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| 1 | Streptococcus agalactiae:Identification methods,antimicrobial susceptibility, and resistance genes in pregnant women显示文摘BACKGROUND Group B Streptococcus(GBS)is a normal component of the gastrointestinal and genital microbiota in humans and can lead to important infections in newborns.AIM To compare GBS isolation and identification methods as well as to assess the antibiotic susceptibility and to identify resistance genes in GBS strains from pregnant women attended in healthcare services from the city of Vitória da Conquista,in Bahia State,Brazil.METHODS From January 2017 to February 2018,vaginorectal swabs were obtained from 186 participants and the samples were seeded onto chromogenic agar for GBS before and after inoculation in selective broth.Confirmatory identification using 3 CAMP and latex tests was performed in samples with GBS-suggestive colonies.Then,disk diffusion antibiograms were performed in GBS-positive samples,and the detection of the resistance genes ermB,ermTR,mefA,and linB in the clindamycin and/or erythromycin-resistant samples was carried out.RESULTS Thirty-two samples(17.2%)were GBS-positive.The culture in chromogenic agar after sample incubation in selective broth was the most sensitive method(96.9%)for GBS detection.All isolates were susceptible to penicillin,ampicillin,cefotaxime,and vancomycin.Clindamycin resistance was observed in 6 samples(18.8%),while 8 samples(25%)were erythromycin-resistant.All erythromycin and/or clindamycin-resistant GBS strains had negative D-tests.Two strains(25%)presented an M phenotype and 6 isolates(75%)presented a cMLSB phenotype.The ermB gene was identified in 4 samples(44.4%),the mefA gene was also found in 4 samples(44.4%),the ermTR gene was identified in 1 isolate(11.1%),and the linB gene was not found in any isolate.CONCLUSION This study evidenced that the screening for SGB can be performed by means of various methods,including chromogenic media,and that the chemoprophylaxis for pregnant women who cannot use penicillin must be susceptibility-guided. | Fabrícia Almeida Fernandes Santana Tais Viana Ledo de Oliveira Marcelo Barreto de Souza Filho Lucas Santana Coelho da Silva Breno Bittencourt de Brito Fabrício Freire de Melo Cláudio Lima Souza Lucas Miranda Marques Márcio Vasconcelos Oliveira | 2020 | World Journal of Clinical Cases2020，8，18： | 2 |
| 2 | Energetic-protein supplementation in the last 60 days of gestation improves performance of beef cows grazing tropical pastures显示文摘Background: Nutrition is one of the most important factors that affect animal performance, and it therefore also impacts on financial results in beef systems. In this way, finding the best strategy for feeding supplements is of paramount importance. Aiming to evaluate the effect of supplement feeding strategies for beef cows in the last third of gestation, two experiments were conducted. In Experiment 1, 35 pregnant Nellore cows were assigned to a completely randomized design with four treatments: control, which received no supplement; supplementation for the last 30 d of gestation(30-d; 3.0 kg/d); supplementation for the last 60 d of gestation(60-d; 1.5 kg/d); or supplementation for the last 90 d of gestation(90-d; 1.0 kg/d). All supplemented treatments received the same total amount of supplement throughout the experiment: 90 kg(20% of crude protein). A second experiment(Experiment 2) was delineated to evaluate the effects of the amounts offered in Experiment 1 on intake and metabolism. Four multiparous pregnant Nellore cows were assigned to a 4 × 4 Latin square design, with periods of15 d each.Results: There was a linear effect of the number of days of supplementation on calving body weight(BW; P < 0.05)and a quadratic effect on BW change from parturition to d 31 post-calving(P < 0.05), with cows on the 60-d strategy losing less BW post-calving. No difference was found in offspring birth BW(P > 0.10). A significant linear effect on interval from parturition to conception(P < 0.05) was observed, with the highest calving to conception interval being observed in the 90-d strategy. The level of supplementation did not affect forage intake or neutral detergent fiber digestibility(P > 0.10). Nitrogen excreted through urine tended to increase linearly with the level of supplementation(P < 0.10).Conclusion: Providing 1.5 kg of supplement during the last 60 d of gestation improves cow performance after calving, reducing the magnitude of BW lost, and reduces the number of days from calving to re-conception in the following breeding season compared to the usually recommended period of supplementation of 90 d pre-partum. | Aline Gomes da Silva Mário Fonseca Paulino Edenio Detmann Henrique Jorge Fernandes Lincoln da Silva Amorim Román Enrique Maza Ortega Victor Valério de Carvalho Josilaine Aparecida da Costa Lima Felipe Henrique de Moura Mariana Benevides Monteiro Jéssika Almeida Bitencourt | 2018 | Journal of Animal Science and Biotechnology2018，9，1： | 2 |
| 3 | Ser- um cartilage oligomeric matrix protein(COMP) levels in knee os- teoarthritis in a brazilian population : clinical and radiologleal cor- relation 显示文摘 | FERNANDES FA PUCINELLI M LC DA SILVA NP | 2007 | Scand J Rheumatol2007，36，3： | 1 |
| 4 | Isola- tion and characterization of a Pseudomonas aeruginosa from a virgin Brazilian Amazon region with potential to degrade a- trazine显示文摘 | Fernandes A F T da Silva M B P Martins V V | 2014 | Environmental Science and Pollution Research2014，21，13： | 1 |
| 5 | A Electrochemical impedance spectroscopy study during accelerated life test of conductive oxides:Ti/(Ru + Ti + Ce)O2-system显示文摘 | Da Silva L M Fernandes K C De Faria L | 2004 | Electrochimica Acta2004，，49： | 1 |
| 6 | Variants of Human Papillomavirus Types 53, 58 and 66 Identified in Central Brazil显示文摘 | Daniela Marreco Cerqueira Geni Noceti de Lima Camara Márcio Rojas da Cruz Evandro Oliveira Silva Marcelo de Macedo Brígido Luciano Gon?alves de Souza Carvalho Cláudia Renata Fernandes Martins | 2003 | Virus Genes2003，，1： | 1 |
| 7 | Association study of vascular endothelial growth factor and polymorphisms of its gene with ectopic pregnancy显示文摘 | ELITO J JR DAHER S FERNANDES DA SILVA M O | 2010 | Am J Reprod Immunol2010，63，2： | 1 |
| 8 | Biofilms of Enterococcus faecalis and Enterococcus faecium isolated from the processing of ricotta and the control of these pathogens through cleaning and sanitization procedures显示文摘 | da Silva Fernandes M Kabuki DY Kuaye AY | 2015 | Int J Food Microbiol2015，200，： | 1 |
| 9 | Electrochemical impedance spectroscopy study during accelerated life test of conductive oxides: Ti/(Ru + Ti + Ce)O2-system 显示文摘 | DA SILVA L M FERNANDES K C DE FARIA L A | 2004 | Electrochimica Acta2004，49，27： | 1 |
| 10 | Electrochemical reduction of oxygen and hydrogen peroxide catalyzed by a surface copper(Ⅱ)-2,4,6-tris(2-piridil)-1,3,5-triazine complex adsorbed on a graphite electrode显示文摘 | DIAS V L N FERNANDES E N DA SILVA L M S | 2005 | J Power Sources2005，142，： | 1 |
| 11 | Role of alpha-and beta-adrenoreceptors in rat monocyte/macrophage function at rest and acute exercise显示文摘 | da Silva Rossato J Krause M Fernandes A J M | 2014 | J Physiol Biochem2014，70，： | 1 |
| 12 | Elec- trochemical reduction of oxygen and hydrogen peroxide catalyzed by a surface copper(Ⅱ)-2,4,6-tfis(2-piridil)-1,3,5-triazine complex adsorbed on a graphite electrode 显示文摘 | DIAS V L N FERNANDES E N DA SILVA L M S | 2005 | Journal of Power Sources2005，142，12： | 1 |
| 13 | Interpretation of a footing load test on a saprolitic soil from granite显示文摘 | Viana da Fonseca A Matos Fernandes M Silva Cardoso A | 1997 | Géotechnique1997，47，3： | 1 |
| 14 | Epidemiological and clinical characteristics of COVID-19 in a Brazilian public hospital显示文摘BACKGROUND The coronavirus disease 2019(COVID-19)pandemic has become a major health concern worldwide.In that context,the understanding of epidemiological and clinical features associated with the disease and its severity is crucial for the establishment of strategies aimed at disease control and remedy.AIM To describe epidemiological features,signs,symptoms,and laboratory findings among severely ill COVID-19 patients from an inten-sive care unit in northeastern Brazil as well as to evaluate predictor factors for disease outcomes.METHODS This is a prospective single-center study that evaluated 115 patients admitted to the intensive care unit in a northeastern Brazilian hospital.RESULTS The patients had a median age of 65.60±15.78 years.Dyspnea was the most frequent symptom,affecting 73.9%of the patients,followed by cough(54.7%).Fever was reported in approximately one-third of patients and myalgia in 20.8%of the patients.At least two comorbidities were found in 41.7%of the patients,and hypertension was the most prevalent(57.3%).In addition,having two or more comorbidities was a predictor of mortality,and lower platelet count was positively associated with death.Nausea and vomiting were two symptoms that were predictors of death,and the presence of a cough was a protective factor.CONCLUSION This is the first report of a negative correlation between cough and death in severely ill severe acute respiratory syndrome coronavirus 2-infected individuals.The associations between comorbidities,advanced age,and low platelet count and the outcomes of the infection were similar to the results of previous studies,highlighting the relevance of these features. | Fillipe Dantas Pinheiro Luana Weber Lopes Rafael Santos Dantas Miranda Dórea Glauber Rocha Lima Araújo Filipe Antônio França da Silva Breno Bittencourt de Brito Maria Luísa Cordeiro Santos Geovani Moreno Santos Júnior Maria Teresa Araújo de Lorenzo Barcia Renata de Amorim Marques AndréBezerra Botelho Anna Carolina Saúde Dantas Davi Tanajura Costa Adriano Fernandes Teixeira Cláudio Lima Souza Lucas Miranda Marques Guilherme Barretos Campos Márcio Vasconcelos Oliveira Dulciene Maria de Magalhães Queiroz Fabrício Freire de Melo | 2023 | World Journal of Clinical Cases2023，11，8： | 0 |
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