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| 1 | Liver insulin-like growth factor 2 methylation in hepatitis C virus cirrhosis and further occurrence of hepatocellular carcinoma显示文摘AIM: To assess the predictive value of the insulinlike growth factor 2 (Igf2) methylation profile for the occurrence of Hepatocellular Carcinoma (HCC) in hepatitis C (HCV) cirrhosis. METHODS: Patients with: (1) biopsy-proven compensated HCV cirrhosis; (2) available baseline frozen liver sample; (3) absence of detectable HCC; (4) regular screening for HCC; (5) informed consent for genetic analysis were studied. After DNA extraction from liver samples and bisulfite treatment, unbiased PCR and DHPLC analysis were performed for methylation analysis at the Igf2 locus. The predictive value of the Igf2 methylation profile for HCC wasassessed by Kaplan-Meier and Cox methods. RESULTS: Among 94 included patients, 20 developed an HCC during follow-up (6.9 ± 3.2 years). The methylation profile was hypomethylated, intermediate and hypermethylated in 13, 64 and 17 cases, respectively. In univariate analysis, two baseline parameters were associated with the occurrence of HCC: age (P = 0.01) and prothrombin (P = 0.04). The test of linear tendency between the three ordered levels of Igf2 methylation and probability of HCC occurrence was significant (Log Rank, P = 0.043; Breslow, P = 0.037; Tarone-Ware, P = 0.039). CONCLUSION: These results suggest that hypomethylation at the Igf2 locus in the liver could be predictive for HCC occurrence in HCV cirrhosis. | Philippe Couvert Alain Carrié Jacques Pariès Jenny Vaysse Audrey Miroglio Antoine Kerjean Pierre Nahon Jamel Chelly Jean-Claude Trinchet Michel Beaugrand Nathalie Ganne-Carrié | 2008 | World Journal of Gastroenterology2008，14，35： | 6 |
| 2 | c-Fos overexpression increases the proliferation of human hepatocytes by stabilizing nuclear Cyclin D1显示文摘AIM: To investigate the effect of stable c-Fos overexpression on immortalized human hepatocyte (IHH) proliferation. METHODS: IHHs stably transfected with c-Fos (IHH-Fos) or an empty vector (IHH-C) were grown in medium supplemented with 1% serum or stimulated with 10% serum. Cell proliferation was assessed by cell counts, 3H-thymidine uptake and fl ow cytometry analyses. The levels of cell cycle regulatory proteins (Cyclin D1, E, A) cyclin dependent kinases (cdk) cdk2, cdk4, cdk6, and their inhibitors p15, p16, p21, p27, to- tal and phosphorylated GSK-3β and epidermal growth factor receptor (EGF-R) were assayed by Western blot- ting. Analysis of Cyclin D1 mRNA levels was performed by reverse transcription-polymerase chain reaction and real-time polymerase chain reaction (PCR) analysis. Stability of Cyclin D1 was studied by cycloheximide blockade experiments. RESULTS: Stable c-Fos overexpression increased cell proliferation under low serum conditions and resulted in a two-fold increase in [3H]-thymidine incorpora- tion following serum addition. Cell cycle analysis by fl ow cytometry showed that c-Fos accelerated the cell cycle kinetics. Following serum stimulation, Cyclin D1 was more abundantly expressed in c-Fos overexpressing cells. Cyclin D1 accumulation did not result from increased transcriptional activation, but from nuclear stabilization. Overexpression of c-Fos correlated with higher nuclear levels of inactive phosphorylated GSK- 3β, a kinase involved in Cyclin D1 degradation and higher levels of EGF-R mRNA, and EGF-R protein compared to IHH-C both in serum starved, and in serum stimulated cells. Abrogation of EGF-R signalling in IHH- Fos by treatment with AG1478, a specif ic EGF-R tyrosine kinase inhibitor, prevented the phosphorylation of GSK-3β induced by serum stimulation and decreased Cyclin D1 stability in the nucleus. CONCLUSION: Our results clearly indicate a positive role for c-Fos in cell cycle regulation in hepatocytes. Importantly, we delineate a new mechanism by which c-Fos could contribute to hepatocarcinogenesis through stabilization of Cyclin D1 within the nucleus, evoking a new feature to c-Fos implication in hepatocellular carcinoma. | Meryem Güller Kahina Toualbi-Abed Agnès Legrand Laurence Michel Alain Mauviel Dominique Bernuau Fanny Daniel | 2008 | World Journal of Gastroenterology2008，14，41： | 6 |
| 3 | A Mammalian microRNA Expression Atlas Based on Small RNA Library Sequencing显示文摘 | Pablo Landgraf Mirabela Rusu Robert Sheridan Alain Sewer Nicola Iovino Alexei Aravin Sébastien Pfeffer Amanda Rice Alice O. Kamphorst Markus Landthaler Carolina Lin Nicholas D. Socci Leandro Hermida Valerio Fulci Sabina Chiaretti Robin Foà Julia Schliwka | 2007 | Cell2007，，7： | 4 |
| 4 | High-energy hybrid femtosecond laser system demonstrating 2×10 PW capability显示文摘We report on a two-arm hybrid high-power laser system(HPLS)able to deliver 2×10 PW femtosecond pulses,developed at the Bucharest-Magurele Extreme Light Infrastructure Nuclear Physics(ELI-NP)Facility.A hybrid frontend(FE)based on a Ti:sapphire chirped pulse amplifier and a picosecond optical parametric chirped pulse amplifier based on beta barium borate(BBO)crystals,with a cross-polarized wave(XPW)filter in between,has been developed.It delivers 10 mJ laser pulses,at 10 Hz repetition rate,with more than 70 nm spectral bandwidth and high-intensity contrast,in the range of 1013:1.The high-energy Ti:sapphire amplifier stages of both arms were seeded from this common FE.The final high-energy amplifier,equipped with a 200 mm diameter Ti:sapphire crystal,has been pumped by six 100 J nanosecond frequency doubled Nd:glass lasers,at 1 pulse/min repetition rate.More than 300 J output pulse energy has been obtained by pumping with only 80%of the whole 600 J available pump energy.The compressor has a transmission efficiency of 74%and an output pulse duration of 22.7 fs was measured,thus demonstrating that the dual-arm HPLS has the capacity to generate 10 PW peak power femtosecond pulses.The reported results represent the cornerstone of the ELI-NP 2×10 PW femtosecond laser facility,devoted to fundamental and applied nuclear physics research. | François Lureau Guillaume Matras Olivier Chalus Christophe Derycke Thomas Morbieu Christophe Radier Olivier Casagrande Sébastien Laux Sandrine Ricaud Gilles Rey Alain Pellegrina Caroline Richard Laurent Boudjemaa Christophe Simon-Boisson Andrei Baleanu Romeo Banici Andrei Gradinariu Constantin Caldararu Bertrand De Boisdeffre Petru Ghenuche Andrei Naziru Georgios Kolliopoulos Liviu Neagu Razvan Dabu Ioan Dancus Daniel Ursescu | 2020 | High Power Laser Science and Engineering2020，8，4： | 4 |
| 5 | Technical problems produced by the Bravo pH test in nonerosive reflux disease patients显示文摘AIM: To evaluate the technical failures of the Bravo pH test in a population with nonerosive gastroesophageal reflux disease. METHODS: Over the course of a year, we prospectively studied a population of 66 nonerosive reflux disease patients who received a Bravo pH test. The number and frequency of all technical failures were documented, quantified and analyzed. RESULTS: A total of 66 patients, with a mean age of 41.7 years, were studied. Technical failures occurred in 15.15% of the sample. The most frequent failures were due to poor data reception (4.5%), early dislodgement (4.5%) and capsule removal (6.1%). CONCLUSION: The Bravo capsule pH test involves a low but non-negligible rate of technical problems, a fact that must always be considered by physicians. | Andrés de Hoyos Edgar Alain Esparza | 2010 | World Journal of Gastroenterology2010，16，25： | 2 |
| 6 | Indirect printing of hierarchical patient-specific scaffolds for meniscus tissue engineering显示文摘The complex meniscus tissue plays a critical role in the knee. The high susceptibility to injury has led to an intense pursuit for better tissue engineering regenerative strategies, where scaffolds play a major role. In this study, indirect printed hierarchical multilayered sca ffolds composed by a silk fibroin (SF) upper layer and an 80/20 (w/w) ratio of SF/ionic-doped β-tricalcium phosphate (TCP) bottom layer were developed. Furthermore, a comparative analysis between two types of sca ffolds pro- duced using di fferent SF concentrations, i.e., 8% (w/v) (Hi8) and 16% (w/v) (Hi16) was performed. In terms of architecture and morphology, the produced sca ffolds presented homogeneous porosity in both layers and no di fferences were observed when comparing both sca ffolds. A decrease in terms of mechanical performance of the sca ffolds was observed when SF concentration decreased from 16 to 8% (w/v). Hi16 revealed a static compressive modulus of 0.66 ± 0.05 MPa and dynamical mechanical properties ranging from 2.17 ± 0.25 to 3.19 ± 0.38 MPa. By its turn, Hi8 presented a compressive modulus of 0.27 ± 0.08 MPa and dynamical mechanical properties ranging from 1.03 ± 0.08 MPa to 1.56 ± 0.13 MPa. In vitro bioactivity studies showed formation of apatite crystals onto the surface of Hi8 and Hi16 bottom layers. Human meniscus cells (hMCs) and human primary osteoblasts were cultured separately onto the top layer (SF8 and SF16) and bottom layer (SF8/TCP and SF16/TCP) of the hierarchical sca ffolds Hi8 and Hi16, respectively. Both cell types showed good adhesion and proliferation as denoted by the live/dead staining, Alamar Blue assay and DNA quanti fication analysis. Subcutaneous implantation in mice revealed weak in flammation and sca ffold’s integrity. The hierarchical indirect printed SF sca ffolds can be promising candidate for meniscus TE sca ffolding applications due their suitable mechanical properties, good biological performance and possibility of being applied in a patient-speci fic approach. | Joao BCosta Joana Silva-Correia Sandra Pina Alain da Silva Morais Sílvia Vieira Hélder Pereira Joao Espregueira-Mendes Rui LReis Joaquim M.Oliveira | 2019 | Bio-Design and Manufacturing2019，2，4： | 2 |
| 7 | Identification and detection of Actinobacillus pleuropneumoniae by PCR based on the gene apxIVA显示文摘 | Alain S Steven P Djordjevic | 2001 | Vet Res2001，79，： | 1 |
| 8 | A novel colorimetric test to study the susceptibility of human cytomegalovirus DNA polymerase to foscarnet显示文摘 | Alain S Scieux C | 2005 | Pathol Biol2005，53，8： | 1 |
| 9 | Effect of neurotropin on cerebral edema,calcium and other elements in mice subarachnoidally injected with carrageenan显示文摘 | Pierre S Alain K L Stijn V L | 1995 | Euro J Pharmacology1995，274，13： | 1 |
| 10 | Molecular mecha- nisms of pancreatitis : Current opinion 显示文摘 | Alain V Jeremy S W Minoti V A | 2008 | 2I Gastroen- terol Hepatol2008，23，9： | 1 |
| 11 | Timing and surgical management of neonatal testicular torsion 显示文摘 | Orehidee D Alain O Dickens S | 2010 | J Pediatr Surg2010，45，5： | 1 |
| 12 | Sedimentary responses to the Pleistocene climatic variations recorded in the South China Sea显示文摘 | Sébastien Boulay Christophe Colin Alain Trentesaux Stéphane Clain Zhifei Liu Christine Lauer-Leredde | 2007 | Quaternary Research2007，，1： | 1 |
| 13 | Spinal Metastases:indications for and resuits of percutaneous injection of acrylic surgical cement显示文摘 | Alain W M Jacques C Jean M S | 1996 | Radiology1996，199，3： | 1 |
| 14 | On-line NIR Analysis and Advanced Control Improving Gasoline Blending显示文摘 | Alain E Michel S Sebastien O | 1994 | Oil&Gas1994，92，42： | 1 |
| 15 | Simulation, fabrication and testing of Bulk Micromachined 6H-SiC High-g Piezoresistive Accelerometer 显示文摘 | Andrew R Atwelll Robert S Okojie Kevin T Kornegay Scott L Roberson Alain Beliveau | 2003 | Sensors and Actuators A: Physics (S0924-4247)2003，104，1： | 1 |
| 16 | Reproducibility of blood tests of liver fibrosis in clinical practice显示文摘 | Paul Calès Pascal Veillon Anselme Konaté Elisabeth Mathieu Catherine Ternisien Alain Chevailler Alban Godon Yves Gallois Fran?oise Joubaud Isabelle Hubert-Fouchard Frédéric Oberti Stéphane Réaud Gilles Hunault Fran?oise Mauriat Fran?oise Lunel-Fabiani | 2007 | Clinical Biochemistry2007，，1： | 1 |
| 17 | Review of recent research into cellulosic whiskers,their properties and their application in nanocomposite field显示文摘 | Azizi S Fannie A Alain D | | 0，，02： | 1 |
| 18 | A Mammalian microRNA Expression Atlas Based on Small RNA Library Sequencing显示文摘 | Pablo Landgraf Mirabela Rusu Robert Sheridan Alain Sewer Nicola Iovino Alexei Aravin Sébastien Pfeffer Amanda Rice Alice O. Kamphorst Markus Landthaler Carolina Lin Nicholas D. Socci Leandro Hermida Valerio Fulci Sabina Chiaretti Robin Foà Julia Schliwka | 2007 | Cell2007，，7： | 1 |
| 19 | Ischemic injury to rat forebrain mitochondria and cellular calcium homeostasis显示文摘 | Michele A S Jhon Z Alain Y F | 1992 | Biochim Biophys Acta1992，1134，： | 1 |
| 20 | Heavy metal mobility assessment in sediments based on a kinetic approach of the EDTA extraction:Search for optimal experimental conditions显示文摘 | David F Alain B Eduarda S | 2002 | Analytica Chi mica Acta2002，，459： | 1 |
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