| 1 | Application of fluorescence in situ hybridization in the detection of bladder transitional-cell carcinoma: A multi-center clinical study based on Chinese population显示文摘Objective:To evaluate the diagnostic value of fluorescence in situ hybridization(FISH)in bladder cancer.Methods:We enrolled healthy volunteers and patients who were clinically suspected to have bladder cancer and conducted FISH tests and cytology examinations from August 2007 to December 2008.Receiver operating characteristic(ROC)curve analysis was performed and the area under curve(AUC)values were calculated for both the FISH and urine cytology tests.Results:A cohort of 988 healthy volunteers was enrolled to establish a reference range for the normal population.A total of 4807 patients with hematuria were prospectively,randomly enrolled for the simultaneous analysis of urine cytology,FISH testing,and a final diagnosis as determined by the pathologic findings of a biopsy or a surgically-excised specimen.Overall,the sensitivity of FISH in detecting transitional-cell carcinoma was 82.7%,while that of cytology was 33.4%(p<0.001).The sensitivity values of FISH for non-muscle invasive and muscle invasive bladder transitional-cell carcinoma were 81.7%and 89.6%,respectively(p=0.004).The sensitivity values of FISH for low and high grade bladder cancer were 82.6%and 90.1%,respectively(p=0.002).Conclusion:FISH is significantly more sensitive than voided urine cytology for detecting bladder cancer in patients evaluated for gross hematuria at all cancer grades and stages.Higher sensitivity using FISH was obtained in high grade and muscle invasive tumors. | Liqun Zhou Kaiwei Yang Xuesong Li Yi Ding Dawei Mu Hanzhong Li Yong Yan Jinyi Li Dongwen Wang Wei Li Yulong Cong Jiangping Gao Kewei Ma Yajun Xiao Sheng Zhang Hongyi Jiang Weilie Hu Qiang Wei Xunbo Jin Zhichen Guan Qingyong Liu Danfeng Xu Xin Gao Yongguang Jiang Weimin Gan Guang Sun Qing Wang Yanhui Liu Jianquan Hou Liping Xie Xishuang Song Fengshuo Jin Jiafu Feng Ming Cai Zhaozhao Liang Jie Zhang Dingwei Ye Lin Qi Lulin Ma Jianzhong Shou Yuping Dai Jianyong Shao Ye Tian Shizhe Hong Tao Xu Chuize Kong Zefeng Kang Yuexin Liu Xun Qu Benkang Shi Shaobin Zheng Yi Lin Shujie Xia Dong Wei Jianbo Wu Weiling Fu Zhiping Wang Jianbo Liang | 2019 | Asian Journal of Urology2019，6，1： | 2 |
| 2 | RNA‑Sequencing Reveals Gene Expression and Pathway Signatures in Umbilical Cord Blood Affected by Birth Delivery Mode显示文摘Cesarean section(CS)confers increased risk of type I diabetes,asthma,inflammatory bowel disease,celiac disease,over-weight and obesity,etc.,in the offspring.However,the underlying mechanism remains unknown.To investigate the influence of CS on gene expression in cord blood,we have performed RNA-sequencing followed by single-gene analysis,gene set enrichment analysis,gene co-expression network analysis,and interacting genes/proteins analysis in eight full-term infants born by elective CS and eight matched vaginally delivered(VD)infants.Crucial genes identified above were further vali-dated in another 20 CS and 20 VD infants.We found for the first time that mRNA expression of genes involved in immune response(IL12A,INFG,IL1B,TNF,MIF,IL4,CA1,IFI27,HLA-DOB and EPHB1)and metabolism(DLK1,CYP2A6 and GATM)were significantly influenced by CS.Notably,serum TNF-αand IFN-γwere remarkably up-regulated in the CS infants(p=5.0×10^(–4)and 3.0×10^(–3),respectively)compared to the VD infants.It is biologically plausible that CS may exert adverse impacts on offspring health through influencing expression of genes in the above processes.These findings will help understand the potential underlying mechanisms of the adverse health impacts of CS and identify biomarkers for future health of offspring born with different delivery modes. | Yongjie Liu Kun Sun Yuexin Gan Han Liu Juehua Yu Wei Xu Lin Zhang Dan Chen | 2023 | Phenomics2023，3，3： | 0 |
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