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Structural shifts in the intestinal microbiota of rats treated with cyclosporine A after orthotropic liver transplantation

查看全文 作　　者：Junjun [1,2,3]Jia;Xinyao [1,2,3]Tian;Jianwen [1,2,3]Jiang;Zhigang [1,2,3]Ren;Haifeng [2,4]Lu;Ning [1,2,3]He;Haiyang [1,2,3]Xie;Lin [1,2,3]Zhou;Shusen [1,2,3]Zheng 高影响力作者 机构地区：[1]Department of Hepatobiliary and Pancreatic Surgery, the First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou 310003, China;[2]Key Laboratory of Combined Multi-Organ Transplantation, Ministry of Public Health;[3]Collaborative Innovation Center for Diagnosis and Treatment ofInfectious Diseases, the First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou 310003, China;[4]State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, the First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou 310003, China高影响力机构 出　　处：《Frontiers of Medicine》索引2019年第13卷第4期,共10页高影响力期刊 基　　金：This work was supported by the China Postdoctoral Science Foundation (No. 2017M610374);Zhejiang Medical Health Technology Project (No. 2019313269);Innovative Research Groups of the National Natural Science Foundation of China (No. 81421062);National Natural Science Foundation of China (No. 81470891);Science and Technology Bureau of Zhejiang Province, China (No. 2016C33145). 摘　　要：Understanding the effect of immunosuppressive agents on intestinal microbiota is important to reduce the mortality and morbidity from orthotopic liver transplantation (OLT). We investigated the relationship between the commonly used immunosuppressive agent cyclosporine A (CSA) and the intestinal microbial variation in an OLT model. The rat samples were divided as follows:(1) N group (normal control);(2) I group (isograft LT, Brown Norway [BN] rat to BN);(3) R group (allograft LT, Lewis to BN rat);and (4) CSA group (R group treated with CSA). The intestinal microbiota was assayed by denaturing gradient gel electrophoresis profiles and by using real-time polymerase chain reaction. The liver histopathology and the alanine/aspartate aminotransferase ratio after LT were both ameliorated by CSA. In the CSA group, the numbers of rDNA gene copies of Clostridium cluster I, Clostridium cluster XIV, and Enterobacteriaceae decreased, whereas those of Faecalibacterium prausnitzii increased compared with the R group. Cluster analysis indicated that the samples from the N, I, and CSA groups were clustered, whereas the other clusters contained the samples from the R group. Hence, CSA ameliorates hepatic graft injury and partially restores gut microbiota following LT, and these may benefit hepatic graft rejection. 关 键 词：MICROBIAL community liver TRANSPLANTATION IMMUNOSUPPRESSIVE agents CYCLOSPORINE A
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