|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | A draft sequence of the rice (Oryza sativa ssp. indica) genome显示文摘The sequence of the rice genome holds fundamental information for its biology, including physiology, genetics, development, and evolution, as well as information on many beneficial phenotypes of economic significance. Using a 'whole genome shotgun' approach, we have pro-duced a draft rice genome sequence of Oryza sativa ssp. in-dica, the major crop rice subspecies in China and many other regions of Asia. The draft genome sequence is constructed from over 4.3 million successful sequencing traces with an accumulative total length of 2214.9 Mb. The initial assembly of the non-redundant sequences reached 409.76 Mb in length, based on 3.30 million successful sequencing traces with a total length of 1797.4 Mb from an indica variant cultivar 93-11, giving an estimated coverage of 95.29% of the rice genome with an average base accuracy of higher than 99%. The coverage of the draft sequence, the randomness of the sequence distribution, and the consistency of BIG-ASSEM-BLER, a custom-designed software package | YU Jun, HU Songnian, WANG Jun,LI Songgang WONG Ka-Shu Gane, LIU Bin,DENG Yajun, DAI Li, ZHOU Yan,ZHANG Xiuqing, CAO Mengliang, LIU Jing,SUN Jiandong , TANG Jiabin, CHEN Yanjiong,HUANG Xiaobing, LIN Wei, YE Chen, TONG Wei,CONG Lijuan, GENG Jianing, HAN Yujun, LI Lin,LI Wei, HU Guangqiang, HUANG Xiangang,LI Wenjie, LI Jian, LIU Zhanwei, LI Long,LIU Jianping, Ql Qiuhui, LIU Jinsong, LI Li,WANG Xuegang, LU Hong, WU Tingling,ZHU Miao, Nl Peixiang, HAN Hua, DONG Wei,REN Xiaoyu, FENG Xiaoli, GUI Peng,LI Xianran, WANG Hao, XU Xin, ZHAI Wenxue,XU Zhao, ZHANG Jinsong, HE Sijie,ZHANG Jianguo, XU Jichen, ZHANG Kunlin,ZHENG Xianwu, DONG Jianhai, ZENG Wanyong,TAO Lin, CHEN Xuewei, HE Jun, LIU Daofeng,TIAN Wei, TIAN Chaoguang, XIA Hongai,LI Gang, GAO Hui, LI Ping, CHEN Wei ,WANG Xudong, ZHANG Yong, HU Jianfei,WANG Jing, LIU Song, YANG Jian,ZHANG Guangyu, XIONG Yuqing, LI Zhijie,MAO Long, ZHOU Chengshu, ZHU Zhen,CHEN Runsheng, HAO Bailin,ZHENG Weimou, CHEN Shouyi, QUO Wei,LI Guojie, LIU Siqi, HUANG Guyang,TAO Ming, WANG Jian, ZHU Lihuang,YUAN Longping& YANG HuanmingBeijing Genomics Institute/Center of Genomics & Bioinformatics, Chinese Academy of Sciences, Beijing 101300, China Hangzhou Genomics Institute/Institute of Bioinformatics of Zhejiang University/Key Laboratory of Bioinformatics of Zhejiang Province, Hangzhou 310007, China Institute of Genetics, Chinese Academy of Sciences, Beijing 100101, China National Hybrid Rice R & D Center, Changsha 410125, China Laboratory of Bioinformatics, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China College of Life Sciences, Peking University, Beijing 100871, China Institute of Theoretical Physics, Chinese Academy of Sciences, Beijing 1Q0080, China Digital China Ltd., Beijing 100080, China Institute of Computing Technology, Chinese Academy of Sciences, Beijing 100080, China Medical College, Xi’an Jiaotong University, Xi’an 710061, ChinaThese authors contributed equally to this work.Corresponding author.Corresponden | 2001 | Chinese Science Bulletin2001，46，23： | 6 |
| 2 | Adapting rice anther culture to gene transformation and RNA interference显示文摘Anther culture offers a rapid method of generating homozygous lines for breeding pro- gram and genetic analysis.To produce homozygous transgenic lines of rice(Oryza sativa L.)in one step,we developed an efficient protocol of anther-callus-based transformation mediated by Agro- bacterium after optimizing several factors influencing efficient transformation,including callus induc- tion and Agrobacterium density for co-cultivation.Using this protocol,we obtained 145 independent green transformants from five cultivars of japonica rice by transformation with a binary vector pCXK1301 bearing the rice gene,Xa21 for resistance to bacterial blight,of which 140 were further confirmed by PCR and Southern hybridization analysis,including haploids(32.1%),diploids(62.1%) and mixoploids(7.5%).Fifteen diploids were found to be doubled haploids,which accounted for 10.7%of the total positive lines.Finally,by including 28 from colchicine induced or spontaneous dip- loidization of haploids later after transformation,a total of 43 doubled haploids(30.7%)of Xa21 transgenic lines were obtained.We also generated two RNAi transgenic haploids of the rice Os- MADS2 gene,a putative redundant gene of OsMADS4 based on their sequence similarity,to inves- tigate its possible roles in rice flower development by this method.Flowers from the two OsMADS2 RNAi transgenic haploids displayed obvious homeotic alternations,in which lodicules were trans- formed into palea/lemma-like tissues,whereas identities of other floral organs were maintained.The phenotypic alternations were proved to result from specific transcriptional suppression of OsMADS2 gene by the introduced RNAi transgene.The results confirmed that OsMADS2 is involved in lodicule development of rice flower and functionally redundant with OsMADS4 gene.Our results demonstrated that rice anther culture could be adapted to gene transformation and RNAi analysis in rice. | CHEN Caiyan 1,2* ,XIAO Han 1,2* ,ZHANG Wenli 1,2 ,WANG Aiju 1,2 ,XIA Zhihui 1 ,LI Xiaobing 1 , ZHAI Wenxue 1 ,CHENG Zhukuan 1 &ZHU Lihuang 1 1.State Key Laboratory of Plant Genomics and National Center for Plant Gene Research,Institute of Genetics and Develop- mental Biology,Chinese Academy of Sciences,Beijing 100101,China 2.Graduate School of the Chinese Academy of Sciences,Beijing 100039,China | 2006 | Science China(Life Sciences)2006，49，5： | 5 |
| 3 | Detecting Integer Overflow Vulnerabilities in Binary Executables Based on Target Filtering and Dynamic Taint Tracing显示文摘The number of identified integer overflow vulnerabilities has been increasing rapidly in recent years.In this paper, a smart software vulnerability detection technology is presented, which is used for the identification of integer overflow vulnerabilities in binary executables. The proposed algorithm is combined with Target filtering and dynamic taint tracing(TFDTT). Dynamic taint tracing is used to reduce the mutation space and target filtering function is used to filter test cases during the process of test case generation. Theory analysis indicates that the efficiency of TFDTT is higher than Non TF-DTT and random Fuzzing technology. And the experiment results indicate that the detection technology based upon TFDTT can identify the possible integer vulnerabilities in binary program, meanwhile, it is more efficiency than other two technologies. | CUI Baojiang LIANG Xiaobing ZHAO Bing ZHAI Feng WANG Jianxin | 2014 | Chinese Journal of Electronics2014，23，2： | 2 |
| 4 | Forecast and control of anode shape in electrochemical machining using neural network显示文摘 | PANG Duibing XU Wenji ZHAI Xiaobing | 2004 | Lecture Notes in Computer Science2004，3174，： | 1 |
| 5 | An improved asso- ciation rules mining method 显示文摘 | Liu Xiaobing Zhai Kun Witold Pedrycz | 2012 | Expert Systems with Applica- tions2012，39，1： | 1 |
| 6 | Fine-tuning of pore-space-partitioned metal-organic frameworks for efficient C_(2)H_(2)/C_(2)H_(4) and C_(2)H_(2)/CO_(2) separation显示文摘Acetylene (C_(2)H_(2)) and ethylene (C_(2)H_(4)) both are important chemical raw materials and energy fuel gasses.But the effective removement of trace C_(2)H_(2)from C_(2)H_(4)and the purification of C_(2)H_(2)from carbon dioxide(CO_(2)) are particularly challenging in the petrochemical industry.As a class of porous physical adsorbent,metal-organic frameworks (MOFs) have exhibited great success in separation and purification of light hydrocarbon gas.Herein,we rationally designed four novel MOFs by the strategy of pore space partition(PSP) via introducing triangular tri(pyridin-4-yl)-amine (TPA) into the 1D hexagonal channels of acs-type parent skeleton.By modulating the functional groups of linear dicarboxylate linkers for the parent skeleton,a series of isoreticular PSP-MOFs (SNNU-278-281) were successfully obtained.The synergistic effects of suitable pore size and Lewis basic functional groups make these MOFs ideal C_(2)H_(2)adsorbents.The gas adsorption experimental results show that all MOFs have excellent C_(2)H_(2)uptakes.Specially,SNNU-278demonstrates a high C_(2)H_(2)uptake of 149.7 cm3/g at 273 K and 1 atm.Meanwhile,SNNU-278-281 MOFs also show extremely great C_(2)H_(2)separation from CO_(2)and C_(2)H_(4).The optimized SNNU-281 with highdensity hydroxy groups exhibits extraordinary C_(2)H_(2)/CO_(2)and C_(2)H_(2)/C_(2)H_(4)dynamic breakthrough interval times up to 31 min/g and 17 min/g under 298 K and 1 bar. | Xiaobing Mu Yingying Xue Mancheng Hu Peng Zhang Ying Wang Haipeng Li Shuni Li Quanguo Zhai | 2023 | Chinese Chemical Letters2023，34，2： | 0 |
| 7 | A RKKY Model Fitting of Nuclear Magnetic Resonance Spectra of ^(63)Cu in Fe/Cu Multilayers显示文摘In this letter,a theoretical fit of nuclear magnetic resonance spectra of ^(63)Cu in Cu layers of Fe/Cu multilayers is given based on RKKY theory.The values of exchange field and additional Knight shift are fitted,which agree approximately with the experimental data. | ZHAI Ya ZHU Xiaobing HU Chen ZHU Jiangang LU Mu ZHAI Hongru | 1995 | Chinese Physics Letters1995，12，4： | 0 |
您所在的机构暂未开通此部分的功能，请联系管理员