| 1 | Acoustofluidic separation of cells andparticles显示文摘Acoustofluidics,the integration of acoustics and microfluidics,is a rapidly growing research field that is addressing challenges in biology,medicine,chemistry,engineering,and physics.In particular,acoustofluidic separation of biological targets from complex fluids has proven to be a powerful tool due to the label-free,biocompatible,and contact-free nature of the technology.By carefully designing and tuning the applied acoustic field,cells and other bioparticles can be isolated with high yield,purity,and biocompatibility.Recent advances in acoustofluidics,such as the development of automated,point-of-care devices for isolating sub-micron bioparticles,address many of the limitations of conventional separation tools.More importantly,advances in the research lab are quickly being adopted to solve clinical problems.In this review article,we discuss working principles of acoustofluidic separation,compare different approaches of acoustofluidic separation,and provide a synopsis of how it is being applied in both traditional applications,such as blood component separation,cell washing,and fluorescence activated cell sorting,as well as emerging applications,including circulating tumor cell and exosome isolation. | Mengxi Wu Adem Ozcelik Joseph Rufo Zeyu Wang Rui Fang Tony Jun Huang | 2019 | Microsystems & Nanoengineering2019，5，1： | 2 |
| 2 | Microfluidic methods used in exosome isolation显示文摘Exosomes are important biomarkers for clinical diagnosis.It is critical to isolate secreted exosomes from bodily fluids such as blood,saliva,breast milk,and urine for liquid biopsy applications.The field of microfluidics provides numerous benefits for biosample processing,diagnostics,and prognostics.Several microfluidics-based methods have been employed for the isolation and purification of exosomes in the last ten years.These microfluidic methods can be grouped into two categories based on passive and active isolation mechanisms.In the first group,inertial and hydrodynamic forces are employed to separate exosomes based on their size differences.In the second group,external forcefields are integrated into microfluidic platforms to actively isolate exosomes from other bioparticles.In this paper,the application of microfluidic methods in exosome isolation is discussed,and future perspectives on this field are highlighted. | ADEM OZCELIK OZGE CEVIK | 2023 | BIOCELL2023，47，5： | 0 |
您所在的机构暂未开通此部分的功能，请联系管理员