4篇
您的检索式：作者名="Zhuoning Li"
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| 1 | Kinetics and mechanisms of mitotic inheritance of DNA methylation and their roles in aging-associated methylome deterioration显示文摘Mitotic inheritance of the DNA methylome is a challenging task for the maintenance of cell identity.Whether DNA methylation pattern in different genomic contexts can all be faithfully maintained is an open question.A replication-coupled DNA methylation maintenance model was proposed decades ago,but some observations suggest that a replication-uncoupled maintenance mechanism exists.However,the capacity and the underlying molecular events of replication-uncoupled maintenance are unclear.By measuring maintenance kinetics at the single-molecule level and assessing mutant cells with perturbation of various mechanisms,we found that the kinetics of replication-coupled maintenance are governed by the UHRF1–Ligase 1 and PCNA–DNMT1 interactions,whereas nucleosome occupancy and the interaction between UHRF1 and methylated H3K9 specifically regulate replication-uncoupled maintenance.Surprisingly,replication-uncoupled maintenance is sufficiently robust to largely restore the methylome when replication-coupled maintenance is severely impaired.However,solo-WCGW sites and other CpG sites displaying aging-and cancer-associated hypomethylation exhibit low maintenance efficiency,suggesting that although quite robust,mitotic inheritance of methylation is imperfect and that this imperfection may contribute to selective hypomethylation during aging and tumorigenesis. | Xuan Ming Zhuqiang Zhang Zhuoning Zou Cong Lv Qiang Dong Qixiang He Yangyang Yi Yingfeng Li Hailin Wang Bing Zhu | 2020 | Cell Research2020，30，11： | 4 |
| 2 | Author Correction: Kinetics and mechanisms of mitotic inheritance of DNA methylation and their roles in aging-associated methylome deterioration显示文摘We apologize for the mistake of the fund information in the acknowledgements.The correct information is as follows.This correction does not affect the conclusion of the work. | Xuan Ming Zhuqiang Zhang Zhuoning Zou Cong Lv Qiang Dong Qixiang He Yangyang Yi Yingfeng Li Hailin Wang Bing Zhu | 2021 | Cell Research2021，31，3： | 2 |
| 3 | Construction of immobilized films photocatalysts with CdS clusters decorated by metal Cd and BiOCl for photocatalytic degradation of tetracycline antibiotics显示文摘A kind of CdS/Cd-BiOCl immobilized films photocatalyst was prepared.The optical and physicochemical properties of the CdS/Cd-BiOCl photocatalysts were analysed,and the detailed characterization revealed CdS/Cd-BiOCl films photocatalyst with good charge carrier separation effect.The reusabilities and photocatalytic properties of the samples were studied.The 15%Cd S/Cd-Bi OCl photocatalyst exhibited superior performance in photocatalytic degradation of tetracycline(TC)and favorable stability under visible light irradiation.As for the photodegradation rate of TC,15%CdS/Cd-BiOCl exhibited an excellent photodegradation activity,which is 4.06 and 9.53 times higher than that of Cd S/Cd and Bi OCl,respectively.The results showed that dominant active species are·O_(2)^(-)and·OH radicals during photodegradation.The charge transfer in Z-scheme CdS/Cd-BiOCl films photocatalyst could synchronously generate conduct band(CB)electrons in BiOCl and valence band(VB)holes in CdS,and metal Cd served as electron mediator.This work can be a reference for the design of film photocatalysts and new insight for photodegradating towards contaminants. | Jiajia Li Ziwei Zhao Zhuoning Li Huijuan Yang Shijun Yue Yuping Tang Qizhao Wang | 2022 | Chinese Chemical Letters2022，33，8： | 0 |
| 4 | A genetically engineered Escherichia coli that senses and degrades tetracycline antibiotic residue显示文摘Due to the abuse of antibiotics,antibiotic residues can be detected in both natural environment and various industrial products,posing threat to the environment and human health.Here we describe the design and implementation of an engineered Escherichia coli capable of degrading tetracycline(Tc)-one of the commonly used antibiotics once on humans and now on poultry,cattle and fisheries.A Tcdegrading enzyme,TetX,from the obligate anaerobe Bacteroides fragilis was cloned and recombinantly expressed in E.coli and fully characterized,including its Km and kcat value.We quantitatively evaluated its activity both in vitro and in vivo by UVeVis spectrometer and LC-MS.Moreover,we used a tetracycline inducible amplification circuit including T7 RNA polymerase and its specific promoter PT7 to enhance the expression level of TetX,and studied the dose-response of TetX under different inducer concentrations.Since the deployment of genetically modified organisms(GMOs)outside laboratory brings about safety concerns,it is necessary to explore the possibility of integrating a kill-switch.Toxin-Antitoxin(TA)systems were used to construct a mutually dependent host-plasmid platform and biocontainment systems in various academic and industrious situations.We selected nine TA systems from various bacteria strains and measured the toxicity of toxins(T)and the detoxifying activity of cognate antitoxins(A)to validate their potential to be used to build a kill-switch.These results prove the possibility of using engineered microorganisms to tackle antibiotic residues in environment efficiently and safely. | Zepeng Mu Zhuoning Zou Ye Yang Wenbo Wang Yue Xu Jianyi Huang Ruiling Cai Ye Liu Yajin Mo Boyi Wang Yiqun Dang Yongming Li Yushan Liu Yueren Jiang Qingyang Tan Xiaohong Liu Cheng Hu Hua Li Sha Wei Chunbo Lou Yang Yu Jiangyun Wang | 2018 | Synthetic and Systems Biotechnology2018，3，3： | 0 |
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