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| 1 | Promotion of spinosad biosynthesis by chromosomal integration of the Vitreoscilla hemoglobin gene in Saccharopolyspora spinosa显示文摘To promote spinosad biosynthesis by improving the limited oxygen supply during high-density fermentation of Saccharopolyspora spinosa, the open reading frame of the Vitreoscilla hemoglobin gene was placed under the control of the promoter for the erythromycin resistance gene by splicing using overlapping extension PCR. This was cloned into the integrating vector pSET152, yielding the Vitreoscilla hemoglobin gene expression plasmid pSET152EVHB. This was then introduced into S. spinosa SP06081 by conjugal transfer, and integrated into the chromosome by site-specific recombination at the integration site ΦC31 on pSET152EVHB. The resultant conjugant, S. spinosa S078-1101, was genetically stable. The integration was further confirmed by PCR and Southern blotting analysis. A carbon monoxide differential spectrum assay showed that active Vitreoscilla hemoglobin was successfully expressed in S. spinosa S078-1101. Fermentation results revealed that expression of the Vitreoscilla hemoglobin gene significantly promoted spinosad biosynthesis under normal oxygen and moderately oxygen-limiting conditions (P<0.01). These findings demonstrate that integrating expression of the Vitreoscilla hemoglobin gene improves oxygen uptake and is an effective means for the genetic improvement of S. spinosa fermentation. | LUO YuShuang KOU XiaoXiao DING XueZhi HU ShengBiao TANG Ying LI WenPing HUANG Fan YANG Qi CHEN HanNa XIA LiQiu | 2012 | Science China(Life Sciences)2012，55，2： | 14 |
| 2 | Phase demodulation method based on a dual-identical-chirped-pulse and weak fiber Bragg gratings for quasi-distributed acoustic sensing显示文摘A phase demodulation method for quasi-distributed acoustic sensing(DAS)systems based on a dual-identicalchirped-pulse and weak fiber Bragg gratings(WFBGs)is proposed.Compared to the use of Rayleigh backscattering light in optical fibers,the implementation of WFBGs can contribute to obtaining an optical signal with a higher signal-to-noise ratio(SNR).The dual-identical-chirped-pulse is generated by a time-delay fiber,and the sinusoidal carrier is generated by the interference between the two chirped pulses reflected by adjacent WFBGs.The phase of the sinusoidal carrier represents the dynamic strain change posed on the sensing fiber.Discrete Fourier transform is used to directly retrieve the phase information.The performance of the phase demodulation from interference signals under different sinusoidal carrier frequencies and SNRs is numerically investigated.The piezoelectric transducer is employed to emulate the sound in the experiment to verify the effectiveness of our method.It is shown that the dynamic strain can be well reconstructed at the end of a 101.64 km fiber when the signal SNR is down to 3.234 d B.Our proposed method enables the application of the long-distance sensing in quasi-DAS systems. | Guanhua Liang Junfeng Jiang Kun Liu Shuang Wang Tianhua Xu Wenjie Chen Zhe Ma Zhenyang Ding Xuezhi Zhang Yongning Zhang Tiegen Liu | 2020 | Photonics Research2020，8，7： | 6 |
| 3 | Effects of ionic strength and temperature on the aggregation and deposition of multi-walled carbon nanotubes显示文摘The aggregation and deposition of carbon nanotubes(CNTs) determines their transport and fate in natural waters.Therefore,the aggregation kinetics of humic-acid treated multi-walled carbon nanotubes(HA-MWCNTs) was investigated by time-resolved dynamic light scattering in NaCl and CaCl_2 electrolyte solutions.Increased ionic strength induced HA-MWCNT aggregation due to the less negative zeta potential and the reduced electrostatic repulsion.The critical coagulation concentration(CCC) values of HA-MWCNTs were 80 mmol/L in NaCl and 1.3 mmol/L in CaCl_2 electrolyte,showing that Ca^(2+) causes more serious aggregation than Na^+.The aggregation behavior of HA-MWCNTs was consistent with Derjaguin-Landau-Verwey-Overbeek theory.The deposition kinetics of HA-MWCNTs was measured by the optical absorbance at 800 ran.The critical deposition concentrations for HA-MWCNT in NaCl and CaCl_2 solutions were close to the CCC values,therefore the rate of deposition cannot be increased by changing the ionic strength in the diffusion-limited aggregation regime.The deposition process was correlated to the aggregation since larger aggregates increased gravitational deposition and decreased random Brownian diffusion.HA-MWCNTs hydrodynamic diameters were evaluated at 5,15 and 25℃.Higher temperature caused faster aggregation due to the reduced electrostatic repulsion and increased random Brownian motion and collision frequency.HA-MWCNTs aggregate faster at higher temperature in either NaCl or CaCl_2electrolyte due to the decreased electrostatic repulsion and increased random Brownian motion.Our results suggest that CNT aggregation and deposition are two correlated processes governed by the electrolyte,and CNT transport is favored at low ionic strength and low temperature. | Lixin Wang Xuezhi Yang Qi Wang Yuxuan Zeng Lei Ding Wei Jiang | 2017 | Journal of Environmental Sciences2017，29，1： | 5 |
| 4 | The role of β18-β19 loop structure in insecticidal activity of Cry1Ac toxin from Bacillus thuringiensis显示文摘The β18-β19 loop in domain III of Cry1Ac toxin is unique among Bacillus thuringiensis Cry proteins. In this study, the role of the loop structure in insecticidal activity of Cry1Ac toxin was investigated. Alanine scanning mutations within the loop were initially generated and most mutants were over-expressed and reduced toxicity at different degrees, except mutant N546A that showed almost 2 times enhanced toxicity against Helicoverpa armigera larvae. Further mutagenic analysis of N546 revealed that a charged amino acid in this position would cause very unfavorable influence on insecticidal activity. In addition, the deletion of N546 led to protein instability because of destruction of the loop integrity. Besides, mutant W544F was much more toxic than W544Y, indicating that hydrophobic nature of the position was important for maintaining the stability and activity of Cry1Ac protein. These findings are the first biological evidence for a structural function of β18-β19 loop in insecticidal activity of the Cry1Ac toxin. | XIA LiQiu WANG FaXiang DING XueZhi ZHAO XinMin FU ZuJiao QUAN MeiFang YU ZiNiu | 2008 | Chinese Science Bulletin2008，53，20： | 4 |
| 5 | Thermo-Economic Modeling and Evaluation of Physical Energy Storage in Power System显示文摘In order to assess the electrical energy storage technologies,the thermo-economy for both capacity-type and power-type energy storage are comprehensively investigated with consideration of political,environmental and social influence.And for the first time,the Exergy Economy Benefit Ratio(EEBR)is proposed with thermo-economic model and applied to three different storage systems in various scenarios,including pumped storage,compressed air energy storage and flywheel energy storage.The impact of the total system efficiency,annual utilization hour,life time,and other key factors are also analyzed.The results show that the EEBRs of pumped storage and compressed air energy storage under peak load shaving condition and flywheel energy storage under frequency modulation service condition are all larger than zero,which means they are all thermo-economically feasible.With extra consideration of political,environmental and social impact,the exergy cost could reduce by about 25%and the EEBR doubles.The sensitivity analysis indicates the similarity and diversity of influence to EEBR between capacity-type and power-type energy storage systems.The former is that energy efficiency is the dominated factor for all three storage systems.The latter is that the difference of exergy benefit mode causes variety in other major factors.For energy-type storage system,like pumped storage and compressed air storage,the peak-to-valley price ratio is very sensitive in energy arbitrage.For power-type storage system,like flywheel storage,the mileage ratio is in leading position in auxiliary service benefit by mileage.In the three cases studied,the pumped storage has the best thermo-economy;the compressed air energy storage is the second,and the flywheel energy storage is the third.The main reason is that the pumped storage has the least non-exergy cost,and flywheel has the most. | HU Shan LIU Chang DING Jie XU Yujie CHEN Haisheng ZHOU Xuezhi | 2021 | Journal of Thermal Science2021，30，6： | 4 |
| 6 | Reducing methane emissions and the methanogen population in the rumen of Tibetan sheep by dietary supplementation with coconut oil显示文摘 | Xuezhi Ding Ruijun Long Qian Zhang Xiaodan Huang Xusheng Guo Jiandui Mi | 2012 | Tropical Animal Health and Production2012，，： | 1 |
| 7 | Homology Modeling of CrylAc Toxin-binding Alkaline Phosphatase Receptor from Helicoverpa armigera and Its Functional Interpretation显示文摘 | Shan, Shiping Xia, Liqiu Ding, Xuezhi Zhang, Youming Hu, Shengbiao Sun, Yunjun Yu, Ziquan Han, Lizhen | 2011 | Chinese Journal of Chemistry2011，29，3： | 1 |
| 8 | Improving the insecticidal activity by expression of a recombinant cry1Ac gene with chitinase-encoding gene in acrystalliferous Bacillus thuringiensis显示文摘 | Ding Xuezhi Luo Zhaohui Xia Liqiu | | 0，，05： | 1 |
| 9 | The preparation of Zn 2+ -doped TiO 2 nanoparticles by sol–gel and solid phase reaction methods respectively and their photocatalytic activities显示文摘 | Guoguang Liu Xuezhi Zhang Yajie Xu Xinshu Niu Liqing Zheng Xuejun Ding | 2004 | Chemosphere2004，，9： | 1 |
| 10 | G-morphic modules显示文摘 | YAN XIaoguang CHU Maoquan DING Xuezhi | 2006 | J Anhui Normal University: Nature Science2006，29，5： | 1 |
| 11 | Efficiency of in vitro embryo production of yak (Bos grunniens) cultured in different maturation and culture conditions显示文摘 | Xian Guo Xuezhi Ding Jie Pei Pengjia Bao Chunnian Liang Min Chu Ping Yan | 2012 | Journal of Applied Animal Research2012，，4： | 1 |
| 12 | G-morphic Modules显示文摘 | YAN Xiaoguang CHU Maoquan DING Xuezhi | 2006 | Joumel of Anhui Normal University(Natural Science)2006，29，5： | 1 |
| 13 | Detection of chromosomally located and plasmid-borne genes on 20?kb DNA fragments in parasporal crystals from Bacillus thuringiensis显示文摘 | Yunjun Sun Wei Wei Xuezhi Ding Liqiu Xia Zhiming Yuan | 2007 | Archives of Microbiology2007，，4： | 1 |
| 14 | Identification of cry-Type Genes on 20-kb DNA Associated with Cry1 Crystal Proteins from Bacillus thuringiensis显示文摘 | Liqiu Xia Yunjun Sun Xuezhi Ding Zujiao Fu Xiangtao Mo He Zhang Zhiming Yuan | 2005 | Current Microbiology2005，，1： | 1 |
| 15 | Study on Complete Mitochondrial Genome of Oula Sheep(Ovis aries)显示文摘Mitochondrial genome has been widely used in species identification and gene conservation.In the present study,the complete mitochondrial genome of Oula sheep(Ovis aries)was determined using next-generation sequencing.This genome was16 618 bp(NCBI accession number:KU575248)and contained 13 protein coding genes,22 transfer RNA genes,two ribosomal RNA genes,and a typical control region.The overall nucleotide composition was 33.7%A,27.4%T,25.8%C,and 13.1%G,with a total A+T content of 61.1%.The phylogenetic analysis of selected sheep breeds showed that Oula sheep were clustered within branch A and originated from approximately 6 ka.This mitochondrial genome will provide valuable information for molecular genetic research of Oula sheep. | Xian GUO Jianbin LIU Yufeng ZENG Xuezhi DING Pengjia BAO Ping YAN Jie PEI | 2017 | Agricultural Science & Technology2017，18，8： | 0 |
| 16 | Analysis on Composition and Content of Glucosinolates in Broccoli Flowers and Leaves显示文摘Glucosinolate composition and content were evaluated in flowers and leaves of 12 different broccoli varieties. The results indicated that there were 9 glucosinolates in broccoli,namely Glucoiberin(IBE); Progoitrin(PRO);Sinigrin(SIN);Glucoraphanin(RAA);Gluconapin(NAP);4-Hydroxyglucobrassicin(4OH);Glucobrassicin(GBC);4-Methoxyglucobrassicin(4ME);Neoglucobrassicin(NEO).Total glucosinolate content in flowers was 1-5 times higher than in leaves. The predominant glucosinolate in broccoli was glucoraphanin. | Guangmin LIU Yue MA Yunhua DING Liping HU Xuezhi ZHAO Hongju HE | 2017 | Asian Agricultural Research2017，9，4： | 0 |
| 17 | Characterization of the Complete Mitochondrial Genome of Przewalski's Gazelle Procapra przewalskii in Bovidae Family of Artiodactyla显示文摘The Przewalski's gazelle,Procapra przewalskii,is one of the most endangered species in China,and is now found only in a single small area around the Lake Qinghai.In this study,the complete mitochondrial genome of P.przewalskii was determined and annotated.The circular genome is 16 547-bp long,containing13 protein-coding genes,22 transfer RNA genes,2 ribosomal RNA genes,and a predicted control region.The overall base composition was 34.0% A,24.7% C,28.3% T,and 13.0% G,with a total A + T content of 62.3%.Phylogenetic analysis of all 19 Bovidae species indicated that P.przewalskii showed a close relationship to Procapra gutturosa.Our results provide a great deal of useful information on further studies for conservation biology of Przewalski's gazelle. | Xian GUO Jie PEI Yuqing ZHOU Pengjia BAO Jianbin LIU Hanzhong JI Xiaoyun WU Xuezhi DING Ping YAN Suonan ZHAO | 2018 | Agricultural Biotechnology2018，7，6： | 0 |
| 18 | Study on the Complete Mitochondrial Genome of Qaidam Cattle(Bos taurus)显示文摘Qaidam cattle(Bos taurus) is an important endemic breed in Northwest China, which is mainly distributed in the northwest of Qinghai-Tibet Plateau. It has strong adaptive ability to plateau and swamp conditions, such as cold tolerance and insect resistance. In this study, the first complete mitochondrial genome of Qaidam cattle was reported. The circular double-stranded genome is 16 340 bp in size, and contains 13 protein-coding genes, 22 transfer RNA genes, two ribosomal RNA genes, and a D-loop region. The overall nucleotide composition is 33.4% A, 27.2% T, 26.0% C and 13.4% G, with a total A +T content of60.6%. The gene order and composition are similar to those of other B. taurus breeds. Molecular phylogenetic analysis indicated that Qaidam cattle was split as an independent clade and nested within Asian cattle breeds. | xian guo lin xiong jianbin liu shirong qi zhaxi zhuoma gabu zengcuo jie pei pengjia bao ping yan xuezhi ding | 2017 | Agricultural Science & Technology2017，18，12： | 0 |
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