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| 1 | Autologous CD34^+ and CD133^+ stem cells transplantation in patients with end stage liver disease显示文摘AIM:To assess the utility of an autologous CD34 + and CD133 + stem cells infusion as a possible therapeutic modality in patients with end-stage liver diseases.METHODS:One hundred and forty patients with endstage liver diseases were randomized into two groups.Group 1,comprising 90 patients,received granulocyte colony stimulating factor for five days followed by autologous CD34 + and CD133 + stem cell infusion in the portal vein.Group 2,comprising 50 patients,received regular liver treatment only and served as a control group.RESULTS:Near normalization of liver enzymes and improvement in synthetic function were observed in 54.5% of the group 1 patients;13.6% of the patients showed stable states in the infused group.None of the patients in the control group showed improvement.No adverse effects were noted.CONCLUSION:Our data showed that a CD34 + and CD133 + stem cells infusion can be used as supportive treatment for end-stage liver disease with satisfactory tolerability. | Hosny Salama Abdel-Rahman N Zekri Abeer A Bahnassy Eman Medhat Hanan A Halim Ola S Ahmed Ghada Mohamed Sheren A Al Alim Ghada M Sherif | 2010 | World Journal of Gastroenterology2010，16，42： | 16 |
| 2 | Treatment responses in Asians and Caucasians with chronic hepatitis C infection显示文摘AIM:To conduct a multicentre retrospective review of virological response rates in Asians infected with genotype 1 chronic hepatitis C(CHC) treated with combination interferon and ribavirin and then to compare their responses to that among Caucasians.METHODS:Asian patients infected with genotype 1 CHC treated at 4 Australian centres between 2001 to 2005 were identified through hospital databases.Baseline demographic characteristics,biochemical,virological and histological data and details of treatment were collected.Sustained virological responses(SVR) in this cohort were then compared to that in Caucasian subjects,matched by genotype,age,gender and the stage of hepatic fibrosis.RESULTS:A total of 108 Asians with genotype 1 CHC were identified.The end of treatment response(ETR) for the cohort was 79% while the SVR was 67%.Due to the relatively advanced age of the Asian cohort,only sixty-four subjects could be matched with Caucasians.The ETR among matched Asians and Caucasians was 81% and 56% respectively(P=0.003),while the SVR rates were 73% and 36%(P <0.001) respectively.This difference remained significant after adjusting for other predictive variables. | Kenneth K Yan Marianne Guirgis Amany Zekry Thuy Dinh Anouk Dev Jacob George Alice Lee | 2008 | World Journal of Gastroenterology2008，14，21： | 12 |
| 3 | Mismatch repair genes (hMLH1, hPMS1, hPMS2, GTBP/hMSH6,HMSH2) in the pathogenesis of hepatocellular carcinoma显示文摘AIM: DNA misrnatch repair (MMR) is an impotent mechanism for maintaining fidelity of genomic DNA. Abnormalities in one or more MMR genes are implicated in the development of many cancers. We investigated the role of expression of MMR genes (hMLH1, hPMS1, hPMS2, GTBP/hMSH6, hMSH2) in hepatocellular carcinogenesis. METHODS: We evaluated the expression level of MMR genes in 33 hepatocellular carcinoma (HCC) cases using the multiplex reverse transcription (RT) PCR assays, as well as in 16 cases of normal adjacent hepatic tissues. Β-actin gene was used as an internal control and calibrator for quantification of gene expression. RESULTS: Out of the 33 studied cases, 25 were HCV positiveand 30 (90.9%) showed reduced expression in one or more of the studied MMR genes. Reduced expression was found in hMSH2 (71.9%), hMLH1 (53.3%), GTBP (51.1%), hPMS2 (33.3%) and hPMS1 (6%). A significant correlation was found between reduced expression of hPMS2(P= 0.0069) and GTBP(P= 0.0034), hPMS2and non-cirrhosis (P= 0.0197),hMLH1 and high grade. On the other hand, 57.1%, 50%, 20%, 18.8%, and 6% of the normal tissues distant to tumors showed reduced expression of hMSH2, hMLH1, GTBP, hPMS2, and hPMS1 respectively. Multivariate analysis revealed a significant correlation between the expression level of hMSH2(P= 0.008), hMLH1 (P= 0.001) and GTBP (P = 0.032)and HCC, between hPMS2, GTBP and HCVassociated HCC (P<0.001, 0.002).CONCLUSION: Reduced expression of MMR genes seemsto play an important role in HCV-associated HCC. hPMS2 islikely involved at an early stage of hepatocarcinogenesis since it was detected in normal adjacent tissues. Reduced expression of hPMS2 provides a growth advantage and stimulates proliferation which encourages malignant transformation in non-cirrhotic HCV-infected patients via acquisition of more genetic damages. | Abdel-Rahman N. Zekri Gelane M. Sabry Abeer A. Bahnassy Kamal A. Shalaby Sabrin A.Abdel-Wahabh Serag Zakaria | 2005 | World Journal of Gastroenterology2005，11，20： | 10 |
| 4 | Circulating tumor and cancer stem cells in hepatitis C virusassociated liver disease显示文摘AIM:To assess the role of circulating tumor cells(CTCs)and cancer stem cells(CSCs)in hepatitis C virus(HCV)-associated liver disease.METHODS:Blood and/or tissue samples were obtained from HCV(genotype 4)-associated hepatocellularcarcinoma patients(HCC;n=120),chronic hepatitis C patients(CH;n=30)and 33 normal control subjects(n=33).Serum levels of alpha-fetoprotein(AFP),alkaline phosphatase,and alanine and aspartate aminotransferases were measured.Cytokeratin 19(CK19)monoclonal antibody was used to enumerate CTCs,and CD133 and CD90 were used to enumerate CSCs by flow cytometry.The expression levels of the CSCs markers(CD133 and CD90)as well as telomerase,melanoma antigen encoding gene 1(MAGE1)and MAGE3 were assessed by RTPCR and quantitative real-time polymerase chain reactions.The number of CTCs and/or the expression levels of CK19,CD133,telomerase,MAGE1 and MAGE3 were correlated to the standard clinicopathologic prognostic factors and disease progression.RESULTS:Levels of AFP,alkaline phosphatase and aspartate aminotransferase were significantly different among the HCC,CH and control groups(P<0.001),whereas alanine aminotransferase differed significantly between patient(HCC and CH)and control groups(P<0.001).At the specified cutoff values determine by flow cytometry,CK19(49.8),CD90(400)and CD133(73)were significantly higher in the blood of HCC patients compared to those in the CH and control groups(P<0.001).On the other hand,CD133 at a 69.5 cutoff was significantly higher in the CH compared to the control group(P≤0.001).Telomerase,MAGE1 and MAGE3RNA were expressed in 55.71%,60.00%and 62.86%of the HCC patients,respectively,but were not detected in patients in the CH or control groups,which were statistically significant(Ps<0.001).The expression levels of telomerase,CD90,MAGE3,CD133 and CK19 were all significantly associated with high tumor grade and advanced stage in HCC patients(all P s<0.05).CONCLUSION:CTC counts and AFP,CK19,telomerase,and MAGE1/MAGE3 expression predict disease progression in patients with HCV,whereas telomerase,MAGE3,CD90,CD133 and CK19 are prognostic mark- | Abeer A Bahnassy Abdel-Rahman N Zekri Ahmed El-Bastawisy Amal Fawzy Marwa Shetta Nehal Hussein Dalia Omran Abdallah A S Ahmed Samir S El-Labbody | 2014 | World Journal of Gastroenterology2014，20，48： | 9 |
| 5 | Role of relevant immune-modulators and cytokines in hepatocellular carcinoma and premalignant hepatic lesions显示文摘AIM To assess the levels of different immune modulators in patients with hepatocellular carcinoma(HCC),in relation to other hepatic diseases.METHODS Eighty-eight patients were included in the current study and represented patients with HCC(20),liver cirrhosis(28) and chronic hepatitis(CH;25),and normal controls(NC;15).Peripheral blood was isolated for immunophenotyping of active myeloid dendritic cells(m DCs;CD1 c and CD40),mature inactive myeloid cells(CD1 c and HLA),active plasmacytoid cells(p DCs;CD303 and CD40),mature inactive p DCs(CD30 and HLA),active natural killer(NK) cells(CD56 and CD161),active NK cells(CD56 and CD314) and inactive NK cells(CD56 and CD158) was done by flow cytometry.Serum levels of interleukin(IL)-2,IL-10,IL-12,IL-1β,interferon(IFN)-α,IFN-γ and tumor necrosis factor(TNF)-αR2 were assessed by ELISA.RESULTS Active m DCs(CD1 C+/CD40+) and inactive m DCs(CD1 c+/HLA+) were significantly decreased in HCC patients in relation to NC(P < 0.001).CD40+ expression on active p DCs was decreased in HCC patients(P < 0.001),and its level was not significantly changed among other groups.Inactive p DCs(CD303+/HLA+),inactive NKs(CD56+/CD158+) and active NKs(CD56+/CD161+) were not statistically changed among the four groups studied;however,the latter was increased in CH(P < 0.05).NKG2 D was statistically decreased in HCC,CH and cirrhosis(P < 0.001),and it was not expressed in 63%(12/20) of HCC patients.There was significant decrease of IL-2,IFN-α and IFN-γ(P < 0.001),and a significant increase in IL-10,IL-1β,and TNF-αR2(P <0.01,P < 0.001 and P < 0.001;respectively) in HCC patients.There was inverted correlation between IL-12 and IL-1β in HCC(r =-0.565,P < 0.01),with a strong correlation between p DCs(CD303+/CD40+) and NKs(CD56+/CD161+;r = 0.512,P < 0.05) as well as inactive m DCs(CD1 c+/HLA+) and inactive NK cells(CD56+/CD158+;r = 0.945,P < 0.001).CONCLUSION NKG2 D,CD40,IL-2 and IL-10 are important modulators in the development and progression of HCC. | Abdel-Rahman N Zekri Somaya El Deeb Abeer A Bahnassy Abeer M Badr Mona S Abdellateif Gamal Esmat Hosny Salama Marwa Mohanad Ahmed Esam El-dien Shimaa Rabah Assmaa Abd Elkader | 2018 | World Journal of Gastroenterology2018，24，11： | 6 |
| 6 | Cytokine profile in Egyptian hepatitis C virus genotype-4 in relation to liver disease progression显示文摘AIM: To observe the imbalance between T helper cell Th1 and Th2 cytokines in several chronic hepatitis disease at different stages of disease progression.METHODS: We measured the cytokine levels of Th1 (IL-2 and IL-2R), Th2 (IL-10) and the pro-inflammatory cytokines (IL-6 and IL-6R and TNF and TNF-RⅠ and Ⅱ)by the ELISA technique in the sera of 33 hepatocellular carcinoma (HCC) patients and 20 chronic liver disease (CLD) patients. In addition, 20 asymptomatic hepatitis C virus carriers and 20 healthy subjects negative for hepatitis C virus(HCV) markers served as controls.RESULTS: Anti-HCV antibodies were found to be positive in 94% of HCC cases and 75% of CLD cases.On the other hand, HCV viremia was detected using RTPCR in 67% of HCC cases and 65% of CLD cases. HBsAg was positive in 9% of HCC cases and 30% of CLD cases.Also bilharzial-Ab was positive in 55% of HCC cases,65% of CLD cases and in 70% of asymptomatic carriers (ASC). HCC patients had significantly higher values of IL-2R, TNF-RⅡ (P<0.001), and TNF-RⅠ (P>0.05), but lower TNFα (P<0.001) and IL-6 (P = 0.032) in comparison to ASC. But, in comparison to non-cancer controls, HCC patients had higher values of IL-2R, IL-6R, TNF-RⅠ and TNF-RⅡ, but lower TNF-α (P<0.001). CLD patients had higher IL-2R, TNF-RⅠ, and TNF-RⅡ (P<0.001) than ASC. But, in comparison to non-cancer controls, CLD patients had higher values of IL-2R, TNF-RⅠ and TNF-RⅡ, but lower TNF-α (P<0.001). IL-10 was higher (though not significantly) in HCC and CLD patients than in symptomatic carriers and non-cancer controls.CONCLUSION: Liver disease progression from CLD to HCC due to HCV genotype-4 infection is associated with an imbalance between Th1 and Th2 cytokines. IL-2R,TNF-RⅠ, and TNF-RⅡ could be used as potential markers. | Abdel-Rahman N Zekri Mohammed S El-Din Ashour Ahmed Hassan Hanaa M Alam El-Din Amal MR El-Shehaby Maha A Abu-Shady | 2005 | World Journal of Gastroenterology2005，11，42： | 4 |
| 7 | The inhibitory effects on adult male reproductive functions of crude garlic (Allium sativum) feeding显示文摘瞄准:在成年雄的老鼠上调查粗略的大蒜的效果繁殖功能。方法:三十只雄的老鼠被划分成五个组:组 1 (未经治疗) 并且组 2, 3, 4 和 5 分别地用 5% , 10% , 15% 和 30% 粗略的大蒜被喂 30 天。睾丸和附件机关被称,一些标记被估计。光和电子显微镜学观察也被执行。结果:重要减少在组的体重被观察 4 (14% ;P <
0.01 ) 并且 5 (20% ;P <
0.01 ) ;在组 5 的前列腺重量(29.1% ;P <
0.05 ) 并且在组的精囊重量 3 (14.4% ;P <
0.01 ) , 4 (18.3% ;P <
0.01 ) 并且 5 (27.3% ;P <
0.01 ) 。相反,睾丸和附睾重量是未改变的。在附睾织物,高山哈糖甙酶活动和精子密度是未改变的。处理在组在睾丸激素浆液层次导致了重要减少 3 (77.3% ;P <
0.01 ) , 4 (77.3% ;P <
0.01 ) 并且 5 (90.9% ;P <
0.01 ) ,与 LH 浆液层次的重要增加联系了(P <
0.01 ) 。阴囊的组织学在空细精管的百分比显示出剂量依赖者增加。而且,阴囊的功能被影响;在磷酸酶酸活动的重要减少(P <
0.01 ) 并且睾丸激素(P <
0.05 ) 内容被观察。结论:在 1 个月期间,在 10% , 15% 和 30% 点的减少的睾丸激素分泌物和改变的精子发生开的粗略的大蒜消费。 | Irnen Hammami Afef Nahdi Claire Mauduit Mohamed Benahmed Mohamed Amri Awatef Ben Amar Semy Zekri Ahmed El May Michele Veronique El May | 2008 | Asian Journal of Andrology2008，10，4： | 4 |
| 8 | Expression of insulin-like growth factor-II, matrix metalloproteinases, and their tissue inhibitors as predictive markers in the peripheral blood of HCC patients显示文摘 | H.M. El Tayebi W. Salah I.H. El Sayed A.R.N. Zekri N. Zayed E.S. Salem G. Esmat A.I. Abdelaziz | 2011 | Biomarkers2011，，4： | 2 |
| 9 | Early detection of hepatocellular carcinoma co-occurring with hepatitis C virus infection: A mathematical model显示文摘AIM: To develop a mathematical model for the early detection of hepatocellular carcinoma(HCC) with a panel of serum proteins in combination with α-fetoprotein(AFP).METHODS: Serum levels of interleukin(IL)-8, soluble intercellular adhesion molecule-1(s ICAM-1), soluble tumor necrosis factor receptor Ⅱ(s TNF-RⅡ), proteasome, and β-catenin were measured in 479 subjects categorized into four groups:(1) HCC concurrent with hepatitis C virus(HCV) infection(n = 192);(2) HCV related liver cirrhosis(LC)(n = 96);(3) Chronic hepatitis C(CHC)(n = 96); and(4) Healthy controls(n = 95). The R package and different modules for binary and multi-class classifiers based on generalized linear models were used to model the data. Predictive power was used to evaluate the performance of the model. Receiver operating characteristic curve analysis over pairs of groups was used to identify the best cutoffs differentiating the different groups. RESULTS: We revealed mathematical models, based on a binary classifier, made up of a unique panel of serum proteins that improved the individual performance of AFP in discriminating HCC patients from patients with chronic liver disease either with or without cirrhosis. We discriminated the HCC group from the cirrhotic liver group using a mathematical model(-11.3 + 7.38 × Prot + 0.00108 × s ICAM + 0.2574 ×β-catenin + 0.01597 × AFP) with a cutoff of 0.6552, which achieved 98.8% specificity and 89.1% sensitivity. For the discrimination of the HCC group from the CHC group, we used a mathematical model [-10.40 + 1.416 × proteasome + 0.002024 × IL + 0.004096 × s ICAM-1 +(4.251 × 10-4) × s TNF + 0.02567 ×β-catenin + 0.02442 × AFP] with a cutoff 0.744 and achieved 96.8% specificity and 89.7% sensitivity. Additionally, we derived an algorithm, based on a binary classifier, for resolving the multi-class classification problem by using three successive mathematical model predictions of liver disease status. CONCLUSION: Our proposed mathematical model may be a useful method for the early detection of different statuses of liver disease co-occurring with HCV infection. | Abdel-Rahman Nabawy Zekri Amira Salah El-Din Youssef Yasser Mabrouk Bakr Reham Mohamed Gabr Ola Sayed Ahmed Mostafa Hamed Elberry Ahmed Mahmoud Mayla Mohamed Abouelhoda Abeer A Bahnassy | 2016 | World Journal of Gastroenterology2016，22，16： | 2 |
| 10 | End-of-life care of persons with dementia显示文摘 | Michel JP Pautex S Zekry D | 2002 | J Gerontol A Biol Sci Med Sci2002，57，10： | 1 |
| 11 | Fuzzy wavelet neural network with an accelerated hybrid learning algorithm 显示文摘 | DAVANIPOOR M ZEKRI M SHEIHOLESLAM F | 2012 | IEEE Trans Fuzzy Sys2012，20，3： | 1 |
| 12 | Concordance of DNA methylation pattern in plasma and tumor DNA of Egyptian hepatocellular carcinoma patients显示文摘 | Iyer P Zekri AR Hung CW | | 0，，01： | 1 |
| 13 | Real-time reverse transcriptase- polymerase chain reaction (RT-PCR) for measurement of cytokine and growth factor mRNA expression with fluorogenic probes or SYBR Green Ⅰ显示文摘 | Yin JL Shackel NA Zekry A | 2001 | Immunol Cell Biol2001，79，3： | 1 |
| 14 | Markers of bone turnover for the management of patients with bone metastases from prostate cancer显示文摘 | GARNERO P BUCHS N ZEKRI J | 2000 | Br J Cancer2000，82，4： | 1 |
| 15 | Cyclin A and Cyclin D1 as Significant Prognostic Markers in Colorectal Cancer Patients显示文摘 | Bahnassy A A Zekri A R EI Houssini S | 2004 | BMC Gastroenterol2004，23，4： | 1 |
| 16 | Markers of bone turnover for the management of patients with bone metastases from prostate cancer显示文摘 | Buchs N Zekri J | 2000 | Br J Cancer2000，82，4： | 1 |
| 17 | Frailty Syndrome: A Transitional State in a Dynamic Process显示文摘 | Lang Pierre-olivier Michel Jean-pierre Zekry Dina | 2009 | Gerontology2009，，5： | 1 |
| 18 | The vascular lesions in vascular and mixed dementia:the weight of functional neuroanatomy显示文摘 | Zekry D Duyckaerts C Belmin J | 2003 | Neurobiol Aging2003，24，2： | 1 |
| 19 | The role of cyclins and cyclins inhibitors in the multistep process of HPV-associated cervical carcinoma 显示文摘 | Bahnassy AA Zekri AR Alam El-Din HM | 2006 | J Egypt Natl Cane Inst2006，18，4： | 1 |
| 20 | Ehanced recov-ery of medium crude oil in heterogeneous reservoirs us-ing air injection/in situ combustion-horizontal well tech-nology 显示文摘 | Al-Honi M Greaves M ZeKri A Y | 2002 | Petroleum Science and Technology2002，20，56： | 1 |
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