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| 1 | Thermo-responsive gelatin-functionalized PCL film surfaces for improvement of cell adhesion and intelligent recovery of gene-transfected cells显示文摘Efficient local gene transfection on a tissue scaffold is dependent on good cell-adhesion characteristics. In this work, the thermoresponsive gelatin-functionalized polycaprolactone(PCL) films were proposed for improvement of cell adhesion and intelligent recovery of gene-transfected cells. Functional copolymer brushes(PCL-g-P(NIPAAm-co-MAAS)) were first prepared via surface-initiated ATRP of N-isopropylacrylamide(NIPAAm) and methacrylic acid sodium salt(MAAS) from the initiatorfuncationalized PCL surfaces. The pendant carboxyl end-groups of the PCL-g-P(NIPAAm-co-MAAS) surface were subsequently coupled with gelatin via carbodiimide chemistry to produce the thermo-responsive gelatin-functionalized PCL surface. The thermo-responsive gelatin-functionalized PCL film surface can improve cell adhesion and proliferation above the LCST of P(NIPAAm) without destroying cell detachment properties at lower temperatures. The dense transfected cells can be recovered simply by lowering culture temperature. The thermo-responsive gelatin-functionalized PCL films are potentially useful as intelligent adhesion modifiers for directing cellular functions within tissue scaffolds. | YUAN Wei ZHAO NaNa YU BingRan JIANG Hui MA Jie XU FuJian | 2014 | Science China Chemistry2014，57，4： | 2 |
| 2 | Osteoblast integration of dental implant materials after challenge by sub-gingival pathogens: a co-culture study in vitro显示文摘Sub-gingival anaerobic pathogens can colonize an implant surface to compromise osseointegration of dental implants once the soft tissue seal around the neck of an implant is broken. In vitro evaluations of implant materials are usually done in monoculture studies involving either tissue integration or bacterial colonization. Co-culture models, in which tissue cells and bacteria battle simultaneously for estate on an implant surface, have been demonstrated to provide a better in vitro mimic of the clinical situation. Here we aim to compare the surface coverage by U2 OS osteoblasts cells prior to and after challenge by two anaerobic sub-gingival pathogens in a co-culture model on differently modified titanium(Ti), titanium-zirconium(Ti Zr) alloys and zirconia surfaces. Monoculture studies with either U2 OS osteoblasts or bacteria were also carried out and indicated significant differences in biofilm formation between the implant materials, but interactions with U2 OS osteoblasts were favourable on all materials. Adhering U2 OS osteoblasts cells, however,were significantly more displaced from differently modified Ti surfaces by challenging sub-gingival pathogens than from Ti Zr alloys and zirconia variants. Combined with previous work employing a co-culture model consisting of human gingival fibroblasts and supra-gingival oral bacteria, results point to a different material selection to stimulate the formation of a soft tissue seal as compared to preservation of osseointegration under the unsterile conditions of the oral cavity. | Bingran Zhao Henny C van der Mei Minie Rustema-Abbing Henk J Busscher Yijin Ren | 2015 | International Journal of Oral Science2015，7，4： | 1 |
| 3 | Great progress in molecular breeding of hybrid rice in CNHRRDC显示文摘 | Zhao Bingran Yuan Longping | 2005 | Aguricul Science & Technology2005，6，3： | 1 |
| 4 | Lyase Activities of Heterologous CpcS and CpcT for Phycocyanin Holo-β-subunit from Arthrospira platensis in Escherichia coli显示文摘Arthrospira platensis is an economically important cyanobacterium; and it has been used widely in food and pharmaceutical industries. The phycocyanin(PC) from A. platensis is extremely valuable in medicine and molecular biology due to its antioxidation and anti-tumoring activity and applicability as fluorescence protein tag. In present study, two recombinant plasmids, one contained the phycocyanobilin(PCB)-producing genes(hox1 and pcyA) while the other contained the phycobiliprotein gene(cpcB) and the lyase gene(either cpcS/U or cpcT), were constructed and synchronically transferred into E. coli in order to test the the activities of relevant lyases for catalysing PCB addition to CpcB during synthesizing fluorescent PC holo-β-subunit(β-PC) of A. platensis. As was evidenced by the fluorescence emitted at a peak specific for PC, CpcB was successfully synthesized in E. coli, to which co-expressed PCBs attached though at a relatively low efficiency. The results showed that the attachment of PCBs to CpcB were carried out mainly by co-expressed CpcS/U but CpcB also showed some autocatalytic activity. Currently, no CpcT activity was detected in this E. coli expression system. Further studies will be conducted to improve the efficiency of fluorescent PC synthesis in E. coli. | YI Junjie XU Di ZANG Xiaonan YUAN Dingyang ZHAO Bingran TANG Li TAN Yanning ZHANG Xuecheng | 2014 | Journal of Ocean University of China2014，13，3： | 1 |
| 5 | Great progress in molecular breeding of hybrid rice in CNHRRDC显示文摘 | Zhao Bingran Yuan Longping | 2005 | Agricultural Science &Technologe2005，6，3： | 1 |
| 6 | Heterosis in root microbiota inhibits growth of soil-borne fungal pathogens in hybrid rice显示文摘In nature,plants are colonized by various microbes that play essential roles in their growth and health.Heterosis is a natural genetic phenomenon whereby first-generation hybrids exhibit superior phenotypic performance relative to their parents.It remains unclear whether this concept can be extended to the“hybridization”of microbiota from two parents in their descendants and what benefits the hybrid microbiota might convey.Here,we investigated the structure and function of the root microbiota from three hybrid rice varieties and their parents through amplicon sequencing analysis of bacterial 16S ribosomal DNA(rDNA)and fungal internal transcribed spacer(ITS)regions.We show that the bacterial and fungal root microbiota of the varieties are distinct from those of their parental lines and exhibit potential heterosis features in diversity and composition.Moreover,the root bacterial microbiota of hybrid variety LYP9 protects rice against soil-borne fungal pathogens.Systematic analysis of the protective capabilities of individual strains from a 30-member bacterial synthetic community derived from LYP9 roots indicated that community members have additive protective roles.Global transcription profiling analyses suggested that LYP9 root bacterial microbiota activate rice reactive oxygen species production and cell wall biogenesis,contributing to heterosis for protection.In addition,we demonstrate that the protection conferred by the LYP9 root microbiota is transferable to neighboring plants,potentially explaining the observed hybrid-mediated superior effects of mixed planting.Our findings suggest that some hybrids exhibit heterosis in their microbiota composition that promotes plant health,highlighting the potential for microbiota heterosis in breeding hybrid crops. | Mengting Zhang Yinyue Wang Yuanyi Hu Huacai Wang Yawen Liu Bingran Zhao Jie Zhang Rongxiang Fang Yongsheng Yan | 2023 | Journal of Integrative Plant Biology2023，65，4： | 0 |
| 7 | Practical Realization of Optimal Auxiliary Frequency Control Strategy of Wind Turbine Generator显示文摘Adding the auxiliary frequency control function to the wind turbine generator(WTG)is a solution to the frequency security problem of the power system caused by the replacement of the synchronous generator(SG)by the WTG.The auxiliary frequency control using rotor kinetic energy is an economical scheme because the WTG still runs at the maximum power point during normal operation.In this paper,the functional optimization model of the auxiliary frequency control strategy of WTG is established.The optimal auxiliary frequency control strategy is obtained by solving the model numerically.As for the practical realization of the control strategy,the coordination of the auxiliary frequency control with the maximum power point tracking(MPPT)control is studied.The practical auxiliary frequency control strategy is modified to adapt to different power disturbances in the system,and the parameter setting method is also proposed.The sensitivity of system frequency to control parameters is studied.Finally,the simulation results verify the effectiveness and practicability of the proposed control strategy. | Ming Sun Yong Min Xuejun Xiong Lei Chen Le Zhao Yuyao Feng Bingran Wang | 2022 | Journal of Modern Power Systems and Clean Energy2022，10，3： | 0 |
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