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| 1 | Rapid and accurate detection of carbapenem-resistance gene by isothermal amplification in Acinetobacter baumannii显示文摘Background:Acinetobacter baumannii(A.baumannii)is one of the pivotal pathogens responsible for nosocomial infections,especially in patients with low immune response,and infection with carbapenem-resistant A.baumannii has been increasing in recent years.Rapid and accurate detection of carbapenem-resistance genes in A.baumannii could be of immense help to clinical staff.Methods:In this study,a 15-μL reaction system for recombinase polymerase amplification(RPA)was developed and tested.We collected 30 clinical isolates of A.baumannii from the Burn Institute of Southwest Hospital of Third Military Medical University(Army Medical University)for 6 months and tested antibiotic susceptibility using the VITEK 2 system.A.baumannii was detected based on the blaOXA-51 gene by PCR,qPCR and 15μL-RPA,respectively.Sensitivity and specificity were evaluated.In addition,PCR and 15μL-RPA data for detecting the carbapenem-resistance gene blaOXA-23 were comparatively assessed.Results:The detection limit of the blaOXA-51 gene by 15μL RPA was 2.86 CFU/ml,with sensitivity comparable to PCR and qPCR.No positive amplification signals were detected in non-Acinetobacter isolates,indicating high specificity.However,only 18 minutes were needed for the 15μL RPA assay.Furthermore,an antibiotic susceptibility test showed that up to 90%of A.baumannii strains were resistant to meropenem and imipenem;15μL RPA data for detecting blaOXA-23 showed that only 10%(n=3)of A.baumannii isolates did not show positive amplification signals,and the other 90%of(n=27)isolates were positive,corroborating PCR results.Conclusion:We demonstrated that the new 15μL RPA assay for detecting blaOXA-23 in A.baumannii is faster and simpler than qPCR and PCR.It is a promising alternative molecular diagnostic tool for rapid and effective detection of A.baumannii and drug-resistance genes in the field and point-ofcare testing. | Shuang Liu Guangtao Huang Yali Gong Xiaojun Jin Yudan Meng Yizhi Peng Junning Zhao Xiaolu Li Qin Li | 2020 | Burns & Trauma2020，8，1： | 2 |
| 2 | Neuronal injury and brain-derived neurotrophic factor expression in a rat model of amygdala kindling seizures Differences in brain regions and kindling courses显示文摘BACKGROUND:Studies have demonstrated that brain-derived neurotrophic factor(BDNF) has a dual effect on epilepsy.However,the relationship between epilepsy-induced brain injury and BDNF remains poorly understood.OBJECTIVE:According to ultrastructural and molecular parameters,to detect the degree of neuronal injury and BDNF expression changes at different brain regions and different kindling times to determine the effects of BDNF on epilepsy-induced brain injury.DESIGN,TIME AND SETTING:A randomized,controlled,animal experiment based on neuropathology and molecular biology was performed at the Department of Physiology and Department of Pathology,Basic Medical College of Jilin University in 2003.MATERIALS:UltraSensitive SP kit for immunohistochemistry(Fuzhou Maxim Biotechnology,China),BDNF antibody(concentrated type,Wuhan Boster Biological Technology,China),JEM-1000SX transmission electron microscopy(JEOL,Japan),and BH-2 light microscope(Olympus,Japan) were used in the present study.METHODS:Wistar rats were randomly assigned to control(n = 6),sham-surgery(n = 6),and model(n = 60) groups.The control group rats were not treated;an electrode was embedded into the amygdala in rats from the sham-surgery and model groups;an amygdala kindling epilepsy model was established in the model group.MAIN OUTCOME MEASURES:Pathological changes in the temporal lobe and hippocampus were observed by light and electron microscopy at 1,3,7,14,and 21 days following kindling,and BDNF expression in the various brain regions was determined by immunohistochemistry.RESULTS:In the model group,temporal lobe cortical and hippocampal neurons were swollen and the nuclei were laterally deviated.There were also some apoptotic neurons 3 days after kindling.The nucleoli disappeared and the nuclei appeared broken or lysed,as well as slight microglia hyperplasia,at 7 days.Electron microscopic observation displayed chromatin aggregation in the nuclei and slight mitochondrion swelling 3 days after kindling.Injury changes were aggravated at 7 days,characterized by broken cytoplasmic membrane and pyknosis.With the development of seizure,the number of BDNF-positive neurons in the hippocampus and temporal lobe increased and peaked at 7 days.Moreover,hippocampal and cortical temporal lobe injury continued.Following termination of electrical stimulation after 7 days of kindling,BDNF expression decreased,but continued to be expressed,up to 21 days of kindling.In addition,the number of temporal and hippocampal BDNF-positive neurons was greater than the control group.CONCLUSION:Brain injury and BDNF expression peaked at 7 days after kindling,and hippocampal changes were significant. | Weihong Lin Hongmei Meng Yudan Lv Zan Wang Na Li Li Cui Baimin Zhang | 2010 | Neural Regeneration Research2010，5，8： | 1 |
| 3 | Brain-derived neurotrophic factor expression is higher in brain tissue from patients with refractory epilepsy than in normal controls显示文摘The role of the brain-derived neurotrophic factor in epilepsy remains controversial.The present study utilized light and electron microscopy to investigate pathological and ultrastructural changes in brain tissue obtained from the seizure foci of 24 patients with temporal epilepsy.We found that epileptic tissue showed neuronal degeneration,glial cell proliferation,nuclear vacuolization,and neural cell tropism.Immunoelectron microscopy and immunohistochemistry showed that brain-derived neurotrophic factor was expressed at significantly higher levels in patients with refractory temporal epilepsy compared with normal controls,demonstrating that the pathological changes within seizure foci in patients with refractory epilepsy are associated with brain-derived neurotrophic factor expression alterations. | Yudan Lv Jiqing Qiu Zan Wang Li Cui Hongmei Meng Weihong Lin | 2011 | Neural Regeneration Research2011，6，29： | 0 |
| 4 | Roles and Molecular Mechanisms of Biomarkers in Hepatocellular Carcinoma with Microvascular Invasion: A Review显示文摘Hepatocellular carcinoma(HCC)being a leading cause of cancer-related death,has high associated mortality and recurrence rates.It has been of great necessity and urgency to find effective HCC diagnosis and treatment measures.Studies have shown that microvascular invasion(MVI)is an independent risk factor for poor prognosis after hepatectomy.The abnormal expression of biomacromolecules such as circ-RNAs,lncRNAs,STIP1,and PD-L1 in HCC patients is strongly correlated with MVI.Deregulation of several markers mentioned in this review affects the proliferation,invasion,metastasis,EMT,and anti-apoptotic processes of HCC cells through multiple complex mechanisms.Therefore,these biomarkers may have an important clinical role and serve as promising interventional targets for HCC.In this review,we provide a comprehensive overview on the functions and regulatory mechanisms of MVI-related biomarkers in HCC. | Xudong Zhao Yudan Wang Haoming Xia Shuqiang Liu Ziyue Huang Risheng He Liang Yu Nanfeng Meng Hang Wang Junqi You Jinglin Li Judy Wai Ping Yam Yi Xu Yunfu Cui | 2023 | Journal of Clinical and Translational Hepatology2023，11，5： | 0 |
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