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| 1 | Aging related methylation influences the gene expression of key control genes in colorectal cancer and adenoma显示文摘AIM To analyze colorectal carcinogenesis and age-related DNA methylation alterations of gene sequences associated with epigenetic clock CpG sites. METHODS In silico DNA methylation analysis of 353 epigenetic clock Cp G sites published by Steve Horvath was performed using methylation array data for a set of 123 colonic tissue samples [64 colorectal cancer(CRC), 42 adenoma, 17 normal; GEO accession number: GSE48684]. Among the differentially methylated agerelated genes, secreted frizzled related protein 1(SFRP1) promoter methylation was further investigated in colonic tissue from 8 healthy adults, 19 normal children, 20 adenoma and 8 CRC patients using bisulfite-specific PCR followed by methylation-specific high resolution melting(MS-HRM) analysis. m RNA expression of age-related 'epigenetic clock' genes was studied using Affymetrix HGU133 Plus2.0 whole transcriptome data of 153 colonic biopsy samples(49 healthy adult, 49 adenoma, 49 CRC, 6 healthy children)(GEO accession numbers: GSE37364, GSE10714, GSE4183, GSE37267). Whole promoter methylation analysis of genes showing inverse DNA methylationgene expression data was performed on 30 colonic samples using methyl capture sequencing.RESULTS Fifty-seven age-related Cp G sites including hypermethylated PPP1R16 B, SFRP1, SYNE1 and hypomethylated MGP, PIPOX were differentially methylated between CRC and normal tissues(P < 0.05, ?β≥ 10%). In the adenoma vs normal comparison, 70 CpG sites differed significantly, including hypermethylated DKK3, SDC2, SFRP1, SYNE1 and hypomethylated CEMIP, SPATA18(P < 0.05, ?β≥ 10%). In MS-HRM analysis, the SFRP1 promoter region was significantly hypermethylated in CRC(55.0% ± 8.4 %) and adenoma tissue samples(49.9% ± 18.1%) compared to normal adult(5.2% ± 2.7%) and young(2.2% ± 0.7%) colonic tissue(P < 0.0001). DNA methylation of SFRP1 promoter was slightly, but significantly increased in healthy adults compared to normal young samples(P < 0.02). This correlated with significantly increased SFRP1 m RNA levels in children compared to normal adult samples(P < 0.05). In CRC tissue the mR NA expression of 117 agerelated genes were changed, while in adenoma samples 102 genes showed differential expression compared with normal colonic tissue(P < 0.05, logF C > 0.5). The change of expression for several genes including SYNE1, CLEC3 B, LTBP3 and SFRP1, followed the same pattern in aging and carcinogenesis, though not for all genes(e.g., MGP). CONCLUSION Several age-related DNA methylation alterations can be observed during CRC development and progression affecting the m RNA expression of certain CRC- and adenoma-related key control genes. | Orsolya Galamb Alexandra Kalmár Barbara Kinga Barták árpád V Patai Katalin Leiszter Bálint Péterfia Barnabás Wichmann Gábor Valcz Gábor Veres Zsolt Tulassay Béla Molnár | 2016 | World Journal of Gastroenterology2016，22，47： | 7 |
| 2 | Pancreatic cancer: Are 'liquid biopsies' ready for prime-time?显示文摘Pancreatic cancer is a disease that carries a poor prognosis. Accurate tissue diagnosis is required. Tumours contain a high content of stromal tissue and therefore biopsies may be inconclusive. Circulating tumour cells(CTCs) have been investigated as a potential 'liquid biopsy' in several malignancies and have proven to be of prognostic value in breast, prostate and colorectal cancers. They have been detected in patients with localised and metastatic pancreatic cancer with sensitivities ranging from 38%-100% using a variety of platforms. Circulating tumour DNA(ct DNA) has also been detected in pancreas cancer with a sensitivity ranging from 26%-100% in studies across different platforms and using different genetic markers. However, there is no clear consensus on which platform is the most effective for detection, nor which genetic markers are the most useful to use. Potential roles of liquid biopsies include diagnosis, screening, guiding therapies and prognosis. The presence of CTCs or ct DNA has been shown to be of prognostic value both at diagnosis and after treatment in patients with pancreatic cancer. However, more prospective studies are required before this promising technology is ready for adoption into routine clinical practice. | Alexandra R Lewis Juan W Valle Mairead G Mc Namara | 2016 | World Journal of Gastroenterology2016，22，32： | 5 |
| 3 | Arterial percutaneous angioplasty in upper limbs with vascular access devices for haemodialysis 显示文摘 | Alexandra G Alain R Bernard B | 2002 | Nephrol Dial Transplant2002，17，5： | 1 |
| 4 | Structure-toxicity relationships of nitroaromatic compounds显示文摘 | ALEXANDRA I BAKHTIYOR R LEONID G | 2006 | Molecular Diversity2006，10，3： | 1 |
| 5 | Meta-Analysis of Risedronate for the Treatment of Postmenopausal Osteoporosis显示文摘 | Ann C Peter T Jonathan A Bruce W Nicole Z Alexandra P Vivian R Beverley S George W Gordon G | 2002 | Endocrine Reviews2002，23，4： | 1 |
| 6 | Height, weight,weight change, and ovarian cancer risk in the Netherlands cohort study on diet and cancer显示文摘 | Schouten L J Alexandra G R Brandt P A | 2003 | Am J Epidemiol2003，157，5： | 1 |
| 7 | Synthesis,properities and biomedical applications of poly (glycerol sebacate) (PGS):A review显示文摘 | Ranjana R Marwa T Alexandra G | 2012 | Progress in Polymer Science2012，37，： | 1 |
| 8 | Management of malignant bowel obstruction 显示文摘 | Carlaida R Alexandra ME Hans G | 2008 | European Journal of Cancer2008，44，： | 1 |
| 9 | Association of Low Striatal Dopamine D^sub 2^ Receptor Availability With Nicotine Dependence Similar to That Seen With Other Drugs of Abuse显示文摘 | Fehr Christoph Yakushev Igor Hohmann Nina Buchholz Hans-Georg Landvogt Christian Deckers Hanna Eberhardt Alexandra Kl?ger Marie Smolka Michael N Scheurich Armin Dielentheis Thomas Schmidt Lutz G R?sch Frank Bartenstein Peter Gründer Gerhard | 2008 | The American Journal of Psychiatry2008，，4： | 1 |
| 10 | Electrokinetic remediation of organochlorines in soil: Enhancement techniques and integration with other remediation technologies显示文摘 | Helena I G Celia D F Alexandra B R | 2012 | Chemosphere2012，87，10： | 1 |
| 11 | Characterization of 'basparin A,'a prothrombin-activating metalloproteinase,from the venom of the snake Bothrops asper that inhibits platelet aggregation and induces defibrination and thrombosis显示文摘 | Loria G D Alexandra R | 2003 | Biochemistry and Biophysics2003，418，1： | 1 |
| 12 | Evaluation of some mathematical models for tumor growth显示文摘 | FRANK J ALEXANDRA J R | 1982 | Int J Bio-Med Comput1982，，13： | 1 |
| 13 | Prevalence, Clear-ance, and Incidence of anal human papillomavirus infection inHIV-Infected men: The HIPVIRG Cohort Study 显示文摘 | Alexandra P Danielle R George G | 2009 | J InfectDis2009，199，： | 1 |
| 14 | Highly dispersed ceria on activated carbon for the catalyzed ozonation of organic pollutants 显示文摘 | Alexandra G Joaqui S Enrique V R | 2012 | Applied Catalysis B: Environmental2012，113，114： | 1 |
| 15 | Continuous analog of the Gauss-Newton method 显示文摘 | RUBEN G A ALEXANDER G R ALEXANDRA B S | 1999 | Mathematical Models and Methods in Applied Sciences1999，9，3： | 1 |
| 16 | Overlap syndrome of primary biliary cirrhosis and autoimmune hepatitis: a retrospective study of 115 cases of autoimmune liver disease显示文摘 | Alexandra Heurgué Fabien Vitry Marie-Danièle Diebold Nahla Yaziji Brigitte Bernard-Chabert Jean-Loup Pennaforte Rémi Picot Hervé Louvet Luc Frémond Patrick Geoffroy Jean-Luc Schmit Guillaume Cadiot Gérard Thiéfin | 2007 | Gastroenterologie Clinique et Biologique2007，，： | 1 |
| 17 | Overlap syndrome of primary biliary cirrhosis and autoimmune hepatitis: a retrospective study of 115 cases of autoimmune liver disease显示文摘 | Alexandra Heurgué Fabien Vitry Marie-Danièle Diebold Nahla Yaziji Brigitte Bernard-Chabert Jean-Loup Pennaforte Rémi Picot Hervé Louvet Luc Frémond Patrick Geoffroy Jean-Luc Schmit Guillaume Cadiot Gérard Thiéfin | 2007 | Gastroenterologie Clinique et Biologique2007，，1： | 1 |
| 18 | Role of the cytoplasmic tail of pseudorabies virus glycoprotein E in virion formation 显示文摘 | Alexandra R B Barbara G K Harald G | 2000 | J Virol2000，74，9： | 1 |
| 19 | Health-related quality of life, anxiety, depression and impulsivity in patients with advanced gastroenteropancreatic neuroendocrine tumours显示文摘AIM To compare health-related quality of life(HRQoL),anxiety, depression, and impulsivity scores in patients with and without carcinoid syndrome(CS), and correlated them with serum 5-hydroxyindoleacetic acid(5-HIAA) levels.METHODS Patients with advanced gastroenteropancreatic neuroendocrine tumours(GEPNET), with and without CS completed HRQoL QLQ-C30 and QLQ-GI.NET21, Hospital Anxiety and Depression Scale(HADS) and Barratt Impulsivity Scale(BIS) questionnaires. Twosample Wilcoxon test was applied to assess differences in serum 5-HIAA levels, two-sample Mann-Whitney U test for HRQoL and BIS, and proportion test for HADS, between those with and without CS.RESULTS Fifty patients were included; 25 each with and without CS. Median 5-HIAA in patients with and without CS was 367 nmol/L and 86 nmol/L, respectively(P = 0.003). Scores related to endocrine symptoms were significantly higher amongst patients with CS(P = 0.04) and scores for disease-related worries approached significance in the group without CS, but no other statistically-significant differences were reported between patients with and without CS in responses on QLQ-C30 or QLQ-GI.NET21. Fifteen patients(26%) scored ≥ 8/21 on anxiety scale, and 6(12%) scored ≥ 8/21 on depression scale. There was no difference in median 5-HIAA between those scoring < or ≥ 8/21 on anxiety scale(P = 0.53). There were no statistically significant differences between groups in first or second-order factors(BIS) or total sum(P = 0.23).CONCLUSION Excepting endocrine symptoms, there were no significant differences in HRQoL, anxiety, depression or impulsivity between patients with advanced GEPNET, with or without CS. Over one quarter of patients had high anxiety scores, unrelated to peripheral serotonin metabolism. | Alexandra R Lewis Xin Wang Laurice Magdalani Paolo D'Arienzo Colsom Bashir Was Mansoor Richard Hubner Juan W Valle Mairéad G McNamara | 2018 | World Journal of Gastroenterology2018，24，6： | 1 |
| 20 | Agility and Perturbation Training Techniques in Exercise Therapy for Reducing Pain and Improving Function in People With Knee Osteoarthritis: A Randomized Clinical Trial显示文摘 | Fitzgerald G Kelley Piva Sara R Gil Alexandra B Wisniewski Stephen R Oddis Chester V Irrgang James J | 2011 | Physical Therapy2011，，4： | 1 |
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