The effects of human genome DNA on spinal cord neurons of the embryonic mouse in vitro

查看全文 作　　者：杨大莉;梁喆;鞠躬 出　　处：《Journal of Medical Colleges of PLA(China)》索引1999年第14卷第3期,共4页 摘　　要：objective: To study the effects of human genome DNA on the cultured spinal cord neurons of em bryonic mouse. Methods: The human genome DNA was added to the culture medium of the spinal cord neu rons of embryonic mouse. Eight days later, MTT assay, NSE immunocytochemical staining and image analy sis were proformed to examine the viabilities and the neurites lengths of the neurons. Results: The neurite length of the experimental group was significantly Ionger than that of the control group, but no marked dif ference was found between the viabilities of the neurons of the experimental groups and that of the control ones. Conclusiou: Human genome DNA has no effects on the viabilities of the cultured neurons but can pro mote the neurite growth. 关 键 词：human genome DNA CULTURED NEURON NEURITE OUTGROWTH