Integrated transcriptome and metabolite profiling highlights the role of benzoxazinoids in wheat resistance against Fusarium crown rot

查看全文 作　　者：Shuonan [1,2]Duan;Jingjing [3]Jin;Yutian [1]Gao;Changlin [4]Jin;Junyi [1]Mu;Wenchao [3]Zhen;Qixin [1]Sun;Chaojie [1]Xie;Jun [1]Ma

机构地区：[1]College of Agronomy and Biotechnology,China Agricultural University,Beijing 100193,China;[2]Institute of Genetics and Physiology,Hebei Academy of Agriculture and Forestry Sciences,Shijiazhuang 050051,Hebei,China;[3]College of Agronomy,Hebei Agricultural University,Baoding 071001,Hebei,China;[4]Hebei Plant Protection and Quarantine General Station,Shijiazhuang 050051,Hebei,China

出　　处：《The Crop Journal》索引2022年第10卷第2期,共11页

基　　金：supported by the National Natural Science Foundation of China(31872865);Central University Basic Scientific Research Program(2018QC158);Program for Modern Agriculture of Hebei Province(494-0402-JBN-S2XB);the Basic Operating Foundation of Hebei Academy of Agriculture and Forestry Sciences(2018110102)。 摘　　要：Fusarium crown rot(FCR), caused by Fusarium spp., is a chronic and severe plant disease worldwide. In the last years, the incidence and severity of FCR in China has increased to the point that it is now considered a threat to local wheat crops. In this study, for the first time, the metabolites and transcripts responsive to FCR infection in the partial resistant wheat cultivar 04 Zhong 36(04 z36) and susceptible cultivar Xinmai 26(XM) were investigated and compared at 20 and 25 days post inoculation(dpi). A total of 443 metabolites were detected, of which 102 were significantly changed because of pathogen colonization.Most of these 102 metabolites belonged to the flavonoid, phenolic acid, amino acid and derivative classes.Some metabolites, such as proline betaine, lauric acid, ribitol, and arabitol, were stably induced by Fusarium pseudograminearum(Fp) infection at two time points and may have important roles in FCR resistance. In line with the reduced seedling height of 04 z36 and XM plants, RNA-seq analysis revealed that FCR infection significantly affected the photosynthesis activities in two cultivars. Furthermore, 15 jasmonate ZIM-domain genes(JAZ) in the significantly enriched ‘regulation of jasmonic acid mediated signaling pathway’ in 04 z36 were down-regulated. The down-regulation of these JAZ genes in 04 z36 may cause a strong activation of the jasmonate signaling pathway. Based on combined data from gene expression and metabolite profiles, two metabolites, benzoxazolin-2-one(BOA) and 6-methoxy-benzoxazolin-2-one(MBOA), involved in the benzoxazinoid-biosynthesis pathway, were tested for their effects on FCR resistance. Both BOA and MBOA significantly reduced fungal growth in vitro and in vivo, and, thus, a higher content of BOA and MBOA in 04 z36 may contribute to FCR resistance. Above all, the current analysis extends our understanding of the molecular mechanisms of FCR resistance/susceptibility in wheat and will benefit further efforts for the genetic improvement of disease resistance. 关键词：Fusarium crown rot WHEAT METABOLITES TRANSCRIPT Benzoxazinoid

相关文献

参考文献（71）
引证文献（5）
耦合文献（304）

参考文献(71)

引证文献(5)

耦合文献(304)

互联网出版许可证新出网证(渝)字10号全国400电话 - 免长途话费

Integrated transcriptome and metabolite profiling highlights the role of benzoxazinoids in wheat resistance against Fusarium crown rot

参考文献(71)

引证文献(5)

耦合文献(304)

用户提示