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| 1 | Improvement of wheat drought and salt tolerance by expression of a stress- inducible transcription factorGmDREB of soybean (Glycinemax)显示文摘Under stress conditions such as droughthigh-salinity and low-temperature, the transcription factorof DREB (dehydration responsive element binding proteins)improved efficiently stress resistance by regulating the ex-pression of its downstream genes with various environmentastress resistance in plants. GmDREB gene (GenBank Acces-sion No. AF514908) encoding a stress-inducible transcriptionfactor was cloned by screening a cDNA library of Glycinemax cv. Jinong 27 with yeast one-hybrid method. GmDREBgene was 910 bp in length and encoded 174 amino acids con-taining a conserved AP2/EREBP DNA-binding domain of 58amino acids. Two conserved functional amino acids, valineand glutamic acid, were located on the 14th and the 19thamino acid residues in the conserved structural domain. Analkaline amino acid region (KKR) related to a nuclear local-ization signal was at the N-terminal, while an acidic aminoacid region (DDD) related to trans-activation was at theC-terminal. Plant expression vectors were constructed andtransformed into wheat by bombardment. In total, 13 trans-genic plants with Ubi::GmDREB and 11 transgenic plantswith rd29A::GmDREB were identified from 103 regenerationplants by molecular analysis. The drought and salt tolerancesof T1 transgenic lines with Ubi::GmDREB orrd29A::GmDREB were demonstrated to be improved ascompared to wild type. The result also suggested that bothUbiquitin and rd29A promoters could effectively drive theexpression of the GmDREB gene and enhance drought andsalt tolerance of T1 plants. | GAO Shiqing XU Huijun CHENG Xianguo CHEN Ming XU Zhaoshi LI Liancheng YE Xingguo DU Lipu HAO Xiaoyan MA Youzhi | 2005 | Chinese Science Bulletin2005，50，23： | 30 |
| 2 | Expression of an(E)-β-farnesene synthase gene from Asian peppermint in tobacco affected aphid infestation显示文摘Aphids are major agricultural pests that cause significant yield losses in crop plants each year.(E)-β-farnesene(EβF) is the main or only component of an alarm pheromone involved in chemical communication within aphid species and particularly in the avoidance of predation. EβF also occurs in the essential oil of some plant species, and is catalyzed by EβF synthase. By using oligonucleotide primers designed from the known sequence of an EβF synthase gene from black peppermint(Mentha × piperita), two cDNA sequences, MaβFS1 and MaβFS2, were isolated from Asian peppermint(Mentha asiatica). Expression pattern analysis showed that the MaβFS1 gene exhibited higher expression in flowers than in roots, stems and leaves at the transcriptional level. Overexpression of MaβFS1 in tobacco plants resulted in emission of pure EβF ranging from 2.62 to 4.85 ng d-1g-1of fresh tissue. Tritrophic interactions involving peach aphids(Myzus persicae), and predatory lacewing(Chrysopa septempunctata) larvae demonstrated that transgenic tobacco expressing MaβFS1 had lower aphid infestation. This result suggested that the EβF synthase gene from Asian peppermint could be a good candidate for genetic engineering of agriculturally important crop plants. | Xiudao Yu Yongjun Zhang Youzhi Ma Zhaoshi Xu Genping Wang Lanqin Xia | 2013 | The Crop Journal2013，1，1： | 6 |
| 3 | Temporal and spatial variability of phytoplankton in Lake Poyang: The largest freshwater lake in China显示文摘 | Zhaoshi Wu Yongjiu Cai Xia Liu Cai Ping Xu Yuwei Chen Lu Zhang | 2013 | Journal of Great Lakes Research2013，，： | 3 |
| 4 | A Rare Population of CD24 + ITGB4 + Notch hi Cells Drives Tumor Propagation in NSCLC and Requires Notch3 for Self-Renewal显示文摘 | Yanyan Zheng Cecile C. de la Cruz Leanne C. Sayles Chris Alleyne-Chin Dedeepya Vaka Tim D. Knaak Marty Bigos Yue Xu Chuong D. Hoang Joseph B. Shrager Hans Joerg Fehling Dorothy French William Forrest Zhaoshi Jiang Richard A.D. Carano Kai H. Barck Erica L. | 2013 | Cancer Cell2013，，1： | 1 |
| 5 | (E)-β-Farnesene synthase genes affect aphid (Myzus persicae) infestation in tobacco (Nicotiana tabacum)显示文摘 | Xiudao Yu Huw Jones Youzhi Ma Genping Wang Zhaoshi Xu Baoming Zhang Yongjun Zhang Guangwei Ren John Pickett Lanqin Xia | 2012 | Functional & Integrative Genomics2012，，1： | 1 |
| 6 | Characterization of the TaAIDF a gene encoding a CRT/DRE - binding factor responsive to drought, high - salt, and cold stress in wheat 显示文摘 | Xu Zhaoshi Ni Zhiyong Liu Li | 2008 | Mol Genet Ge- nomics2008，280，6： | 1 |
| 7 | Isolation and functional characterization of HvDREB1 - a gene encoding a dehydration respon- sive element binding protein in Hordeum vulgare显示文摘 | Xu Zhaoshi Ni Zhiyong Li Zhiyong | 2009 | J Plant Res2009，122，1： | 1 |
| 8 | The soybean GmbZIP1 transcription factor enhances multiple abiotic stress tolerances in transgenic plant显示文摘 | Gao Shiqing Chen Ming Xu Zhaoshi | 2011 | Plant Molecular Biology2011，75，6： | 1 |
| 9 | Genomic analysis of isopentenyltransferase genes and functional characterization of TaIPT8 indicates positive effects of cytokinins on drought tolerance in wheat显示文摘Cytokinins(CKs)function in plant development and during stress responses,but their role in drought tolerance in wheat is unknown.In the present study,24 isopentenyltransferase(IPT)genes,encoding ratelimiting enzymes in CK biosynthesis were identified in the wheat genome.The chromosomal locations and structures of the genes,protein properties,and phylogenetic relationships were characterized.ATP/ADP TaIPT genes showed tissue-specific expression.TaIPT2,TaIPT7,and TaIPT8 expression was rapidly induced by 0.5–1 h drought treatments,which decreased to low levels after 2 h drought treatment,as did most other TaIPT genes.TaIPT8-5a/5b/5d triple mutants showed decreased levels of tZtype CK under normal and drought conditions and reduced drought tolerance,which,however,did not manifest as phenotype alterations.By contrast,transgenic wheat plants with drought-induced TaIPT8showed increased drought tolerance.Our study provides a foundation for further investigation of TaIPT genes and novel insights into the role of CKs in the drought response of wheat. | Na Wang Jun Chen Yuan Gao Yongbin Zhou Ming Chen Zhaoshi Xu Zhengwu Fang Youzhi Ma | 2023 | The Crop Journal2023，11，1： | 0 |
| 10 | Genome-wide association study of seedling nitrogen-use efficiency-associated traits in common wheat(Triticum aestivum L.)显示文摘Nitrogen(N)fertilizer application is essential for crop-plant growth and development.Identifying genetic loci associated with N-use efficiency(NUE)could increase wheat yields and reduce environmental pollution caused by overfertilization.We subjected a panel of 389 wheat accessions to N and chlorate(a nitrate analog)treatments to identify quantitative trait loci(QTL)controlling NUE-associated traits at the wheat seedling stage.Genotyping the panel with a 660K single-nucleotide polymorphism(SNP)array,we identified 397 SNPs associated with N-sensitivity index and chlorate inhibition rate.These SNPs were merged into 49 QTL,of which eight were multi-environment stable QTL and 27 were located near previously reported QTL.A set of 135 candidate genes near the 49 QTL included TaBOX(F-box family protein)and TaERF(ethylene-responsive transcription factor).A Tabox mutant was more sensitive to low-N stress than the wild-type plant.We developed two functional markers for Hap 1,the favorable allele of TaBOX. | Huawei Shi Weichong Wang Lifeng Gao Jirong Wu Chengmei Hu Huishu Yan Yugang Shi Ning Li Youzhi Ma Yongbin Zhou Zhaoshi Xu Jun Chen Wensi Tang Kai Chen Daizhen Sun Yuxiang Wu Ming Chen | 2024 | The Crop Journal2024，12，1： | 0 |
| 11 | Case report of antiseizure medicine-induced long QT syndrome and a literature review显示文摘Background To realize the clinical characteristics of long QT syndrome(LQTS)caused by antiseizure medicines(ASMs),and to improve the prevention and management of ASM-acquired QT syndrome.Case presentation A case of ASM-acquired QT syndrome was diagnosed and relevant literature was reviewed.The case was a 7-year-old boy who presented with a sudden onset of panic followed by changes in consciousness,with or without convulsions,lasting from tens of seconds to 3 min.The patient then received antiepileptic treatment with valproic acid,levetiracetam and oxcarbazepine and was seizure free for about a year.However,on August 12,2021,his illness flared up again.Electroencephalogram(EEG)showed the background activity was slow,and no obvious epileptic discharge was detected.But electrocardiogram(ECG)showed a surprisingly prolonged QT interval(770 ms).Torsades de Pointes was found during Holter monitoring,while electrolyte levels were normal.The ECG recordings gradually returned to normal after stopping ASMs.For literature search,only 21 related papers were obtained after reading titles and full-texts of 105 English-language papers retrieved using keywords'acquired QT interstitial syndrome/acquired Long QT Syndrome(aLQTS)'and'anti-epileptic seizure drugs/ASMs',in the databases of Wanfang,CNKI,Pubmed,and other databases,from publication year 1965 to October 26,2021.There are 12 types of drug-acquired LQTS caused by ASMs,most of which are Na+blockers,but LQTS caused by oxcarbazepine had not been reported previously.Conclusions ASMs such as oxcarbazepine can cause acquired LQTS.When Na+or K+channel blockers are used clinically,ECG should be reviewed regularly and abnormal ECG should be intervened in time to reduce iatrogenic accidents in patients with epilepsy. | Xiongying Yu Jian Zha Zhaoshi Yi Xiaoyan Li Hui Chen Yuxin Xu Jianmin Zhong | 2022 | Acta Epileptologica2022，5，4： | 0 |
| 12 | TaNAC48 positively regulates drought tolerance and ABA responses in wheat(Triticum aestivum L.)显示文摘NAC family transcription factors(TFs) are important regulators in plant development and stress responses. However, the biological functions of NAC TFs in wheat are rarely studied. In this study, 43 putative drought-induced NAC genes were identified from de novo transcriptome sequencing data of wheat following drought treatment. Twelve wheat NACs along with ten known stress-related NACs from Arabidopsis and rice were clustered into Group II based on a phylogenetic analysis. Ta NAC48, which showed a higher and constitutive expression level in Group Ⅱ, was selected for further investigation.Ta NAC48 transcript was up-regulated by drought, PEG, H_(2)O_(2) and abscisic acid(ABA) treatment and encoded a nuclear localized protein. Overexpression of Ta NAC48 significantly promoted drought tolerance with increased proline content, and decreased rates of water loss, malondialdehyde(MDA), H_(2)O_(2) and O_(2)^(-) content. Root length and a stomatal aperture assay confirmed that Ta NAC48-overexpression plants increased sensitivity to ABA. Electrophoretic mobility shift assay(EMSA) and luciferase reporter analysis indicated that Ta AREB3 could bind to a cis-acting ABA-responsive element(ABRE) on Ta NAC48 promoter and activate the expression of Ta NAC48. These results suggest that Ta NAC48 is essential in mediating crosstalk between the ABA signaling pathway and drought stress responses in wheat. | Jun Chen Yan Gong Yuan Gao Yongbin Zhou Ming Chen Zhaoshi Xu Changhong Guo Youzhi Ma | 2021 | The Crop Journal2021，9，4： | 0 |
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