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您的检索式：作者名="Yuanyue Shan"
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| 1 | ^(131)I-Evans blue: evaluation of necrosis targeting property and preliminary assessment of the mechanism in animal models显示文摘Necrosis is a form of cell death, which is related to various serious diseases such as cardiovascular disease, cancer, and neurodegeneration. Necrosis-avid agents(NAAs) selectively accumulated in the necrotic tissues can be used for imaging and/or therapy of related diseases. The aim of this study was to preliminarily investigate necrosis avidity of ^(131)I-evans blue( ^(131)I-EB) and its mechanism. The biodistribution of ^(131)I-EB at 24 h after intravenous administration showed that the radioactivity ratio of necrotic to viable tissue was 3.41 in the liver and 11.82 in the muscle as determined by γ counting in model rats. Autoradiography and histological staining displayed preferential uptake of ^(131)I-EB in necrotic tissues. In vitro nuclear extracts from necrotic cells exhibited 82.3% of the uptake in nuclei at 15 min, as well as 79.2% of the uptake at 2 h after ^(131)I-EB incubation. The DNA binding study demonstrated that evans blue(EB) has strong binding affinity with calf-thymus DNA(CT-DNA)(K_(sv)= 5.08×10~5 L/(mol/L)). Furthermore,the accumulation of ^(131)I-EB in necrotic muscle was efficiently blocked by an excess amount of unlabeled EB.In conclusion, ^(131)I-EB can not only detect necrosis by binding the DNA released from necrotic cells, but also image necrotic tissues generated from the disease clinically. | Qiaomei Jin Xin Shan Qi Luo Dongjian Zhang Yuanyu Zhao Nan Yao Fei Peng Dejian Huang Zhiqi Yin Wei Liu Jian Zhang | 2018 | Acta Pharmaceutica Sinica B2018，8，3： | 3 |
| 2 | Structural basis for gating mechanism of Pannexin 1 channel显示文摘Dear Editor,Pannexin 1(PANX1)plays extensive physiological roles across diverse fields of biology,including cell death,1,2 inflammation,3 cancer progression,4 and neurological disorders.5 As suggested by biochemical analysis,PANX1 forms an oligomeric channel for the facilitated diffusion of ions and large molecules across the plasma membrane upon activation.6 However,the underlying molecular gating mechanism of PANX1 and the structure of human PANX1 are still elusive.In the current study,we report the cryo-EM structures of full-length and carboxyl-terminal(CT)tail-cleaved human PANX1.Combined with single-channel electrophysiological study,we identified the key residues involved in the gating of PANX1. | Luqiu Mou Meng Ke Mengxiao Song Yuanyue Shan Qingjie Xiao Qingting Liu Jialu Li Ke Sun Lei Pu Li Guo Jia Geng Jianping Wu Dong Deng | 2020 | Cell Research2020，30，5： | 2 |
| 3 | Ionizable liposomal siRNA therapeutics enables potent and persistent treatment of Hepatitis B显示文摘Small interfering RNA(siRNA)constitutes a promising therapeutic modality supporting the potential functional cure of hepatitis B.A novel ionizable lipidoid nanoparticle(RBP131)and a state-of-the-art lyophilization technology were developed in this study,enabling to deliver siRNA targeting apolipoprotein B(APOB)into the hepatocytes with an ED_(50)of 0.05 mg/kg after intravenous injection.In addition,according to the requirements of Investigational New Drug(IND)application,a potent siRNA targeting hepatitis B virus(HBV)was selected and encapsulated with RBP131 to fabricate a therapeutic formulation termed RB-HBV008. | Yuanyu Huang Shuquan Zheng Zhaoxu Guo Xavier de Mollerat du Jeu Xing-Jie Liang Zhiwei Yang Hong-Yan Zhang Shan Gao Zicai Liang | 2022 | Signal Transduction and Targeted Therapy2022，7，3： | 1 |
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