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| 1 | Tissue and host tropism of influenza viruses:Importance of quantitative analysis显示文摘It is generally accepted that human influenza viruses preferentially bind to cell-surface glycoproteins/ glycolipids containing sialic acids in α2,6-linkage; while avian and equine influenza viruses preferentially bind to those containing sialic acids in α2,3-linkage. Even though this generalized view is accurate for H3 subtype isolates, it may not be accurate and absolute for all subtypes of influenza A viruses and, therefore, needs to be reevaluated carefully and realistically. Some of the studies published in major scientific journals on the subject of tissue tropism of influenza viruses are inconsistent and caused confusion in the scientific community. One of the reasons for the inconsistency is that most studies were quantitative descriptions of sialic acid receptor distributions based on lectin or influenza virus immunohistochemistry results with limited numbers of stained cells. In addition, recent studies indicate that α2,3- and α2,6-linked sialic acids are not the sole receptors determining tissue and host tropism of influenza viruses. In fact, determinants for tissue and host tropism of human, avian and animal influenza viruses are more complex than what has been generally accepted. Other factors, such as glycan topology, concentration of invading viruses, local density of receptors, lipid raft microdomains, coreceptors or sialic acid-independent receptors, may also be important. To more efficiently control the global spread of pandemic influenza such as the current circulating influenza A H1N1, it is crucial to clarify the determinants for tissue and host tropism of influenza viruses through quantitative analysis of experimental results. In this review, I will comment on some conflicting issues related to tissue and host tropism of influenza viruses, discuss the importance of quantitative analysis of lectin and influenza virus immunohistochemistry results and point out directions for future studies in this area, which should lead to a better understanding of tissue and host tropism of influenza viruses. | ZHANG Hong1,2 1 Z-BioMed, Inc., Rockville, MD 20855, USA 2 Department of Respiratory Medicine, Affiliated Hospital of Zunyi Medical College, Zunyi 563003, China | 2009 | Science China(Life Sciences)2009，52，12： | 7 |
| 2 | Construction of human eukaryotic expression plasmid vascular endothelial growth factor 165 and its expression in transfected vascular smooth muscles显示文摘BACKGROUND: The highly specific vascular endothelialgrowth factor (VEGF) induces the growth of vascular en-dothelial cell. This study was to construct the eukaryoticexpression plasmid of vascular endothelial growth factorl65(VEGF165) and observe its expression in vascular smoothmuscles (VSMCs).METHODS: The primers were designed and synthesizedaccording to the gene sequences of human VEGF165. TheVEGF165 gene was obtained from umbilic artery tissue bythe method of RT-PCR, then it was cloned to eukaryoticexpression plasmid pBudCE4.1 by recombination strategy.The eukaryotic expression plasmid named pBudCE4.1/VEGF165 was identified by restriction enzyme digestion,and was sequenced. The pBudCE4.1/VEGF165 was trans-fected into VSMCs by using lipofection. The VEGF165 ex-pression of mRNA and protein was detected by RT-PCRand Western blot respectively.RESULTS: VEGF165 was shown about 576bp by RT-PCR.Sequencing revealed the amplified VEGF165 gene was iden-tical with that in the GeneBank. Restrictive enzyme (HindBam HI) digestion analysis showed that recombinantexpression plasmid pBudCE4. l/tVEGF165 had been con-structed successfully. The expression of VEGF165 at mRNAand protein levels in the transformed VSMCs had beendemonstrated by RT-PCR and Western blot.CONCLUSIONS: The recombinant eukaryotic expressionplasmid pBudCE4.1/VEGF165 has been successfully con-structed and expressed in transformed VSMCs. The presentstudy has laid a foundation for VEGF165 gene therapy ofvascular stenosis in the transplant organ. | Zhong-Jun Wu, Xiao-Hong Yang, Shu-Sen Zheng, Su-Fen Yang and De Shi Organ Transplant Center, First Affiliated Hospital,Zhejiang University School of Medicine, Hangzhou 310003, China Department of General Surgery, Affiliated Hospital of ZunyiMedical College, Zunyi 563003 , China and Department ofVascular Surgery, Chongqing Medical University, Chongqing 400016 , Chi-na | 2004 | Hepatobiliary & Pancreatic Diseases International2004，3，3： | 5 |
| 3 | 显示文摘 | Qing-song JIANG~2 Xie-nan HUANG Gui-zhong YANG~4 Xiao-yan JIANG~5 Qi-xin ZHOU~(2 6) ~2Department of Pharmacology Chongqing Medical University Chongqing 400016 China ~3Department of Pharmacology Zunyi Medical College Zunyi 563003 China ~4Department of Bioc | 2007 | Acta Pharmacologica Sinica2007，，08： | 1 |
| 4 | Sulfur Isotopes Geochemistry of the Nage Cu-Pb Polymetallic Deposit,Southeast Guizhou Province,China显示文摘The Nage Cu-Pb polymetallic deposit is located in the transitional zone of the Yangtze craton and cathaysia,that is the southwest of Jiangnan orogenic belt. The mainly strata are Wentong formation of Mesoproterozoic Sibo group and the Jialu formation(Qbj) ,Wuye formation(Qbw) ,Fanzhao formation(Qbf) and Gongdong formation(Qbg) of the Neoproterozoic Qingbaikou System Xiajiang group. | LONG Xuan-lin1,ZHOU Jia-xi2,HUANG Zhi-long2,WANG Jing-song1,YANG De-zhi1,3,FAN Liang-wu2,BAO Guang-ping2,LIU Yong-kun1(1. The 102 Geological Team,Guizhou Bureau of Exploration and Development of Geology and Mineral Resources,Zunyi 563003,China 2. State key Laboratory of Ore Deposits Geochemistry,Institute of Geochemistry,Chinese Academy of Science,Guiyang 550002,China 3. China University of Geosciences(Wuhan) ,Wuhan 430074,China) evidence. | 2009 | 矿物学报2009，29，S1： | 1 |
| 5 | Lead Isotope and Rock Geochemistry of Zaibian Mafic-Ultramafic Rock,Southeast Guizhou Province,China显示文摘The Zaibian mafic-ultramafic rock is located in the transitional zone of the Yangtze craton and south China fold system,where is the southwest of Jiangnan orogenic belt(Zeng et al.,2003;Wang et al.,in press). | LIU Yong-kun1,ZHOU Jia-xi2,HUANG Zhi-long2,WANG Jing-song1,YANG De-zhi1,3,DING wei2,CHEN Yuan-xing1,BAO Guang-ping2(1. The 102 Geological Team,Guizhou Bureau of Exploration and Development of Geology and Mineral Resources,Zunyi 563003,China 2. State key Laboratory of Ore Deposits Geochemistry,Institute of Geochemistry,Chinese Academy of Science,Guiyang 550002,China 3. China University of Geosciences(Wuhan) ,Wuhan 430074,China) | 2009 | 矿物学报2009，29，S1： | 1 |
| 6 | Effects of pyridoxine on rat testes by means of Sertoli-germ cell co-culture system in vitro显示文摘Objective To investigate the effects of pyridoxine on rat testis in vitro. Method an in vitro system of Sertoli germ cell co culture was applied, the toxic effects of pyridoxine at different concentrations an exposed duration were observed. Results The detachment of germ cells from sertoli cells showed marked dose response and time response relationships with the exposure of pyridoxine. Meanwhile, the characteristic of loosing and ratracting skeleton in the Sertoli cells was found. Conclusions The effects induced by pyridoxine in vitro may reflect damage to Sertoli cells, and testicular cells co culture could be of value for the study of underlying mechanisms of toxic effects of pyridoxine on rat testis. | Huang Houjin (Department of Preventive Medicine,Zunyi Medical College, Zunyi P.R.China 563003) | 2001 | 遵义医学院学报2001，24，1： | 0 |
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