4篇
您的检索式：作者名="Zhao Zaixin"
|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Sequencing and analysis for the full-length genome RNA of foot-and-mouth disease virus China/99显示文摘The complete nucleotide sequence of genomic RNA of foot and mouth disease virus (FMDV) strain China/99 from infected bovine tongue epithelium is presented. The nucleotide sequence extending from the 5′ end of the genomic RNA to the 5′ end of poly (A) tail contains 8173 nucleotides (nt). Its open reading frame, which encodes a single polypeptide of 2332 amino acids, encompasses 6999 nt starting from the initiation codon AUG and terminating at the UAA codon 93 bases upstream from the 5′ end of poly (A) tract. The 5′ untranslated region (UTR) is composed of 1081nt. The consensus of the 1d gene of FMDV strain China/99 compared with that of UKG/6/2001, UKG/12/2001, China/99HN4 and China/3/Tibet is over 97%. The result showed the stains belong to the members of the Pan-Asia family. There is a remarkable differentiation in the function-unknown (FUR), p2 and p3 regions between FMDV isolates from infected cattle and swine, especially in 3a gene. No deletion was found in genes l, 1a, 1b, 2a, 2c, 3b, and 3d. These genes might be indispensable to the surviving of FMDV. The secondary structures of small (S) fragments, FUR and an internal ribosome entry site can be classified into three types, and the S fragment and 3′UTR of the positive-sense RNA fold into stem-loop structures similar to the shape of clover. | ZHANG Xiansheng LIU Zaixin ZHAO Qizu CHANG Huiyun XIE Qingge | 2004 | Science China(Life Sciences)2004，47，1： | 3 |
| 2 | Considering for the blanket structure scheme of HCCB DEMO显示文摘For the solid blanket concept of helium cooled ceramic breeder (HCCB) demonstration fusion power plant (DEMO), a feasible blanket structure with configuration 2×X is proposed as considering relatively low temperature limit of neutron multiplier beryllium pebbles. Based on that, preliminary design for the typical blanket module of HCCB DEMO has been carried out and verified by thermal-hydraulic analysis and structural analysis. Furthermore, the specific relationship of maximum temperature depended on the surface heating of blanket key part first wall (FW) is also analyzed. | Zhou Zhao Zaixin Li Xiaoyu Wang Xueren Wang Kaiming Feng | 2019 | Theoretical & Applied Mechanics Letters2019，9，3： | 2 |
| 3 | Cloning and Sequence Analysis of Mutant OmpF from Antibiotic Resistant Escherichia coli显示文摘[Objective]This study aimed to investigate the mutations of OmpF from an isolated antibiotic resistant Escherichia coli strain.[Methods]The mutant OmpF(mOmpF)from antibiotic resistant E.coli was amplified by PCR with Pfu DNA polymerase and ligated into the expression vector pET28a.Subsequently,the expression vector pET28-mOmpF was sequenced and analyzed by DNAMAN software and Swiss-Model online.[Result]Sequence analysis revealed that the open reading fragment of mOmpF was 903 bp long,which was mutated dramatically compared to that of the 1 020 bp long model OmpF.The DNA sequence shared only54.5%homology with OmpF.mOmpF was 44.6%identical to that of OmpF.Protein structure predication and analysis through Swiss-Model online suggested that the structure of mOmpF changed dramatically compared to OmpF.[Conclusion]The present study provided basis for further analyzing the relationships between the structure and functions of mOmpF from antibiotic resistant E.coli. | Xin NIE Li ZHANG Tingting LIU Yang ZHAO Zaixin LI Zhiping ZHAO | 2014 | Agricultural Biotechnology2014，3，3： | 1 |
| 4 | Neighbourhood weighted fuzzy c-means clustering algorithm for image segmentation显示文摘 | Zhao Zaixin Cheng Lizhi Cheng Guangquan | 2014 | Image Proeessing2014，8，3： | 1 |
您所在的机构暂未开通此部分的功能，请联系管理员