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| 1 | Down-regulation of Hsp90 could change cell cycle distribution and increase drug sensitivity of tumor cells显示文摘:AIM To construct Hsp90 antisense RNAeukaryotic expression vector, transfect it intoSGC7901 and SGC7901/VCR of MDR-type humangastric cancer cell lines, HCC7402 of humanhepatic cancer and Eel09 of human esophagealcancer cell lines, and to study the cell cycledistribution of the gene transected cells andtheir response to chemotherapeutic drugs.METHODS A I .03kb cDNA sequence of Hsp90Pwas obtained from the primary plasmid phHsp90by EcoR 1 and BamH I nuclease digestion andwas cloned to the EcoR 1 and BamH 1 site ofthe pcDNA by T4DNA ligase and an antisenseorientation of Hsp900 expression vector wasconstructed. The constructs were transfectedwith lipofectamine and positive clones wereselected with G418. The expression of RNA wasdetermined with dot blotting and RNaseprotection assay, and the expression of Hsp90protein determined with Western blot. Cell cycledistribution of the transfectants was analyzedwith flow cytometry, and the drug sensitivity ofthe transfectants to adriamycin (ADR ),vincrinstine (VCR ), mitomycin (MMC ) andcyclophosphamide (CTX ) with MTT andintracellular drug concentration of thetransfectants was determined with flowcytometry.RESULTS In EcoR 1 and BamH I restrictionanalysis, the size and the direction of the clonedsequence of Hsp900 remained what had beendesigned and the gene constructs were namedpcDNA-Hsp90. AH^SGC7901, AH^SGC7901/ VCR,AH-HCC7402 and AH-Eel09 cell clones allexpressed Hsp90 anti--sense RNA. Theexpression of Hsp90 was down--regulated in AHSGC7901, AH--SGC7901/ VCR, AH-HCC7402 andAH--Eel09 cell clones. Cell cycle distribution waschanged differently. In AH-SGC7901/ VCR andAH-Ec109 cells, G, phase cells were increased; Sphase and G, phase cells were decreased ascompared with their parental cell lines. In AHSGC7901 cell, G, phase cells were decreased, Qphase cells increased and S phase cells were notchanged, and in AH-HCC7402 cells G,, S and qphase cells remained unchanged as comparedwith their parental cell lines. The sensitivity ofAH--SGC7901, AH--SGC7901/ VCR, AH-HCC7402 andAH-Ec109 to chemotherapeutic drugs, thesensitivity ot AH--SGC7901/ VCR to ADR, VCR,MMC and CTX the sensitivity of AH-HCC7402 toADR and VCR, and the sensitivity of Eel09 toADR, VCR and CTX all increased as comparedwith their parental cell lines. The meanfluorescence intensity of ADR in AH--SGC7901,AH-SGC7901/ VCR, AH--HCC7402 and AH-Ec109was also significantly elevated (P< 0. 05).CONCLUSION Down-regulation of HsP90 couldchange cell cycle distribution and increase thedrug sensitivity of tumor cells. | Liu XL Xiao B Yu ZC Guo JC Zhao QC Xu L Shi YQ Fan DM | 1999 | World Journal of Gastroenterology1999，5，3： | 21 |
| 2 | 在有类型 2 糖尿病的病人的 nephropathy 的管理。 | Critchley JA Zhao HL Tomlinson B Leung W Thomas GN Chan JC Cockram CS | 2002 | Chinese Medical Journal2002，，1： | 12 |
| 3 | TLR signaling by tumor and immune cells: a double edged sword 显示文摘 | Huang B Zhao J Unkeless JC | 2008 | Oncogeue2008，27，2： | 1 |
| 4 | Advances in the genetics of anti - glomerular basement membrane disease 显示文摘 | Zhou XJ Lv JC Zhao MH | 2010 | Am J Neph- rol2010，32，5： | 1 |
| 5 | Transsphenoidal micro- surgery显示文摘 | Ciric I Rosenblatt S Zhao JC | 2002 | Neurosurgery2002，51，1： | 1 |
| 6 | TLR signaling by tumor and immune cells:a double edged sword显示文摘 | Huang B Zhao J Unkeless JC | 2008 | Oncogene2008，27，2： | 1 |
| 7 | Transsphenoidal microsurgery显示文摘 | Ciric I Rosenblatt S Zhao JC | 2002 | Neurosurgery2002，51，1： | 1 |
| 8 | An ecological study on dynamics of toxic Microcystis blooms in a eutrophic park pond, Wuhan, China | Cheng, K Zhao, YJ Xu, M Yin, J Xiong, Q Zhou, JC Wang, YZ Zhang, W Wang, JW Liu, ZY Wang, FR | 2002 | Progress in Natural Science:Materials International2002，12，1： | 1 |
| 9 | Polyhydroxyalkanoate(PHA)scaffolds with good mechanical properties and biocompatibility显示文摘 | Zhao K Deng Y Chen JC | | 0，，06： | 1 |
| 10 | TLR signaling by tumor and immune cells:a double-edged sword显示文摘 | Huang B Zhao J Unkeless JC | 2008 | Oncogene2008，27，2： | 1 |
| 11 | TLR signaling by tumor and immune cells: a double-edged sword 显示文摘 | Huang B Zhao J Unkeless JC | 2008 | Oncogene2008，27，： | 1 |
| 12 | Transsphenoidal microsurgery显示文摘 | Ciric I Rosenblatt S Zhao JC | 2002 | Neurosurgery2002，51，1： | 1 |
| 13 | Suboccipical retrosi gmoidal approach for removal of vesti bular schwannomas:Facial nerve function and hearing presser vation显示文摘 | Ciricl Zhao jc Rosenblatt S et a1 | 2005 | J Neurosurgery2005，56，3： | 1 |
| 14 | A Study of threedismensional organi-zation of the human diaphragmatic lymphatic lacunae and lymphatic drainage units显示文摘 | Li JC Zhao ZR Zhou JL | 1996 | AnnAnat1996，178，： | 1 |
| 15 | Strain measurement by cardiovas- cular magnetic resonance in pediatric cancer survivors: validation of teature tracking against ha~rnonic phase imaging显示文摘 | Lu JC Connelly JA Zhao L | 2014 | Pediatr Radiol2014，44，： | 1 |
| 16 | Spinal cyelooxyge- nase-2 is involved in development of allodynia after nerve injury in rats显示文摘 | Zhao Z Chen SR Eisenaeh JC | 2000 | Neuroseience2000，97，4： | 1 |
| 17 | Enhancement of photocatalytic activity of mesoporous TiO2 by using carbon nanotubes显示文摘 | Yu Y Yu JC Yu JG Kwok YC Che YK Zhao JC | 2005 | Appl Catal A-Gen2005，289，2： | 1 |
| 18 | Spinal cyclooxygenase-2 is involved in development of allodynia after nerve injury in rats 显示文摘 | Zhao Z Chen SR Eisenach JC | 2000 | Neuroscience2000，97，4： | 1 |
| 19 | Functional analysis of long noncoding RNAs in development and disease显示文摘 | Chen LL Zhao JC | 2014 | Adv Exp Med Biol2014，825，： | 1 |
| 20 | Enhancement of adsorption and photocatalytic activity of TiO2 by using carbon nanotubes for the treatment of azo dye显示文摘 | Yu Y Yu JC Chan CY Che YK Zhao JC Ding L | 2005 | Appl Catal B-Environ2005，61，12： | 1 |
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