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| 1 | Foliar δ^(13)C and δ^(15)N values of C_3 plants in the Ethiopia Rift Valley and their environmental controls显示文摘The foliar C and N stable isotopic compositions(δ 13C and δ 15N) and the relationships between these compositions and environmental factors of C3 plants in the Ethiopia Rift Valley were investigated. There were three distribution patterns for foliar δ 13C with mean values of ?26.7‰±0.4‰,?29.7‰± 0.6‰,and ?26.9‰± 1.2‰ in cold-moist,temperate-moist,and arid-hot environments,respectively. The δ 15N values ranged from ?1.4‰± 1.7‰ to 14.3‰± 0.1‰,with higher values under arid-hot conditions and the lowest values in plants growing at higher altitudes under cold-moist conditions. A strong negative relationship between mean annual precipitation and δ 15N explained more than half of the observed variation in the δ 15N values(r2 = 0.54,P < 0.001);a modest positive relationship was also found between δ 15N and tem-perature(r2 = 0.32,P < 0.01). A weakly positive relationship existed between δ 13C and temperature,and changes in δ 13C values with precipitation and altitude followed quadratic curves. This suggests a shift in the effects of water and heat conditions caused by altitude on carbon isotopic discrimination. | LIU XiaoHong ZHAO LiangJu GASAW Menassie GAO DengYi QIN DaHe REN JiaWen | 2007 | Chinese Science Bulletin2007，52，9： | 12 |
| 2 | Climatic significance of the stable carbon isotope composition of tree-ring cellulose:Comparison of Chinese hemlock (Tsuga chinensis Pritz) and alpine pine (Pinus densata Mast) in a temperate-moist region of China显示文摘In the reconstruction of past climate using stable carbon isotope composition (δ13C) in tree ring,the responses of the stable carbon composition (δ13C) of multiple tree species to environmental factors must be known detailedly. This study presented two δ13C series in annual tree rings for Chinese hem-lock (Tsuga chinensis Pritz) and alpine pine (Pinus densata Mast),and investigated the relationships between climatic parameters and stable carbon discrimination (△13C) series,and evaluated the poten-tial of climatic reconstruction using △13C in both species,in a temperate-moist region of Chuanxi Pla-teau,China. The raw δ13C series of the two species was inconsistent,which may be a result of different responses caused by tree's inherent physiological differences. After removing the low-frequency ef-fects of CO2 concentration,the high-frequency (year-to-year) inter-series correlation of △13C was strong,indicating that △13C of the two tree species were controlled by common environmental conditions. The △13C series of the species were most significantly correlated with temperature and moisture stress,but in different periods and intensity between the species. During the physiological year,the impacts of temperature and moisture stress on △13C occur earlier for Chinese hemlock (previous December to February for moisture stress and February to April for temperature,respectively) than for alpine pine (March to May for moisture stress and April to July for temperature,respectively). In addition,in temperate-moist regions,the control on △13C of single climatic parameter was not strongly dominant and the op-timal multiple regressions functions just explained the 38.5% variance of the total. Therefore,there is limited potential for using δ13C alone to identify clear,reliable climatic signals from two species. | LIU XiaoHong SHAO XueMei WANG LiLi ZHAO LiangJu WU Pu CHEN Tuo QIN DaHe REN JiaWen | 2007 | Science China Earth Sciences2007，50，7： | 10 |
| 3 | Harnessing the native type I-B CRISPR-Cas for genome editing in a polyploid archaeon显示文摘Research on CRISPR-Cas(clustered regularly interspaced short palindromic repeats-CRISPR associated protein) systems has led to the revolutionary CRISPR/Cas9 genome editing technique. However, for most archaea and half of bacteria, exploitation of their native CRISPR-Cas machineries may be more straightforward and convenient. In this study, we harnessed the native type I-B CRISPR-Cas system for precise genome editing in the polyploid haloarchaeon Haloarcula hispanica. After testing different designs, the editing tool was optimized to be a single plasmid that carries both the self-targeting miniCRISPR and a 600-800 bp donor. Significantly, chromosomal modifications, such as gene deletion, gene tagging or single nucleotide substitution, were precisely introduced into the vast majority of the transformants. Moreover, we showed that simultaneous editing of two genomic loci could also be readily achieved by one step. In summary, our data demonstrate that the haloarchaeal CRISPR-Cas system can be harnessed for genome editing in this polyploid archaeon, and highlight the convenience and efficiency of the native CRISPR-based genome editing strategy. | Feiyue Cheng Luyao Gong Dahe Zhao Haibo Yang Jian Zhou Ming Li Hua Xiang | 2017 | Journal of Genetics and Genomics2017，44，11： | 5 |
| 4 | From highly polluted inland city of China to'Lanzhou Blue':The air-pollution characteristics显示文摘Air quality was improved considerably and the so-called'Lanzhou Blue'appeared frequently in Lanzhou due to implementation of some strict emission-control measures in recent years.To better understand whether the concentration of each air pollutant had decreased significantly and then give some suggestions as to urban air-quality improvement in the near future,the variations of the Air Quality Index(AQI)and six criterion air pollutants(PM_(2.5),PM_(10),CO,SO_2,NO_2,and O_3)at five state-controlled monitoring sites of Lanzhou were studied from 2013 to 2016.The AQI,PM_(2.5),PM_(10),and SO_2 gradually decreased from 2013 to 2016,while CO and NO_2 concentrations had slightly increasing trends,especially in urban areas,due to the large number of motor vehicles,which had an annual growth rate of 30.87%.The variations of the air pollutants in the no-domestic-heating season were more significant than those in the domestic-heating season.The increase of ozone concentration for the domestic-heating season at a background station was the most significant among the five monitoring sites.The vehicle-exhaust and ozone pollution was increasingly severe with the rapid increase in the number of motor vehicles.The particulate-matter pollution became slight in the formerly highly polluted Lanzhou City.Some synergetic measures in urban and rural areas of Lanzhou should be taken by the local government in the near future to control fine particulate-matter(PM_(2.5))and ozone pollution. | SuPing Zhao Ye Yu DaHe Qin | 2018 | Research in Cold and Arid Regions2018，10，1： | 2 |
| 5 | Haloarchaeal-Type β-Ketothiolases Involved in Poly(3-Hydroxybutyrate-co-3-Hydroxyvalerate) Synthesis in Haloferax mediterranei显示文摘 | Jing Hou Bo Feng Jing Han Hailong Liu Dahe Zhao Jian Zhou Hua Xiang | 2013 | Applied and Environmental Microbiology2013，，17： | 1 |
| 6 | Improving polyhydroxyalkanoate production by knocking out the genes involved in exopolysaccharide biosynthesis in Haloferax mediterranei显示文摘 | Dahe Zhao Lei Cai Jinhua Wu Ming Li Hailong Liu Jing Han Jian Zhou Hua Xiang | 2013 | Applied Microbiology and Biotechnology2013，，7： | 1 |
| 7 | Cellular and organellar membrane-associated proteins in haloarchaea: Perspectives on the physiological significance and biotechnological applications显示文摘Halophilic archaea (haloarchaea) inhabit hypersaline environments,tolerating extreme salinity,low oxygen and nutrient availability,and in some cases,high pH (soda lakes) and irradiation (saltern ponds).Membrane-associated proteins of haloarchaea,such as surface layer (S-layer) proteins,transporters,retinal proteins,and internal organellar membrane proteins including intracellular gas vesicle proteins and those associated with polyhydroxyalkanoate (PHA) granules,contribute greatly to their environmental adaptations.This review focuses on these haloarchaeal cellular and organellar membrane-associated proteins,and provides insight into their physiological significance and biotechnological potential. | CAI Lei ZHAO DaHe HOU Jing WU JinHua CAI ShuangFeng DASSARMA Priya XIANG Hua | 2012 | Science China(Life Sciences)2012，55，5： | 0 |
| 8 | Carbon Metabolism and Bioplastic Production by Halophilic Archaea显示文摘1 Introduction Haloarchaea represents a distinct group of Archaea that typically inhabits hypersaline environments,such as salt lakes and sea salterns.They are easy to culture and many haloarchaea are genetically tractable,hence they are excellent model systems for research of archaeal genetics, | XIANG Hua HAN Jing HOU Jing ZHAO Dahe CAI Shuangfeng CAI Lei | 2014 | Acta Geologica Sinica(English Edition)2014，88，S1： | 0 |
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