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您的检索式：作者名="Zuo Weiyong"
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| 1 | Effect of N-Carbamylglutamate(NCG) on Growth Performance of ICR Mice显示文摘To understand the effects of self synthesized N-carbamylglutamate(NCG) on growth performance of ICR mice,mice were fed with the basal diet adding with 0.025%,0.05% and 0.1% NCG for 28 d.Effects of different levels of NCG on body weight of mice were observed.The re-sults showed that the addition of NCG in the feed could improve the growth performance of mice,and the growth-promoting effect enhanced with the increasing dose. | Hong Weiming Xu Yi Song Liang Li Peng Zuo Weiyong | 2018 | Animal Husbandry and Feed Science2018，10，2： | 1 |
| 2 | Expression of ChIFN-α in Pichia pastoris and Optimization of Its Expression Condition显示文摘Because yeast codon preferred to synthesize the gene sequence of Ch IFN-α mature peptide,Pichia pastoris expression system was connected into vector p PIC9K,to construct recombinant plasmid p PIC9K-α.After digestion and linearization,the recombinant vector was cloned into P.pastoris GS115,high copy transformant and its methanol utilization type were screened,and its expression conditions were optimized.The results showed that Ch IFN-α successfully expressed in P.pastoris,the expression amount of recombinant protein was the largest after induced expression in 0.5% methanol containing 2% acid hydrolyzed casein at 28℃ for 72 h,and its antiviral activity was the highest. | Wang Yongjuan Dong Yaqing Chen Changchun Zuo Weiyong | 2016 | Animal Husbandry and Feed Science2016，8，5： | 0 |
| 3 | Establishment of Universal RT-PCR Detection Method for Duck Reovirus显示文摘This study was conducted to rapidly detect clinical infection condition of duck reovirus. A pair of specific primers was designed according to gene sequence of σC protein of duck reovirus,and a specific RT-PCR detection method of duck reovirus was established with genome of duck reovirus as template. Different samples were collected from ducks infected by suspected reovirus in Jiangsu Province and subjected to PCR detection. The results showed that the established RT-PCR method could specifically amplify the 438 bp sequence of the conservative region of σC gene,and detec the DNA of duck reovirus as low as 1 gf,with a detection rate of 100%. The RT-PCR method could be used for rapid clinical diagnosis of duck reovirus. | Yongjuan WANG Weiyong ZUO Shanyuan ZHU Anping WANG Shuang WU Weiming HONG Hui LU | 2016 | Agricultural Biotechnology2016，5，6： | 0 |
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