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| 1 | In situ self-assembled organoid for osteochondral tissue regeneration with dual functional units显示文摘The regeneration of hierarchical osteochondral units is challenging due to difficulties in inducing spatial,directional and controllable differentiation of mesenchymal stem cells(MSCs)into cartilage and bone compartments.Emerging organoid technology offers new opportunities for osteochondral regeneration.In this study,we developed gelatin-based microcryogels customized using hyaluronic acid(HA)and hydroxyapatite(HYP),respectively for inducing cartilage and bone regeneration(denoted as CH-Microcryogels and OS-Microcryogels)through in vivo self-assembly into osteochondral organoids.The customized microcryogels showed good cytocompatibility and induced chondrogenic and osteogenic differentiation of MSCs,while also demonstrating the ability to self-assemble into osteochondral organoids with no delamination in the biphasic cartilage-bone structure.Analysis by mRNA-seq showed that CH-Microcryogels promoted chondrogenic differentiation and inhibited inflammation,while OS-Microcryogels facilitated osteogenic differentiation and suppressed the immune response,by regulating specific signaling pathways.Finally,the in vivo engraftment of pre-differentiated customized microcryogels into canine osteochondral defects resulted in the spontaneous assembly of an osteochondral unit,inducing simultaneous regeneration of both articular cartilage and subchondral bone.In conclusion,this novel approach for generating self-assembling osteochondral organoids utilizing tailor-made microcryogels presents a highly promising avenue for advancing the field of tissue engineering. | Zhen Yang Bin Wang Wei Liu Xiaoke Li Kaini Liang Zejun Fan Jiao Jiao Li Yudi Niu Zihao He Hui Li Du Wang Jianjing Lin Yanan Du Jianhao Lin Dan Xing | 2023 | Bioactive Materials2023，，9： | 2 |
| 2 | Ionic liquids on uncharged and charged surfaces: In situ microstructures and nanofriction显示文摘In situ changes in the nanofriction and microstructures of ionic liquids(ILs)on uncharged and charged surfaces have been investigated using colloid probe atomic force microscopy(AFM)and molecular dynamic(MD)simulations.Two representative ILs,[BMIM][BF_(4)](BB)and[BMIM][PF_(6)](BP),containing a common cation,were selected for this study.The torsional resonance frequency was captured simultaneously when the nanoscale friction force was measured at a specified normal load;and it was regarded as a measure of the contact stiffness,reflecting in situ changes in the IL microstructures.A higher nanoscale friction force was observed on uncharged mica and highly oriented pyrolytic graphite(HOPG)surfaces when the normal load increased;additionally,a higher torsional resonance frequency was detected,revealing a higher contact stiffness and a more ordered IL layer.The nanofriction of ILs increased at charged HOPG surfaces as the bias voltage varied from 0 to 8 V or from 0 to−8 V.The simultaneously recorded torsional resonance frequency in the ILs increased with the positive or negative bias voltage,implying a stiffer IL layer and possibly more ordered ILs under these conditions.MD simulation reveals that the[BMIM]+imidazolium ring lies parallel to the uncharged surfaces preferentially,resulting in a compact and ordered IL layer.This parallel“sleeping”structure is more pronounced with the surface charging of either sign,indicating more ordered ILs,thereby substantiating the AFM-detected stiffer IL layering on the charged surfaces.Our in situ observations of the changes in nanofriction and microstructures near the uncharged and charged surfaces may facilitate the development of IL-based applications,such as lubrication and electrochemical energy storage devices,including supercapacitors and batteries. | Rong AN Yudi WEI Xiuhua QIU Zhongyang DAI Muqiu WU Enrico GNECCO Faiz Ullah SHAH Wenling ZHANG | 2022 | Friction2022，10，11： | 0 |
| 3 | Myc inhibition tips the immune balance to promote antitumor immunity显示文摘Aberrant expression of Myc is one of the most common oncogenic events in human cancers.Scores of Myc inhibitors are currently under development for treating Myc-driven cancers.In addition to directly targeting tumor cells,Myc inhibition has been shown to modulate the tumor microenvironment to promote tumor regression.However,the effect of Myc inhibition on immune cells in the tumor microenvironment remains poorly understood.Here,we show that the adaptive immune system plays a vital role in the antitumor effect of pharmacologic inhibition of Myc.Combining genetic and pharmacologic approaches,we found that Myc inhibition enhanced CD8 T cell function by suppressing the homeostasis of regulatory T(Treg)cells and the differentiation of resting Treg(rTreg)cells to activated Treg(aTreg)cells in tumors.Importantly,we demonstrated that different Myc expression levels confer differential sensitivity of T cell subsets to pharmacologic inhibition of Myc.Although ablation of the Myc gene has been shown to suppress CD8 T cell function,Treg cells,which express much less Myc protein than CD8 T cells,are more sensitive to Myc inhibitors.The differential sensitivity of CD8 T and Treg cells to Myc inhibitors resulted in enhanced CD8 T cell function upon Myc inhibition.Our findings revealed that Myc inhibitors can induce an antitumor immune response during tumor progression. | Chao Yang Yun Liu Yudi Hu Liang Fang Zhe Huang Huanhuan Cui Jun Xie Yazhen Hong Wei Chen Nengming Xiao Qiyuan Li Wen-Hsien Liu Changchun Xiao | 2022 | Cellular & Molecular Immunology2022，19，9： | 0 |
| 4 | Genome-wide association analysis identified molecular markers and candidate genes for flower traits in Chinese orchid (Cymbidium sinense)显示文摘The orchid,the champagne of f lowers,brings luxury,elegance,and novelty to nature.Cymbidium sinense is a symbol of gigantic floral variability on account ofwavering shapes and sizes of f loral organs,althoughmarker-trait association(MTA)has not been studied for its f loral traits.We evaluated markers associated with 14 f loral traits of C.sinense through a genome-wide association study(GWAS)of 195 accessions.A total of 65318522 single-nucleotide polymorphisms(SNPs)and 3906176 insertion/deletion(InDel)events were identified through genotyping-by-sequencing.Among these,4694 potential SNPs and 477 InDelswere identified as MTAs at−log10 P>5.The genes related to these SNPs and InDels were largely associated with f loral regulators,hormonal pathways,cell division,and metabolism,playing essential roles in tailoring f loral morphology.Moreover,20 candidate SNPs/InDels linked to 11 genes were verified,8 of which were situated on exons,onewas located in the 5-UTR and twowere positioned in introns.Here,themultitepal trait-related gene RABBIT EARS(RBE)was found to be the most crucial gene.We analyzed the role of CsRBE in the regulation of flower-related genes via efficient transient overexpression in C.sinense protoplasts,and found that the floral homeotic genes CsAP3 and CsPI,as well as organ boundary regulators,including CsCUC and CsTCP genes,were regulated by CsRBE.Thus,we obtained key gene loci for important ornamental traits of orchids using genome-wide association analysis of populations with natural variation.The findings of this study can do a great deal to expedite orchid breeding programs for shape variability. | Fengxi Yang Yudi Guo Jie Li Chuqiao Lu Yonglu Wei Jie Gao Qi Xie Jianpeng Jin Genfa Zhu | 2023 | Horticulture Research2023，10，11： | 0 |
| 5 | Influence of open and closed windows on the vertical spread characteristics of fire显示文摘In order to systematically explain the spreading mechanism of vertical fire,Pyrosim software with fire dynamic modules is used to simulate the fire spreading characteristics in a five-storey residential apartment building.The vertical spread characteristics of fire,high-temperature smoke,and leap-frog behavior are analyzed by evaluating the distribution of pressure,temperature,and gas flow velocity in the studied numerical fire field.In addition,considering the possible cases of high-rise building fire in reality,the fire spreading characteristics of three different opening sequences of glass windows are given:(i)only the windows on the first floor are open;(ii)the windows are randomly opened by the high temperature of the glass;(iii)all glass windows from the first to the fifth floors are open.The simulation results show that an appropriate increase of turbulent flow in the low-level area can greatly reduce the fire temperature in the high-level area,which can provide a certain reference for the safety and design of building fire protection in the future. | Kai Wang Wei Zhao Yuan Yuan Yudie Jianyao Yanan Song Peifeng Hu Jingwei Huang | 2022 | Emergency Management Science and Technology2022，2，1： | 0 |
| 6 | Research on the Application of Intelligence Teaching Mode of Superstar Platform in the Teaching of Experimental Design and Data Processing显示文摘Experimental Design and Data Processing is an important core professional basic course for food science majors.This course is theoretical and practical,and there are many formulas,abstract contents and difficult to understand,and there are some problems in the teaching process,such as students1 poor interest in learning,insufficient mastery of what they have learned,and inability to combine theory with practice organically.Through analyzing the existing problems,this paper puts forward some reform measures for the teaching mode of experimental design and data processing by using the intelligent teaching of Superstar platform. | Wei Li Yudie Li Ran Wang Wenpin Lv Yonggang Qi | 2021 | Journal of Contemporary Educational Research2021，5，8： | 0 |
| 7 | Evaluation of the Momentum Closure Schemes in MPAS-Ocean显示文摘In order to compare and evaluate the performances of the Laplacian viscosity closure, the biharmonic viscosity closure, and the Leith closure momentum schemes in the MPAS-Ocean model, a variety of physical quantities, such as the relative reference potential energy(RPE) change, the RPE time change rate(RPETCR), the grid Reynolds number, the root mean square(RMS) of kinetic energy, and the spectra of kinetic energy and enstrophy, are calculated on the basis of results of a 3D baroclinic periodic channel. Results indicate that: 1) The RPETCR demonstrates a saturation phenomenon in baroclinic eddy tests. The critical grid Reynolds number corresponding to RPETCR saturation differs between the three closures: the largest value is in the biharmonic viscosity closure, followed by that in the Laplacian viscosity closure, and that in the Leith closure is the smallest. 2) All three closures can effectively suppress spurious dianeutral mixing by reducing the grid Reynolds number under sub-saturation conditions of the RPETCR, but they can also damage certain physical processes. Generally, the damage to the rotation process is greater than that to the advection process. 3) The dissipation in the biharmonic viscosity closure is strongly dependent on scales. Most dissipation concentrates on small scales, and the energy of small-scale eddies is often transferred to large-scale kinetic energy. The viscous dissipation in the Laplacian viscosity closure is the strongest on various scales, followed by that in the Leith closure. Note that part of the small-scale kinetic energy is also transferred to large-scale kinetic energy in the Leith closure. 4) The characteristic length scale L and the dimensionless parameter Г in the Leith closure are inherently coupled. The RPETCR is inversely proportional to the product of Г and L. When the product of Г and L is constant, both the simulated RPETCR and the inhibition of spurious dianeutral mixing are the same in all tests using the Leith closure. The dissipative scale in the Leith closure depends on the parameter L, and the dissipative intensity depends on the parameter Г. 5) Although optimal results may not be achieved by using the optimal parameters obtained from the 2D barotropic model in the 3D baroclinic simulation, the total energies are dissipative in all three closures. Dissipation is the strongest in the biharmonic viscosity closure, followed by that in the Leith closure, and that in the Laplacian viscosity closure is the weakest. Mesoscale eddies develop the fastest in the biharmonic viscosity closure after the baroclinic adjustment process finishes, and the kinetic energy reaches its maximum, which is attributed to the smallest dissipation of enstrophy in the biharmonic viscosity closure. Mesoscale eddies develop the slowest, and the kinetic energy peak value is the smallest in the Laplacian viscosity closure. Results in the Leith closure are between that in the biharmonic viscosity closure and the Laplacian viscosity closure. | ZHAO Shimei LIU Yudi LIU Wei | 2018 | Journal of Ocean University of China2018，17，2： | 0 |
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