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| 1 | Colour compound lenses for a portable fluorescence microscope显示文摘In this article,we demonstrated a handheld smartphone fluorescence microscope(HSFM)that integrates dualfunctional polymer lenses with a smartphone.The HSFM consists of a smartphone,a field-portable illumination source,and a dual-functional polymer lens that performs both optical imaging and filtering.Therefore,compared with the existing smartphone fluorescence microscope,the HSFM does not need any additional optical filters.Although fluorescence imaging has traditionally played an indispensable role in biomedical and clinical applications due to its high specificity and sensitivity for detecting cells,proteins,DNAs/RNAs,etc.,the bulky elements of conventional fluorescence microscopes make them inconvenient for use in point-of-care diagnosis.The HSFM demonstrated in this article solves this problem by providing a multifunctional,miniature,small-form-factor fluorescence module.This multifunctional fluorescence module can be seamlessly attached to any smartphone camera for both bright-field and fluorescence imaging at cellular-scale resolutions without the use of additional bulky lenses/filters;in fact,the HSFM achieves magnification and light filtration using a single lens.Cell and tissue observation,cell counting,plasmid transfection evaluation,and superoxide production analysis were performed using this device.Notably,this lens system has the unique capability of functioning with numerous smartphones,irrespective of the smartphone model and the camera technology housed within each device.As such,this HSFM has the potential to pave the way for realtime point-of-care diagnosis and opens up countless possibilities for personalized medicine. | Bo Dai Ziao Jiao Lulu Zheng Hunter Bachman Yongfeng Fu Xinjun Wan Yule Zhang Yu Huang Xiaodian Han Chenglong Zhao Tony Jun Huang Songlin Zhuang Dawei Zhang | 2019 | Light(Science & Applications)2019，8，1： | 3 |
| 2 | Acoustofluidic separation enables early diagnosis of traumatic brain injury based on circulating exosomes显示文摘Traumatic brain injury(TBI)is a global cause of morbidity and mortality.Initial management and risk stratification of patients with TBI is made difficult by the relative insensitivity of screening radiographic studies as well as by the absence of a widely available,noninvasive diagnostic biomarker.In particular,a blood-based biomarker assay could provide a quick and minimally invasive process to stratify risk and guide early management strategies in patients with mild TBI(mTBI).Analysis of circulating exosomes allows the potential for rapid and specific identification of tissue injury.By applying acoustofluidic exosome separation—which uses a combination of microfluidics and acoustics to separate bioparticles based on differences in size and acoustic properties—we successfully isolated exosomes from plasma samples obtained from mice after TBI.Acoustofluidic isolation eliminated interference from other blood components,making it possible to detect exosomal biomarkers for TBI via flow cytometry.Flow cytometry analysis indicated that exosomal biomarkers for TBI increase in the first 24 h following head trauma,indicating the potential of using circulating exosomes for the rapid diagnosis of TBI.Elevated levels of TBI biomarkers were only detected in the samples separated via acoustofluidics;no changes were observed in the analysis of the raw plasma sample.This finding demonstrated the necessity of sample purification prior to exosomal biomarker analysis.Since acoustofluidic exosome separation can easily be integrated with downstream analysis methods,it shows great potential for improving early diagnosis and treatment decisions associated with TBI. | Zeyu Wang Haichen Wang Ryan Becker Joseph Rufo Shujie Yang Brian E.Mace Mengxi Wu Jun Zou Daniel T.Laskowitz Tony Jun Huang | 2021 | Microsystems & Nanoengineering2021，7，2： | 3 |
| 3 | Acoustofluidic separation of cells andparticles显示文摘Acoustofluidics,the integration of acoustics and microfluidics,is a rapidly growing research field that is addressing challenges in biology,medicine,chemistry,engineering,and physics.In particular,acoustofluidic separation of biological targets from complex fluids has proven to be a powerful tool due to the label-free,biocompatible,and contact-free nature of the technology.By carefully designing and tuning the applied acoustic field,cells and other bioparticles can be isolated with high yield,purity,and biocompatibility.Recent advances in acoustofluidics,such as the development of automated,point-of-care devices for isolating sub-micron bioparticles,address many of the limitations of conventional separation tools.More importantly,advances in the research lab are quickly being adopted to solve clinical problems.In this review article,we discuss working principles of acoustofluidic separation,compare different approaches of acoustofluidic separation,and provide a synopsis of how it is being applied in both traditional applications,such as blood component separation,cell washing,and fluorescence activated cell sorting,as well as emerging applications,including circulating tumor cell and exosome isolation. | Mengxi Wu Adem Ozcelik Joseph Rufo Zeyu Wang Rui Fang Tony Jun Huang | 2019 | Microsystems & Nanoengineering2019，5，1： | 2 |
| 4 | Enzymatically-degradable hydrogel coatings on titanium for bacterial infection inhibition and enhanced soft tissue compatibility via a self-adaptive strategy显示文摘Ideal percutaneous titanium implants request both antibacterial ability and soft tissue compatibility.ZnO structure constructed on titanium has been widely proved to be helpful to combat pathogen contamination,but the biosafety of ZnO is always questioned.How to maintain the remarkable antibacterial ability of ZnO and efficiently reduce the corresponding toxicity is still challenging.Herein,a hybrid hydrogel coating was constructed on the fabricated ZnO structure of titanium,and the coating was proved to be enzymatically-degradable when bacteria exist.Then the antibacterial activity of ZnO was presented.When under the normal condition(no bacteria),the hydrogel coating was stable and tightly adhered to titanium.The toxicity of ZnO was reduced,and the viability of fibroblasts was largely improved.More importantly,the hydrogel coating provided a good buffer zone for cell ingrowth and soft tissue integration.The curbed Zn ion release was also proved to be useful to regulate fibroblast responses such as the expression of CTGF and COL-I.These results were also validated by in vivo studies.Therefore,this study proposed a valid self-adaptive strategy for ZnO improvement.Under different conditions,the sample could present different functions,and both the antibacterial ability and soft tissue compatibility were finely preserved. | Jin Leng Ye He Zhang Yuan Bailong Tao Ke Li Chuanchuan Lin Kun Xu Maowen Chen Liangliang Dai Xuemin Li Tony Jun Huang Kaiyong Cai | 2021 | Bioactive Materials2021，6，12： | 2 |
| 5 | Microfluidic diagnostics for the developing world显示文摘 | Xiaole Mao Tony Jun Huang | | Lab Chip 20]2 120，，： | 1 |
| 6 | Acoustofluidics for simultaneous nanopartide-based drug loading and exosome encapsulation显示文摘Nanocarrier and exosome encapsulation has been found to significantly increase the efficacy of targeted drug delivery while also minimizing unwanted side effects.However,the development of exosome-encapsulated drug nanocarriers is limited by low drug loading efficiencies and/or complex,time-consuming drug loading processes.Herein,we have developed an acoustofluidic device that simultaneously performs both drug loading and exosome encapsulation.By synergistically leveraging the acoustic radiation force,acoustic microstreaming,and shear stresses in a rotating droplet,the concentration,and fusion of exosomes,drugs,and porous silica nanoparticles is achieved.The final product consists of drug-loaded silica nanocarriers that are encased within an exosomal membrane.The drug loading efficiency is significantly improved,with nearly 30%of the free drug(e.g.,doxorubicin)molecules loaded into the nanocarriers.Furthermore,this acoustofluidic drug loading system circumvents the need for complex chemical modification,allowing drug loading and encapsulation to be completed within a matter of minutes.These exosome-encapsulated nanocarriers exhibit excellent efficiency in intracellular transport and are capable of significantly inhibiting tumor cell proliferation.By utilizing physical forces to rapidly generate hybrid nanocarriers,this acoustofluidic drug loading platform wields the potential to significantly impact innovation in both drug delivery research and applications. | Zeyu Wang Joseph Rich Nanjing Hao Yuyang Gu Chuyi Chen Shujie Yang Peiran Zhang Tony Jun Huang | 2022 | Microsystems & Nanoengineering2022，8，2： | 1 |
| 7 | Electrochemical micro-aptasensors for exosome detection based on hybridization chain reaction amplification显示文摘Exosomes are cell-derived nanovesicles that have recently gained popularity as potential biomarkers in liquid biopsies due to the large amounts of molecular cargo they carry,such as nucleic acids and proteins.However,most existing exosome-based analytical sensing methods struggle to achieve high sensitivity and high selectivity simultaneously.In this work,we present an electrochemical micro-aptasensor for the highly sensitive detection of exosomes by integrating a micropatterned electrochemical aptasensor and a hybridization chain reaction(HCR)signal amplification method.Specifically,exosomes are enriched on CD63 aptamer-functionalized electrodes and then recognized by HCR products with avidin-horseradish peroxidase(HRP)attached using EpCAM aptamers as bridges.Subsequently,the current signal that is generated through the enzyme reaction between the HRP enzyme and 3,3,,5/5,-tetramethylbenzidine(TMB)/H_(2)0_(2) directly correlates to the amount of bound HRP on the HCR products and thus to the number of target exosomes.By introducing anti-EpCAM aptamers,micro-aptasensors can detect cancerous exosomes with high specificity.Due to the micropatterned electrodes and HCR dual-amplification strategy,the micro-aptasensors achieve a linear detection response for a wide range of exosome concentrations from 2.5×10^(3) to 1×10^(7) exosomes/mL,with a detection limit of 5×10^(2) exosomes/mL.Moreover,our method successfully detects lung cancer exosomes in serum samples of early-stage and late-stage lung cancer patients,showcasing the great potential for early cancer diagnosis. | Wenfen Zhang Zhenhua Tian Shujie Yang Joseph Rich Shuaiguo Zhao Mikael Klingeborn Po-Hsun Huang Zhishang Li Alexander Stout Quinn Murphy Edward Patz Shusheng Zhang Guozhen Liu Tony Jun Huang | 2021 | Microsystems & Nanoengineering2021，7，4： | 1 |
| 8 | Energy-efficient digital and wireless IC design for wireless smart sensing显示文摘Wireless smart sensing is now widely used in various applications such as health monitoring and structural monitoring.In conventional wireless sensor nodes,significant power is consumed in wirelessly transmitting the raw data.Smart sensing adds local intelligence to the sensor node and reduces the amount of wireless data transmission via on-node digital signal processing.While the total power consumption is reduced compared to conventional wireless sensing,the power consumption of the digital processing becomes as dominant as wireless data transmission.This paper reviews the state-of-the-art energy-efficient digital and wireless IC design techniques for reducing the power consumption of the wireless smart sensor node to prolong battery life and enable self-powered applications. | Jun Zhou Xiongchuan Huang Chao Wang Tony Tae-Hyoung Kim Yong Lian | 2017 | Journal of Semiconductors2017，38，10： | 0 |
| 9 | An acoustofluidic scanning nanoscope using enhanced image stacking and processing显示文摘Nanoscale optical resolution with a large field of view is a critical feature for many research and industry areas,such as semiconductor fabrication,biomedical imaging,and nanoscale material identification.Several scanning microscopes have been developed to resolve the inverse relationship between the resolution and field of view;however,those scanning microscopes still rely upon fluorescence labeling and complex optical systems.To overcome these limitations,we developed a dual-camera acoustofluidic nanoscope with a seamless image merging algorithm(alphablending process).This design allows us to precisely image both the sample and the microspheres simultaneously and accurately track the particle path and location.Therefore,the number of images required to capture the entire field of view(200×200μm)by using our acoustofluidic scanning nanoscope is reduced by 55-fold compared with previous designs.Moreover,the image quality is also greatly improved by applying an alpha-blending imaging technique,which is critical for accurately depicting and identifying nanoscale objects or processes.This dual-camera acoustofluidic nanoscope paves the way for enhanced nanoimaging with high resolution and a large field of view. | Geonsoo Jin Joseph Rich Jianping Xia Albert JHe Chenglong Zhao Tony Jun Huang | 2022 | Microsystems & Nanoengineering2022，8，4： | 0 |
| 10 | 利用微流控技术应对COVID-19和其他肺部疾病的全球挑战显示文摘1. Introduction Pulmonary diseases present one of the most severe threats to human society. Since late 2019, the coronavirus disease 2019(COVID-19) pandemic has significantly impacted the lifestyle, culture, and politics of almost everyone in the world. COVID-19 causes severe pulmonary dysfunction, which is a major cause of mortality for those affected [1]. | Yuliang Xie Ryan Becker Michael Scott Kayla Bean Tony Jun Huang | 2023 | Engineering2023，，5： | 0 |
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