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| 1 | Hepatitis B and C virus-induced hepatitis: Apoptosis, autophagy, and unfolded protein response显示文摘AIM: To investigate the co-incidence of apoptosis, autophagy, and unfolded protein response(UPR) in hepatitis B(HBV) and C(HCV) infected hepatocytes.METHODS: We performed immunofluorescence confocal microscopy on 10 liver biopsies from HBV and HCV patients and tissue microarrays of HBV positive liver samples. We used specific antibodies for LC3β, cleaved caspase-3, BIP(GRP78), and XBP1 to detect autophagy, apoptosis and UPR, respectively. AntiHCV NS3 and anti-HBs antibodies were also used to confirm infection. We performed triple blind counting of events to determine the co-incidence of autophagy(LC3β punctuate), apoptosis(cleaved caspase-3), and unfolded protein response(GRP78) with HBV and HCV infection in hepatocytes. All statistical analyses were performed using SPSS software for Windows(Version 16 SPSS Inc, Chicago, IL, United States). P-values < 0.05 were considered statistically significant. Statistical analyses were performed with Mann-Whitney test to compare incidence rates for autophagy, apoptosis, and UPR in HBV- and HCV-infected cells and adjacent noninfected cells.RESULTS: Our results showed that infection of hepatocytes with either HBV and HCV induces significant increase(P < 0.001) in apoptosis(cleavage of caspase-3), autophagy(LC3β punctate), and UPR(increase in GRP78 expression) in the HCV- and HBVinfected cells, as compared to non-infected cells of the same biopsy sections. Our tissue microarray immunohistochemical expression analysis of LC3β in HBV^(Neg) and HBV^(Pos) revealed that majority of HBVinfected hepatocytes display strong positive stainingfor LC3β. Interestingly, although XBP splicing in HBVinfected cells was significantly higher(P < 0.05), our analyses show a slight increase of XBP splicing was in HCV-infected cells(P > 0.05). Furthermore, our evaluation of patients with HBV and HCV infection based on stage and grade of the liver diseases revealed no correlation between these pathological findings and induction of apoptosis, autophagy, and UPR.CONCLUSION: The results of this study indicate that HCV and HBV infection activates apoptosis, autophagy and UPR, but slightly differently by each virus. Further studies are warranted to elucidate the interconnections between these pathways in relation to pathology of HCV and HBV in the liver tissue. | Behzad Yeganeh Adel Rezaei Moghadam Javad Alizadeh Emilia Wiechec Seyed Moayed Alavian Mohammad Hashemi Bita Geramizadeh Afshin Samali Kamran Bagheri Lankarani Martin Post Payam Peymani Kevin M Coombs Saeid Ghavami | 2015 | World Journal of Gastroenterology2015，21，47： | 13 |
| 2 | A shield against a monster:Hepatitis C in hemodialysis patients显示文摘Hepatitis C virus(HCV)infection is highly prevalent among patients on hemodialysis(HD).The prevalence of HCV infection in HD patients varies markedly from country to country.Some factors are especially related to these high prevalence rates,such as blood transfusions and length of dialysis time. Nosocomial routes of transmission including the use of contaminated equipment and patient-to-patient exposure is considered more important.Several prophylactic measures have been suggested to avoid infection by HCV in the HD environment. | Seyed-Moayed Alavian | 2009 | World Journal of Gastroenterology2009，15，6： | 8 |
| 3 | Intra-familial prevalence of hepatitis B virologic markers in HBsAg positive family members in Nahavand, Iran显示文摘AIM: To determine the prevalence of hepatitis B in Nahavand and evaluate the HBsAg positive prevalence in families with a member who was confirmed to have HBV infection.METHODS: This study was performed in two phases. In the first phase, 1 824 subjects in Nahavand city were selected. The interviewers visited the houses of chosen families to fill the questionnaire and take the blood samples.All subjects signed an informed consent before interviews and blood sampling. The samples were evaluated for HBV virologic markers. In the second phase, 115 HBsAg-positive cases were enrolled and evaluated for HBV virologic markers.RESULTS: The prevalence of positive HBsAg in Nahavand was 2.3%. The most frequent relatives of index cases were sons and daughters (32.2% and 23.5% respectively).Twelve (11%) of all family members were HBsAg positive.Fifty (56.2%) were isolated HBsAb positive and only one person (2.5%) was isolated HBcAb positive. The higher rates of HBsAg marker were detected in the brothers (1-25%) and fathers (1-12.5%). The infection rate in husbands and wives of index cases was 10%. Only two (16.7%) of all HBsAg-positive participants reported previous HBV vaccination.CONCLUSION: The prevalence of intra-familial HBV infection is lower in Nahavand of Iran compared to other studies.More attention should be paid to HBV vaccination and risk-lowering activities. | Amir Houshang Mohammad Alizadeh Mitra Ranjbar Shahin Ansari Seyed Moayed Alavian Hamid Mohaghegh Shalmani Leila Hekmat Mohammad Reza Zali | 2005 | World Journal of Gastroenterology2005，11，31： | 8 |
| 4 | Prevalence of hepatitis C virus infection among hemodialysis patients in the Middle-East: A systematic review and meta-analysis显示文摘AIM To determine hepatitis C virus(HCV)infection prevalence in each country of the Middle-East and the overall prevalence of the region.METHODS In this systematic review,we gathered all documents related to HCV infection prevalence among hemodialysis patients in 17 middle-east countries from April 2006 to March 2016.We selected only cross-sectional studies that had proper sampling and measurement methods as well as a valid statistical analysis.RESULTS After screening of 7311 documents,56 studies were selected reporting the prevalence of HCV infection among hemodialysis patients from 10 countries of the region.Seven countries including United Arab Emirates,Afghanistan,Qatar,Bahrain,Kuwait,Oman,Israel,and Cyprus did not have any relevant document;thus,their latest reports were just mentioned.We performed the meta-analysis and determined the prevalence rates for each country as well as the whole region.The overall HCV infection prevalence among hemodialysis patients in the region was reported to be 25.3%;Egypt and Syria had the highest reported rates while Iran and Lebanon had the lowest.Further investigations are still needed to provide more reliable databases,find main risk factors,and to improve diagnosis and treatment plans,particularly in higher prevalent countries.CONCLUSION Controlling the prevalence and improving the management methods of HCV infection among hemodialysis patients are of a great concern in the Middle-East region. | Soheil Ashkani-Esfahani Seyed Moayed Alavian Mohammad Salehi-Marzijarani | 2017 | World Journal of Gastroenterology2017，23，1： | 7 |
| 5 | Prevalence of hepatitis C virus infection and its related risk factors in drug abuser prisoners in Hamedan-Iran显示文摘AIM: Recent studies in Iran has shown that prevalence of hepatitis C virus (HCV) infection among Iranian prisoners is high, in spite of low HCV seroprevalence in general population.METHODS: This study was carried out in the central prison of Hamedan - Iran, in year 2002. Inmates were interviewed using a standard questionnaire including demographic,imprisonment history and HCV-related risk behaviors items. Thereafter, the sera drawn from the participants were tested for anti-HIV and anti-HCV antibodies.RESULTS: A total number of 427 drug abuser inmates participated in our study. Three hundred and ninety-seven (93%) were men and 30 (7%) were women. Total number of Ⅳ drug abusers (IDA) and non-Ⅳ drug abusers (NIDA)was 149 (34.9%) and 278 (65.1%), respectively. The overall rate of antibody positivity among inmates was 0.9% for HIV and 30% for HCV. Of all IDAs, 31.5% and of NIDAs, 29.1% had serological evidence of HCV infection.CONCLUSION: The seroprevalence of HCV infection among drug abuser prisoners in comparison with the general population in Iran, is very high (30% vs in italics 0.2%).Our results indicate the importance of policies to prevent transmission of HCV infection during and following incarceration. | Amir Houshang Mohammad Alizadeh Seyed Moayed Alavian Khalil Jafari Nastaran Yazdi | 2005 | World Journal of Gastroenterology2005，11，26： | 5 |
| 6 | Elevated alanine aminotransferase activity is not associated with dyslipidemias,but related to insulin resistance and higher disease grades in non-diabetic non-alcoholic fatty liver disease显示文摘Objective:To explore demographic and metabolic factors associated with increased alanine aminotransferase(ALT)activity in non-diabetic non-alcoholic fatty liver disease(NAFLD)patients.Methods:Overall 372 patients who consecutively attended to Gastroenterology Clinic of Baqiyatallah University of Medical Sciences,Tehran,Iran awere diagnosed as NAFLD entered into analysis.Exclusion criteria were having diabetes mellitus and fasting blood glucose over126 mg/dL,active hepatitis B virus infection,having hepatitis C virus positive serology,and to be under corticosteroid therapy.ALT levels were considered pathologically high when it was over30 IU/L for men and over 19 IU/L for women.Results:Bivariate analyses using t test and chisquare test showed that patients with pathologically augmented ALT levels had significantly higher NAFLD grades in their ultrasonographic evaluations(P=0.003).Moreover,these patients represented significantly higher homeostatic model assessment levels(P=0.003),levels of serum insulin(P=0.002),fasting blood glucose(P<0.001),and uric acid(P=0.02).The prevalence of insulin resistance was also higher in patients with increased serum ALT concentrations.Multifactorial logistic regression models showed that ultrasonographic grading of NAFLD(P=0.027)and insulin resistance(P=0.013)were the only variables significantly associated with abnormal ALT levels.Conclusions:This study shows that the associations of increased ALT serum levels in NAFLD patients are different from what are supposed before.By excluding diabetic patients from our population,we find that increased ALT levels are not associated with dyslipidemias but are independently associated with insulin resistance and NAFLD grading on ultrasonographic evaluations.Further studies are needed to confirm our results. | Mohammad Ebrahim Ghamar-Chehreh Mohsen Amini Hossein Khedmat Seyed Moayed Alavian Fatemeh Daraei Reza Mohtashami Reza Hadi Bent-Al-Hoda Beyram Saeed Taheri | 2012 | Asian Pacific Journal of Tropical Biomedicine2012，2，9： | 5 |
| 7 | Non-alcoholic steatohepatitis and influence of age and gender on histopathologic findings显示文摘AIM:To characterize the histopathologic specifications of non-alcoholic steatohepatitis(NASH)according to age and gender.METHODS:An analytical cross-sectional study was conducted in two private gastroenterology clinics on biopsy proven patients suffering from NASH.Biopsy histopathologic findings as well as demographic and laboratory data of the patients at the time of biopsy were gathered retrospectively from clinical records.The grading and staging of histopathologic findings were performed according to the Brunt method after reevaluation of the slides by a pathologist.Patients were divided into two groups according to age(belowand above 55 years).Mean quantitative grade of all pathologic findings were also calculated according to Brunt scoring values.RESULTS:A total number of 77 NASH patients,consisting of 58 males(75.3%)and 19(24.7%)females with a mean age of 41.99±11.80 years(range,18-70 years),were enrolled.The mean age(48.72±13.99 years vs 39.74±10.16 years,P=0.004)and aspartate aminotransferase level(75.11±29.68 U/L vs 52.78± 25.00 U/L,P=0.002)was significantly higher in female patients.Mean quantitative grade of hepatosteatosis was significantly higher in females(2.00±0.82 vs 1.59 ±0.68,P=0.031)compared to males.Fifty four percent(34/65)of young patients had mild hepatosteatosis (GradeⅠ)while only one patient(11.2%)in the older group had gradeⅠhepatosteatosis.Patients aged≥55 had significantly more severe hepatosteatosis(GradeⅢ) (44.4%vs 9.5%,P=0.007)and the mean quantitative grade of hepatosteatosis was significantly higher among them(2.33±0.71 vs 1.56±0.67,P=0.002).Multivariate analysis after omitting the confounding role of age revealed a higher grade of hepatosteatosis in female patients(P=0.010).CONCLUSION:These findings point toward the possible influence of age in the severity of steatohepatitis,portal and lobar inflammation in patients suffering from NASH while gender independently might contribute to the level of steatohepatitis. | Nargess Ebrahimi Daryani Nasser Ebrahimi Daryani Seyed Moayed Alavian Ali Zare Seyed-Mohammad Fereshtehnejad Mohammad Reza Keramati Mohammad Reza Pashaei Peiman Habibollahi | 2010 | World Journal of Gastroenterology2010，16，33： | 3 |
| 8 | Regulatory T cells are the most important determinant factor of hepatitis B infection prognosis: A systematic review and meta-analysis显示文摘 | Seyed Hossein Aalaei-Andabili Seyed Moayed Alavian | 2012 | Vaccine2012，，38： | 3 |
| 9 | Molecular epidemiology of hepatitis C virus among injection drug users in Iran: a slight change in prevalence of HCV genotypes over time显示文摘 | Katayoun Samimi-Rad Mohsen Nasiri Toosi Ali Masoudi-nejad Ali Najafi Ramin Rahimnia Fatemeh Asgari Alireza Namazi Shabestari Gholamreza Hassanpour Seyed-Moayed Alavian Freshteh Asgari | 2012 | Archives of Virology2012，，10： | 2 |
| 10 | Preliminary report of hepatitis B virus genotype prevalence in Iran显示文摘瞄准:决定在伊朗的肝炎 B 的肝炎 B (HBV ) 遗传型的流行出现抗原(HBsAg ) 搬运人,长期的肝炎 B 和肝脏硬化症的病人。方法:109 个 HBsAg 积极的病人的一个总数在这研究被包括。HBV 遗传型被使用基于反向的杂交原则的 INNO-LiPA 方法论决定。结果:有肝疾病的不同阶段的病人的分发如下:95 (86.4%) 长期的肝炎, 11 (10%) 肝肝硬化,并且 3 (2.7%) 不活跃的搬运人。长期的肝炎和肝肝硬化病人,当 70% 是 HBeAg 否定的时, 26.4% 是 HBeAg 积极的。遗传型 D 是唯一的在所有病人发现的检测类型。结论:分类 HBV 进遗传型不得不划算、临床上相关。我们的学习显示 HBV 遗传型 D 在地中海区域占优势,在附近并且中东,和南亚。为通过国际合作理解 HBV 遗传型的继续的努力将揭示遗传型和他们的临床的关联的进一步的 virological 差别。 | Seyed-Moayed Alavian Hossein Keyvani Mahdi Rezai Neda Ashayeri Homa Mohammad Sadeghi | 2006 | World Journal of Gastroenterology2006，12，32： | 2 |
| 11 | Therapeutic potential of RNA interference:a new molecular approach to antiviral treatment for hepatitis C显示文摘 | Motavaf M Safari S Alavian SM | 2012 | J Viral Hepat2012，19，11： | 1 |
| 12 | Transcriptional regulation of mesencephalic dopaminergic neurons: the full circle of life and Death显示文摘 | Alavian KN Scholz C Simon HH | 2008 | Mov Disord2008，23，： | 1 |
| 13 | Hepatitis C in hemodialysis patients:current global magnitude, natural history, diagnostic difficulties, and preventive measures 显示文摘 | Rahnavardi M Hosseini Moghaddam SM Alavian SM | 2008 | Am J Nephrol2008，28，4： | 1 |
| 14 | Molecular characterization of hepatitis B virus (HBV) strains circulating in the northern coast of the Persian Gulf and its comparison with worldwide distribution of HBV subgenotype D1显示文摘 | Mahmoud Reza Pourkarim Valentijn Vergote Samad Amini‐Bavil‐Olyaee Zohre Sharifi Steven Sijmons Philippe Lemey Piet Maes Seyed Moayed Alavian Marc Van Ranst | 2014 | Virol2014，，5： | 1 |
| 15 | Insuljn Resistance in chronic Hepatitjs B and C 显示文摘 | Mohammad Alizadeh AH Fallahian F Alavians M | 2006 | IndianJ Gastroenterol2006，25，6： | 1 |
| 16 | Behavior of density on an incline显示文摘 | ALAVIAN V | 1986 | Journal of Hydraulic Engineering1986，112，1： | 1 |
| 17 | Density currents entering lakes and reservoirs 显示文摘 | ALAVIAN V JIRKA G H DENTON R A | 1992 | Journal of Hy-draulic Engineering1992，118，1： | 1 |
| 18 | The effi- cacy of Liv-52 on liver cirrhotic patients: arandomized, double-blind, placebo-controlled first approach 显示文摘 | Huseini HF Alavian SM Heshmat R | 2005 | Phytomedicine2005，12，9： | 1 |
| 19 | Virus- triggered autophagy in viral hepatitis-possible novel strategies for drug development显示文摘 | ALAVIAN S M ANDES R COOMBS K M | 2011 | Journal of Viral Hepatitis2011，18，12： | 1 |
| 20 | The efficacy of Liv-52 on liver cirrhotic patients: A randomized, double-blind, placebo-controlled first approach显示文摘 | Huseini H F Alavian S M Heshmat R | 2005 | Phytomedicine2005，12，： | 1 |
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