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| 1 | Integrated transcriptome and proteome analysis provides insight into chilling-induced dormancy breaking in Chimonanthus praecox显示文摘Chilling has a critical role in the growth and development of perennial plants.The chilling requirement(CR)for dormancy breaking largely depends on the species.However,global warming is expected to negatively affect chilling accumulation and dormancy release in a wide range of perennial plants.Here,we used Chimonanthus praecox as a model to investigate the CR for dormancy breaking under natural and artificial conditions.We determined the minimum CR(570 chill units,CU)needed for chilling-induced dormancy breaking and analyzed the transcriptomes and proteomes of flowering and non-flowering flower buds(FBs,anther and ovary differentiation completed)with different CRs.The concentrations of ABA and GA3 in the FBs were also determined using HPLC.The results indicate that chilling induced an upregulation of ABA levels and significant downregulation of SHORT VEGETATIVE PHASE(SVP)and FLOWERING LOCUS T(FT)homologs at the transcript level in FBs when the accumulated CR reached 570 CU(IB570)compared to FBs in November(FB.Nov,CK)and nF16(non-flowering FBs after treatment at 16℃for−300 CU),which suggested that dormancy breaking of FBs could be regulated by the ABA-mediated SVP-FT module.Overexpression in Arabidopsis was used to confirm the function of candidate genes,and early flowering was induced in 35S::CpFT1 transgenic lines.Our data provide insight into the minimum CR(570 CU)needed for chilling-induced dormancy breaking and its underlying regulatory mechanism in C.praecox,which provides a new tool for the artificial regulation of flowering time and a rich gene resource for controlling chilling-induced blooming. | Zhineng Li Ning Liu Wei Zhang Chunyu Wu Yingjie Jiang Jing Ma Mingyang Li Shunzhao Sui | 2020 | Horticulture Research2020，7，1： | 6 |
| 2 | Investigation of the hydrodynamics of slug flow in airlift pumps显示文摘A slug flow model considering the dispersed bubbles entrained from the tail of Taylor bubble(TB) and recoalesced with the successive TB was proposed. Experiment was conducted to test the validity of this model by using a high-speed camcorder and particle image velocimetry(PIV). It was found that the model was valid for predicting the characteristics of slug flow in airlift pump within average error of 14%. Moreover, large pipe diameter was found to accelerate the rise velocity of TB and decreases void fraction in liquid slug by a small margin. | Zhineng Wang Yong Kang Xiaochuan Wang Shijing Wu Xiaoyong Li | 2018 | Chinese Journal of Chemical Engineering2018，26，12： | 3 |
| 3 | PEGylated BODIPY assembling fluorescent nanoparticles for photodynamic therapy显示文摘Two amphiphilic macromolecules were synthesized from polyethylene glycol monomethylether(PEG)and borondipyrrolmethene(BODIPY) via one-pot multicomponent Passerini reaction,and they could self-assemble into stable nanoparticles(NPs) in aqueous media.The optical properties,including fluorescence resonance energy transfer(FRET) were studied in detail.The obtained NPs possess good cytocompatibility,and could be used for living cell imaging and effective photodynamic therapy(PDT).These results shed light on one-pot synthesis of PEGylated fluorescent nanoparticles via multicomponent reaction for biomedical application. | Yu Zhu Wenhai Lin Wei Zhang Yuqin Feng Zhineng Wu Li Chen Zhigang Xie | 2017 | Chinese Chemical Letters2017，28，9： | 1 |
| 4 | Intron-retained alternatively spliced VIN3-LIKE 2 gene from Chimonanthus praecox promotes flowering in transgenic Arabidopsis显示文摘Vernalization is a process of acquiring or accelerating the flowering ability by prolonged cold exposure.VERNALIZATION INSENSITIVE3(VIN3)is induced by chilling and is extremely important for the vernalization response in Arabidopsis thaliana.However,the issue of the induction of the VIN3-LIKE genes in wintersweet(Chimonanthus praecox)has been largely neglected.In the present study,we explored the molecular regulation of the PHD type finger protein-encoding gene CpVIL2 in relation to the growth and development of wintersweet in Arabidopsis.In wintersweet,quantitative real-time PCR(qRT-PCR)analysis showed that the relative expression of CpVIL2-As2i(intron-retained alternatively spliced in the second intron)was extremely higher in the pistils than in the other tissues.And the relative CpVIL2-As2i expression in flower buds(FBs)treated at 8°C was higher than that of FBs in December,2016 under natural conditions,which was not detected in non-flowering FBs at 16°C.In Arabidopsis,the expression patterns of the CpVIL2-As2i gene were detected at first in CpVIL2-As2i pro::GUS(β-glucuronidase)lines,with predominantly higher expression in flowers and inflorescence.Meanwhile,the hormone-induced expression profiles of the CpVIL2-As2i promoter were confirmed using exogenous induction by abscisic acid(ABA)and indole acetic acid(IAA)phytohormones,where the GUS enzyme activity obviously decreased compared with that of control.In comparison with Arabidopsis/Col-0,early flowering was detected in ectopic 35S::CpVIL2-As2i lines.Overall,these results demonstrated the function of the CpVIL2-As2i gene,at the same time,provided us with new insights into the molecular mechanisms of early flowering and complex regulatory networks of vernalization in wintersweet. | Tian Xu Ning Liu Chunyu Wu Yi Zhang Mingkang Tian Shunzhao Sui Zhineng Li | 2023 | Horticultural Plant Journal2023，9，4： | 0 |
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