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| 1 | Inhibiting effect of antisense oligonucleotides phosphorthioate on gene expression of TIMP-1 in rat liver fibrosis显示文摘AIM To observe the inhibition of antisenseoligonucleotides (asON) phosphorthioate to thetissue inhibitors metalloproteinase-1 (TIMP-1)gene and protein expression in the liver tissue ofimmunologically induced hepatic fibrosis rats.The possibility of reversing hepatic fibrosisthrough gene therapy was observed.METHODS Human serum albumin (HSA) wasused to attack rats, as hepatic fibrosis model, inwhich asONs were used to block the gene andprotein expressing TIMP-1. According to theanalysis of modulator, structure protein, codingseries of TIMP-1 genome, we designed fourdifferent asONs. These asONs were injected intothe hepatic fibrosis models through coccygealvein. The results was observed by RT-PCR formeasuring TIMP-1 mRNA expression,immunohistochemistry and in situ hybridizationfor collagen Ⅰ, Ⅲ, special staining of collagenfiber, and electron microscopic examination.RESULTS Hepatic fibrosis could last within 363days in our modified model. The expressinglevel of TIMP-1 was high during hepatic fibrosisprocess. It has been proved by theimmunohistochemical and the electronmicroscopic examination that the asONphosphorthioate of TIMP-1 could exactly expressin vivo. The effect of colchicine wasdemonstrated to inhibit the expressing level ofmRNA and the content of collagen Ⅰ, Ⅲ in theliver of experimental hepatic fibrosis rats.However, the electron microscopy research andthe pathologic grading of hepatic fibrosisshowed that there was no significant differencebetween the treatment group and the modelgroup (P>0.05).CONCLUSION The experimental rat model ofhepatic fibrosis is one of the preferable modelsto estimate the curative effect of anti-hepaticfibrosis drugs. The asON phosphorthioate ofTIMP-1 could block the gene and proteinexpression of TIMP-1 in the liver of experimentalhepatic fibrosis rats at the mRNA level. It ispossible to reverse hepatic fibrosis, and it isexpected to study a new drug of anti-hepaticfibrosis on the genetic level. Colchicine has verylimited therapeutic effect on hepatic fibrosis,furthermore, its toxicity and side effects areobvious. | Qing He Nie Yong Qian Cheng Yu Mei Xie Yong Xing Zhou Yi Zhan Cao The Center of Infectious Disease Diagnosis and Treatment of PLA,Tangdu Hospital,Forth Military Medical University,Xi’an 710038,Shaanxi Province,ChinaDr,Qing He Nie graduated from Qinghai Medical College as a doctor in 1983,got master degree at Beijing 302 Army Hospital in 1993,got doctor degree at the Third Military Medical University in 1998,engaged in postdoctoral research at the Fourth Military Medical University from 1998 to 2000,now an associate professor,specialized in clinical and experimental research of infectious diseases,had more than 90 papers published,coauthor of ten books,first author of one book. | 2001 | World Journal of Gastroenterology2001，7，3： | 73 |
| 2 | Alterations in metastatic properties of hepatocellular carcinoma cell following H-ras oncogene transfection显示文摘AIM To demonstrate the relationship betweenH-ras oncogene and hepatocellular carcinoma(HCC) metastasis.METHODS Activated H-ras oncogene wastransfected into SMMC 7721, a cell line derivedfrom human HCC, by calcium phosphatetransfection method. Some metastasis-relatedparameters were detected in vitro, includingadhesion assay, migration assay, expression ofcollagenase ⅣV (c ⅣV ase) and epidermal growthfactor receptor (EGFR).RESULTS The abilities of H-ras-transfected cellclones in adhesion to laminin (LN) or fibronectin(FN), migration, c Ⅳ ase secretion increasedmarkedly, and the expression of EGFR elevatedmoderately. More importantly, these alterationswere consistent positively with the expressionof p21, the protein product of H-ras oncogene.CONCLUSION H-ras oncogene could inducethe metastatic phenotype of HCC cell in vitro toraise its metastatic potential. | Qing Wang~1 Zhi Ying Lin~2 Xiao Li Feng~3 ~1Department of Microbiology,Medical Center of Fudan University.the former Shanghai Medical University,Shanghai 200032,China ~2Liver Cancer Institute,Zhongshan Hospital,Shanghai 200032,China ~3Shanghai Institute of Biochemistry,Academy Sinica,Shanghai 200031,ChinaQing Wang earned master degree from Shanghai Medical University in 1996,now a senior lecturer of microbiology,specialized in the role of oncogcncs on tumor metastasis,having 8 papers published. | 2001 | World Journal of Gastroenterology2001，7，3： | 48 |
| 3 | The New Form of Time-Dependent Mild Slope Equation for Random Waves显示文摘The mild-slope equation derived by Berkhoff (1972), has widely been used in the numerical calculation of refraction and diffraction of regular waves. However, it is well known that the random sea waves has a significant effect in the refraction and diffraction problems. In this paper, a new form of time-dependent mild slope equation for irregular waves was derived with Fade approximation and Kubo’s time series concept. The equation was simplified using WKB method, and simple and practical irregular mild slope equation was obtained. Results of numerical calculations are compared with those of laboratory experiments. | Zhang Yonggang , Li Yucheng Teng Bin Doctor Degree Candidate, The National Key Laboratory of Coastal and Offshore Engineering, Dalian University of Technology, Dalian 116023Professor, The National,Key Laboratory of Coastal and Offshore Engineering, Dalian University of Technology, Dalian 116023Associate Professor, The National Key Laboratory of Coastal and Offshore Engineering, Dalian University of Technology, Dalian 116023 | 1995 | China Ocean Engineering1995，10，4： | 21 |
| 4 | Studies on mechanism of Sialy Lewis-X antigen in liver metastases of human colorectal carcinoma显示文摘INTRODUCTIONSialyl Lewis-X antigen ,correlated with carcinoma, is a group of carbohydrate antigen containing oligosaccharide expressed of embryonic tisue and glycoproteins on cell surface of embryonic tissue[1].The SLeX antigen located on cell surface is synthesized principally by two enzymes ,al ,3fucosyltransfrease and a2, 3sialyctransferase.In adults ,SLeX antigen is expressed principally on the surfaces of granulocytic cells and some tumor cells . | Xiao Wei Li~1 Yan Qing Ding~1 Jun Jie Cai~1 Shao Qing Yang~2 Lian Bing An~3 Dong Fang Qiao~3 ~1Department of Pathology,Nanfang Hospital of the First Military Medical University,Guangzhou 510515,Guangdong Province,China ~2The Northern Hospital of PLA,Shenyang 110015,Liaoning Province,China ~3Department of Electronmicroscopy,First Military Medical University,Guangzhou 510515,Gangdong Province,ChinaDr.Xiao Wei Li graduated from the First Military Medical University with a MM degree in 1999.Physician in Charge of pathology,having 6 papers published. | 2001 | World Journal of Gastroenterology2001，7，3： | 19 |
| 5 | Orthotopic transplantation model of human gastrointestinal cancer and detection of micrometastases显示文摘AIM To establish a relevant animal model ofhuman gastrointestinal cancer, which can beused for repetitive investigations, so as toimprove our understanding and management ofcarcinogenesis and cancer metastasis.METHODS Intact tissues of human colorectaland pancreatic cancers were transplanted innude mice. The biological characteristics of theoriginal and the corresponding transplantedtumors were investigated by HE staining, PASstaining and immunostaining. The metastases inthe livers and lungs of nude mice wereinvestigated by immunostaining withbiotinylated mab KL-1 and by RT-PCR using CK20specific primers.RESULTS There were totally 9 of 16 surgicalspecimens growing in nude mice subcutaneouslyand/.or orthotopically (4 of 6 colorectal and 5 of10 pancreatic cancer). Tumor cell content of thespecimens and freezing of tissue specimens areimportant factors influencing the growth oftransplanted tumor. In the group of fresh tumortissues with greater than 50% tumor cellcontent, the success rate of the transplantationwas 100% (3 cases of pancreatic cancer and 3cases of colorectal cancer). The orthotopicallytransplanted tumors resemble the original tumormorphologically and biologically, including TAAexpression such as CEA byimmunohistochemistry, and CEA level in theserum of mice. Ki-67 labeling index and theexpression of TAA especially K-ras, 17-lA andRA-96, are associated with the potential of tumorgrowth in nude mice. Micrometastases in thelungs and livers of tumor bearing mice can bedetected by immunostaining with biotinylatedmab KL-1 and CK20-specific RT-PCR.CONCLUSION An orthotopic transplantationmodel for human colon and pancreatic cancer innude mice has been set up. We have alsoestablished sensitive detection methods withCK-immunohistochemistry and CK20-RT-PCR tostudy xenotransplanted human cancer and itsmetastatic cancer cells in the liver and lung ofnude mice. This study may be helpful inunderstanding the mechanism of cancermetastasis and in developing new diagnosticmethods and therapeutic strategies formetastases including micrometastases. | Jun Hui Cui~1 Uwe Krueger~2 Doris Henne-Bruns~2 Bemd Kremer~2 Holger Kalthoff~2 ~1Department of General Surgery,First Affiliated Hospital,College of Medicine,Zhejiang University,Hangzhou 310003,Zhejiang Province,China ~2Department of General Surgery,Christian-Albrechts-University,Kiel,GermanyDr.Jun Hui Cui graduated from Zhejiang Medical University in 1984,earned master degree in 1990,studied in the Surgical Department of Kiel University and worked in the Lab of Molecular Oncology of Kiel University from 1994-1997achieved M.D.from Kiel University.Germany,now associate professor of surgery,specialized in colorectal oncology.Adviser of graduated student for master degree,having 20 publications published in key Chinese or English journals. | 2001 | World Journal of Gastroenterology2001，7，3： | 19 |
| 6 | Mycoplasma infections and different human carcinomas显示文摘AIM To explore relationships between human carcinomas and mycoplasma infection.METHODS Monoclonal antibody PD4, which specifically recognizes a distinct protein from mycoplasma hyorhinis, was used to detect mycoplasma infection in different paraffinembedded carcinoma tissues with immunohistochemistry. PCR was applied to amplify the mycoplasma DNA from the positive samples for confirming immunohistochemistry.RESULTS Fifty of 90 cases (56%) of gastric carcinoma were positive for mycoplasma hyorhinis. In other gastric diseases, the mycoplasma infection ratio was 28% (18/49) in chronic superficial gastritis, 30% (14/ 46) in gastric ulcer and 37% (18/ 49) in intestinal metaplasia. The difference is significant with gastric cancer (X2=12.06, P<0.05). In colon carcinoma, the mycoplasma infection ratio was 55.1% (32/58), but it was 20.9% (10/49) in adenomarous polyp (X2=13.46, P<0.005).Gastric and colon cancers with high differentiation had a higher mycoplasma infection ratio than those with low differentiation (P< 0.05). Mycoplasma infection in esophageal cancer, lung cancer, breast cancer and glioma was 50.9% (27/53), 52.6% (31/ 59), 39.7%(25/63) and 41% (38/91), respectively. The mycoplasma DNA was successfully amplified with the DNA extracted from the cancer tissues that were positive for mycoplasma infection (detected with antibody PD4).CONCLUSION There was high correlation between mycoplasma infection and different cancers, which suggests the possibility of an association between the two. The mechanism involved in oncogenesis by mycoplasma remains unknown. | Su Huang Ji You Li Jan Wu Lin Meng Cheng Chao Shou Beijing Institute for Cancer Research, Peking University School of Oncology, Beijing 100034. ChinaSu Huang, received B. A from Jiangxi Medical College of China in 1994. Now she is a graduate student pursuing a Ph. D degree at the Peking University School of Oncology. | 2001 | World Journal of Gastroenterology2001，7，2： | 18 |
| 7 | Gastrin,somatostatin,and experimental disturbance of the gastrointestinal tract in rats显示文摘INTRODUCTIONThe field of gastrointestinal hormones has expanded at a dizzying rate[1-4].Gastrointestinal hormones as regulatory peptides that appear to be major components of bodily integration and have important regulatory actions on physioligical function of the gastrointestinal tract .The successful isolation of some gastrointestinal hormones and the development of sensitive methods for their detection have led to the unexpected finding that they also exist in the brain . | Yong Li Yao~1 Bo Xu~2 Wan Dai Zhang~1 Yu Gang Song~1 ~1Institute of Gastrointestinal Diseases,Nanfang Hospital,First Military Medical University,Guangzhou 510515,Guangdong Province,China ~2Department of Orthopedics Nanfang Hospital,First Military Medical University,Guangzhou 510515,Guangdong Province,ChinaYong Li Yao graduated from First Military Mcdical University with a master degree in 1998.She now works in the Institute of Gastrointestinal Diseases,Nanfang Hospital as a doctoral candidate majoring gastrointestinal diseases and gastrointestinal hormones,she has published 8 papers. | 2001 | World Journal of Gastroenterology2001，7，3： | 12 |
| 8 | Transretinoic acid inhibits rats gastric epithelial dysplasia induced by N-methyi-N-nitro-N-nitrosoguanidine:influences on cell apoptosis and expression of its regulatory genes显示文摘INTRODUCTIONGastric epithelial dysplasia (GED) hypothetically is a straight-forward concept: dysplastic epithelium replacing the normal gastric epithelium of the stomach [1].In the stomach ,like any other segment of the gut ,it is defined as an unequivocal non-invasive epithelial change[2,3].The observation of gastric dysplasia as a cancerous lesion was recognized over a century ago ,but it is only after the advent of gastroscopy that its clinical significance has been stressed[4-7]. | Ru Tao Cui~1 Gan Cai~2 Zhao Bao Yin~(2*) Yong Cheng~2 Qiu Hong Yang~1 Tao Tian~(3#) ~1Liver Center,Beijing Friendship Hospital Affiliated to CapitalUniversity of Medical Sciences~2Department of Gastroenterology,Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine,Shanghai China and Department of Pathophysiology.Shanghai Medical University.Shanghai,China ~3Pathological Department,Shanghai Medical University (?)Now working at UCLA,USA ~#Now working at University of Michigan,USADr.Ru Tao Cui graduated and got the bachelor degree from Shandong University of Traditional Chinese Medicine in 1994,got doctor degree from Shanghai University of Traditional Chinese Medicine in 2000,now studying as a postdoc,majoring gastroenterology,having 29 papers published. | 2001 | World Journal of Gastroenterology2001，7，3： | 8 |
| 9 | Optical PPM-CDMA Communication System with Optical Hard Limiter显示文摘An optical PPM CDMA communication system with an optical hard limiter in front of the correlator of a receiver is proposed in order to reduce multi user interference. The performance of the system is evaluated when the effects of both multi user interference and photo detector shot noise are considered. An upper bound on the bit error rate is derived. The comparison of the performance of the above receiver with that of the optical PPM CDMA system without an optical hard limiter is made. Simulation results show that the performance of the system with an optical hard limiter is superior to that of the conventional one. | LI Xiao bin,\ ZHAO Xiao hui Biography:\ LI Xiao bin (1966-), female, from Shandong Province, Master degree, interested in the research of OCDMA communications.(Department of Telecommunication Engineering, Jilin University, Changchun 130012, P. R. Chin | 2001 | The Journal of China Universities of Posts and Telecommunications2001，8，1： | 8 |
| 10 | Rapid detection of sepsis complicating acute necrotizing pancreatitis using polymerase chain reaction显示文摘INTRODUCTIONAcute narcotizing pancreatitis usually takes a severe clinical course and is associated with multiple organ dysfunction .With the further understanding of pathophysiological events of acute pancreatisis and the therapeutic measuses taken by the clinicians ,the patients can pass through the critical carry stages ,and then the septic complication caused by rtanslocated bacteria, mostly gram-negative microbes from the intestines ensues[1]. | Wei Zhong Zhang1 Tian Quan Han2 Yao Qing Tang2 Sheng Dao Zhang2 1Department of Surgery. Huangyan First Hospital, Huangyan 318020, Zhejiang Province. China 2Department of Surgery. Ruijin Hospital. Shanghai Second Medical University. Shanghai 200025. ChinaDr. Wei Zhong Zhang, graduated from Shanghai Second MedicalUniversity receiving master degree of surgery in 1999 he is devoted to basic and clinical investigation on severe acute pancreatitis and has one paper published. | 2001 | World Journal of Gastroenterology2001，7，2： | 7 |
| 11 | 七年制高等医学教育教学质量与学位授予质量检查总结报告显示文摘Organized by the Higher Education Department of the State Education Commission and theAcademic Degree Committee of the State Council,an inspection and evaluation of the instructionand academic degree award of 7-year program in clinical medicine and stomatological medicine of15 medical colleges and universities had been conducted for 30 days by 79 medical education expertsand scholars.The result shows that 42l graduates possess the designed qualifications in thecomprehensive quality morally,intellectually and physically.The experience of running 7-yearprogram has promoted the educational reform and development with an overall improvement ofeducational quality of these medical colleges and universities. | The Inspection Party of the Instruction and Academic Degree Award Quality of 7-Year Higher Medical Education Program | 1996 | 中国高等医学教育1996，，2： | 4 |
| 12 | A Study of the Characteristics of Consumers' Behavior under the Web Environmen显示文摘The development of Internet has made shopping online possible. But while we are focusing ourselves on the environment design, plan for the information structures and items or business scales, another important factor has been ignored, that is, the changes of the characteristics of consumers behavior under the web environment. Because we are not able to make the exact online marketing strategy according to the changes of consumers behavior, and although many online shops have been opened, we are not able to get big sales. This article emphasises the analysis of the environment for on line transactions, attampts to investigate the basic modes for information movement and on line shopping of those consumers under the web environment, study the influence on consumers brought by Internet, the shopping styles and their characteristics. | LIU Li (Department of Management Engineering, Nanjing University of Posts and Telecommunications, Nanjing 210003, P.R.China)Biography:\ LIU Li (1958-), male, from Hunan Province, received MS degree in management from Northeast University in 1990, associ | 2001 | The Journal of China Universities of Posts and Telecommunications2001，8，1： | 3 |
| 13 | New Tannins from Lythrum sulicaria L显示文摘NewTanninsfromLythrumSalicariaLMaster'sDegree;Xiu-JingMaSupervisors;Chun-RuJi;Yu-MingWang;Guang-QiangZhang;andYan-ZeLiu(Henan... | Master’s Degree Xiu-Jing MaSupervisors Chun-RuJi Yu-Ming Wang Guang-Qiang Zhang and Yan-Ze Liu(Henan College of Traditional Chinese Medicine ,Zhengzhou 450003)Received December 26,1995,Accepted September 19,1996The crude drug Lythrum salicaria L(Lythr | 1996 | Journal of Chinese Pharmaceutical Sciences1996，5，4： | 2 |
| 14 | Legal Problems Concerning Health Discrimination in Employment显示文摘Year 2003 was regarded by the Chinese media as a year of antidiscrimination by hepatitis virus B (HVB) carriers. The series of events that happened in the year aroused the attention of the whole society to show concern for discrimination against HVB carriers. On April 3, a murder case occurred in Zhejiang Province; on November 20, 1,611 citizens put their signatures to a proposals, demanding review of the provisions on public servant recruitment that bar HVB carriers in 31 provinces and cities and calling for laws to protect HVB carriers. The signed proposal was delivered to the Standing Committee of the National People’s Congress, the Ministry of Health and the Legislative Affairs Office of the State Council. In December, the people’s court in Xinwu District of Wuhu City in Anhui officially accepted the case of the first HVB carrier against the personnel bureau | Ye Jingyi, Professor of the Law School of Beijing University Wei Jing, postgraduate working for the master’s degree at the Law School of Beijing University. | 2004 | The Journal of Human Rights2004，3，4： | 1 |
| 15 | 显示文摘 | Wang Weifan Equitable total coloring of graphs with maximum degree 3 Graphs and Combin | 2002 | 18(2002)2002，，： | 1 |
| 16 | An investigation of the mechanics of roller burnishing through finite element simulation and experiments显示文摘 | Pascale Balland Laurent Tabourot Fabien Degre Vincent Moreau | 2013 | International Journal of Machine Tools and Manufacture2013，，： | 1 |
| 17 | From Raw Materials to Customers:Supply Chain Management in the Service Industry显示文摘 | Jack S Cook Kathy Degree Amie Feroleto | 2001 | SAM Advanced Management Journal2001，66，4： | 1 |
| 18 | Evolutionary fundamentals of thermoreg-ulation显示文摘 | Singer D Why 37 degrees C | | 0，，： | 1 |
| 19 | An experimen- tal study in dogs 显示文摘 | Araujo MG Berglundh T Lindhe J On the dynamics of periodon- tal tissue formation in degree I furcation defects | 1997 | J Clin Periodontol1997，24，10： | 1 |
| 20 | l&heologic&l beh&vior dispersions:Effects of solid content, oh&in extension,&nd temper&ture显示文摘 | Madbouly S A OtaigbeJ U of aqueous polyureth&ne degree of neutraliz&tion N&nd& A K et &l | 2005 | M&cromolecules2005，，38： | 1 |
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