Mapping loci controlling fatty acid profiles, oil and protein content by genome-wide association study in Brassica napus

查看全文 作　　者：Minqiang [1,2]Tang;Yuanyuan [1]Zhang;Yueying [1]Liu;Chaobo [1,3]Tong;Xiaohui [1]Cheng;Wei [1]Zhu;Zaiyun [2]Li;Junyan [1,3]Huang;Shengyi [1,3]Liu

机构地区：[1]The Key Laboratory of Biology and Genetic Improvement of Oil Crops, The Ministry of Agriculture, Oil Crops Research Institute of Chinese Academy of Agricultural Sciences;[2]National Key Laboratory of Crop Genetic Improvement, National Center of Crop Molecular Breeding Technology, National Center of Oil Crop Improvement (Wuhan), Huazhong Agricultural University;[3]Hubei Collaborative Innovation Center for Green Transformation of Bio-Resources, Faculty of Life Science, Hubei University

出　　处：《The Crop Journal》索引2019年第7卷第2期,共10页

基　　金：supported by the National Key Research and Development Program of China(2016YFD0101007,2016YFD0100305,2018YFD0200904);the National Natural Science Foundation of China(31471536,31770250);the Earmarked Fund for China Agriculture Research System(CARS-12);the Agricultural Science and Technology Innovation Program(ASTIP)of Chinese Academy of Agricultural Sciences 摘　　要：Optimizing the profile and quantity of fatty acids in rapeseed(Brassica napus L.) is critical for maximizing the value of edible oil and biodiesel. However, selection of these complex seed quality traits is difficult before haplotypes controlling their contents are identified. To efficiently identify genetic loci influencing these traits and underlying candidate genes and networks, we performed a genome-wide association study(GWAS) of eight seed quality traits(oil and protein content, palmitic, stearic, oleic, linoleic, eicosenoic and erucic acids content). The GWAS population comprised 370 diverse accessions, which were phenotyped in five environments and genotyped using 60K SNP arrays. The results indicated that oil and protein contents generally showed negative correlations, while fatty acid contents showed positive or negative correlations,with palmitic and erucic acid contents directly affecting oil content. Seven SNPs on five chromosomes were associated with both seed oil and protein content, and five genes orthologous to genes in Arabidopsis thaliana were predicted as candidates. From resequencing data, besides known haplotypes in Bna A.FAE1.a and Bna C.FAE1.a, three accessions harboring a new haplotype conferring moderate erucic acid content were identified. Interestingly, in a haplotype block, one haplotype was associated with high palmitic acid content and low oil content, while the others showed the reverse effects. This finding was consistent with a negative correlation between palmitic acid and oil contents, suggesting historical selection for high oil content. The identification by this study of genetic variation and complex correlations of eight seed quality traits may be beneficial for crop selection strategies. 关键词：BRASSICA NAPUS OIL content Fatty acid profiles Association study Haplotypes

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Mapping loci controlling fatty acid profiles, oil and protein content by genome-wide association study in Brassica napus

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