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| 1 | Counteraction of ABA-Mediated Inhibition of Seed Germination and Seedling Establishment by ABA Signaling Terminator in Arabidopsis显示文摘Seed germination and seedling establishment are important for the reproductive success of plants,but seeds and seedlings typically encounter constantly changing environmental conditions.By inhibiting seed germination and post-germinative growth through the PYR1/PYL/RCAR ABA receptors and PP2C co-receptors,the phytohormone abscisic acid(ABA)prevents premature germination and seedling growth under unfavorable conditions.However,little is known about how the ABA-mediated inhibition of seed germination and seedling establishment is thwarted.Here,we report that ABA Signaling Terminator(ABT),a WD40 protein,efficiently switches off ABA signaling and is critical for seed germination and seedling establishment.ABT is induced by ABA in a PYR1/PYL/RCAR-PP2C-dependent manner.Overexpression of ABT promotes seed germination and seedling greening in the presence of ABA,whereas knockout of ABT has the opposite effect.We found that ABT interacts with the PYR1/PYL/RCAR and PP2C proteins,interferes with the interaction between PYR1/PYL4 and ABI1/ABI2,and hampers the inhibition of ABI1/ABI2 by ABA-bound PYR1/PYL4,thereby terminating ABA signaling.Taken together,our results reveal a core mechanism of ABA signaling termination that is critical for seed germination and seedling establishment in Arabidopsis. | Zhijuan Wang Ziyin Ren Chunhong Cheng Tao Wang Hongtao Ji Yang Zhao Zhiping Deng Liya Zhi Jingjing Lu Xinying Wu Shimin Xu Mengmeng Cao Hongtao Zhao Liu Liu Jiankang Zhu Xia Li | 2020 | Molecular Plant2020，13，9： | 10 |
| 2 | NUCLEAR PORE ANCHOR and EARLY IN SHORT DAYS 4 negatively regulate abscisic acid signaling by inhibiting Snf1-related protein kinase2 activity and stability in Arabidopsis显示文摘Abscisic acid(ABA)is a key regulator of plant responses to abiotic stresses,such as drought.Abscisic acid receptors and coreceptors perceive ABA to activate Snf1-related protein kinase2 s(SnRK2s)that phosphorylate downstream effectors,thereby activating ABA signaling and the stress response.As stress responses come with fitness penalties for plants,it is crucial to tightly control SnRK2 kinase activity to restrict ABA signaling.However,how SnRK2 kinases are inactivated remains elusive.Here,we show that NUCLEAR PORE ANCHOR(NUA),a nuclear pore complex(NPC)component,negatively regulates ABA-mediated inhibition of seed germination and post-germination growth,and drought tolerance in Arabidopsis thaliana.The role of NUA in response to ABA depends on SnRK2.2 and SnRK2.3 for seed germination and on SnRK2.6 for drought.NUA does not directly inhibit the phosphorylation of these SnRK2s or affects their abundance.However,the NUA-interacting protein EARLY IN SHORT DAYS 4(ESD4),a SUMO protease,negatively regulates ABA signaling by directly interacting with and inhibiting SnRK2 phosphorylation and protein levels.More importantly,we demonstrated that SnRK2.6 can be SUMOylated in vitro,and ESD4 inhibits its SUMOylation.Taken together,we identified NUA and ESD4 as SnRK2 kinase inhibitors that block SnRK2 activity,and reveal a mechanism whereby NUA and ESD4 negatively regulate plant responses to ABA and drought stress possibly through SUMOylation-dependent regulation of SnRK2s. | Ya-Nan Chang Zhijuan Wang Ziyin Ren Chun-Han Wang Pengcheng Wang Jian-Kang Zhu Xia Li Cheng-Guo Duan | 2022 | Journal of Integrative Plant Biology2022，64，11： | 2 |
| 3 | GmYSL7 controls iron uptake,allocation,and cellular response of nodules in soybean显示文摘Iron(Fe)is essential for DNA synthesis,photosynthesis and respiration of plants.The demand for Fe substantially increases during legumesrhizobia symbiotic nitrogen fixation because of the synthesis of leghemoglobin in the host and Fecontaining proteins in bacteroids.However,the mechanism by which plant controls iron transport to nodules remains largely unknown.Here we demonstrate that GmYSL7 serves as a key regulator controlling Fe uptake from root to nodule and distribution in soybean nodules.GmYSL7 is Fe responsive and GmYSL7 transports iron across the membrane and into the infected cells of nodules.Alterations of GmYSL7 substantially affect iron distribution between root and nodule,resulting in defective growth of nodules and reduced nitrogenase activity.GmYSL7 knockout increases the expression of GmbHLH300,a transcription factor required for Fe response of nodules.Overexpression of GmbHLH300 decreases nodule number,nitrogenase activity and Fe content in nodules.Remarkably,GmbHLH300 directly binds to the promoters of ENOD93 and GmLbs,which regulate nodule number and nitrogenase activity,and represses their transcription.Our data reveal a new role of GmYSL7 in controlling Fe transport from host root to nodule and Fe distribution in nodule cells,and uncover a molecular mechanism by which Fe affects nodule number and nitrogenase activity. | Xinying Wu Yongliang Wang Qiaohan Ni Haizhen Li Xuesong Wu Zhanxin Yuan Renhao Xiao Ziyin Ren Jingjing Lu Jinxia Yun Zhijuan Wang Xia Li | 2023 | Journal of Integrative Plant Biology2023，65，1： | 0 |
| 4 | Impact of seepage flow on sediment resuspension by internal solitary waves:parameterization and mechanism显示文摘Sediment incipient motion is the first step in sediment resuspension.Previous studies ignored the effect of seepage flow on the mobility of sediment particles and simplified the seabed surface as a rigid boundary.A flume experiment was designed to innovatively divide the seabed into two parts to control the dynamic response of the seabed and control the seepage conditions.In the experiment,the seabed sediments and the amplitude of internal solitary waves(ISWs)were changed to compare and analyze the impact of seepage flow on the sediment resuspension by shoaling ISWs.Moreover,parametric research and verification were carried out.Results indicate that seepage flow can greatly influence fine sand,promote sediment resuspension,and increase the amount of suspension by two times on average.However,seepage flow had a little effect on the suspension of clayey silt and sandy silt.Besides,seepage force was added to the traditional gravity,drag force,and uplift force,and the parameterization of threshold starting shear stress of coarse-grained sediments was developed.The results of this parameterization were verified,and seepage force was critical to parameterization.The threshold starting shear stress was reduced by 54.6%after increasing the seepage force.The physical mechanism of this process corresponded to the vertical reciprocating transient seepage in and out the seabed interface caused by the wave-induced transient excess pore water pressure.This quantitative study on seepage flow for shear stress of coarse-grained sediments induced by ISWs is critical to geohazard assessment. | Zhuangcai TIAN Chao LIU Ziyin REN Xiujun GUO Mingwei ZHANG Xiuhai WANG Lei SONG Yonggang JIA | 2023 | Journal of Oceanology and Limnology2023，41，2： | 0 |
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