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| 1 | New gene therapy strategies for hepatic fibrosis显示文摘The liver is the largest internal organ of the body, which may suffer acute or chronic injury induced by many factors, leading to cirrhosis and hepatocarcinoma. Cirrhosis is the irreversible end result of fibrous scarring and hepatocellular regeneration, characterized by diffuse disorganization of the normal hepatic structure, regenerative nodules and fibrotic tissue. Cirrhosis is associated with a high co-morbidity and mortality without effective treatment, and much research has been aimed at developing new therapeutic strategies to guarantee recovery. Liver-based gene therapy has been used to downregulate specific genes, to block the expression of deleterious genes, to delivery therapeutic genes, to prevent allograft rejection and to augment liver regeneration. Viral and non-viral vectors have been used, with viral vectors proving to be more efficient. This review provides an overview of the main strategies used in liver-gene therapy represented by non-viral vectors, viral vectors, novel administration methods like hydrodynamic injection, hybrids of two viral vectors and blocking molecules, with the hope of translating findings from the laboratory to the patient′s bed-side. | Adriana M Salazar-Montes Luis D Hernández-Ortega Martha S Lucano-Landeros Juan Armendariz-Borunda | 2015 | World Journal of Gastroenterology2015，21，13： | 12 |
| 2 | Expression and function of renal and hepatic organic anion transporters in extrahepatic cholestasis显示文摘Obstructive jaundice occurs in patients suffering from cholelithiasis and from neoplasms affecting the pancreas and the common bile duct.The absorption,distribution and elimination of drugs are impaired during this pathology.Prolonged cholestasis may alter both liver and kidney function.Lactam antibiotics,diuretics,non-steroidal anti-inflammatory drugs,several antiviral drugs as well as endogenous compounds are classified as organic anions.The hepatic and renal organic anion transport pathways play a key role in the pharmacokinetics of these compounds.It has been demonstrated that acute extrahepatic cholestasis is associated with increased renal elimination of organic anions.The present work describes the molecular mechanisms involved in the regulation of the expression and function of the renal and hepatic organic anion transporters in extrahepatic cholestasis,such as multidrug resistanceassociated protein 2,organic anion transporting polypeptide 1,organic anion transporter 3,bilitranslocase,bromosulfophthalein/bilirubin binding protein,organic anion transporter 1 and sodium dependent bile salt transporter.The modulation in the expression of renal organic anion transporters constitutes a compensatory mechanism to overcome the hepatic dysfunction in the elimination of organic anions. | Anabel Brandoni María Herminia Hazelhoff Romina Paula Bulacio Adriana Mónica Torres | 2012 | World Journal of Gastroenterology2012，18，44： | 5 |
| 3 | Subepicardial endothelial cells invade the embryonic ventricle wall to form coronary arteries显示文摘冠的动脉把血流动带到心肌肉。理解冠的动脉的发展程序提供卓见进冠的动脉疾病的治疗。多重来源被描述了为包括 proepicardium,湾穴 venosus (SV ) ,和心内膜贡献冠的动脉。然而,冠的容器的发展起源仍然在强烈学习下面。我们为学习冠的开发生产了一个新基因工具,一只 AplnCreER 老鼠在开发冠的容器明确地衬里 recombinase,它表示可诱导的 Cre。冠的开发和 AplnCreER 命运跟踪的预定正式就职的定量分析证明 subepicardial endothelial 房间(EC ) 的子孙两个都入侵紧缩的心肌层形成冠的动脉并且在表面上留下生产静脉。我们发现这些 subepicardial EC 是 intramyocardial 的主要来源在发展中的心的冠的容器。在 vitro 将生物的活组织移植于培养基中培养,试金显示这些 subepicardial EC 的多数从 SV 和中庭的心内膜,然而并非从室的心内膜产生。Apln 积极的房间的同种细胞的分析显示单个 subepicardial EC 同等地作出贡献到冠的动脉和静脉。一起,这些数据建议 subepicardial EC 是 intramyocardial 的主要来源在室的冠的动脉墙,和那冠的动脉和静脉在发展中的心有普通起源。 | Xueying Tian Tianyuan Hu Hui Zhang Lingjuan He Xiuzhen Huang Qiaozhen Liu Wei Yu Liang He Zhongzhou Yang Zhen Zhang Tao P Zhong Xiao Yang Zhen Yang Yan Yan Antonio Baldini Yunfu Sun Jie Lu Robert J Schwartz Sylvia M Evans Adriana C Gittenberger-de Groot Kristy Red-Horse Bin Zhou | 2013 | Cell Research2013，23，9： | 3 |
| 4 | Expression of renal Oat5 and NaDC1 transporters in rats with acute biliary obstruction显示文摘AIM: To examine renal expression of organic anion transporter 5(Oat5) and sodium-dicarboxylate cotransporter 1(Na DC1), and excretion of citrate in rats with acute extrahepatic cholestasis.METHODS: Obstructive jaundice was induced in rats by double ligation and division of the common bile duct(BDL group). Controls underwent sham operation that consisted of exposure, but not ligation, of the common bile duct(Sham group). Studies were performed 21 h after surgery. During this period, animals were maintained in metabolic cages in order to collect urine. The urinary volume was determined by gravimetry. The day of the experiment, blood samples were withdrawn and used to measure total and direct bilirubin as indicative parameters of hepatic function. Serum and urine samples were used for biochemical determinations. Immunoblotting for Oat5 and Na DC1 were performed in renal homogenates and brush border membranes from Sham and BDL rats. Immunohistochemistry studies were performed in kidneys from both experimental groups. Total RNA was extracted from rat renal tissue in order to perform reverse transcription polymerase chain reaction. Another set of experimental animals were used toevaluate medullar renal blood flow(m RBF) using fluorescent microspheres.RESULTS: Total and direct bilirubin levels were significantly higher in BDL animals, attesting to the adequacy of biliary obstruction. An important increase in m RBF was determined in BDL group(Sham: 0.53 ± 0.12 m L/min per 100 g body weight vs BDL: 1.58 ± 0.24 m L/min per 100 g body weight, P < 0.05). An increase in the urinary volume was observed in BDL animals. An important decrease in urinary levels of citrate was seen in BDL group. Besides, a decrease in urinary citrate excretion(Sham: 0.53 ± 0.11 g/g creatinine vs BDL: 0.07 ± 0.02 g/g creatinine, P < 0.05) and an increase in urinary excretion of H+(Sham: 0.082 ± 0.03 μmol/g creatinine vs BDL: 0.21 ± 0.04 μmol/g creatinine, P < 0.05) were observed in BDL animals. We found upregulations of both proteins Oat5 and Na DC1 in brush border membranes where they are functional. Immunohistochemistry technique corroborated these results for both proteins. No modifications were observed in Oat5 m RNA and in Na DC1 m RNA levels in kidney from BDL group as compared with Sham ones.CONCLUSION: Citrate excretion is decreased in BDL rats, at least in part, because of the higher Na DC1 expression. Using the outward gradient of citrate generated by Na DC1, Oat5 can reabsorb/eliminate different organic anions of pathophysiological importance. | Anabel Brandoni Adriana Mónica Torres | 2015 | World Journal of Gastroenterology2015，21，29： | 2 |
| 5 | Characterization and expression pattern of zebrafish anti-Mullerian hormone (AMH) relative to sox9a, sox9b, and cyp191a, during gonad development 显示文摘 | Adriana R M Yan Y L Ruth A B | 2005 | Gene Expression Patterns2005，5，： | 1 |
| 6 | Heavy metals in urban soils:A case study from the city of Palermo(Sicily),Italy显示文摘 | Daniela S M Massimo A Adriana B | 2002 | Science of the Total Environment2002，300，5： | 1 |
| 7 | Five-year follow-up of unilateral posteroventral pallidotomy in Parkinson 's disease显示文摘 | Adriana M Eugene C Joseph | 2009 | Surgical Neurology2009，71，5： | 1 |
| 8 | Atherosclerotic changes of extracoronary arteries are associated with the extent of coronary atherosclerosis 显示文摘 | JOHN P CHRISTOS M ADRIANA T | 2000 | Am J Cardiol2000，85，： | 1 |
| 9 | Activated carbons developed from surplus sludge for the removal of dyes from dilute aqueous solutions 显示文摘 | Maria J M Adriana A M Dolors B | 2003 | Chemical Engineering Journal2003，94，： | 1 |
| 10 | Antiulcer activity of Sapindus saponaria L in the rat 显示文摘 | Adriana L A Jayme A A S Elfriede M B | 2002 | Journal of Et hnopharmacology2002，82，： | 1 |
| 11 | Anticancer, antichemotactic and antimicrobial activities of marine sponges collected off the coast of Santa Catarina, southern Brazil显示文摘 | Noel R Monks Clea Lerner Amélia T Henriques Fabiane M Farias Elfrides E.S Schapoval Edna S Suyenaga Adriana B da Rocha Gilberto Schwartsmann Beatriz Mothes | 2002 | Journal of Experimental Marine Biology and Ecology2002，，1： | 1 |
| 12 | A multicentre randomized trial of combined individual and telephone counselling for smoking cessation显示文摘 | Josep M. Ramon Isabel Nerin Araceli Comino Cristina Pinet Francesc Abella José M a Carreras Marta Banque Antoni Baena Sergio Morchon Adriana Jimenez-Muro Adriana Marqueta Assumpcio Vilarasau Raquel Bullon Cristina Masuet-Aumatell | 2013 | Preventive Medicine2013，，： | 1 |
| 13 | The analytical investigation of synthetic street drugs containing cathinone analogs显示文摘 | Amanda M L Philip B S Adriana D A | 2014 | Forensic Sci Int2014，234，： | 1 |
| 14 | Involvement of resident macrophages and mast cells in the writhing nociceptive response induced by zymosan and acetic acid in mice显示文摘 | Ronaldo A Ribeiro Mariana L Vale Sara M Thomazzi Adriana B.P Paschoalato Steve Poole Sergio H Ferreira Fernando Q Cunha | 2000 | European Journal of Pharmacology2000，，1： | 1 |
| 15 | Plant fibre reinforced cement components for roofing显示文摘 | HOLMER SAVASTANO JR VAHAN AGOPYAN ADRIANA M | 1999 | Construction and Building Materials1999，13，8： | 1 |
| 16 | Activated carbon developed from surplus sewage sludge for the removal of dyes from dilute aqueous显示文摘 | Maria J M Adriana Artola M Dolors B | 2003 | Chemical Engineering Journal2003，94，3： | 1 |
| 17 | Effects of arsenite on estrogen receptor-a expression and activity in MCF-7 breast cancer cells显示文摘 | ADRIANA S EIlZABATH P MARY B M | 2000 | Endo2000，141，10： | 1 |
| 18 | Effect of l-arginine on arginase activity in male accessory sex glands of alloxan-treated rats 显示文摘 | JOSE D M ADRIANA S MARTIN P M | 2002 | Reproductive Toxicology2002，16，6： | 1 |
| 19 | Successful bleeding control by a combined conventional surgical approach and video-assisted surgery:a case report 显示文摘 | Cheli M Alberti D Adriana T | 2009 | Ann Thorae Cardiovasc Surg2009，15，4： | 1 |
| 20 | Activated carbons developed from surplus sewage sludge for the removal of dyes from dilute aqueous solutions 显示文摘 | MARTIN M J ADRIANA A BALAGUER M D | 2003 | Chemical Engineering Journal2003，94，： | 1 |
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