|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | A Robust CRISPR/Cas9 System for Convenient, High-Efficiency Multiplex Genome Editing in Monocot and Dicot Plants显示文摘CRISPR/Cas9 染色体指向系统被用于许多种类。然而,为植物报导的 mostCRISPR/Cas9 系统能仅仅修改一个或一些指向地点。这里,我们报导一个柔韧的 CRISPR/Cas9 向量系统,利用植物 codon 优化了 Cas9 基因,为方便、高效率的复合染色体在 monocot 和 dicot 植物编辑。我们设计了基于 PCR 的过程很快产生多重 sgRNA 表达式盒子,它能在一个轮由金门结扎或吉布森汇编克隆被装配进二进制 CRISPR/Cas9 向量。与这个系统,我们与 85.4% 变化评估的一般水准在米饭编辑了 46 个目标地点,主要在 biallelic 和同型结合的地位。我们推想在米饭的大约 16% 同型结合的变化通过相应基于再结合的修理跟随的非相应的加入结束的机制被产生。我们也获得了一致 biallelic,在 Arabidopsis T 1 植物的异质接合、同型结合、妄想的变化。在米饭和 Arabidopsis 的指向的变化是可继承的。我们在 T 0 米饭和 T 1 由同时的指向的 Arabidopsis 植物多重(多达八) 一个基因家庭,在一条 biosynthetic 小径的多重基因,或在单个基因的多重地点的成员。这个系统为为基本研究和基因改进在植物学习多重基因和基因家庭的功能提供了一个万用的工具箱。 | Xingliang Ma Qunyu Zhang Qinlong Zhu Wei Liu Yan Chen Rong Qiu Bin Wang Zhongfang Yang Heying Li Yuru Lin Yongyao Xie Rongxin Shen Shuifu Chen Zhi Wang Yuanling Chen Jingxin Guo Letian Chen Xiucai Zhao Zhicheng Dong Yao-Guang Liu | 2015 | Molecular Plant2015，8，8： | 272 |
| 2 | Massive individual orbital angular momentum channels for multiplexing enabled by Dammann gratings显示文摘Data transmission rates in optical communication systems are approaching the limits of conventional multiplexing methods.Orbital angular momentum(OAM)in optical vortex beams offers a new degree of freedom and the potential to increase the capacity of free-space optical communication systems,with OAM beams acting as information carriers for OAM division multiplexing(OAM-DM).We demonstrate independent collinear OAM channel generation,transmission and simultaneous detection using Dammann optical vortex gratings(DOVGs).We achieve 80/160 Tbit s^(-1) capacity with uniform power distributions along all channels,with 1600 individually modulated quadrature phase-shift keying(QPSK)/16-QAM data channels multiplexed by 10 OAM states,80 wavelengths and two polarizations.DOVG-enabled OAM multiplexing technology removes the bottleneck of massive OAM state parallel detection and offers an opportunity to raise optical communication systems capacity to Pbit s^(-1) level. | Ting Lei Meng Zhang Yuru Li Ping Jia Gordon Ning Liu Xiaogeng Xu Zhaohui Li Changjun Min Jiao Lin Changyuan Yu Hanben Niu Xiaocong Yuan | 2015 | Light(Science & Applications)2015，4，1： | 47 |
| 3 | A Text Sentiment Classification Modeling Method Based on Coordinated CNN-LSTM-Attention Model显示文摘The major challenge that text sentiment classification modeling faces is how to capture the intrinsic semantic, emotional dependence information and the key part of the emotional expression of text. To solve this problem, we proposed a Coordinated CNNLSTM-Attention(CCLA) model. We learned the vector representations of sentence with CCLA unit. Semantic and emotional information of sentences and their relations are adaptively encoded to vector representations of document.We used softmax regression classifier to identify the sentiment tendencies in the text. Compared with other methods, the CCLA model can well capture the local and long distance semantic and emotional information.Experimental results demonstrated the effectiveness of CCLA model. It shows superior performances over several state-of-the-art baseline methods. | ZHANG Yangsen ZHENG Jia JIANG Yuru HUANG Gaijuan CHEN Ruoyu | 2019 | Chinese Journal of Electronics2019，28，1： | 32 |
| 4 | Study of Sentiment Classification for Chinese Microblog Based on Recurrent Neural Network显示文摘The sentiment classification of Chinese Microblog is a meaningful topic.Many studies has been done based on the methods of rule and word-bag,and to understand the structure information of a sentence will be the next target.We proposed a sentiment classification method based on Recurrent neural network(RNN).We adopted the technology of distributed word representation to construct a vector for each word in a sentence;then train sentence vectors with fixed dimension for different length sentences with RNN,so that the sentence vectors contain both word semantic features and word sequence features;at last use softmax regression classifier in the output layer to predict each sentence's sentiment orientation.Experiment results revealed that our method can understand the structure information of negative sentence and double negative sentence and achieve better accuracy.The way of calculating sentence vector can help to learn the deep structure of sentence and will be valuable for different research area. | ZHANG Yangsen JIANG Yuru TONG Yixuan | 2016 | Chinese Journal of Electronics2016，25，4： | 29 |
| 5 | Development of a Hand Exoskeleton System for Index Finger Rehabilitation显示文摘In order to overcome the drawbacks of traditional rehabilitation method,the robot-aided rehabilitation has been widely investigated for the recent years.And the hand rehabilitation robot,as one of the hot research fields,remains many challenging issues to be investigated.This paper presents a new hand exoskeleton system with some novel characteristics.Firstly,both active and passive rehabilitative motions are realized.Secondly,the device is elaborately designed and brings advantages in many aspects.For example,joint motion is accomplished by a parallelogram mechanism and high level motion control is therefore made very simple without the need of complicated kinematics.The adjustable joint limit design ensures that the actual joint angles don't exceed the joint range of motion(ROM) and thus the patient safety is guaranteed.This design can fit to the different patients with different joint ROM as well as to the dynamically changing ROM for individual patient.The device can also accommodate to some extent variety of hand sizes.Thirdly,the proposed control strategy simultaneously realizes the position control and force control with the motor driver which only works in force control mode.Meanwhile,the system resistance compensation is preliminary realized and the resisting force is effectively reduced.Some experiments were conducted to verify the proposed system.Experimentally collected data show that the achieved ROM is close to that of a healthy hand and the range of phalange length(ROPL) covers the size of a typical hand,satisfying the size need of regular hand rehabilitation.In order to evaluate the performance when it works as a haptic device in active mode,the equivalent moment of inertia(MOI) of the device was calculated.The results prove that the device has low inertia which is critical in order to obtain good backdrivability.The experiments also show that in the active mode the virtual interactive force is successfully feedback to the finger and the resistance is reduced by one-third;for the passive control mode,the desired trajectory is realized satisfactorily. | LI Jiting WANG Shuang WANG Ju ZHENG Ruoyin ZHANG Yuru CHEN Zhongyuan | 2012 | Chinese Journal of Mechanical Engineering2012，25，2： | 12 |
| 6 | The chromosome-level reference genome assembly for Panax notoginseng and insights into ginsenoside biosynthesis显示文摘Panax notoginseng,a perennial herb of the genus Panax in the family Araliaceae,has played an important role in clinical treatment in China for thousands of years because of its extensive pharmacological effects.Here,we report a high-quality reference genome of P.notoginseng,with a genome size up to 2.66 Gb and a contig N50 of 1.12 Mb,produced with third-generation PacBio sequencing technology.This is the first chromosome-level genome assembly for the genus Panax.Through genome evolution analysis,we explored phylogenetic and whole-genome duplication events and examined their impact on saponin biosynthesis.We performed a detailed transcriptional analysis of P.notoginseng and explored genelevel mechanisms that regulate the formation of characteristic tubercles.Next,we studied the biosynthesis and regulation of saponins at temporal and spatial levels.We combined multi-omics data to identify genes that encode key enzymes in the P.notoginseng terpenoid biosynthetic pathway.Finally,we identified five glycosyltransferase genes whose products catalyzed the formation of different ginsenosides in P.notoginseng.The genetic information obtained in this study provides a resource for further exploration of the growth characteristics,cultivation,breeding,and saponin biosynthesis of P.notoginseng. | Zhouqian Jiang Lichan Tu Weifei Yang Yifeng Zhang Tianyuan Hu Baowei Ma Yun Lu Xiuming Cui Jie Gao Xiaoyi Wu Yuru Tong Jiawei Zhou Yadi Song Yuan Liu Nan Liu Luqi Huang Wei Gao | 2021 | Plant Communications2021，2，1： | 11 |
| 7 | Ammonium sulfide-assisted hydrothermal activation of palygorskite for enhanced adsorption of methyl violet显示文摘Herein,palygorskite(PAL)was activated via a simple hydrothermal process in the presence of ammonium sulfide,and the effects of activation on the microstructure,physico-chemical feature and adsorption behaviors of PAL were intensively investigated.The hydrothermal process evidently improved the dispersion of PAL crystal bundles,increased surface negative charges and built more active –Si–O-groups served as the new'adsorption sites'.The adsorption property of the activated PAL for Methyl Violet(MV)was systematically investigated by optimizing the adsorption variables,including p H,ionic strength,contact time and initial MV concentration.The activated PAL exhibited a superior adsorption capability to the raw PAL for the removal of MV(from 156.05 to 218.11 mg/g).The kinetics for MV adsorption followed pseudo second-order kinetic models,while the isotherm and thermodynamics results showed that the adsorption pattern well followed the Langmuir model.The structure analysis of PAL before and after adsorption demonstrated that electrostatic interaction and chemical association of –X–O-are the prominent driving forces for the adsorption process. | Guangyan Tian Wenbo Wang Yuru Kang Aiqin Wang | 2016 | Journal of Environmental Sciences2016，28，3： | 6 |
| 8 | ECONOMIC GROWTH AND CYCLES IN MODERN CHINA显示文摘If the theory of cyclical fluctuation can be applied to modern China, the economic development ofChina in this period can be seen as consisting of several stages. At each stage as well as in the wholeperiod, chosen index system is likely to help measure the growth rate. By vertical and horizontal com-parison, this paper will try to settle the disputes on China’s modern economic development. | Liu Foding Wang Yuru Yu Jianwei 1937- Professor of Institute of Economics of NANKAI UNIVERSITY. Supervisor to Ph. D. candidates Ph. D. of Economics. Associate Professor of Institute of Economics of NANKAI UNIVERSITY Master of economics. Lecturer of Department of Economics atSICHUAN UNIVERSITY | 1994 | 南开经济研究1994，，S3： | 6 |
| 9 | Change of Soil Organic Carbon after Cropland Afforestation in ′Beijing-Tianjin Sandstorm Source Control′ Program Area in China显示文摘Land use change is one of the major factors that affect soil organic carbon(SOC) variation and global carbon balance. However, the effects of land use change on SOC are always variable. In this study, using a series of paired-field experiments, we estimated the effects of revegetation types and environmental conditions on SOC stock and vertical distribution after replacement of cropland with poplar(Populus tomentosa) and korshinsk peashrub(Caragana korshinskii) in three climate regions(Chifeng City, Fengning City and Datong City of the ′Beijing-Tianjin Sandstorm Source Control′(BTSSC) program area. The results show that SOC sequestration rate ranges from 0.15 Mg/(ha·yr) to 3.76 Mg/(ha·yr) in the soil layer of 0–100 cm in early stage after cropland afforestation in the BTSSC program area. The SOC accumulation rates are the highest in Fengning for both the two vegetation types. Compared to C. korshinskii, P. tomentosa has greater effects on SOC accumulation in the three climate regions, but significantly greater effect only appears in Datong. The SOC density increases by 20%–111% and 15%–59% for P. tomentosa and 9%–63% and 0–73% for C. korshinskii in the 0–20 cm and 20–100 cm soil layers, respectively. Our results indicate that cropland afforestation not only affects SOC stock in the topsoil, but also has some effects on subsoil carbon. However, the effect of cropland afforestation on SOC accumulation varied with climate regions and revegetation types. Considering the large area of revegetation and relatively high SOC accumulation rate, SOC sequestration in the BTSSC program should contribute significantly to decrease the CO2 concentration in the atmosphere. | ZENG Xinhua ZHANG Wanjun LIU Xiuping CAO Jiansheng SHEN Huitao ZHAO Xin ZHANG Nan-nan BAI Yuru Yi Mei | 2014 | Chinese Geographical Science2014，24，4： | 6 |
| 10 | Buckling and post-buckling behavior of titanium alloy stiffened panels under shear load显示文摘Titanium alloy has been increasingly applied in aviation industry due to its superior performance. However, the titanium alloy structures are less studied. This work investigates the structural behavior of Ti6Al4V titanium alloy stiffened panels under in-plane shear load by experiments and numerical analysis. After the shear tests, the buckling instability, the post-buckling process and the failure mechanism of the specimen were obtained. The Finite Element(FE) models were established with the subsequent validation verification. A parametric analysis was implemented to study the influence of stringer thickness and stringer height on the behavior of the stiffened panels. The results show that after the initial local buckling on the skin, the buckling mode jumps several times with the increase of load. The stringers twist when the load reaches a certain level, and finally the structure damages due to the plastic deformation and the global buckling. The shear clip has little effect on the buckling and failure loads. Compared to the relatively large effect on the buckling load, the influence of the stringer thickness and stringer height on the failure load is neglectable.According to the parametric analysis, the stringer thickness influences the final buckling mode and failure mode, while the stringer height affects the buckling mode transformation. | Yuru SU Zhidong GUAN Xin WANG Zengshan LI Jun GUO Yongjie HUANG | 2019 | Chinese Journal of Aeronautics2019，32，3： | 5 |
| 11 | Molecular cloning and functional identification of a cDNA encoding 4-hydroxy-3-methylbut-2-enyl diphosphate reductase from Tripterygium wilfordii显示文摘The 4-hydroxy-3-methylbut-2-enyl diphosphate reductase(HDR) is the last step key enzyme of the methylerythritol phosphate(MEP) pathway,synthesizing isopentenyl diphosphate and its allyl isomer dimethylallyl diphosphate,which is important for regulation of isoprenoid biosynthesis.Here the full-length cDNA of HDR,designated TwHDR(GenBank Accession No.KJ933412.1),was isolated from Tripterygium wilfordii for the first time.TwHDR has an open reading frame(ORF) of 1386 bp encoding461 amino acids.TwHDR exhibits high homology with HDRs of other plants,with an N-terminal conserved domain and three conserved cysteine residues.TwHDR cDNA was cloned into an expression vector and transformed into an Escherichia coli hdr mutant.Since loss-of-function E.coli hdr mutant is lethal,the result showed that transformation of TwHDR cDNA rescued the E.coli hdr mutant.This complementation assay suggests that the TwHDR cDNA encodes a functional HDR enzyme.The expression of TwHDR was induced by methyl-jasmonate(MJ) in T.wilfordii suspension cells.The expression of TwHDR reached the highest level after 1 h of MJ treatment.These results indicate that we have identified a functional TwHDR enzyme,which may play a pivotal role in the biosynthesis of diterpenoid triptolide in T.wilfordii. | Qiqing Cheng Yuru Tong Zihao Wang Ping Su Wei Gao Luqi Huang | 2017 | Acta Pharmaceutica Sinica B2017，7，2： | 5 |
| 12 | Research on Android Malware Detection and Interception Based on Behavior Monitoring显示文摘Focusing on the sensitive behaviors of malware, such as privacy stealing and money costing, this paper proposes a new method to monitor software behaviors and detect malicious applications on Android platform. According to the theory and implementation of Android Binder interprocess communication mechanism, a prototype system that integrates behavior monitoring and intercepting, malware detection, and identification is built in this work. There are 50 different kinds of samples used in the experiment of malware detection, including 40 normal samples and 10 malicious samples. The theoretical analysis and experimental result demonstrate that this system is effective in malware detection and interception, with a true positive rate equal to 100% and a false positive rate less than 3%. | PENG Guojun SHAO Yuru WANG Taige ZHAN Xian ZHANG Huanguo | 2012 | Wuhan University Journal of Natural Sciences2012，17，5： | 5 |
| 13 | Management of China's capture fisheries:Review and prospect显示文摘Management of capture fisheries has been a perpetual problem facing all fisheries countries worldwide.This study first analyzes the features of China's capture fisheries,and points out that its management tends to be more complex and difficult than any other country in the world.It goes on to describe the history of China's fisheries management and the development process of China's fisheries policies,recognizing the role of fisheries policies in guiding and promoting industrial production during the whole development process.With Fisheries Law of the People's Republic of China as the basic framework,China is thought to have initially formed a fisheries management system with a complete structure and powerful measures for constant improvement and reinforcement.Major systems and measures of China's fisheries management are summarized,with their corresponding effects being evaluated to a certain extent.China's continuous introduction of advanced fisheries management philosophies over the past decades,especially since the reform and opening up,plays an active role in securing the prosperity of the following five industries:aquaculture,capture fisheries,processing and logistics,enhancement,and recreational fisheries;more effort has been put into fisheries ecological environment restoration;and lawbased governance capacity in fisheries has been significantly improved.However,due to the characteristics of China's fisheries,as well as the complex and uncertain nature of fisheries itself,certain prominent problems still remain in its capture fisheries.With reference to relevant fisheries development planning,the study concludes with a prospect that China's capture fisheries management will be steered towards the total catch control of fisheries resources and the intensification of resource conservation and ecological restoration. | Shuolin Huang Yuru He | 2019 | Aquaculture and Fisheries2019，4，5： | 4 |
| 14 | Comparing containment measures among nations by epidemiological effects of COVID-19显示文摘The coronavirus disease 2019(COVID-19)has evolved to a global pandemic since its inception in December 2019.Countries have responded to the epidemics with different levels of responses and containment measures.Given the unprecedented pressure on nations’healthcare systems and the deaths so far,there is an urgent need to evaluate the effects of the containm ent measures,which would be useful for countries to plan for their responses to counter the first or possibly the second wave of the epidemic.There have been studies for the effects of COVID-19 control measures taken in China on disease transmission and public health interventions for W uhan’s outbreaks[1-4],and W uhan travel ban on the spread of COVID-19 both inside China and internationally[5,6]. | Jia Gu Han Yan Yaxuan Huang Yuru Zhu Haoxuan Sun Yumou Qiu Songxi Chen | 2020 | National Science Review2020，7，12： | 4 |
| 15 | Molecular cloning and functional identification of sterol C24-methyltransferase gene from Tripterygium wilfordii显示文摘Sterol C24-methyltransferase(SMT) plays multiple important roles in plant growth and development. SMT1, which belongs to the family of transferases and transforms cycloartenol into 24-methylene cycloartenol, is involved in the biosynthesis of 24-methyl sterols. Here, we report the cloning and characterization of a cDNA encoding a sterol C24-methyltransferase from Tripterygium wilfordii(Tw SMT1). Tw SMT1(Gen Bank access number KU885950) is a 1530 bp cDNA with a 1041 bp open reading frame predicted to encode a 346-amino acid, 38.62 k Da protein. The polypeptide encoded by the SMT1 cDNA was expressed and purified as a recombinant protein from Escherichia coli(E. coli) and showed SMT activity. The expression of Tw SMT1 was highly up-regulated in T. wilfordii cell suspension cultures treated with methyl jasmonate(Me JA). Tissue expression pattern analysis showed higher expression in the phellem layer compared to the other four organs(leaf, stem, xylem and phloem), which is about ten times that of the lowest expression in leaf. The results are meaningful for the study of sterolbiosynthesis of T. wilfordii and will further lay the foundations for the research in regulating both the content of other main compounds and growth and development of T. wilfordii. | Hongyu Guan Yujun Zhao Ping Su Yuru Tong Yujia Liu Tianyuan Hu Yifeng Zhang Xianan Zhang Jia Li Xiaoyi Wu Luqi Huang Wei Gao | 2017 | Acta Pharmaceutica Sinica B2017，7，5： | 4 |
| 16 | Efects of solution chemistry on arsenic(V) removal by low-cost adsorbents显示文摘Natural and anthropogenic arsenic(As)contamination of water sources pose serious health concerns,especially for small communities in rural areas.This study assessed the applicability of three industrial byproducts(coal fly ash,lignite,and green waste compost)as the low-cost adsorbents for As(V)removal under various field-relevant conditions(dissolved oxygen,As(V)/Fe ratio,solution pH,and presence of competing species).The physico-chemical properties of the adsorbents were characterized by XRD,XRF,FT-IR,and NMR analysis.Batch experiments demonstrated that coal fly ash could provide efective As(V)removal(82.1%–95%)because it contained high content of amorphous iron/aluminium hydroxides for As(V)adsorption and dissolvable calcium minerals for calcium arsenate precipitation.However,the addition of lignite and green waste compost was found unfavourable since they hindered the As(V)removal by 10%–42%possibly due to dissolution of organic matter and ternary arsenate-iron-organic matter complexes.On the other hand,higher concentrations of dissolved iron(comparing As(V)/Fe ratios of 1:1 and 1:10)and dissolved oxygen(comparing 0.2 and 6 mg/L)only marginally enhanced the As(V)removal at pH 6 and 8.Thus,addition of dissolved iron,water aeration,or pH adjustment became unnecessary because coal fly ash was able to provide efective As(V)removal under the natural range of geochemical conditions.Moreover,the presence of low levels of background competing(0.8 or 8 mg/L of humic acid,phosphate,and silicate)imposed little influence on As(V)removal,possibly because the high adsorption capacity of coal fly ash was far from exhaustion.These results suggested that coal fly ash was a potentially promising adsorbent that warranted further investigation. | Yuru Wang Daniel C.W.Tsang | 2013 | Journal of Environmental Sciences2013，25，11： | 4 |
| 17 | Design of a Lightweight Force-Feedback Glove with a Large Workspace显示文摘A wearable force-feedback glove is a promising way to enhance the immersive sensation when a user interacts with virtual objects in virtual reality scenarios.Design challenges for such a glove include allowing a large fingertip workspace,providing a desired force sensation when simulating both free-and constrained-space interactions,and ensuring a lightweight structure.In this paper,we present a forcefeedback glove using a pneumatically actuated mechanism mounted on the dorsal side of the user’s hand.By means of a triple kinematic paired link with a curved sliding slot,a hybrid cam-linkage mechanism is proposed to transmit the resistance from the pneumatic piston rod to the fingertip.In order to obtain a large normal component of the feedback force on the user’s fingertip,the profile of the sliding slot was synthesized through an analysis of the force equilibrium on the triple kinematic paired link.A prototype five-fingered glove with a mass of 245 g was developed,and a wearable force-measurement system was constructed to permit the quantitative evaluation of the interaction performance in both free and constrained space.The experimental results confirm that the glove can achieve an average resistance of less than 0.1 N in free-space simulation and a maximum fingertip force of 4 N in constrained-space simulation.The experiment further confirms that this glove permits the finger to move freely to simulate typical grasping gestures. | Yukai Zheng Dangxiao Wang Ziqi Wang Yu Zhang Yuru Zhang Weiliang Xu | 2018 | Engineering2018，4，6： | 2 |
| 18 | Phosphatase activity and culture conditions of the yeast Candida mycoderma sp. and analysis of organic phosphorus hydrolysis ability显示文摘Orthophosphate is an essential but limiting macronutrient for plant growth. About 67%cropland in China lacks sufficient phosphorus, especially that with red soil. Extensive soil phosphorus reserves exist in the form of organic phosphorus, which is unavailable for root uptake unless hydrolyzed by secretory acid phosphatases. Thus, many microorganisms with the ability to produce phosphatase have been exploited. In this work, the activity of an extracellular acid phosphatase and yeast biomass from Candida mycoderma was measured under different culture conditions, such as p H, temperature, and carbon source. A maximal phosphatase activity of 8.47 × 105± 0.11 × 105U/g was achieved by C. Mycoderma in 36 hr under the optimal conditions. The extracellular acid phosphatase has high activity over a wide p H tolerance range from 2.5 to 5.0(optimum p H 3.5). The effects of different phosphorus compounds on the acid phosphatase production were also studied. The presence of phytin, lecithin or calcium phosphate reduced the phosphatase activity and biomass yield significantly. In addition, the p H of the culture medium was reduced significantly by lecithin. The efficiency of the strain in releasing orthophosphate from organic phosphorus was studied in red soil(used in planting trees) and rice soil(originating as red soil). The available phosphorus content was increased by 230% after inoculating20 days in rice soil and decreased by 50% after inoculating 10 days in red soil. This work indicates that the yeast strain C. mycoderma has potential application for enhancing phosphorus utilization in plants that grow in rice soil. | Mang Yan Liufang Yu Liang Zhang Yuexia Guo Kewei Dai Yuru Chen | 2014 | Journal of Environmental Sciences2014，26，11： | 2 |
| 19 | Chromosome doubling mediates superior drought tolerance in Lycium ruthenicum via abscisic acid signaling显示文摘Plants are continuously affected by unfavorable external stimuli,which influences their productivity and growth.Differences in gene composition and expression patterns lead homologous polyploid plants to exhibit different physiological phenomena,among which enhanced environmental adaptability is a powerful phenotype conferred by polyploidization.The mechanisms underlying the differences in stress tolerance between diploids and autotetraploids at the molecular level remain unclear.In this research,a full-length transcription profile obtained via the singlemolecule real-time(SMRT)sequencing of high-quality single RNA molecules for use as background was combined with next-generation transcriptome and proteome technologies to probe the variation in the molecular mechanisms of autotetraploids.Tetraploids exhibited an increase in ABA content of 78.4%under natural conditions and a superior stress-resistance phenotype under severe drought stress compared with diploids.The substantial differences in the transcriptome profiles observed between diploids and autotetraploids under normal growth conditions were mainly related to ABA biosynthesis and signal transduction pathways,and 9-cis-epoxycarotenoid dioxygenase 1(NCED1)and NCED2,which encode key synthetic enzymes,were significantly upregulated.The increased expression of the ABREbinding factor 5-like(ABF5-like)gene was a pivotal factor in promoting the activation of the ABA signaling pathway and downstream target genes.In addition,ABA strongly induced the expression of osmotic proteins to increase the stress tolerance of the plants at the translational level.We consider the intrinsic mechanisms by which ABA affects drought resistance in tetraploids and diploids to understand the physiological and molecular mechanisms that enhance abiotic stress tolerance in polyploid plants. | Shupei Rao Yuru Tian Xinli Xia Yue Li Jinhuan Chen | 2020 | Horticulture Research2020，7，1： | 2 |
| 20 | The coloring mechanism of a novel golden variety in Populus deltoides based on the RGB color mode显示文摘Compared to flower color and red leaf mutants,the mechanism of yellow leaf formation in woody plants is more complicated and less understood.Poplars are common and widely distributed perennial trees.Here,we obtained a golden leaf mutant poplar variety(JHY)and compared it with its original green leaf poplar(Populus sp.Linn.‘2025’)in terms of phenotype,pigment content,the transcriptomes and metabolomes.Through transcriptome sequencing,we found that the chlorophyll degradation genes,and the genes in anthocyanin metabolism in JHY were up-regulated compared to L2025 and the carotenoid synthesis genes were down-regulated.Further based on HPLC-ESI-MS/MS technology,16 differentially expressed anthocyanins were identified,14 reddish anthocyanins of which were significantly up-regulated.According to these results,we proposed a coloring mechanism of JHY based on the RGB color mode.The yellow color of carotenoids and the red color of anthocyanins overlay each other,combined with a reduction in chlorophyll,turning the leaves golden.This study systematic analyzes the causes of golden leaf formation through the omics integration approach,which will provide reference for the breeding of golden leaf trees. | Yuru Tian Shupei Rao Qianqian Li Meng Xu Aike Wang Hechen Zhang Jinhuan Chen | 2021 | Forestry Research2021，1，1： | 2 |
您所在的机构暂未开通此部分的功能，请联系管理员