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| 1 | A Robust CRISPR/Cas9 System for Convenient, High-Efficiency Multiplex Genome Editing in Monocot and Dicot Plants显示文摘CRISPR/Cas9 染色体指向系统被用于许多种类。然而,为植物报导的 mostCRISPR/Cas9 系统能仅仅修改一个或一些指向地点。这里,我们报导一个柔韧的 CRISPR/Cas9 向量系统,利用植物 codon 优化了 Cas9 基因,为方便、高效率的复合染色体在 monocot 和 dicot 植物编辑。我们设计了基于 PCR 的过程很快产生多重 sgRNA 表达式盒子,它能在一个轮由金门结扎或吉布森汇编克隆被装配进二进制 CRISPR/Cas9 向量。与这个系统,我们与 85.4% 变化评估的一般水准在米饭编辑了 46 个目标地点,主要在 biallelic 和同型结合的地位。我们推想在米饭的大约 16% 同型结合的变化通过相应基于再结合的修理跟随的非相应的加入结束的机制被产生。我们也获得了一致 biallelic,在 Arabidopsis T 1 植物的异质接合、同型结合、妄想的变化。在米饭和 Arabidopsis 的指向的变化是可继承的。我们在 T 0 米饭和 T 1 由同时的指向的 Arabidopsis 植物多重(多达八) 一个基因家庭,在一条 biosynthetic 小径的多重基因,或在单个基因的多重地点的成员。这个系统为为基本研究和基因改进在植物学习多重基因和基因家庭的功能提供了一个万用的工具箱。 | Xingliang Ma Qunyu Zhang Qinlong Zhu Wei Liu Yan Chen Rong Qiu Bin Wang Zhongfang Yang Heying Li Yuru Lin Yongyao Xie Rongxin Shen Shuifu Chen Zhi Wang Yuanling Chen Jingxin Guo Letian Chen Xiucai Zhao Zhicheng Dong Yao-Guang Liu | 2015 | Molecular Plant2015，8，8： | 272 |
| 2 | Massive individual orbital angular momentum channels for multiplexing enabled by Dammann gratings显示文摘Data transmission rates in optical communication systems are approaching the limits of conventional multiplexing methods.Orbital angular momentum(OAM)in optical vortex beams offers a new degree of freedom and the potential to increase the capacity of free-space optical communication systems,with OAM beams acting as information carriers for OAM division multiplexing(OAM-DM).We demonstrate independent collinear OAM channel generation,transmission and simultaneous detection using Dammann optical vortex gratings(DOVGs).We achieve 80/160 Tbit s^(-1) capacity with uniform power distributions along all channels,with 1600 individually modulated quadrature phase-shift keying(QPSK)/16-QAM data channels multiplexed by 10 OAM states,80 wavelengths and two polarizations.DOVG-enabled OAM multiplexing technology removes the bottleneck of massive OAM state parallel detection and offers an opportunity to raise optical communication systems capacity to Pbit s^(-1) level. | Ting Lei Meng Zhang Yuru Li Ping Jia Gordon Ning Liu Xiaogeng Xu Zhaohui Li Changjun Min Jiao Lin Changyuan Yu Hanben Niu Xiaocong Yuan | 2015 | Light(Science & Applications)2015，4，1： | 47 |
| 3 | On Strengthening Chinese College Students’Audience Awareness to Improve Their Persuasive Writing Ability显示文摘Persuasive or argumentative writing,which focuses on the reader and seeks to change the reader’s mind,or to bring the reader’s point of view to the writer’s,is the most prominent and practical academic writing and is also the most complex and most challenging.To achieve the persuasive purpose,audience is a key element to consider.But unfortunately,many Chinese college students have no sense of audience at all.Besides,although some researchers have mentioned in their books or papers the important role the audience plays in persuasive writing,so far few writing books or papers have made a relatively comprehensive and systematic study and exploration of how to strengthen Chinese college students’audience awareness.So,this paper,which is intended to elaborate on it,first gives a definition to audience awareness,then analyzes the influence audience awareness exerts on persuasive writing,and finally proposes a few feasible and effective methods to strengthen Chinese college students’audience awareness. | SHEN Yuru LIN Li | 2023 | Sino-US English Teaching2023，20，2： | 0 |
| 4 | Adding power of artificial intelligence to situational awareness of large interconnections dominated by inverter‐based resources显示文摘Large‐scale power systems exhibit more complex dynamics due to the increasing inte-gration of inverter‐based resources(IBRs).Therefore,there is an urgent need to enhance the situational awareness capability for better monitoring and control of power grids dominated by IBRs.As a pioneering Wide‐Area Measurement System,FNET/GridEye has developed and implemented various advanced applications based on the collected synchrophasor measurements to enhance the situational awareness capability of large‐scale power grids.This study provides an overview of the latest progress of FNET/GridEye.The sensors,communication,and data servers are upgraded to handle ultra‐high density synchrophasor and point‐on‐wave data to monitor system dynamics with more details.More importantly,several artificial intelligence(AI)‐based advanced appli-cations are introduced,including AI‐based inertia estimation,AI‐based disturbance size and location estimation,AI‐based system stability assessment,and AI‐based data authentication. | Lin Zhu Yinfeng Zhao Yi Cui Shutang You Wenpeng Yu Shengyuan Liu He Yin Chang Chen Yuru Wu Wei Qiu Mirka Mandich Hongyu Li Adedasola Ademola Chengwen Zhang Chujie Zeng Xinlan Jia Weikang Wang Haoyu Yuan Huaiguang Jiang Jin Tan Yilu Liu | 2021 | High Voltage2021，6，6： | 0 |
| 5 | LDL receptor-related protein 1 (LRP1),a novel target for opening the blood-labyrinth barrier(BLB)显示文摘Inner ear disorders are a cluster of diseases that cause hearing loss in more than 1.5 billion people worldwide.However,the presence of the blood-labyrinth barrier(BLB)on the surface of the inner ear capillaries greatly hinders the effectiveness of systemic drugs for prevention and intervention due to the low permeability,which restricts the entry of most drug compounds from the bloodstream into the inner ear tissue.Here,we report the finding of a novel receptor,low-density lipoprotein receptor-related protein 1(LRP1),that is expressed on the BLB,as a potential target for shuttling therapeutics across this barrier.As a proof-ofconcept,we developed an LRP1-binding peptide,IETP2,and covalently conjugated a series of model small-molecule compounds to it,including potential drugs and imaging agents.All compounds were successfully delivered into the inner ear and inner ear lymph,indicating that targeting the receptor LRP1 is a promising strategy to enhance the permeability of the BLB.The discovery of the receptor LRP1 will illuminate developing strategies for crossing the BLB and for improving systemic drug delivery for inner ear disorders. | Xi Shi Zihao Wang Wei Ren Long Chen Cong Xu Menghua Li Shiyong Fan Yuru Xu Mengbing Chen Fanjun Zheng Wenyuan Zhang Xinbo Zhou Yue Zhang Shiwei Qiu Liyuan Wu Peng Zhou Xinze Lv Tianyu Cui Yuehua Qiao Hui Zhao Weiwei Guo Wei Chen Song Li Wu Zhong Jian Lin Shiming Yang | 2022 | Signal Transduction and Targeted Therapy2022，7，7： | 0 |
| 6 | Impact time control guidance against maneuvering targets based on a nonlinear virtual relative model显示文摘Aiming at the problem of high-precision interception of air-maneuvering targets with impact time constraints,this paper proposes a novel guidance law based on a nonlinear virtual relative model in which the origin is attached to the target.In this way,the original maneuvering target is transformed into a stationary one.A polynomial function of the guidance command in the range domain with two unknown coefficients is introduced into the virtual model,one of the coefficients is determined to achieve the impact time constraint,and the other is determined to satisfy a newly defined virtual look angle constraint.For meeting the terminal constraints simultaneously,the guidance command can finally be obtained.The resulting solution is represented as a combination of proportional navigation guidance-like term which is aimed to meet the zero miss distance constraint,a bias term for impact time control by adjusting the length of the homing trajectory,and an additional term for target maneuvers.Numerous simulations demonstrate that the proposed law achieves an acceptable impact time error for various initial conditions against different types of maneuvering targets and shows more effective performance in comparison with those of other existing guidance laws. | Yuru BIN Hui WANG Defu LIN Yaning WANG Xin SUN | 2023 | Chinese Journal of Aeronautics2023，36，7： | 0 |
| 7 | Developmental and epileptic encephalopathy 44 due to compound heterozygous variants in the UBA5 gene:a case report显示文摘Background Developmental and epileptic encephalopathy(DEE)is a group of rare inherited disorders characterized by intellectual disability,delayed development,epileptic seizures,and other related symptoms.DEE44 is caused by mutations in the UBA5 gene,which encodes a ubiquitin-like protein involved in protein degradation and cell signaling.However,there is limited information on the genotype–phenotype correlation of DEE44,and its clinical features remain to be fully characterized.Case presentation We report a 12-month-old infant who presented with epileptic spastic seizures beginning at 4 months of age,accompanied by overall developmental delay,short stature,microcephaly,inability to hold his head upright,chasing vision,and high muscle tone in the extremities.Genetic findings showed compound heterozygous mutations of the UBA5 gene:NM_024818 c.562C>T(p.R188X)from the mother and NM_024818 c.214C>T(p.R72C)from the father.Conclusions This case report expands the clinical spectrum of DEE44 and highlights the importance of considering DEE44 in the differential diagnosis of developmental delay and epilepsy,even in the absence of classical symptoms suggestive of the condition.We hope that this case report will advance the understanding of DEE44 and improve the expertise of clinicians and early diagnose of this disease. | Suli Zhang Shuangzhu Lin Wanqi Wang Yuru Gan Cui Wang Bangtao Li Qiming Pang | 2023 | Acta Epileptologica2023，6，4： | 0 |
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