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| 1 | NaCl胁迫对甘薯试管苗生长和离子含量的影响(英文)显示文摘[Objective] This study aimed to investigate the salt-tolerance mechanism of sweet potato.[Method]Two sweet potato varieties of Xu 25-2(salt-tolerant cultivar)and Triumph 100(salt-sensitive cultivar)were treated by sodium chloride with the concentration of 0 mmol/L and 100 mmol/L.After 20 days,Na+ content and Na+/K+ ratio in the roots,shoots and leave were determined by the flame photometer,while dry weight and fresh weight of roots,shoots and leave in different varieties were also studied.[Result]The growth of two sweet potato varieties was inhibited under salt stress,so the plant became shorter,leaf and root became fewer,dry weight of roots and leave decreased,but seedlings of Xu 25-2 were inhibited slightly.Furthermore,Na+ content and Na+/K+ ratio in roots,shoots and leaves of two sweet potato varieties increased.Na+ content of salt-tolerant Xu 25-2 was low in roots,shoots and leaves,while Na+ content of salt-sensitive Triumph 100 was high in shoots and leave of seedlings,but the change range of Xu 25-2 was less than that of Triumph 100.[Conclusion]The lower Na+ content and Na+/K+ ratio in leaves under salt stress were the most important characteristics for salt-tolerance of sweet potato varieties. | 高叶 赵术珍 陈敏 宋晓征 王宝山 | 2008 | Agricultural Science & Technology2008，9，5： | 13 |
| 2 | Salt Tolerance in Rice: Focus on Mechanisms and Approaches显示文摘Salt tolerance is an important constrain for rice, which is generally categorized as a typical glycophyte. Soil salinity is one of the major constraints affecting rice production worldwide, especially in the coastal areas. Susceptibility or tolerance of rice plants to high salinity is a coordinated action of multiple stress responsive genes, which also interacts with other components of stress signal transduction pathways. Salt tolerant varieties can be produced by marker-assisted selection or genetic engineering by introducing salt-tolerance genes. In this review, we have updated on mechanisms and genes which can help in transferring of the salt tolerance into high-yielding rice varieties. We have focused on the need for integrating phenotyping, genomics, metabolic profiling and phenomics into transgenic and breeding approaches to develop high-yielding as well as salt tolerant rice varieties. | Inja Naga Bheema Lingeswara REDDY Beom-Ki KIM In-Sun YOON Kyung-Hwan KIM Taek-Ryoun KWON | 2017 | Rice science2017，24，3： | 12 |
| 3 | Physiological Mechanism of Salicylic Acid for Alleviation of Salt Stress in Rice显示文摘Soil salinity is one of the most important problems of crop production in estuarine and coastal zones. Improvement in salt tolerance of major food crops is an important way for the economic utilization of coastal zones. This study proved that the application of salicylic acid(SA) improved the growth and yield under salt stress conditions and investigated its physiological mechanisms for salt tolerance. The investigation on the effect of SA for salt tolerance during germination showed that the decreased rates of germination and growth(in terms of shoot and root lengths) by the salt stress were significantly increased by the SA application(SA + NaCl). The treatment of SA to the high and low saline soils enhanced the growth, yield and nutrient values of rice. The effects of SA on Na^+, K^+ and Cl~– ionic accumulation were traced under salt stress condition by inductively coupled plasma optical emission spectrometry and ion chromatography. It was revealed that the increased accumulation of Na^+ and Clˉ ions by the salt stress were reduced by SA application. An increased concentration of endogenous SA level was detected from the SA-treated rice varieties(ASD16 and BR26) by liquid chromatography electrospray Ionization-tandem mass spectrometry. The activities of antioxidant enzymes such as superoxide dismutase, catalase and peroxidase were increased by salt stress whereas decreased by the SA application. The study proved that the application of SA could alleviate the adverse effects of salt stress by the regulation of physiological mechanism in rice plants. In spite of salt stress, it can be applied to the coastal and estuarine regions to increase the rice production. | D.JINI B.JOSEPH | 2017 | Rice science2017，24，2： | 11 |
| 4 | Overexpression of GmDREB1 improves salt tolerance in transgenic wheat and leaf protein response to high salinity显示文摘The transcription factor dehydration-responsive element binding protein(DREB)is able to improve tolerance to abiotic stress in plants by regulating the expression of downstream genes involved in environmental stress resistance.The objectives of this study were to evaluate the salt tolerance of GmDREB1 transgenic wheat(Triticum aestivum L.)and to evaluate its physiological and protein responses to salt stress.Compared with the wild type,the transgenic lines overexpressing GmDREB1 showed longer coleoptiles and radicles and a greater radicle number at the germination stage,as well as greater root length,fresh weight,and tiller number per plant at the seedling stage.The yield-related traits of transgenic lines were also improved compared with the wild type,indicating enhanced salt tolerance in transgenic lines overexpressing GmDREB1.Proteomics analysis revealed that osmotic-and oxidative-stressrelated proteins were up-regulated in transgenic wheat leaves under salt stress conditions.Transgenic wheat had higher levels of proline and betaine and lower levels of malondialdehyde and relative electrolyte leakage than the wild type.These results suggest that GmDREB1 regulates the expression of osmotic-and oxidative-stress-related proteins that reduce the occurrence of cell injury caused by high salinity,thus improving the salt tolerance of transgenic wheat. | Qiyan Jiang Zheng Hu Hui Zhang Youzhi Ma | 2014 | The Crop Journal2014，2，Z1： | 11 |
| 5 | The landscape of molecular mechanisms for salt tolerance in wheat显示文摘Wheat is one of the most important food crops, and its yield is seriously restricted by high salinity and other abiotic stresses. Many attempts have been made to elucidate the major physiological processes associated with salt tolerance and to identify the genes controlling the processes. In this review, the major role of high-affinity potassium transporter(HKT)genes in enhancing the salt tolerance of wheat is summarized. The link between maintenance of reactive oxygen species(ROS) homeostasis and salt tolerance through a comprehensive study of a wheat introgression line is examined, and the contribution of a set of genes involved in this process is depicted. New research strategies to uncover the mechanisms underlying salt tolerance in wheat based on recent advances in omics will be discussed. | Meng Wang Guangmin Xia | 2018 | The Crop Journal2018，6，1： | 6 |
| 6 | Characterization of a sodium-regulated glutaminase from cyanobacterium Synechocystis sp. PCC 6803显示文摘Glutaminase is widely distributed among microorganisms and mammals with important functions. Lit-tle is known regarding the biochemical properties and functions of the deamidating enzyme glutami-nase in cyanobacteria. In this study a putative glutaminase encoded by gene slr2079 in Synechocystis sp. PCC 6803 was investigated. The slr2079 was expressed as histidine-tagged fusion protein in Es-cherichia coli. The purified protein possessed glutaminase activity, validating the functional assign-ment of the genomic annotation. The apparent Km value of the recombinant protein for glutamine was 26.6 ± 0.9 mmol/L, which was comparable to that for some of other microbial glutaminases. Analysis of the purified protein revealed a two-fold increase in catalytic activity in the presence of 1 mol/L Na+. Moreover, the Km value was decreased to 12.2 ± 1.9 mmol/L in the presence of Na+. These data demon-strate that the recombinant protein Slr2079 is a glutaminase which is regulated by Na+ through in-creasing its affinity for substrate glutamine. The slr2079 gene was successfully disrupted in Synecho-cystis by targeted mutagenesis and the △slr2079 mutant strain was analyzed. No differences in cell growth and oxygen evolution rate were observed between △slr2079 and the wild type under standard growth conditions, demonstrating slr2079 is not essential in Synechocystis. Under high salt stress condition, however, △slr2079 cells grew 1.25-fold faster than wild-type cells. Moreover, the photosyn-thetic oxygen evolution rate of △slr2079 cells was higher than that of the wild-type. To further charac-terize this phenotype, a number of salt stress-related genes were analyzed by semi-quantitative RT-PCR. Expression of gdhB and prc was enhanced and expression of desD and guaA was repressed in △slr2079 compared to the wild type. In addition, expression of two key enzymes of ammonium assimi-lation in cyanobacteria, glutamine synthetase (GS) and glutamate synthase (GOGAT) was examined by semi-quantitative RT-PCR. Expression of GOGAT was enhanced in △slr2079 compared to the wild type while GS expression was unchanged. The results indicate that slr2079 functions in the salt stress re-sponse by regulating the expression of salt stress related genes and might not play a major role in glutamine breakdown in Synechocystis. | ZHOU Jie1, ZHOU JunXia1,2, YANG HaoMeng1, YAN ChengShi1 & HUANG Fang1 1 Key Laboratory of Photosynthesis and Environmental Molecular Physiology, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China 2 Graduate School of Chinese Academy of Sciences, Beijing 100039, China | 2008 | Science China(Life Sciences)2008，51，12： | 5 |
| 7 | Mapping and validation of a dominant salt tolerance gene in the cultivated soybean(Glycine max) variety Tiefeng 8显示文摘Salt is an abiotic stress factor that strongly affects soybean growth and production. A single dominant gene has been shown to confer salt tolerance in the soybean cultivar Tiefeng 8.The objective of the present study was to genetically map the salt-tolerance gene in an F2:3population and a recombinant inbred line(RIL) population derived from a cross between two cultivated soybeans, Tiefeng 8(tolerant) and 85-140(sensitive). The F2:3families and RILs were treated with 200 mmol L-1Na Cl to evaluate salt tolerance. The F2:3population showed 1(42 tolerant): 2(132 segregating): 1(65 sensitive) segregation, indicating a single dominant gene for salt tolerance in Tiefeng 8. A sequence-characterized amplified region(SCAR) marker from a previously identified random amplified polymorphic DNA(RAPD)marker and four insertion/deletion polymorphism(In Del) markers were developed within the mapping region. Using these markers along with SSR markers, the salt-tolerance gene was mapped within 209 kb flanked by SCAR marker QS08064 and SSR marker Barcsoyssr_3_1301 on chromosome 3. Three markers that cosegregated with the salt tolerance gene and SCAR marker QS08064 were used to genotype 35 tolerant and 23 sensitive soybean accessions. These markers showed selection efficiencies of 76.2% to94.2%. The results indicate that these markers will be useful for marker-assisted breeding and facilitating map-based cloning of the salt tolerance gene in soybean. | Rongxia Guan Jiangang Chen Jinghan Jiang Guangyu Liu Ying Liu Lei Tian Lili Yu Ruzhen Chang Li-juan Qiu | 2014 | The Crop Journal2014，2，6： | 5 |
| 8 | Responses of Four Rice Varieties to Elevated CO_2 and Different Salinity Levels显示文摘This study was carried out in 2014 at Isfahan University of Technology, Iran, to evaluate the responses of four rice varieties(Neda, Deylamani, Shiroudi and Domsorkh) to ambient(360 ± 50 μmol/mol) and elevated(700 ± 50 μmol/mol) air carbon dioxide(CO_2) concentrations under four salinity levels(0, 30, 60 and 90 mmol/L Na Cl). There was significant variation among rice varieties in response to elevated CO_2 concentration under the four salinity levels. Under non-saline condition, elevated CO_2 increased the dry weight of Neda, Deylamani and Domsorkh by 8%, 50% and 8%, respectively, but reversely decreased that of Shiroudi by 34%. Increasing CO_2 concentration significantly reduced the negative effects of salinity on Shiroudi, but these effects were even increased in Deylamani and Domsorkh under all the salinity levels and in Neda only under 30 and 60 mmol/L Na Cl. Significant correlations were established between plant dry weight, SPAD value and leaf area under both CO_2 levels. However, this trend was observed only at ambient CO_2 concentration in the presence of soluble carbohydrates. The results revealed the genotype and salinity dependence of the effects of CO_2 concentrations on the rice traits investigated. | Sheidollah KAZEMI Hamid Reza ESHGHIZADEH Morteza ZAHEDI | 2018 | Rice science2018，25，3： | 3 |
| 9 | Expression analysis of a Na^+/H^+ antiporter gene PeNHX1 from Populus euphratica显示文摘Na+/H+ antiporters play an important role in the salt tolerance of a wide variety of plants.Using the rapid amplification of cDNA ends method,a Na+/H+ antiporter gene (PeNHX1) was isolated from Populus euphratica.The deduced amino acid sequence contained 528 amino acid residues with a conserved amiloride-binding domain (77LFFIYLLPPI86) and shared more than 68% identity with that of AtNHX1 from Arabidopsis thaliana.PeNHX1 can confer resistance to Na+,as well as Li+,to (EP432) an Escherichia coli strain deficient in both nhaA and nhaB,thus proving that it is a functional Na+/H+ antiporter.PeNHX1 expression profile in EP432 reflected pH independent manner.PeNHX1 expression was regulated by salt at the transcriptional level.Meanwhile,results demonstrated that transcripts of PeNHX1 in P.euphratica calli showed a salt dependent response,and thus provide a valuable tool for studying signaling and biochemical pathways involved in salt recognition and response in P.euphratica. | YuXia Wu JianQuan Liu | 2009 | Research in Cold and Arid Regions2009，1，6： | 2 |
| 10 | Selection efficiencies for improving drought/salt tolerances and yield using introgression breeding in rice(Oryza sativa L.)显示文摘The backcross(BC) breeding strategy has been increasingly used for developing high yielding varieties with improved abiotic stress tolerances in rice. In this study, 189Huang-Hua-Zhan(HHZ) introgression lines(ILs) developed from three different selection schemes were evaluated for yield related traits under drought stress and non-stress conditions in the target and off-season winter nursery environments to assess the selection efficiency of BC breeding for improving different complex traits, and led us to five important results. The first result indicated that the primary target traits should be selected first in the target environments(TEs) in order to achieve the maximum genetic gain. Secondly, BC breeding for drought tolerance(DT) in rice was almost equally effective by strong phenotypic selection in the main target environments and in the winter-season of Hainan.Thirdly, exploiting genetic diversity in the subspecific gene pools is of great importance for future genetic improvement of complex traits in rice. Fourthly, considerable genetic gain can be effectively achieved by selection for secondary target traits among the ILs with the primary traits. Finally, the developed ILs provide useful materials for future genetic/genomic dissection and molecular breeding of complex traits. | Ying Wang Lubiao Zhang Afif Nafisah Linghua Zhu Jianlong Xu Zhikang Li | 2013 | The Crop Journal2013，1，2： | 2 |
| 11 | Radiation-induced in vitro mutagenesis system for salt tolerance and other agronomic characters in sugarcane(Saccharum officinarum L.)显示文摘Gamma ray-induced in vitro mutagenesis and selection for salt(NaC l) tolerance were investigated in sugarcane(Saccharum officinarum L.). Embryogenic callus cultures were irradiated(10 to 80 Gy) and subjected to in vitro selection by exposure of irradiated callus to NaC l(0, 50, 100,150, 200, and 250 mmol L-1). Increasing NaC l concentrations resulted in growth reduction and increased membrane damage. Salt-selected callus lines were characterized by the accumulation of proline, glycine betaine, and Na+and K+concentration. Higher accumulation of proline and glycine betaine was observed in NaC l stressed callus irradiated at 20 Gy. Na+concentration increased and K+concentration decreased with increasing salt level. Irradiated callus showed50–60% regeneration under NaC l stress, and in vitro-regenerated plants were acclimatized in the greenhouse, with 80–85% survival. A total of 138 irradiated and salt-selected selections were grown to maturity and their agronomic performance was evaluated under normal and saline conditions. Of these, 18 mutant clones were characterized for different agro-morphological characters and some of the mutant clones exhibited improved sugar yield with increased Brix%,number of millable canes, and yield. The result suggest that radiation-induced mutagenesis offers an effective way to enhance genetic variation in sugarcane. | Ashok A.Nikam Rachayya M. Devarumath Akash Ahuja Harinath Babu Mahadeo G.Shitole Penna Suprasanna | 2015 | The Crop Journal2015，3，1： | 2 |
| 12 | OsSRK1,an Atypical S-Receptor-Like Kinase Positively Regulates Leaf Width and Salt Tolerance in Rice显示文摘Receptor-like kinases(RLKs)are important for plant growth,development and defense responses.The S-receptor protein kinases(SRKs),which represent an RLK subfamily,control the selfincompatibility among Brassica species.However,little information is available regarding SRK functions in rice.We identified a gene OsSRK1 encoding an atypical SRK.The transcript of OsSRK1 was induced by abscisic acid(ABA),salt and polyethylene glycol.OsSRK1 localized to the plasma membrane and cytoplasm.Leaf width was increased in OsSRK1-overexpression(OsSRK1-OX)transgenic rice plants,likely because of an increase in cell number per leaf.Furthermore,the expression levels of OsCYCA3-1 and OsCYCD2-1,which encode positive regulators of cell division,were up-regulated in leaf primordium of OsSRK1-OX rice plants relative to those in wild type.Meanwhile,the expression level of OsKRP1,which encodes cell cycle inhibitor,was down-regulated in the OsSRK1-OX plants.Therefore,it is deduced that OsSRK1 regulates leaf width by promoting cell division in the leaf primordium.Additionally,OsSRK1-OX plants exhibited enhanced ABA sensitivity and salt tolerance compared with wild type.These results suggest that OsSRK1 plays important roles in leaf development and salt responses in rice. | ZHOU Jinjun JU Peina ZHANG Fang ZHENG Chongke BAI Bo LI Yaping WANG Haifeng CHEN Fan XIE Xianzhi | 2020 | Rice science2020，27，2： | 2 |
| 13 | Transformation of japonica rice with RHL gene and salt tolerance of the transgenic rice plant显示文摘Overexpression of the yeast HAL2 gene increases salt tolerance of yeast and plant. Rice HAL2-like (RHL) gene was introduced into a japonica rice cultivar HJ19 with Agrobacterium tumefaciens-mediated transformation. Transgenic plants in R0 generation were selected on the principle of GUS-positive, RHL gene PCR-positive and normal growth. Hygromycin-resistant plants of some transgenic lines in R1 generation increased salt tolerance during the seedling and booting stage, being less damaged in the cy-tomembrane and stronger in leaf tissue viability under salt stress during booting period. Southern analysis of transgenic lines tolerant to salt in R1 generation showed that the RHL gene expression cassette had been successfully integrated into rice genome. Moreover, gene engineering breeding methodology and really salt-tolerant rice cultivar were discussed. | Rongtian Li Zhongming Zhang Qifa Zhang | 2002 | Chinese Science Bulletin2002，47，12： | 2 |
| 14 | Identification of Rice Accessions Associated with K+/Na+ Ratio and Salt Tolerance Based on Physiological and Molecular Responses显示文摘The key for rice plant survival under Na Cl salt stress is maintaining a high K^+/Na^+ ratio in its cells. Selection for salt tolerance rice genotypes based on phenotypic performance alone will delay in progress in breeding. Use of molecular markers in tandem with physiological studies will help in better identification of salt tolerant rice accessions. Eight rice accessions along with the check Dongjin were screened using 1/2 Yoshida solution with 50 mmol/L NaCl at the seedling stage. The accessions IT001158, IT246674, IT260533 and IT291341 were classified as salt tolerant based on their K^+/Na^+ ratios. Seventeen SSR markers reported to be associated with K^+/Na^+ ratio were used to screen the accessions. Five SSR markers(RM8053, RM345, RM318, RM253 and RM7075) could differentiate accessions classified based on their K^+/Na^+ ratios. Banding pattern of the accessions was scored compared to the banding pattern of Dongjin. The study differentiated accessions based on their association of K^+/Na^+ ratio with molecular markers which are very reliable. These markers can play a significant role in screening large set of rice germplasms for salt tolerance and also help in identification of high-yielding varieties with better salt tolerance. The salt tolerant accessions can be taken forward into developing better varieties by conventional breeding and exploring genes for salt tolerance. | Inja Naga Bheema Lingeswara REDDY Sung-Mi KIM Beom-Ki KIM In-Sun YOON Taek-Ryoun KWON | 2017 | Rice science2017，24，6： | 2 |
| 15 | Construction of a Genetic Map and Mapping Quantitative Trait Loci for Salt Tolerance During the Vegetative Stage in Tomato by SSR Markers显示文摘Quantitative trait loci (QTLs) contributing to salt tolerance during the vegetative stage in tomato were investigated. A moderately salt-sensitive Lycopersicon esculentum Mill.‘XF 98-7’ was hybridized with a salt-tolerant Lycopersicon pimpinellifolium accession LA2184, and F2 and F3 populations were developed. The F2 population was used for SSR mapping and the F3 families were evaluated for salt tolerance in solution cultures containing 1% NaCl. A LOD score threshold of 2.0 was chosen to identify putative QTLs and to estimate their additive effect and phenotypic variation. Two genomic regions (LEtat003-SSR139, SSR119-SSR17) were detected on chromosome 4 beating significant QTLs for salt tolerance, respectively accounted for 6.03% and 8.01% of the phenotypic variation. The QTL in the marker interval of LEtat003-SSR139 showed significant negative effects, while the other QTL in the marker interval of SSR119-SSR17 showed significant positive effects. The identification of genomic locations with both positive and negative effects on this trait suggests the likelihood of recovering transgressive segregants in progeny derived from these parental lines. Results and its application in developing salt-tolerant tomatoes as molecular markers are discussed in this paper. | 刘杨 陈火英 庄天明 | 2005 | Journal of Shanghai Jiaotong university(Science)2005，10，S1： | 1 |
| 16 | Transcriptome analysis of salt-stress response in three seedling tissues of common wheat显示文摘Xiaoyan 60(XY60) is a new wheat variety bred in the laboratory of Zhensheng Li.After salt treatment, seedlings of XY60 maintain green leaves and produce longer roots than the high yielding cultivar Zhongmai 175(ZM175).To explain these different phenotypes we carried out an RNA-Seq analysis using 12 samples from three tissues of both varieties subjected to salt and control treatments.By comparing data from the salt treated plants with the control, 703, 979, and 1197 differentially expressed genes(DEGs) were detected in new leaves, old leaves, and roots of XY60, respectively.The corresponding numbers for ZM175 were 613, 1401, and 1301.The most significantly enriched Gene Ontology(GO) terms and KEGG pathways were associated with polyunsaturated fatty acid(PUFA) metabolism in both new and old leaves from XY60.They were associated with photosynthesis and energy metabolism in ZM175.The most significantly enriched KEGG pathway in roots of both varieties was “glucosinolate biosynthesis”.In addition, jasmonic acid(JA) concentration in XY60 was higher than in ZM175, although it increased significantly in both varieties following salt treatment.Trends in relative expression levels of AOS, MYC2, and JAZ revealed by qRT-PCR were concordant with those from RNA-Seq.Our results suggest that PUFAs may contribute to salt tolerance in common wheat by enhancing the photosynthetic system and JA-related pathways. | Qiaoling Luo Wan Teng Shuang Fang Hongwei Li Bin Li Jinfang Chu Zhensheng Li Qi Zheng | 2019 | The Crop Journal2019，7，3： | 1 |
| 17 | An isopentyl transferase gene driven by the senescence-inducible SAG12 promoter improves salinity stress tolerance in cotton显示文摘Background:Soil salinity seriously affects cotton growth,leading to the reduction of yield and fiber quality.Recently,genetic engineering has become an efficient tool to increase abiotic stress tolerance in crops.Results:In this study,isopentyl transferase(IPT),a key enzyme involved in cytokinin(CTK) biosynthesis from Agrobacterium tumefaciens,was selected to generate transgenic cotton via Agrobacterium-mediated transformation.A senescence-inducible SAG12 promoter from Arabidopsis was fused with the IPT gene.Ectopic-expression of SAG12::IPT significantly promoted seed germination or seedling tolerance to salt stress.Two IPTtransgenic lines,OE3 as a tolerant line during seed germination,and OE8 as a tolerant line at seedling stage,were selected for further physiological analysis.The data showed that ectopic-expression of SAG12::IPT induced the accumulation of CTKs not only in leaves and roots,but also in germinating seeds.Moreover,ectopic-expressing IPT increased the activity of antioxidant enzymes,which was associated with the less reactive oxygen species(ROS) accumulation compared with control plants.Also,ectopic-expression of IPT produced higher K^+/Na^+ratio in cotton shoot and root Conclusion:The senescence-induced CTK accumulation in cotton seeds and seedlings positively regulates salt stress partially by elevating ROS scavenging capability. | SHAN Yi ZHAO Peng LIU Zhao LI Fangjun TIAN Xiaoli | 2019 | Journal of Cotton Research2019，2，3： | 1 |
| 18 | Evaluating germinability of eight desert halophytes under long-term seed storage: Implications for conservation显示文摘Ex situ conservation in seed banks is a potential complementary conservation strategy for native plant species.It is well established that ex situ seed banking of native wild plants prolongs seed viability and thereby preserves genetic and species diversity for future use.We evaluated ex situ storage potential of eight halophytic species from deserts in the United Arab Emirates(UAE)by studying seed germination.Specifically,we examined the germinability of freshly collected seeds and seeds stored for three years in a seed bank.We also examined the effect of light conditions on fresh and stored seed germination.Fresh seeds of seven of the eight species tested had a higher germination rates under 12/12 h light/dark fluctuations than did those exposed to total darkness.Storage reduced light sensitivity in Halocnemum strobilaceum,Suaeda aegyptiaca,Salsola drummondii and Salsola imbricata,but increased the requirement for light in Arthrocnemum macrostachyum.In Anabasis setifera,storage decreased germination percentage when there was a 12-hour light/dark fluctuation,but increased germination rate when exposed to the dark treatment.Storage significantly reduced germination in both the light/dark and dark treatments in Suaeda vermiculata and S.aegyptiaca.Germination speed also responded differently to storage;whereas Timson's index significantly increased in A.macrostachyum and H.strobilaceum,it significantly decreased for S.drummondii,S.aegyptiaca and S.vermiculata.Germination of these species at a range of temperatures requires further testing;additionally,we strongly suggest that these laboratory findings be complemented by field studies. | Sanjay Gairola Hatem A. Shabana Tamer Mahmoud Ali El-Keblawy Andrea Santo | 2019 | Plant Diversity2019，41，4： | 0 |
| 19 | Screening for Sodium-Salt Tolerance at the Seedling Stage of Rice(Oryza sativa L.)Genotypes from Sudan and South Sudan显示文摘Morpho-physiological and genetic studies of salinity are important in understanding the mechanism of plant adaptation to stressful environment.Eighteen rice genotypes collected from Sudan and South Sudan,which were never tested for salt tolerance,as well as two genotypes(FL478 as tolerant check and IR29 as sensitive check)from the International Rice Research Institute(IRRI),were subjected to salinity stress at seedling stage.Test was carried out in hydroponic system applying electrical conductivity(EC)12 dS/m NaCl using randomized complete block design with three replicates.Most of the genotypes showed sensitivity to salt stress;one genotype PIPANFARY RED2 was moderately sensitive and three genotypes MASURY1,MASURY2 and FL478 were tolerant.Salinity significantly reduced leaf dry weight,shoot dry weight,root dry weigh and biomass production(biomass/plant)by 31%,42%,60%and 47%,respectively.Tolerant genotypes accumulated low amount(2.52 g/100 g dry weight(dwt))of Na^+in the root,whereas highly sensitive genotypes accumulated high amount(3.87 g/100 g dwt)of Na^+.Tolerant genotypes showed less reduction in K^+concentration than the sensitive genotypes.Therefore,they maintained lowest Na^+/K^+ratio in the shoot(1.47%)than in the root(3.69%)compared to the intolerant genotypes(7.49%and 8.49%).The genotypes that showed tolerance to salinity stress can be used as a source of resistance/tolerance in a breeding program for rice improvement in uplands areas in semi-arid condition. | Seif Gasim Ishraka Abuanja Mohamedsalih Dafalla Abdelwahab Abdalla | 2018 | Journal of Agricultural Science and Technology(B)2018，8，6： | 0 |
| 20 | Screening of Transgenic Soybean Materials for Salt Tolerance显示文摘Soybean is an important oil crop in China, which plays critical roles in China grain market. Therefore, researches on salttolerant soybean cultivars are of great significance and important for the sustainable development and utilization of the agricultural land resources. In this study, transgenic GmIPK-II(IPK-II) soybean seedlings from three single lines and 10 GmHAL-I(HAL-I) transgenic lines were provided by Jilin Academy of Agricultural Sciences. Wild type seedlings of soybean cultivars Dongnong 50(DN50) and Huachun 6(HC6) were the controls. Transgenic and wild-type seedlings were evaluated under different salt concentrations. Moreover, three kinds of culture methods and two kinds of salt application methods including 'irrigated from top' and 'permeated from bottom' were discussed. Through observing the changes of phenotype from transgenic and control seedlings under different salt concentrations, the salt tolerance screening evaluation system with high efficiency was initially confirmed, which conditions were as the followings: vermiculite and turfy soil were mixed as 1 : 1. Concentration of NaCl was 150 mmol · L-1. Time of the treatments was 6 days. Finally, 23 transgenic lines of GmIPK-II and GmHAL-I with higher salt-tolerance were obtained, which laid a foundation for researches. | Yang Yu-ying Deng Jing Ma Qiao-cong Zhang Wei-na Zhang Bin-bin | 2019 | Journal of Northeast Agricultural University(English Edition)2019，26，3： | 0 |
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