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| 1 | In tegrative iTRAQ-based proteomic and transcriptomic analysis reveals the accumulation patter ns of key metabolites associated with oil quality during seed ripening of Camellia oleifera显示文摘Camellia oleifera(C.oleifera)is one of the four major woody oil-bearing crops in the world and has relatively high ecological,economic,and medicinal value.Its seeds undergo a series of complex physiological and biochemical changes during ripening,which is mainly manifested as the accumulation and transformation of certain metabolites closely related to oil quality,especially flavonoids and fatty acids.To obtain new insights into the underlying molecular mechanisms,a parallel analysis of the transcriptome and proteome profiles of C.oleifera seeds at different maturity levels was conducted using RNA sequencing(RNA-seq)and isobaric tags for relative and absolute quantification(iTRAQ)complemented with gas chromatography-mass spectrometry(GC-MS)data.A total of 16,530 transcripts and 1228 proteins were recognized with significant differential abundances in pairwise comparisons of samples at various developmental stages.Among these,317 were coexpressed with a poor correlation,and most were involved in metabolic processes,including fatty acid metabolism,a-linolenic acid metabolism,and glutathione metabolism.In addition,the content of total flavonoids decreased gradually with seed maturity,and the levels of fatty acids generally peaked at the fat accumulation stage;these results basically agreed with the regulation patterns of genes or proteins in the corresponding pathways.The expression levels of proteins annotated as upstream candidates of phenylalanine ammonia-lyase(PAL)and chalcone synthase(CHS)as well as their cognate transcripts were positively correlated with the variation in the flavonoid content,while shikimate O-hydroxycinnamoyltransferase(HCT)-(encoding genes had the opposite pattern.The increase in the abundance of proteins and mRNAs corresponding to alcohol dehydrogenase(ADH)was associated with a reduction in linoleic acid synthesis.Using weighted gene coexpression network analysis(WGCNA),we further identi fied six unique modules related to flavonoid,oil,and fatty acid anabolism that contained hub genes or proteins similar to transcription factors(TFs),such as MADS intervening keratin-like and C-terminal(MIKC_MAD s),type-B authentic response regulator(ARR-B),and basic helix-loop-helix(bH〔H).Finally,based on the known metabolic pathways and WGCNA combined with the correlation analysis,fi ve coexpressed transcripts and proteins composed of cinnamyl-alcohol dehyd rogenases(CADs),caffeicacid 3-O-methyltransferase(COMT),fl avonol synthase(FLS),and 4-coumarate:CoA ligaas(4CL)were screened out.With this exploratory multiomics dataset,our results presented a dynamic picture regarding the maturation process of C.oleifera seeds on Hainan Island,not only revealing the temporal specific expression of key candidate genes and proteins but also providing a scientific basis for the genetic improvement of this tree species. | Zhouchen Ye Jing Yu Wuping Yan Junfeng Zhang Dongmei Yang Guanglong Yao Zijin Liu Yougen Wu Xilin Hou | 2021 | Horticulture Research2021，8，1： | 3 |
| 2 | Propagation characteristics of guided waves in a liquid filled pipe embedded in a porous medium显示文摘A model of a liquid-filled pipe embedded in a porous medium is built to research its wave propagation characteristics,and to analyze the effect of the parameters of porous media on the dispersion.The dispersion equations are established on the basis of the elastic-dynamic theory of the liquid-saturated porous solid.The characteristic of dispersion and the time domain waveform in pipes of different thicknesses and with different porous-medium parameters are discussed theoretically and numerically.Results reveal that the porosity has little impact on dispersion,and the attenuation of guided wave increases with porosity,whilst the porosity influences the displacement amplitude of the time domain waveform.It is hard to detect the permeability variation of the media by the dispersion.The drawn conclusion can provide some theoretical instruction and guidance for the nondestructive testing of buried pipe. | JIANG Jian HAN Qingbang XU Zhouchen JIA Jing LIANG Dong ZHONG Xiaomin | 2020 | Chinese Journal of Acoustics2020，39，1： | 2 |
| 3 | Limits of learning-based super-resolution algorithms显示文摘 | LIN Zhouchen HE Junfeng TANG Xiaoou | 2008 | International Journal of Computer Vision(S0920-5691)2008，80，1： | 1 |
| 4 | Ro- bust recovery of subspace structures by low-rank represen- tation显示文摘 | Liu Guangcan Lin Zhouchen Yan Shuicheng | 2013 | IEEE Transactions on Pattern Analysis and Ma- chine Intelligence2013，35，1： | 1 |
| 5 | Robust recovery ofsubspace structures by low-rank representation 显示文摘 | Liu Guangcan Lin Zhouchen Yan Shuicheng’ | 2013 | IEEE Trans onPattern Analysis and Machine Intelligence2013，35，1： | 1 |
| 6 | Fast, automatic and fine-grained tampered JPEG image detection via DCT co- efficient analysis显示文摘 | LIN Zhouchen HE Junfeng TANG Xiaoou | 2009 | Pattern Recognition2009，42，11： | 1 |
| 7 | Effect of Sb on the Microstructure and Mechanical Properties of AZ91 Magnesium Alloy 显示文摘 | QudongWang Wen zhouChen WenjiangDing | 2001 | Metallurgical and Materials Transactions A2001，32，： | 1 |
| 8 | Robust recovery of subspace structures by low-rank representation显示文摘 | LIU Guangcan LIN Zhouchen YAN Shuicheng | | 0，，01： | 1 |
| 9 | Fast,automatic and fine-grained tampered JPEG image detection via DCT coefficient anal-ysis显示文摘 | Lin Zhouchen He Junfeng | 2009 | Pattern Recognition2009，42，11： | 1 |
| 10 | A geometric method for optimal design of color filter arrays显示文摘 | HAO Pengwei LI Yan LIN Zhouchen | 2011 | IEEE Trans Image Process2011，20，3： | 1 |
| 11 | Robust recovery of subspace structures by low-rank representation显示文摘 | Liu Guangcan Lin Zhouchen Yan Shuicheng | 2013 | IEEE Transactions on Pattern Analysis and Machine Intelligence2013，35，1： | 1 |
| 12 | Ll-norm kernel discriminant analysis via Bayes error bound optimizatin for robust feature extraction 显示文摘 | HENG Wenming LIN Zhouchen WANG Haixian | 2014 | IEEE Transactions on Neural Networks and Learning Systems(S 1045-9227)2014，25，4： | 1 |
| 13 | Fundamental Limits of Reconstruction-Based Super-Resolution Alorithms Under Local Translation显示文摘 | Lin Zhouchen Shum Heung-Yeung | 2004 | IEEE Trans on Pattern Analysis and Machine Intelligence2004，26，1： | 1 |
| 14 | Robust recovery of subspace structures by low-rank representation显示文摘 | Liu Guangcan Lin Zhouchen Yan Shuicheng | 2013 | IEEE Trans on PAMI2013，35，1： | 1 |
| 15 | The aug- mented lagrange multiplier method for exact recovery of cor-rupted low-rank matrices 显示文摘 | Lin Zhouchen Chen Minming Wu Leqiu | 2010 | Mathematics : Optimization and Control2010，26，1： | 1 |
| 16 | Robust reco- very of subspace structures by low-rank representation 显示文摘 | Liu Guangcan Lin Zhouchen ~an Shuicheng | 2013 | IEEE Trans on Pattern Analysis and Machine Intelligence2013，，： | 1 |
| 17 | Multi-output Regression on the Output Manifold显示文摘 | Liu Guangcan Lin Zhouchen Yu Yong | 2009 | Pattern Recog- nition2009，42，： | 1 |
| 18 | Fast, automatic and fine-grained tampered JPEG image detection via DCT coefficient analysis显示文摘 | Zhouchen Lin Junfeng He Xiaoou Tang Chi-Keung Tang | 2009 | Pattern Recognition2009，，11： | 1 |
| 19 | Fast convex optimization algorithms for exact recovery of a corrupted low-rank matrix显示文摘 | LIN Zhouchen GANESH A WRIGHT J | 2009 | International Workshop on Computational Advances in Multi-Sensor Adaptive Processing(CAMSAP)2009，61，： | 1 |
| 20 | Robust recovery of subspace structures by low-rank representation显示文摘 | Liu Guangcan Lin Zhouchen Yan Shuicheng | 2012 | IEEE Transactions on Software Engineering2012，35，1： | 1 |
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