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| 1 | Antidiabetic Effects of Gegen Qinlian Decoction via the Gut Microbiota Are Attributable to Its Key Ingredient Berberine显示文摘Gegen Qinlian Decoction(GQD),a traditional Chinese medicine(TCM)formula,has long been used for the treatment of common metabolic diseases,including type 2 diabetes mellitus.However,the main limitation of its wider application is ingredient complexity of this formula.Thus,it is critically important to identify the major active ingredients of GQD and to illustrate mechanisms underlying its action.Here,we compared the effects of GQD and berberine,a hypothetical key active pharmaceutical ingredient of GQD,on a diabetic rat model by comprehensive analyses of gut microbiota,short-chain fatty acids,proinflammatory cytokines,and ileum transcriptomics.Our results show that berberine and GQD had similar effects on lowering blood glucose levels,modulating gut microbiota,inducing ileal gene expression,as well as relieving systemic and local inflammation.As expected,both berberine and GQD treatment significantly altered the overall gut microbiota structure and enriched many butyrate-producing bacteria,including Faecalibacterium and Roseburia,thereby attenuating intestinal inflammation and lowering glucose.Levels of short-chain fatty acids in rat feces were also significantly elevated after treatment with berberine or GQD.Moreover,concentration of serum proinflammatory cytokines and expression of immune-related genes,including Nfkb1,Stat1,and Ifnrg1,in pancreatic islets were significantly reduced after treatment.Our study demonstrates that the main effects of GQD can be attributed to berberine via modulating gut microbiota.The strategy employed would facilitate further standardization and widespread application of TCM in many diseases. | Xizhan Xu Zezheng Gao Fuquan Yang Yingying Yang Liang Chen Lin Han Na Zhao Jiayue Xu Xinmiao Wang Yue Ma Lian Shu Xiaoxi Hu Na Lyu Yuanlong Pan Baoli Zhu Linhua Zhao Xiaolin Tong Jun Wang | 2020 | Genomics, Proteomics & Bioinformatics2020，18，6： | 29 |
| 2 | State-target strategy:a bridge for the integration of Chinese and Western Medicine显示文摘The methods of Traditional Chinese Medicine(TCM)’s diagnosis and treatment have undergone several changes.It is crucial to build a proper model which is capable to modernize TCM into a both standardized and individualized treatment.Tong xiao-lin proposed the state-target strategy to build a bridge for the integration of Chinese and Western medicine.It is a model based on modern medical disease concepts and using the method of TCM to balance the pathological states and adopting the achievements of pharmacology of Chinese medicine to focus on the disease targets,symptom targets,and biochemical indicator targets.The reconstruction of TCM diagnosis and treatment system for diabetes is a good example to demonstrate this theory.It could improve the clinical efficacy,support the scientific research,and reinforce the standardization of TCM. | GOU Xiaowen GAO Zezheng YANG Yingying LI Qingwei TONG Xiaolin CHEN Keyu LEI Ye SONG Bin ZHAO Linhua | 2021 | Journal of Traditional Chinese Medicine2021，41，1： | 3 |
| 3 | Genetic Variation in the Endangered Endemic Species Cycas fairylakea (Cycadaceae) in China and Implications for Conservation显示文摘 | Shuguang Jian Yang Zhong Nian Liu Zezheng Gao Qiang Wei Zhenhua Xie Hai Ren | 2006 | Biodiversity and Conservation2006，，5： | 1 |
| 4 | Genetic Variation in the Endangered Endemic Species Cycas fairylakea (Cycadaceae) in China and Implications for Conservation显示文摘 | Shuguang Jian Yang Zhong Nian Liu Zezheng Gao Qiang Wei Zhenhua Xie Hai Ren | 2006 | Biodiversity and Conservation2006，，5： | 1 |
| 5 | Biological characteristics of wild Cycas fairylakea population in Guangdong Province,China显示文摘There are five wild populations of Cycas fairylakea in Guangdong Province,China,three of which are newly found.A study of the biological characteristics of C.fairylakea populations showed that this species had a narrow colonization area within 300 hm^(2),and an island pattern of distribution.Because of the overexploitation,urbanization,environment pollution,plant diseases,and insect pests,the wild populations and individuals of C.fairylakea decreased markedly in the past decades.All five populations have an opposite pyramid age structure,few coning plants,few seed production,and low level of seed germination rate or sterility.In conclusion,C.fairylakea in Guangdong Province was threatened seriously and an urgent need to take effective efforts to protect the plants and habitats in its location sites was required. | JIAN Shuguang LIU Nian GAO Zezheng WEI Qiang XIE Zhenhua WU Mei REN Hai | 2006 | Frontiers in Biology2006，1，4： | 0 |
| 6 | Structure-guided engineering of transcriptional activator XYR1 for inducer-free production of lignocellulolytic enzymes in Trichoderma reesei显示文摘The filamentous fungus Trichoderma reesei is widely used for the production of lignocellulolytic enzymes in industry.XYR1 is the major transcriptional activator of cellulases and hemicellulases in T.reesei.However,rational engineering of XYR1 for improved lignocellulolytic enzymes production has been limited by the lack of structure information.Here,alanine 873 was identified as a new potential target for the engineering of XYR1 based on its structure predicted by AlphaFold2.The mutation of this residue to tyrosine enabled significantly enhanced production of xylanolytic enzymes in the medium with cellulose as the carbon source.Moreover,xylanase and cellulase production increased by 56.7-and 3.3-fold,respectively,when glucose was used as the sole carbon source.Under both conditions,the improvements of lignocellulolytic enzyme production were higher than those in the previously reported V821F mutant.With the enriched hemicellulases and cellulases,the crude enzymes secreted by the A873Y mutant strain produced 51%more glucose and 52%more xylose from pretreated corn stover than those of the parent strain.The results provide a novel strategy for engineering the lignocellulolytic enzyme-producing capacity of T.reesei,and would be helpful for understanding the molecular mechanisms of XYR1 regulation. | Qinqin Zhao Zezheng Yang Ziyang Xiao Zheng Zhang Jing Xing Huiqi Liang Liwei Gao Jian Zhao Yinbo Qu Guodong Liu | 2023 | Synthetic and Systems Biotechnology2023，8，4： | 0 |
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