing strain alone.Our work showed that the cooperative overexpression of self-resistance genes is a promising strategy to enhance the antibiotics production in Streptomyces. | Shouliang Yin Xuefeng Wang Mingxin Shi Fang Yuan Huizhuan Wang Xiaole Jia Fang Yuan Jinliang Sun Tiejun Liu Keqian Yang Yuxiu Zhang Keqiang Fan Zilong Li | 2017 | Science China(Life Sciences)2017，60，9： | 10 |
| 2 | Molecular mechanism of dehydrin in response to environmental stress in plant显示文摘Dehydrins,近来作为 D-11 亚群知道胚胎开始丰富(草地) 蛋白质,是免疫蛋白质的逻辑地不同的家庭,典型地响应象干旱那样的各种各样的环境压力在干燥容忍的种子胚胎或在植物的纸巾积累,咸度并且结冰。保守序列的存在指定了为 K, 5,并且 Y 片断是在所有 dehydrins 发现的片断代表的 dehydrins,和 K 的一个结构的特征一高度保存了 15 氨基酸主题(EKKGIMDKIKEKLPG ) 和形式一个 amphiphilic 螺旋。根据这些领域和聚类的分析的安排, dehydrins 被细分进 5 种子类型:YnSK, Kn, KnS, SKn 和 YnK。dehydrins 的不同类型被不同环境应力在植物导致。学习结果证明 dehydrins 可能经由很多不同机制在不能生活的应力下面起重要保护的作用,包括改进或保护由在对 freez/thaw 或脱水作出回应的 cryoprotective 活动的酶活动;作为膜 stabilizer 由函数稳定泡或另外的 endomembrane 结构在期间结冰导致了脱水,并且阻止膜系统氧化损坏作为激进的 scavenger 由反应的氧激进分子导致了。这里,基因表示和响应在植物的应力的 dehydrin 的分子的机制被讨论。 | ZHANG Yuxiu WANG Zi XU Jin | 2007 | Progress in Natural Science:Materials International2007，17，3： | 9 |
| 3 | The localization of type 2 diabetes susceptibility gene loci in northern Chinese Han families显示文摘We conducted a genome-wide scan, in which 358 well distributed fluorescent dye-labeled microsatellite marker sets were applied in 32 Chinese Han type 2 diabetes families from Northern China to search for the susceptibility gene loci. The data collected from screening all the chromosomes of genome were genotyped by using genescan and genotyping software, then, parametric and non-parametric multipoint test, and affected sib-pair analysis as well, were used to analyze the data. We identified some susceptibility gene loci residing in chromosomes 1,12,18,20, respectively, or precisely, located around D1S214, D1S207, D1S218, D1S235, D12S336, D18S61 and D20S118. The comparison of this result with those from other regions and races reflected the complexity and heterogeneity of type 2 diabetes. | ZHAO Jinying WANG Heng XIONG Momiao HUANG Wei ZUO Jin CHEN Zhu QIANG Boqin SUN Qi LI Yuxiu LIU Qiuying DU Weinan CHEN Jialun DING Wei YUAN Wentao ZHAO Yang XU Hongyan JIN Li FANG Fude | 2000 | Chinese Science Bulletin2000，45，19： | 8 |
| 4 | Development of a new method for analyzing free aluminum ions(Al^(3+)) in seafood using HPLC-ICP-MS显示文摘This paper presents a novel method for simultaneous online examination of free aluminum ions(Al3+)in seafood,using solidphase extraction and high-performance liquid chromatography online with inductively coupled plasma mass spectrometry(SPEHPLC-ICP-MS),without post-column reaction.The optimum conditions for chromatographic separation of Al3+were achieved using an IonPac CS5A analytical column with an IonPac CG5A guard column.The mobile phase consisted of 0.040 mol/L LiOH,0.0060 mol/L 2,6-pyridinedicarboxylic acid,and 0.090 mol/L CH3COOH(pH 4.7).The free Al3+ions in seafood were extracted by shaking with the mobile phase at 70°C for 2 h.SPE was conducted using an Oasis MCX,3cc/60 mg,30μm column,which was activated and equilibrated with 2 mL of methanol and 4 mL of deionized water before use.HCl(0.075 mol/L,2 mL)was used to wash inorganic Al from the SPE column.The standard recoveries of Al3+were all above 89%and the relative standard deviations were all below 5%.The proposed method was successfully used for the examination of Al3+in seafood samples,and the results were similar to those obtained using the static equilibrium method. | SHANG DeRong ZHAO YanFang ZHAI YuXiu NING JinSong DING HaiYan SHENG XiaoFeng | 2013 | Chinese Science Bulletin2013，58，35： | 8 |
| 5 | EFFECTS OF LEAD WIDTHS AND PITCHES ON RELIABILITY OF QUAD FLAT PACKAGE(QFP)SOLDERED JOINTS显示文摘有限元素方法(女性) 被用来在焊接关节的可靠性上分析铅宽度和程度的效果。为 QFP 设备的最佳模拟也被研究。结果显示当铅程度是一样时,焊接关节的最大的相等的压力与增加铅宽度增加,当焊接关节的可靠性减少时。当铅宽度是一样时,焊接关节的最大的相等的压力不与增加铅程度完全减少,最大的相等的压力价值的最小的价值在所有曲线存在。在这个条件下面,焊接关节的最大的相等的压力是相对最不,焊接关节的可靠性高,集会是优秀的。模仿的结果显示最好的参数:铅宽度是 0.2 公里,铅沥青是 0.3 公里(在二领先之间的距离是 0.1 公里) ,它现在为 QFP 设备的 micromation 被有益于。当铅宽度是 0.25 公里,铅沥青是 0.35 公里时,焊接关节的最大的相等的压力的最小的值存在(在二领先之间的距离是 0.1 公里) ,设备能很长时间服务,可靠性最高,汇编是优秀的。模仿的结果也显示铅宽度是 0.15 公里和铅沥青的事实也许是 0.2 公里 QFP 的限制,它为高级的铅计数和汇编的 micromation 是重要的。 | XUE Songbai WU Yuxiu HAN Zongjie WANG Jianxin | 2007 | Chinese Journal of Mechanical Engineering2007，20，4： | 7 |
| 6 | Assessment and Comparison of in vitro Immunoregulatory Activity of Three Astaxanthin Stereoisomers显示文摘In recent years,the immune-modulatory role of all-trans astaxanthin from different pigment sources has been studied.It was reported that all-trans astaxanthin might exist as three stereoisomers,and the composition of all-trans stereoisomers in natural materials differs from that of synthetic products.However,the different biological effects of various all-trans stereoisomers still remain unclear.In the present study,we evaluated the bioactivity of three astaxanthin stereoisomers,(3S,3'S)-trans-,(3R,3'R)-transand meso-trans-astaxanthin,in regulating cell-mediated immune response using mice lymphocytes and peritoneal exudates cells(PECs) systems.After the treatment with three astaxanthin stereoisomers(20 μmol L-1),the lymphocyte proliferation capacity,neutral red phagocytosis of PECs and natural killer(NK) cell cytotoxic activity were comparatively assessed.The results showed that all three astaxanthin stereoisomers significantly promoted lymphocyte proliferation,phagocytic capacity of PECs,and cytotoxic activity of NK cells.Moreover,the(3S,3'S)-trans-astaxanthin exhibited a much higher response than others. | SUN Weihong XING Lihong LIN Hong LENG Kailiang ZHAI Yuxiu LIU Xiaofang | 2016 | Journal of Ocean University of China2016，15，2： | 7 |
| 7 | An experimental and numerical study of the freak wave speed显示文摘The propagation speed is one of the most important characteristics for describing freak waves. The research of freak wave speed is not only helpful for understanding the generation mechanism and evolution process of freak waves, but also applicable to the prediction. A stable and accurate method is proposed for the calculation of the freak wave speed, in which physical model tests are carried out to measure the motion of the largest wave crest along the wave tank. The linear regression relationship between the spatial position of the largest wave crest and instantaneous moment is established to calculate the speed of totally 248 cases of experimental freak waves and 312 supplementary cases of numerical freak waves. Based on the calculate results, a semitheoretical and semiempirical formula is proposed by using a regression analysis method to predict the speed of the freak wave, and the nonlinear characteristic of the freak wave speed is also investigated. | CUI Cheng ZHANG Ningchuan KANG Haigui YU Yuxiu | 2013 | Acta Oceanologica Sinica2013，32，5： | 7 |
| 8 | Effects of Sini San used alone and in combination with fluoxetine on central and peripheral 5-HT levels in a rat model of depression显示文摘OBJECTIVE: To investigate the effects of Sini San and fluoxetine on the levels of central and peripheral 5-HT in a rat model of depression, and provide new insight into the treatment of depression with integrated Chinese-Western Medicine.METHODS: A rat model of depression was established by chronic mild stress(CMS). Model rats received either Sini San, fluoxetine, a combination of the two drugs, or no drug treatment. Healthy naive rats were used as controls. Open field and sucrose preference tests were used to assess depression-like behavior. ELISA and immunohistochemistry were used to determine central and peripheral levels of 5-HT.RESULTS: In the group with no drug treatment,central 5-HT expression decreased while peripheral5-HT concentrations increased as CMS continued.Four weeks after CMS, Sini San alone was less effective in reducing depression-like behavior than fluoxetine alone or in combination with Sini San,but combined use was more effective than fluoxetine alone. Eight weeks after CMS, Sini San alone or in combination with fluoxetine was more effective in reducing depression-like behavior than fluoxetine alone. Furthermore Sini San and fluoxetine used alone or in combination notably increased central 5-HT expression and decreased peripheral 5-HT levels in the rat model.CONCLUSION:The results of the present study indicate that there is a synergistic action between the two medicines in the treatment of depression. Sini San exhibited a relatively long lag before its effects were observed; however, by eight weeks the Traditional Chinese Medicine appeared at least as effective as fluoxetine. We suggest that Sini San can replace fluoxetine in the later stages of depression treatment to minimize side effects observed with long-term fluoxetine administration. | Yubo Li Yuxiu Sun Xueling Ma Xiaoxing Xue Wenting Zhang Zhiqian Wu Yulin Ouyang Jianxin Chen Weiming Wang Shuzhen Guo Wei Wang | 2013 | Journal of Traditional Chinese Medicine2013，33，5： | 6 |
| 9 | Detection of phthalates migration from disposable tablewares to drinking water using hexafluoroisopropanol-induced catanionic surfactant coacervate extraction显示文摘Hexafluoroisopropanol(HFIP)-induced sodium dodecyl sulfate/dodecyltrimethylammonium bromide(SDS/DTAB) catanionic surfactant coacervate extraction method coupled with high performance liquid chromatography(HPLC) was used to detect the migration of phthalates from disposable tablewares to drinking water. The concentration factors are larger than 82 and extraction recoveries over 53% for water samples spiked with 100 or 200 ng/m L phthalates. Limit of detection is in the range of 1.0–2.6 ng/m L.Good linearity with correlation coefficients larger than 0.9985 is obtained in the concentration of20–1500 or 40–3000 ng/m L. Relative recoveries are from 82.4% to 123.6% for water samples spiked with30/60, 250/500, and 1500/3000 ng/m L phthalates, respectively. Relative standard deviations(RSDs) are0.4%–7.4% for intraday precision(n = 5) and 0.6%–7.8% for interday precision(n = 3). Four of studied phthalates are found in the drinking water samples prepared from four kinds of tablewares. | Cao Li Jia Xu Dan Chen Yuxiu Xiao | 2016 | Journal of Pharmaceutical Analysis2016，6，5： | 5 |
| 10 | Study on Measurement Methods of Multi-Directional Waves—Wave Gauge Array Method显示文摘- Measuring multi-directional waves with the wave gauge array is one of the fundamental and easily realised methods. In this paper, the wave gauge array is described and the effects of the gauge spacing, the array orientations, etc. of the three array arrangements, i. e., linear array, T-type array and pentagon array, on the resolution of the directional spreading of waves, are investigated experimentally. This study can be used as a reference in the experimental study and the field measurement of directional waves. | Yu Yuxiu Liu Shuxue Prof. Department of Civil Engineering, Dalian University of Technology, Dalian Assistant Researcher, Department of Civil Engineering, Dalian University of Technology, Dalian | 1992 | China Ocean Engineering1992，7，2： | 5 |
| 11 | Expression of hippocampal corticosteroid receptors,as well as corticotrophin-releasing hormone and vasopressin in the hypothalamic paraventricular nucleus,in fornix transected rats显示文摘BACKGROUND:The hippocampus regulates the hypothalamic-pituitary-adrenal axis through negative feedback.The hypothalamic paraventricular nucleus receives neuronal input from the hippocampus via the fornix. OBJECTIVE:To explore whether the negative feedback effect of the hippocampus on the hypothalamic-pituitary-adrenal axis is contributed to the inhibitory effect of mineralocorticoid receptor (MR) and glucocorticoid receptor(GR) in the hippocampus on the paraventricular nucleus via the fornix. DESIGN,TIME AND SETTING:Randomized,controlled,animal experiment.The study was performed at the Department of Histology and Embryology,China Medical University between September 2006 and September 2008. MATERIALS:Rabbit anti-rat anti-MR and rabbit anti-rat anti-GR antibodies were purchased from Santa Cruz Biotechnology,USA.Rabbit anti-rat anti-corticotrophin releasing hormone(CRH) and rabbit anti-rat anti-arginine vasopressin antibodies were purchased from Wuhan Boster. METHODS:A total of 90 male,Wistar rats were randomly divided into model and sham-surgery groups(n=45).Fornix transection was performed in the model group,while the sham-surgery group underwent surgery,but no fornix transection. MAIN OUTCOME MEASURES:Immunohistochemistry was used to examine MR and GR expression in the hippocampus,as well as CRH and anti-arginine vasopressin in the paraventricular nucleus.Western blot was used to measure alterations in MR,GR,and CRH protein expression following fomix transection. RESULTS:Compared with the sham-surgery group,there were no obvious changes in MR and GR expression in the hippocampus,or CRH and anti-arginine vasopressin expression in the paraventricular nucleus within 4 days of fornix transection.However,after 7-10 days,significantly decreased MR and GR expression in the hippocampus,and increased CRH and anti-arginine vasopressin expression in the paraventricular nucleus were observed(P<0.05-0.01). CONCLUSION:Negative feedback from the hippocampus on the hypothalamic-pituitary-adrenal axis might be mediated through the fornix,and the corticosterone actions mediated by hippocampal corticosteroid receptors indirectly modulated the hypothalamic-pituitary-adrenal axis. | Fang Han Hong Liu Yanhui Zhang Yuxiu Shi | 2009 | Neural Regeneration Research2009，4，5： | 4 |
| 12 | Three-dimensional numerical simulation of obfique wave action on vertical walls显示文摘A three-dimensional numerical model based on the potential theory was developed to study the oblique wave action on vertical walls. A source term inside the domain was used to generate incident waves and outgoing waves were dissipated by sponge layers and trans- mitted by radiation boundaries. The finite difference method was used to solve the governing equations and boundary conditions in the regular transformed domain in σ-coordinate. Satisfactory agreements between the numerical predictions and experimental results of wave force were obtained. It is concluded that the maximum wave force acting on the vertical walls is induced by the obliquely inci- dent waves rather than the normally incident waves. | LI Benxia YU Xiping YU Yuxiu | 2006 | Acta Oceanologica Sinica2006，25，1： | 4 |
| 13 | An Association Study on the Cognitive Function and the Cerebral Grey Matter Volume of Patients with First-Episode Schizophrenia显示文摘 | Xinyue ZHANG Jingjing YAO Yiding LV Xiaoxin ZHAO Yuan LI Yuxiu SUI Zhiping DAI | 2018 | 上海精神医学2018，30，3： | 4 |
| 14 | A nanocleaner specifically penetrates the blood-brain barrier at lesions to clean toxic proteins and regulate inflammation in Alzheimer's disease显示文摘Insurmountable blood-brain barrier(BBB) and complex pathological features are the key factors affecting the treatment of Alzheimer's disease(AD).Poor accumulation of drugs in lesion sites and undesired effectiveness of simply reducing Aβ deposition or TAU protein need to be resolved urgently.Herein,a nanocleaner is designed with a rapamycin-loaded ROS-responsive PLGA core and surface modification with KLVFF peptide and acid-cleavable DAG peptide [R@(ox-PLGA)-KcD].DAG can enhance the targeting and internalization effect of nanocleaner towards neurovascular unit endothelial cells in AD lesions,and subsequently detach from nanocleaner in response to acidic microenvironment of endosomes to promote the transcytosis of nanocleaner from endothelial cells into brain parenchyma.Then exposed KLVFF can capture and carry Aβ to microglia,attenuating Aβ-induced neurotoxicity.Strikingly,rapamycin,an autophagy promoter,is rapidly liberated from nanocleaner in the high ROS level of lesions to improve Aβ degradation and normalize inflammatory condition.This design altogether accelerates Aβ degradation and alleviates oxidative stress and excessive inflammatory response.Collectively,our finding offers a strategy to target the AD lesions precisely and multi-pronged therapies for clearing the toxic proteins and modulating lesion microenvironment,to achieve efficient AD therapy. | Ting Lei Zhihang Yang Xue Xia Yuxiu Chen Xiaotong Yang Rou Xie Fan Tong Xiaolin Wang Huile Gao | 2021 | Acta Pharmaceutica Sinica B2021，11，12： | 4 |
| 15 | Groupiness of Sea Waves and Their Characteristic Parameters显示文摘Three methods for studying wave groups and their main parameters for describing wave groupiness are reviewed in this paper. Then they are analyzed and compared combined with field data from both aspects of group height and group length. A method and two parameters that can describe wave groupiness are suggested. The groupiness parameters of sea waves at three field stations are given. The effects of groupiness on both distributions of the wave height and the phase of component waves are investigated. The effects of datum length on the calculated value of grouping parameters are also discussed. | Yu Yuxiu Gui Manhai Professor, Dept. of Civil Eng. Dalian University of Technology, Dalian 116024 Master, Dept. of Civil Eng. Dalian University of Technology, Dalian 116024 | 1996 | China Ocean Engineering1996，11，1： | 3 |
| 16 | Investigation of Ionic Polymer Metal Composite Actuators Loaded with Various Tetraethyl Orthosilicate Contents显示文摘 | Qingsong He Min Yu Yuxiu Li Yan Ding Dongjie Guo Zhendong Dai | 2012 | Journal of Bionic Engineering2012，9，1： | 3 |
| 17 | Design and evaluation of a novel transdermal patch containing diclofenac and teriflunomide for rheumatoid arthritis therapy显示文摘The aim of this study was to design a compound transdermal patch containing diclofenac(DA)and teriflunomide(TEF)for the treatment of rheumatoid arthritis(RA).The various organic amines salts of DA were prepared and their forming was confirmed using DSC and FTIR.The percutaneous permeation of organic amines salt of DA was investigated in vitro using a two-chamber diffusion cell with excised rabbit skin as transdermal barrier.The formulation of the patch was optimized in terms of the concentration of percutaneous permeation enhancer and the loading dose of drugs.The pharmacokinetic behavior of the optimal formulation was studies in rabbits and the anti-inflammatory and analgesic effects of the optimal patch were evaluated with the adjuvant arthritis model in rats and the pain model in mice,respectively.The result showed that skin penetration of diclofenactriethylamine(DA-TEtA)salt was better than other organic amine salts.Based on previous study of our laboratory,teriflunomide-triethylamine(TEF-TEtA)significantly enhanced the skin permeation of TEF.10%of azone(AZ)was the best enhancer for the two drugs.The optimal patch formulation was composed of 2%of TEF-TEtA,6%of DA-TEtA and 10%of AZ.The cumulative permeated amount of DA-TEtA in vitro was comparable with that of the commercial diclofenac-diethylamine(DA-DEtA)patch.The absolute bioavailability of TEFTEtA was 42%,which could achieve the therapeutic drug levels.In animal study,the optimized compound patch containing DA-TEtA and TEF-TEtA displayed significant antiinflammatory and analgesic effect,which indicated the potential of the compound patch. | Yuxiu Zhang Dongmei Cun Xin Kong Liang Fang | 2014 | Asian Journal of Pharmaceutical Sciences2014，9，5： | 3 |
| 18 | The grouping characteristics of sea waves in the Shijiu Port显示文摘A review concerning the methods of studying and describing wave groups is presented in this paper. After analysing 78 field records collected in the Shijiu Port, China, the measured parameters of wave groups and some factors describing wave groupness and their variations are given. Moreover, these results are compared with those of theory. | Yu Yuxiu and Liu Shuxue Dalian University of Technology, Dalian, 116024, China | 1991 | Acta Oceanologica Sinica1991，10，3： | 2 |
| 19 | Ultra-low dark count InGaAs/InP single photon avalanche diode显示文摘A low noise InGaAs/InP single photon avalanche diode(SPAD) is demonstrated. The device is based on planar type separate absorption, grading, charge and multiplication structure. Relying on reasonably designed device structure and low-damage Zn diffusion technology, excellent low-noise performance is achieved. Due to its importance, the physical mechanism of dark count is analyzed through performance characterization at different temperatures. The device can achieve 20% single photon detection efficiency and 320 Hz dark count rate(DCR) with a low after pulsing probability of 0.57% at 233 K. | LI Bin NIU Yuxiu FENG Yinde CHEN Xiaomei | 2022 | Optoelectronics Letters2022，18，11： | 2 |
| 20 | An efficient focusing model for generation of freak waves显示文摘Based on the Longuet-Higgins wave model theory, the previews studies have shown that freak waves can be generated in finite space and time successfully. However, as to generating high nonlinear freak waves, the simulation results will be unrealistic. Therefore, a modified phase modulation method for simulating high nonlinear freak waves was developed. The surface elevations of some wave components at certain time and place are positive by modulating the corresponding random initial phases, then the total surface elevation at the focused point is enhanced and furthermore a freak wave event is generated. The new method can not only make the freak wave occur at certain time and place, but also make the simulated wave surface time series satisfy statistical properties of the realistic sea state and keep identical with the target wave spectrum. This numerical approach is of good precision and high efficiency by the comparisons of the simulated freak waves and the recorded freak waves. | LIU Zanqiang ZHANG Ningchuan YU Yuxiu | 2011 | Acta Oceanologica Sinica2011，30，6： | 2 |
您所在的机构暂未开通此部分的功能，请联系管理员