|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Correction of a genetic disease by CRISPR-Cas9-mediated gene editing in mouse spermatogonial stem cells显示文摘Spermatogonial 干细胞(SSC ) 能在移植以后生产众多的男配偶子进接受者睾丸,介绍为基因治疗和修改基因的动物的连续生产的一条珍贵途径。然而, SSC 的成功的基因操作被限制了,部分由于复杂性和当前可得到的基因编辑技术的低效率。这里,我们证明有效基因修正能用 CRISPR-Cas9 系统被介绍进 SSC。我们使用了 CRISPR-Cas9 系统变异在 SCC 的 EGFP transgene 或内长的 Crygc 基因。变异的 SSC 在移植以后经历了精子发生进不肥沃的老鼠睾丸的生精的小管。圆 spermatids 被产生并且在进成熟卵母细胞的注射以后,支持了显示相应变异的显型的异质接合的后代的生产。而且,在 Crygc 的一个引起疾病的变化(在 SSC 先存在的 Crygc −/−) 能乐意地到加入的 CRISPR-Cas9-induced nonhomologous 结束(NHEJ ) 或指导相同的修理(HDR ) 被修理,导致没有是的离开目标修正的证据带改正的基因的 SSC 线由整个染色体的定序出现。用从这些线产生的圆 spermatids 的授精在 100% 的效率与改正的显型产生了后代。我们的结果表明有效基因由 CRISPR-Cas9 系统在老鼠 SSC 编辑,并且提供在 SSC 经由基因修正治好基因疾病的原则的证明。 | Yuxuan Wu Hai Zhou Xiaoying Fan Ying Zhang Man Zhang Yinghua Wang Zhenfei Xie Meizhu Bai Qi Yin Dan Liang Wei Tang Jiaoyang Liao Chikai Zhou Wujuan Liu Ping Zhu Hongshan Guo Hong Pan Chunlian Wu Huijuan Shi Ligang Wu Fuchou Tang Jinsong Li | 2015 | Cell Research2015，25，1： | 37 |
| 2 | Dosage effect of multiple genes accounts for multisystem disorder of myotonic dystrophy type 1显示文摘Multisystem manifestations in myotonic dystrophy type 1(DM1)may be due to dosage reduction in multiple genes induced by aberrant expansion of CTG repeats in DMPK,including DMPK,its neighboring genes(SIX5 or DMWD)and downstream MBNLh However,direct evidence is lacking.Here,we develop a new strategy to generate mice carrying multigene heterozygous mutations to mimic dosage reduction in one step by injection of haploid embryonic stem cells with mutant Dmpk,Six5 and Mbnll into oocytes.The triple heterozygous mutant mice exhibit adult-onset DM1 phenotypes.With the additional mutation in Dmwd,the quadruple heterozygous mutant mice recapitulate many major manifestations in congenital DM1.Moreover,muscle stem cells in both models display reduced sternness,providing a unique model for screening small molecules for treatment of DM1.Our results suggest that the complex symptoms of DM1 result from the reduced dosage of multiple genes. | Qi Yin Hongye Wang Na Li Yifu Ding Zhenfei Xie Lifang Jin Yan Li Qiong Wang Xinyi Liu Liuqing Xu Qing Li Yongjian Ma Yanbo Cheng Kai Wang Cuiqing Zhong Qian Yu Wei Tang Wanjin Chen Wenjun Yang Fan Zhang Chen Ding Lan Bao Bin Zhou Ping Hu Jinsong Li | 2020 | Cell Research2020，30，2： | 7 |
| 3 | An Efficient Method for Estimating Capability Curve of Virtual Power Plant显示文摘To incorporate the operating constraints of a virtual power plant(VPP)in transmission-level operation and market clearing,the concept of the VPP capability curve(VPP-CC)is proposed which explicitly characterizes the allowable range of active and reactive power outputs of a VPP.A two-step projection-based calculation framework is proposed to approximate the VPP-CC by the convex hull of critical points on its perimeter.The output of the proposed algorithm is concise and can be easily incorporated in the existing system operation and market clearing.Case studies based on the IEEE 33 and 123 test feeders show the computational efficiency of the proposed method outperforms existing methods by 4~7 times.Additionally,many fewer inequalities are needed to depict the VPP-CC while achieving the comparative approximation accuracy compared to sampling-based methods,which will relieve the communication and computation burden. | Zhenfei Tan Haiwang Zhong Xuanyuan Wang Honghai Tang | 2022 | CSEE Journal of Power and Energy Systems2022，8，3： | 5 |
| 4 | A new 5G radio evolution towards 5G-Advanced显示文摘The evolution of the fifth-generation(5G)new radio(NR)has progressed swiftly since the third generation partnership project(3GPP)standardized the first NR version(Release 15)in mid-2018.Nowadays,the world’s leading carriers are competing to provide various commercial services over 5G networks.Looking ahead to 2025 and beyond,it is expected that over 6.5 million 5G base stations will be installed to offer services to over 58%of the world’s population via over 100 billion 5G connections.Following the rapid development of 5G,an increasing number of commercialization use cases will drive the 5G network to continuously improve performance and expand capabilities.Hence,it is the right time to consider a welldefined framework and standardization for 5G NR evolution(5G-Advanced)to support commercialization between 2025 and 2030.First,this study addresses the key driving forces,requirements,usage scenarios,and capabilities of 5G-Advanced;then,it highlights the main technological challenges and introduces the top 10 promising technological directions in detail.Finally,other fascinating technological directions in5G-Advanced are shortly mentioned. | Jiyong PANG Shaobo WANG Zhenfei TANG Yanmin QIN Xiaofeng TAO Xiaohu YOU Jinkang ZHU | 2022 | Science China(Information Sciences)2022，65，9： | 1 |
| 5 | Effects of substitution for carbon black with graphene oxide or graphene on the morphology and performance of natural rubber/carbon black composites显示文摘 | Ganwei Yang Zhenfei Liao Zhijun Yang Zhenghai Tang Baochun Guo | 2015 | J. Appl. Polym. Sci2015，，15： | 1 |
| 6 | Similarities and Differences of CT Features between COVID-19 Pneumonia and Heart Failure显示文摘Aims:During the COVID-19 epidemic,chest computed tomography(CT)has been highly recommended for screen-ing of patients with suspected COVID-19 because of an unclear contact history,overlapping clinical features,and an overwhelmed health system.However,there has not been a full comparison of CT for diagnosis of heart failure or COVID-19 pneumonia.Methods:Patients with heart failure(n=23)or COVID-19 pneumonia(n=23)and one patient with both diseases were retrospectively enrolled.Clinical information and chest CT images were obtained and analyzed.Results:There was no difference in ground-glass opacity,consolidation,crazy paving pattern,the lobes affected,and septal thickening between heart failure and COVID-19 pneumonia.However,a less rounded morphology(4%vs.70%,P=0.00092),more peribronchovascular thickening(70%vs.35%,P=0.018)and fi ssural thickening(43%vs.4%,P=0.002),and less peripheral distribution(30%vs.87%,P=0.00085)were found in the heart failure group than in the COVID-19 group.Importantly,there were also more patients with upper pulmonary vein enlargement(61%vs.4%,P=0.00087),subpleural effusion(50%vs.0%,P=0.00058),and cardiac enlargement(61%vs.4%,P=0.00075)in the heart failure group than in the COVID-19 group.Besides,more fi brous lesions were found in the COVID-19 group,although there was no statistical difference(22%vs.4%,P=0.080).Conclusions:Although there is some overlap of CT features between heart failure and COVID-19,CT is still a useful tool for differentiating COVID-19 pneumonia. | Zhaowei Zhu Jianjun Tang Xiangping Chai Zhenfei Fang Qiming Liu Xinqun Hu Danyan Xu Jia He Liang Tang Shi Tai Yuzhi Wu Shenghua Zhou | 2021 | Cardiovascular Innovations and Applications2021，，3： | 0 |
| 7 | In-Hospital Cardiac Arrest after Emotional Stress in a Patient Hospitalized with Gastrointestinal Symptoms and Chronic Anxiety Disorder显示文摘Background:We report an acute emotional stress-induced in-hospital cardiac arrest in a patient admitted with gas-trointestinal symptoms after experiencing chronic anxiety disorder.Case Presentation:The patient was admitted to the Second Xiangya Hospital,Central South University,with gastrointestinal symptoms and chronic anxiety disorder,and experienced cardiac arrest during hospitalization after acute emotional stress.Malignant ventricular tachycardia and cardiogenic shock were evidenced in this patient after the acute emotional stress.Severe and extensive coronary spasm was confi rmed by emergency coronary angiography,and coronary spasm was relieved by intracoronary injection of nitroglycerin.The patient recovered from myocardial infarction with nonobstructive coronary arteries.However,the patient developed acute kidney dysfunction and severe pulmonary infection and eventually died of respiratory circulatory failure on the ninth day after the successful rescue.Conclusions:Acute emotional stress on top of chronic anxiety disorder in patients hospitalized for noncardiovascu-lar reasons might lead to the development of life-threatening cardiovascular diseases,including coronary artery spasm and myocardial infarction with nonobstructive coronary arteries.Psychological management is of importance to im-prove the outcome of these patients. | Tao Tu Jiang Li Zhenfei Fang Xinqun Hu Jianjun Tang Yanshu Zhao Liang Tang Shenghua Zhou | 2021 | Cardiovascular Innovations and Applications2021，，3： | 0 |
您所在的机构暂未开通此部分的功能，请联系管理员