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| 1 | New insights into estrogenic regulation of O^6-methylguanine DNA-methyltransferase (MGMT) in human breast cancer cells: Co-degradation of ER-α and MGMT proteins by fulvestrant or O^6-benzylguanine indicates fresh avenues for therapy显示文摘Endocrine therapy using estrogen receptor-α(ER-α) antagonists for attenuating horm2one-driven cell proliferation is a major treatment modality for breast cancers.To exploit any DNA repair deficiencies associated with endocrine therapy,we investigated the functional and physical interactions of ER-α with O^6-methylguanine DNA methyltransferase(MGMT),a unique DNA repair protein that confers tumor resistance to various anticancer alkylating agents.The ER-α-positive breast cancer cell lines(MCF-7,T47D) and ER- negative cell lines(MDAMB-468,MDAMB-231),and established inhibitors of ER-α and MGMT,namely,ICI-182,780(Faslodex) and O^6-benzylguanine,respectively,were used to study MGMT- ER interactions.The MGMT gene promoter was found to harbor one full and two half estrogen-responsive elements(EREs) and two antioxidant-responsive elements(AREs).MGMT expression was upregulated by estrogen,downregulated by tamoxifen in Western blot and promoter-linked reporter assays.Similarly,both transient and stable transfections of Nrf-2(nuclear factor-erythroid 2-related factor-2)increased the levels of MGMT protein and activity 3 to 4-fold reflecting novel regulatory nodes for this drugresistance determinant.Of the different ER-α antagonists tested,the pure anti-estrogen fulvestrant was most potent in inhibiting the MGMT activity in a dose,time and ER-α dependent manner,similar to O^6-benzylguanine.Interestingly,fulvestrant exposure led to a degradation of both ER-α and MGMT proteins and O^6-benzylguanine also induced a specific loss of ER-α and MGMT proteins in MCF-7 and T47 D breast cancer cells with similar kinetics.Immunoprecipitation revealed a specific association of ER-α and MGMT proteins in breast cancer cells.Furthermore,silencing of MGMT gene expression triggered a decrease in the levels of both MGMT and ER-α proteins.The involvement of proteasome in the drug-induced degradation of both proteins was also demonstrated.Fulvestrant enhanced the cytotoxicity of MGMT-targeted alkylating agents,namely,temozolomide and BCNU by 3 to 4-fold in ER-α positive cells,but not in ER-negative cells.We conclude that MGMT and ER-α proteins exist as a complex and are co-targeted for ubiquitin-conjugation and subsequent proteasomal degradation.The findings offer a clear rationale for combining alkylating agents with endocrine therapy. | Ameya Paranjpe Nathan I. Bailes Santhi Konduri George C. Bobustuc Francis Ali-Osman Mohd. A. Yusuf Surendra R. Punganuru Hanumantha Rao Madal Debasish Basak AGM Mostofa Kalkunte S. Srivenugopa | 2016 | The Journal of Biomedical Research2016，30，5： | 5 |
| 2 | Numerical simulation of immiscible liquid-liquid flow in microchannels using lattice Boltzmann method显示文摘Immiscible kerosene-water two-phase flows in microchannels connected by a T-junction were numerically studied by a Lattice Boltzmann (LB) method based on field mediators.The two-phase flow lattice Boltzmann model was first validated and improved by several test cases of a still droplet.The five distinct flow regimes of the kerosene-water system,previously identified in the experiments from Zhao et al.,were reproduced.The quantitative and qualitative agreement between the simulations and the experimental data show the effectiveness of the numerical method.The roles of the interfacial tension and contact angle on the flow patterns and shapes of droplets were discussed and highlighted according to the numerical results based on the improved two-phase LB model.This work demonstrated that the developed LBM simulator is a viable tool to study immiscible two-phase flows in microchannels,and such a tool could provide tangible guidance for the design of various microfluidic devices that involve immiscible multi-phase flows. | YONG YuMei YANG Chao JIANG Yi JOSHI Ameya SHI YouChun YIN XiaoLong | 2011 | Science China Chemistry2011，54，1： | 4 |
| 3 | Coeliac autoimmunity in type I diabetes mellitus显示文摘 | Ameya S. Joshi Premlata K. Varthakavi Nikhil M. Bhagwat Manoj D. Chadha Sachin S. Mittal | 2014 | Arab Journal of Gastroenterology2014，，： | 1 |
| 4 | Polymerization kinetics of rodlike rnolecules under quiescent conditions显示文摘 | J Srinivasalu Gupta Ameya Agge D V Khakhar | 2001 | AIChE J2001，47，1： | 1 |
| 5 | Studies on sootblower jet dynamics and ash deposit removal in industrial boilers显示文摘 | Ameya P Babak E Markus B | 2013 | Fuel Processing Technology2013，105，： | 1 |
| 6 | Degradation of NF-KB, p53 and other regulatory redox- sensitive proteins by thiolconjugating and nitrosylating drugs in human tumor cells 显示文摘 | AMEYA PARANJPE KALKUNTE S SRIVENUGOPAL | 2013 | Carcinogenesis2013，34，5： | 1 |
| 7 | Synthesis and biological activity of N-substituted-3-chloro-2-azeti-dinones显示文摘 | AMEYA A C NANDINI R P | 2007 | Molecules2007，12，： | 1 |
| 8 | Unique interstitial miRNA signature drives fibrosis in a murine model of autosomal dominant polycystic kidney disease显示文摘AIM To delineate changes in miRNA expression localized to the peri-cystic local microenvironment(PLM) in an orthologous mouse model of autosomal dominant polycystic kidney disease(ADPKD)(mcwPkd1^(nl/nl)).METHODS We profiled miRNA expression in the whole kidney and laser captured microdissection(LCM) samples from PLM in mcwPkd1^(nl/nl)kidneys with Qiagen miScript 384 HC miRNA PCR arrays. The three times points used are:(1) post-natal(PN) day 21, before the development of trichrome-positive areas;(2) PN28, the earliest sign of trichrome staining; and(3) PN42 following the development of progressive fibrosis. PN21 served as appropriate controls and as the reference time point for comparison of miRNA expression profiles.RESULTS LCM samples revealed three temporally upregulated miRNAs [2 to 2.75-fold at PN28 and 2.5 to 4-fold(P ≤ 0.05) at PN42] and four temporally downregulated miRNAs [2 to 2.75 fold at PN28 and 2.75 to 5-fold(P ≤ 0.05) at PN42]. Expression of twenty-six miRNAs showed no change until PN42 [six decreased(2.25 to 3.5-fold)(P ≤ 0.05) and 20 increased(2 to 4-fold)(P ≤ 0.05)]. Many critical miRNA changes seen in the LCM samples from PLM were not seen in the contralateral whole kidney.CONCLUSION Precise sampling with LCM identifies miRNA changes that occur with the initiation and progression of renal interstitial fibrosis(RIF). Identification of the target proteins regulated by these miRNAs will provide new insight into the process of fibrosis and identify unique therapeutic targets to prevent or slow the development and progression of RIF in ADPKD. | Ameya Patil William E Sweeney Jr Cynthia G Pan Ellis D Avner | 2018 | World Journal of Nephrology2018，7，5： | 1 |
| 9 | Influence of different wind profiles due to varying atmospheric stability on the fatigue life of wind turbines显示文摘 | Ameya Sathe Wire Bierbooms | 2007 | Journal of Physics2007，75，： | 1 |
| 10 | Prognostic Implications of the High-Sensitive C-Reactive Protein in the Catheter Ablation of Atrial Fibrillation显示文摘 | Yenn-Jiang Lin Hsuan-Ming Tsao Shih-Lin Chang Li-Wei Lo Ta-Chuan Tuan Yu-Feng Hu Ameya R. Udyavar Wen-Chin Tsai Chien-Jung Chang Ching-Tai Tai Pi-Chang Lee Kazuyoshi Suenari Shih-Yu Huang Nguyen Huu Tung Shih-Ann Chen | 2010 | The American Journal of Cardiology2010，，4： | 1 |
| 11 | Restricted isometry of fourier matrices and list decodability of random linear codes显示文摘 | Mahdi Cheraghchi Venkatesan Guruswami Ameya Velingker | 2013 | Proceedings of the ACM-SIAM Symposium on Discrete Algorithms(SODA)2013，，： | 1 |
| 12 | Crowd simulation for emergency response using BDI agents based on immersive virtual reality显示文摘 | AMEYA SHENDARKAR KARTHIK VASUDEVAN SEUNGHO LEE | 2008 | Simulation Modeling Practice and Theory2008，16，9： | 1 |
| 13 | Polymerization kinetics of rodlike molecules under quiescent conditions显示文摘 | J Srinivasalu Gupta Ameya Agge D V Khakhar | 2001 | AIChE J2001，47，1： | 1 |
| 14 | Crowd Simulation for Emergency Response Using BDI Agents Based on Immersive Virtual Reality 显示文摘 | Shendarkar Ameya Vasudevanm Karthik | 2008 | Simulation Modeling Practice and Theory2008，16，： | 1 |
| 15 | Response assessment in metronomic chemotherapy: RECIST or PERCIST?显示文摘 | Archi Agrawal Nilendu Purandare Sneha Shah Ameya Puranik Shripad Banavali Venkatesh Rangarajan | 2014 | Indian Journal of Nuclear Medicine2014，，2： | 1 |
| 16 | Electrophysiological Characteristics and Catheter Ablation in Patients with Paroxysmal Supraventricular Tachycardia and Paroxysmal Atrial Fibrillation显示文摘 | SHIH‐LINCHANG CHING‐TAITAI YENN‐JIANGLIN LI‐WEILO TA‐CHUANTUAN AMEYA R.UDYAVAR HSUAN‐MINGTSAO MING‐ HSIUNGHSIEH YU‐FENGHU SHUO‐JUCHIANG YI‐JENCHEN WANWARANGWONGCHAROEN KWO‐CHANGUENG SHIH‐ANNCHEN | 2007 | Journal of Cardiovascular Electrophysiology2007，，4： | 1 |
| 17 | Unidirectional UWB Array Antenna Using Leaf-shaped Bowtie Elements and Flat Reflector 显示文摘 | ITO Y AMEYA M | 2008 | Electronics Letters2008，44，1： | 1 |
| 18 | How research assisted the roll- out of a mobile agriculture information service 显示文摘 | Purvi Mistry Ameya Samant | 2012 | Interna- tional Journal of Market Research2012，54，5： | 1 |
| 19 | Suppression of diacyl-glycerol acyltransferase-2 (DGAT2), but not DGAT1,with antisenseoligonucleotides reverses diet-induced hepatic steatosis and insulinresistance显示文摘 | Cheol SC David BS Ameya Kulkami | 2007 | J Biol Chem2007，282，22： | 1 |
| 20 | An optimized kinetic model of H 2 /CO combustion显示文摘 | Scott G. Davis Ameya V. Joshi Hai Wang Fokion Egolfopoulos | 2004 | Proceedings of the Combustion Institute2004，，1： | 1 |
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