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| 1 | Over-expression of heat shock protein 27 attenuates doxorubicin-induced cardiac dysfunction in mice显示文摘 | Liu,L Zhang,X Qian,B Min,XY Gao,X Li,CF Cheng,YL Huang,J | 2007 | 南京医科大学学报(自然科学版)2007，27，11： | 15 |
| 2 | Dietary factors and smoking as risk factors for PD in a rural population in China: a nested case-control study显示文摘 | Ma L Zhang L Gao XH Chen W Wu YP Wang Y Li XF Como P Kieburtz K Qiao YL | 2006 | 中国生物学文摘2006，20，8： | 13 |
| 3 | Tumor growth inhibition effect of hIL-6 on colon cancer cells transfected with the target gene by retroviral vector显示文摘AIM To observe the tumor inhibitory effects bytransfecting IL-6 cDNA into colon cancer cell lineHT-29 with retroviral vector pZIP cDNA.METHODS Human IL-6 gene was reconstructedin retrovirus vector and transfected into incasingcells PA317 by lipofectamine mediated method,the clones of the cells transferred with hlL-6were selected by G418,and targeted HT-29 cellswere infected with the virus granules secretedfrom PA317 and also selected by G418.Test genetranscription and expression level byhybridization,ELISA and MTT assay,etc.Analyze tumor inhibitory effects according to thecell growth curve,plating forming rate andtumorigenicity in nude mice.RESULT Successfully constructed andtransfected recombinant expressing vectorspZIPIL-6 cDNA and got positive transfected celllines.The colon cancer cell line(HT-29 IL-5)transfected with the hlL-5 gene by retroviralvector was established.The log proliferationperiod and the doubling time of this cell line wasbetween 4 to 7 days and 2.5 days according tothe direct cell count,the cell proliferation wasobviously inhibited with MTT assay,the platinginhibitory rate was 50% by plating efficiencytest.When HT-29 IL-6 cells were inoculated intothe nude mice subcutaneously,carcinogenicactivity of the solid tumor was found superior tothe control group and the size of tumor was notsignificantly enlarged.Injection of combinationvirus fluid containing 11.-6 gene intotransplantation tumors could inhibit the growthand development of the tumor.CONCLUSION IL-6 could inhibit the growth andproliferation of colon cancer cells by retroviralvector-mediated transduction. | Xiao B Jing B Zhang YL Zhou DY Zhang WD | 2000 | World Journal of Gastroenterology2000，6，1： | 11 |
| 4 | A long-term follow-up study on hepatitis B surface antigen-positive patients undergoing allogeneic hematopoietic stem cell transplantation显示文摘 | Hui CK Lie A Au WY Leung YH Ma SY Cheung WW Zhang HY Chim CS Kwong YL Liang R Lau GK | 2006 | 中国生物学文摘2006，20，5： | 10 |
| 5 | Effects of partial portal vein arterialization on the hilar bile duct in a rat model显示文摘BACKGROUND: Liver revascularization is frequently required during the enlarged radical operation for hilar cholangio carcinoma involving the hepatic artery. Researchers have carried out a number of experiments applying partial porta vein arterialization (PVA) in clinical practice. In this study we aimed to establish a theoretical basis for clinical application o partial PVA and to investigate the effects of partial PVA on ra hilar bile duct and hepatic functions. METHODS: Thirty rats were randomly and equally assigned into 3 groups: control (group A), hepatic artery ligation+bile duct recanalization (group B), and partial PVA+bile duc recanalization (group C). Proliferation and apoptosis o rat hilar bile duct epithelial cells, arteriolar counts of the peribiliary plexus (PBP) of the bile duct wall, changes in serum biochemistry, and pathologic changes in the bile duc were assessed 1 month after operation. RESULTS: The proliferation of hilar bile duct epithelial cells in group B was greater than in groups A and C (P<0.01). No apoptotic hilar bile duct epithelial cells were detected in any of the groups. The PBP arteriolar counts of the hilar bile duc wall were similar in groups A and C (P>0.05), but the coun was lower in group B than in group A (P<0.01). No statistically significant differences in alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase and albumin were found in the 3 groups. The gamma-glutamyltransferase value was higher in group B than in groups A and C (P<0.01) The hepatic tissues of groups A and C showed no significan abnormality. Chronic inflammatory changes in the hilar bile duct walls were observed only in group B. CONCLUSION: Partial PVA can restore the arterial blood supply of the hilar bile duct and significantly extenuate the injury to hilar bile duct epithelial cells resulting from hepatic artery ligation. | Shao-Hua Guo, Chong-Hui Li,Yong-Liang Chen, Jian-Ning Song,Ai-Qun Zhang and Cheng Zhou Department of Hepatobiliary Surgery (Guo SH, Li CH, Chen YL, Song JN, Zhang AQ and Zhou C) and Department of Surgical Oncology (Guo SH), General Hospital of PLA, Beijing 100853, China | 2011 | Hepatobiliary & Pancreatic Diseases International2011，10，5： | 8 |
| 6 | Expression of co-stimulator 4-1BB molecule in hepatocellular carcinoma and adjacent non-tumor liver tissue,and its possible role in tumor immunity显示文摘AIM:To investigate the expression of 4-1BB molecule in hepatocellular carcinoma (HCC) and its adjacent tissues.METHODS:Reverse transcription-polymerase chain reaction (RT-PCR) was used to determine the gene expression of 4-1BB in hepatocarcinoma and its adjacent tissues,and peripheral blood mononuclear cells (PBMCs) from both HCC and health control groups.Flow cytometry was used to analyse the phenotypes of T cell subsets from the blood of HCC patients and healthy volunteers,and further to determine whether 4-1BB molecules were also expressed on the surface of CD4+ and CD8+ T cells. The localization of 4-1BB proteins on tumor infiltrating T cells was determined by direct immunofluorescence cytochemical staining and detected by confocal microscopy.RESULTS:4-1BB mRNA, which was not detectable in normal liver,was found in 19 liver tissues adjacent to tumor edge(<1.0cm).Low expression of 4-1BB mRNA was shown in 8 tumor tissues and 6 liver tissues located within 1 to 5cm away from tumor edge. In PBMCs, 4-1BB mRNA was almost not detected. Percentage of CD4^+, CD8^+ and CD3^+/CD25^+ T cells, as well as ratio of CD4 to CD8 revealed no difference between groups (P>0.05,respectively),while a significant lower percentage of CD3^+ T cell was found in HCC group as compared to healthy control group (P<0.05).However, 4-1BB molecules were almost not found on the surface of CD4+ and CD8+ T cells in HCC and healthy control group.Double-staining of 4-1BB^+/CD4^+ and 4-1BB^+/CD8^+ immunofluorescence on tumor infiltrating T cells was detected in 13 liver tissues adjacent to tumor edge (<1.0cm) by confocal microscopy.CONCLUSION: Although HCC may escape from immune attack by weak immunogenicity or downregulated expression of MHC-1 molecules on the tumor cell surface,tumor infiltrating T cells can be activated via other costimulatory signal pathways to exert a limited antitumor effect on local microenvironment. The present study also implicates that modulating 4-1BB/4-1BBL costimulatory pathway may be an effective immunotherapy strategy to augment the host response. | Wan, YL Zheng, SS Zhao, ZC Li, MW Jia, CK Zhang, H | 2004 | World Journal of Gastroenterology2004，10，2： | 6 |
| 7 | 脂蛋白a水平与冠状动脉粥样硬化性心脏病患者复发事件的关系显示文摘脂蛋白a是否是冠状动脉粥样硬化性心脏病(冠心病)患者复发性心血管事件(recurrent cardiovascular events,RCVEs)的预测因子尚未确定。本研究旨在探讨脂蛋白a对一个真实世界、大规模首诊心血管事件(cardiovascular event,CVE)患者队列中RCVEs的潜在影响。方法:本实验为多中心的前瞻性研究,纳入经血管造影诊断的冠心病并经历首次CVE的患者7562例。入院时测量所有患者的脂蛋白a浓度. | 周卫(译) 叶鹏(校) Liu HH Cao YX Jin JL Zhang HW Hua Q Li YF Guo YL Zhu CG Wu NQ Gao Y Xu RX Hong LF | 2020 | 中华高血压杂志2020，28，5： | 5 |
| 8 | Discovery of virus pathogen from soft-shelled turtle Trionyx sinesis显示文摘THE soft-shelled turtle Trionyx sinesis, because of its nutritional and medicinal values, | Zhang, QY Li, ZQ Jiang, YL Liang, SC Gui, JF | 1997 | Chinese Science Bulletin1997，42，6： | 3 |
| 9 | Static Adsorption of Xenon on ACF at 257 K显示文摘The static adsorption of xenon on active carbon fiber (ACF) at 257 K was measured with ASAP2010 specific surface area and pore diameter distribution instrument by changing the working gas from nitrogen to xenon. Compared with grain activated carbon (GAC), the results were as follows: (1) The adsorption performance of Viscose-based ACF (VACF-As) was the best among all absorbents tested. VACF-A3 was the superior xenon absorbent. The performance of pitch-based ACF (PACF-Cs) approached that of GAC, (2) Due to the difference of aperture distribution, the adsorption performances of ACF with different radics were different under the same experiment conditions even though the specific surface area was similar, (3) There were some differences of adsorptive capacity among ACF absorbents which had the same radic, however there was not definite relationship between their specific surface area and adsorptive capacity, (4) The adsorption of xenon on all kinds of ACF agrees with Langmuir equation, (5) The adsorptive curves can be fitted with a binomial equation. | Zhang, HT Wang, YL Wang, XH Zhang, LX Chen, LY | 2002 | Journal of Materials Science & Technology2002，18，6： | 3 |
| 10 | Expression of gap junctions and inhibition of cell growth in communication-defected cells after transfection with gap junction gene Cx43 cDNA显示文摘Gap junctions form intercellular channels for direct exchange of metabolites and second messengers between adjacent cells. The existence of a multi-gene family encoding gap junction proteins, i. e. the connexins (Cx), has been recently demonstrated. Despite the overall sequence homologies and a similar predicted structural motif, their functional properties differ in various tissues. Because of coexpression of two or more connexin proteins in one tissue, determination of properties specific for each connexin is complicated. The regulation of expression of gap junctions and their roles in cellular activities remain mostly unelucidative. Controlled manipulation of connexin molecules in Xenopus oocyte expression system has been use- | Zhang, ZQ Lin, ZX Wang, NQ Han, YL | 1997 | Chinese Science Bulletin1997，42，5： | 3 |
| 11 | Effect of polysaccharides of cassiae seeds on the intestinal microflora of piglets显示文摘 | Deng ZY Zhang JW Li J Fan YW Cao SW Huang RL Yin YL Yin, Yu-long Zhong HY Li TJ | 2007 | 中国生物学文摘2007，21，6： | 3 |
| 12 | Polymorphisms in DNA base excision repair genes ADPRT and XRCC1 and risk of lung cancer显示文摘 | Zhang XM Miao XP Liang G Hao BT Wang YG Tan W Li Y Guo YL He FC Wei QY Lin DX | 2006 | 中国生物学文摘2006，20，4： | 2 |
| 13 | Improved cryopreservation of human embryonic stem cells with trehalose显示文摘 | Wu CF Tsung HC Zhang W J Wang Y Lu JH Tang ZY Kuang YP Jin W Cui L Liu W Cao YL | 2006 | 中国生物学文摘2006，20，7： | 2 |
| 14 | Targeting small arteries of hypertensive status with novel ATP-sensitive potassium channel openers显示文摘 | Wang H Zhang YL Chen YP | 2005 | Curr Vasc Pharmacol2005，3，2： | 1 |
| 15 | Reconstruction of chemically burned rat corneal surface by bone marrow-derived human mesenchymal stem cells 显示文摘 | Ma YL Xu YS Xiao ZF Yang W Zhang C Song E | 2005 | Stem Cells2005，24，2： | 1 |
| 16 | Regulation of the type I protein secretion system by the MisR/MisS two-component system in Neisseria meningitides 显示文摘 | Sannigrahi S Zhang X Tzeng YL | 2009 | Microbiology2009，155，5： | 1 |
| 17 | Protection rrom ischemic heart injury by a vigilant heine oxygenase-1 plasmid system 显示文摘 | Tang YL Tang Y Zhang YC | 2004 | Hypertension2004，43，4： | 1 |
| 18 | Clinical efficacy of a308 nm excimer laser in the treatment of vitiligo显示文摘 | ZHANG XY HE YL DONG J et a1 | 2010 | Photoder-matol Photoimmunol Photomed2010，26，3： | 1 |
| 19 | Secondary metabolites from the stem bark of Juglans mandshuriea 显示文摘 | Lin H Zhang YW Bao YL | 2013 | Biochem Syst Ecol2013，51，： | 1 |
| 20 | Molecular cloning and functional characterization of porcine toll - like receptor 7 involved in recognition of single - stranded RNA virus/ssRNA 显示文摘 | Zhang YL Guo YJ Sun SH | 2008 | Mol lnununol2008，45，4： | 1 |
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